Laser Light Scattering
By Charles S. Johnson and Don A. Gabriel
5/5
()
About this ebook
This volume, which employs a relatively simple approach in order to reach the widest audience, focuses on two main topics: classical light scattering (scattering intensity, concentration dependence, size dependence, and polydispersity) and dynamic light scattering (time and frequency dependence, translational diffusion, directed flow, rotational motion, and more). A series of useful appendixes and a list of references complete this concise, accessible work, a valuable resource for physicists, chemists, and anyone interested in the increasingly important field of laser light scattering.
Read more from Charles S. Johnson
Problems and Solutions in Quantum Chemistry and Physics Rating: 5 out of 5 stars5/5
Related to Laser Light Scattering
Titles in the series (100)
The Theory of Heat Radiation Rating: 3 out of 5 stars3/5Quantum Mechanics with Applications Rating: 2 out of 5 stars2/5Problems in Quantum Mechanics: Third Edition Rating: 3 out of 5 stars3/5A History of Mechanics Rating: 4 out of 5 stars4/5Electronic Structure and the Properties of Solids: The Physics of the Chemical Bond Rating: 3 out of 5 stars3/5Rational Mechanics: The Classic Notre Dame Course Rating: 5 out of 5 stars5/5Theory of Linear Physical Systems: Theory of physical systems from the viewpoint of classical dynamics, including Fourier methods Rating: 0 out of 5 stars0 ratingsStatistical Fluid Mechanics, Volume II: Mechanics of Turbulence Rating: 0 out of 5 stars0 ratingsBrownian Movement and Molecular Reality Rating: 0 out of 5 stars0 ratingsEquilibrium Statistical Mechanics Rating: 4 out of 5 stars4/5Light Rating: 4 out of 5 stars4/5Group Theory in Quantum Mechanics: An Introduction to Its Present Usage Rating: 0 out of 5 stars0 ratingsGravitational Curvature: An Introduction to Einstein's Theory Rating: 0 out of 5 stars0 ratingsTreatise on Physiological Optics, Volume III Rating: 0 out of 5 stars0 ratingsMathematics of Relativity Rating: 0 out of 5 stars0 ratingsAn Elementary Treatise on Theoretical Mechanics Rating: 5 out of 5 stars5/5An Introduction to Acoustics Rating: 1 out of 5 stars1/5Water Waves: The Mathematical Theory with Applications Rating: 0 out of 5 stars0 ratingsA First Look at Perturbation Theory Rating: 4 out of 5 stars4/5Symmetry: An Introduction to Group Theory and Its Applications Rating: 4 out of 5 stars4/5The Philosophy of Space and Time Rating: 5 out of 5 stars5/5Readable Relativity Rating: 4 out of 5 stars4/5Dynamic Light Scattering: With Applications to Chemistry, Biology, and Physics Rating: 5 out of 5 stars5/5An Elementary Survey of Celestial Mechanics Rating: 0 out of 5 stars0 ratingsGeneral Relativity and Gravitational Waves Rating: 5 out of 5 stars5/5Quantum Mechanics of One- and Two-Electron Atoms Rating: 0 out of 5 stars0 ratingsThermoelectricity: An Introduction to the Principles Rating: 4 out of 5 stars4/5Theories of Figures of Celestial Bodies Rating: 0 out of 5 stars0 ratingsIntroduction to Electromagnetic Theory Rating: 0 out of 5 stars0 ratingsGet a Grip on Physics Rating: 3 out of 5 stars3/5
Related ebooks
Introduction to Modern Optics Rating: 4 out of 5 stars4/5Foundations of Laser Spectroscopy Rating: 0 out of 5 stars0 ratingsOptical Resonance and Two-Level Atoms Rating: 3 out of 5 stars3/5The Method of Second Quantization Rating: 0 out of 5 stars0 ratingsWave Propagation in a Random Medium Rating: 0 out of 5 stars0 ratingsDynamic Light Scattering: With Applications to Chemistry, Biology, and Physics Rating: 5 out of 5 stars5/5Molecular Vibrations: The Theory of Infrared and Raman Vibrational Spectra Rating: 0 out of 5 stars0 ratingsQuantum Theory: Radiation and High Energy Physics Rating: 5 out of 5 stars5/5X-Ray Diffraction: In Crystals, Imperfect Crystals, and Amorphous Bodies Rating: 0 out of 5 stars0 ratingsGroup Theory and Quantum Mechanics Rating: 4 out of 5 stars4/5Plasma Physics Rating: 0 out of 5 stars0 ratingsConcepts of Classical Optics Rating: 0 out of 5 stars0 ratingsMicrowave Spectroscopy Rating: 2 out of 5 stars2/5Concepts of Quantum Optics Rating: 5 out of 5 stars5/5Ideas of Quantum Chemistry: Volume 2: Interactions Rating: 4 out of 5 stars4/5Physics of Waves Rating: 5 out of 5 stars5/5Light Scattering by Particles in Water: Theoretical and Experimental Foundations Rating: 0 out of 5 stars0 ratingsClassical Electricity and Magnetism: Second Edition Rating: 4 out of 5 stars4/5Spins in Chemistry Rating: 3 out of 5 stars3/5Foundations of Statistical Mechanics: A Deductive Treatment Rating: 0 out of 5 stars0 ratingsIntroduction to Infrared and Raman Spectroscopy Rating: 0 out of 5 stars0 ratingsX-Ray Diffraction Rating: 5 out of 5 stars5/5Introduction to Nonlinear Laser Spectroscopy 2e Rating: 0 out of 5 stars0 ratingsLight Rating: 4 out of 5 stars4/5Principles of Thermodynamics and Statistical Mechanics Rating: 0 out of 5 stars0 ratingsQuantum Mechanics: Principles and Formalism Rating: 0 out of 5 stars0 ratingsThe Theory of Heat Radiation Rating: 3 out of 5 stars3/5Molecules and Radiation: An Introduction to Modern Molecular Spectroscopy. Second Edition Rating: 5 out of 5 stars5/5Foundations of Radiation Hydrodynamics Rating: 4 out of 5 stars4/5Optical Sources, Detectors, and Systems: Fundamentals and Applications Rating: 0 out of 5 stars0 ratings
Physics For You
Physics I For Dummies Rating: 4 out of 5 stars4/5How to Diagnose and Fix Everything Electronic, Second Edition Rating: 4 out of 5 stars4/5The Reality Revolution: The Mind-Blowing Movement to Hack Your Reality Rating: 4 out of 5 stars4/5String Theory For Dummies Rating: 4 out of 5 stars4/5Physics Essentials For Dummies Rating: 4 out of 5 stars4/5What If?: Serious Scientific Answers to Absurd Hypothetical Questions Rating: 5 out of 5 stars5/5Step By Step Mixing: How to Create Great Mixes Using Only 5 Plug-ins Rating: 5 out of 5 stars5/5Quantum Physics: A Beginners Guide to How Quantum Physics Affects Everything around Us Rating: 5 out of 5 stars5/5AP Physics 1 Premium, 2024: 4 Practice Tests + Comprehensive Review + Online Practice Rating: 0 out of 5 stars0 ratingsUnlocking Spanish with Paul Noble Rating: 5 out of 5 stars5/5Quantum Physics for Beginners Rating: 4 out of 5 stars4/5Nuclear Physics Rating: 4 out of 5 stars4/5Moving Through Parallel Worlds To Achieve Your Dreams Rating: 4 out of 5 stars4/5The God Effect: Quantum Entanglement, Science's Strangest Phenomenon Rating: 4 out of 5 stars4/5How to Teach Quantum Physics to Your Dog Rating: 4 out of 5 stars4/5Feynman Lectures Simplified 1A: Basics of Physics & Newton's Laws Rating: 5 out of 5 stars5/5Welcome to the Universe: An Astrophysical Tour Rating: 4 out of 5 stars4/5The Dancing Wu Li Masters: An Overview of the New Physics Rating: 4 out of 5 stars4/5God Particle: If the Universe Is the Answer, What Is the Question? Rating: 5 out of 5 stars5/5QED: The Strange Theory of Light and Matter Rating: 4 out of 5 stars4/5Beyond Weird: Why Everything You Thought You Knew about Quantum Physics Is Different Rating: 4 out of 5 stars4/5What the Bleep Do We Know!?™: Discovering the Endless Possibilities for Altering Your Everyday Reality Rating: 5 out of 5 stars5/5The Little Book of String Theory Rating: 4 out of 5 stars4/5Complexity: The Emerging Science at the Edge of Order and Chaos Rating: 4 out of 5 stars4/5A Universe from Nothing: Why There Is Something Rather than Nothing Rating: 4 out of 5 stars4/5The Theory of Relativity: And Other Essays Rating: 4 out of 5 stars4/5The Grid: The Fraying Wires Between Americans and Our Energy Future Rating: 4 out of 5 stars4/5
Reviews for Laser Light Scattering
1 rating0 reviews
Book preview
Laser Light Scattering - Charles S. Johnson
DOVER BOOKS ON PHYSICS
EXACTLY SOLVED MODELS IN STATISTICAL MECHANICS, R. J. Baxter. (0-486-46271-4)
QUANTUM MECHANICS OF ONE- AND TWO-ELECTRON ATOMS, Hans A. Bethe and Edwin E. Salpeter. (0-486-46667-1)
PHYSICAL ADSORPTION: FORCES AND PHENOMENA, L. W. Bruch, Milton W. Cole and Eugene Zaremba. (0-486-45767-2)
LASER LIGHT SCATTERING: BASIC PRINCIPLES AND PRACTICE. SECOND EDITION, Benjamin Chu. (0-486-45798-2)
ALGEBRAIC METHODS IN STATISTICAL MECHANICS AND QUANTUM FIELD THEORY, Dr. Gérard G. Emch. (0-486-47209-4)
INTRODUCTION TO MODERN OPTICS, Grant R. Fowles. (0-486-65957-7)
WEAK INTERACTIONS AND MODERN PARTICLE THEORY, Howard Georgi. (0-486-46904-2)
COLLISION THEORY, Marvin L. Goldberger and Kenneth M. Watson. (0-486-43507-5)
GROUP THEORY IN QUANTUM MECHANICS: AN INTRODUCTION TO ITS PRESENT USAGE, Volker Heine. (0-486-45878-4)
CONDENSED MATTER PHYSICS, Akira Isihara. (0-486-45877-6)
QUANTUM FIELD THEORY, Claude Itzykson and Jean-Bernard Zuber. (0-486-44568-2)
THEORETICAL PHYSICS, Georg Joos and Ira M. Freeman. (0-486-65227-0)
QUANTUM MECHANICS IN SIMPLE MATRIX FORM, Thomas F. Jordan. (0-486-44530-5)
OPERATORS AND REPRESENTATION THEORY: CANONICAL MODELS FOR ALGEBRAS OF OPERATORS ARISING IN QUANTUM MECHANICS, Palle E. T. Jorgensen. (0-486-46665-5)
FUNDAMENTALS OF MATHEMATICAL PHYSICS, Edgar A. Kraut. (0-486-45809-1)
QUANTUM MECHANICS: NEW APPROACHES TO SELECTED Topics, Harry J. Lipkin. (0-486-45893-8)
A TREATISE ON THE MATHEMATICAL THEORY OF ELASTICITY (EOU), . (0-486-46425-3)
STOCHASTIC TOOLS IN TURBULENCE, John L. Lumley. (0-486-46270-6)
A TREATISE ON ELECTRICITY AND MAGNETISM, VOL. 1, James Clerk Maxwell. (0-486-60636-8)
A TREATISE ON ELECTRICITY AND MAGNETISM, VOL. 2, James Clerk Maxwell. (0-486-60637-6)
QUANTUM MECHANICS, Albert Messiah. (0-486-40924-4)
STATISTICAL FLUID MECHANICS, VOLUME II: MECHANICS OF TURBULENCE, A. S. Monin and A. M. Yaglom. (0-486-45891-1)
STATISTICAL FLUID MECHANICS, VOLUME I: MECHANICS OF TURBULENCE, A. S. Monin and A. M. Yaglom. (0-486-45883-0)
SINGULAR INTEGRAL EQUATIONS: BOUNDARY PROBLEMS OF FUNCTION THEORY AND THEIR APPLICATION TO MATHEMATICAL PHYSICS, N. I. Muskhelishvili. Translated by J. R. M. Radok. (0-486-46242-0)
INTRODUCTION TO THE QUANTUM THEORY: THIRD EDITION, David Park. (0-486-44137-7)
APPLICATIONS OF GROUP THEORY IN QUANTUM MECHANICS, M. I. Petrashen, E. D. Trifonov. (0-486-47223-X)
ALMOST ALL ABOUT WAVES, John R. Pierce. (0-486-45302-2)
CLASSICAL FIELD THEORY, Davison E. Soper. (0-486-46260-9)
CONCEPTS OF CLASSICAL OPTICS, John Strong. (0-486-43262-9)
THE LIGHTNING DISCHARGE, Martin A. Uman. (0-486-41463-9)
SOURCES OF QUANTUM MECHANICS, B. L. van der Waerden. (0-486-45892-X)
SPACE, TIME, MATTER, Hermann Weyl. (0-486-60267-2)
RANDOM VIBRATIONS: THEORY AND PRACTICE, Paul H. Wirsching, Thomas L. Paez and Keith Ortiz. (0-486-45015-5)
VARIATIONAL PRINCIPLES IN DYNAMICS AND QUANTUM THEORY, Wolfgang Yourgrau and Stanley Mandelstam. (0-486-45888-1)
PHYSICS OF SHOCK WAVES AND HIGH-TEMPERATURE HYDRODYNAMIC PHENOMENA, Ya B. Zel’dovich and Yu P. Raizer. (0-486-42002-7)
See every Dover book in print at www.doverpublications.com
Copyright
Copyright © 1981 by CRC Press, Inc. All rights reserved.
Bibliographical Note
Laser Light Scattering was originally published in Spectroscopy in Biochemistry, Volume II, edited by J. Ellis Bell, copyright 1981. Reprinted with the permission of CRC Press, Boca Raton, Florida. This Dover edition, first published in 1994, is corrected and includes a new Preface.
Library of Congress Cataloging-in-Publication Data
Johnson, Charles S. (Charles Sidney), 1936-
Laser light scattering / Charles S. Johnson, Jr. and Don A. Gabriel.
p. cm.
An unabridged, corrected republication of the work first published as chapter 5 of the book Spectroscopy in biochemistry (volume II), edited by J. Ellis Bell, CRC Press, Boca Raton, Florida, 1981, with a new preface.
Includes bibliographical references (p. ).
9780486157733
1. Laser spectroscopy. 2. Laser beams—Scattering. 3. Lasers in biochemistry. I. Gabriel, Don A. II. Title.
QP519.9.L37J64 1994
674.19’285—dc20
94-24205
CIP
Manufactured in the United States by Courier Corporation
68328105
www.doverpublications.com
PREFACE
The availability of lasers and the development of dynamic light scattering methods have led to a rebirth of interest in light scattering applications in polymer science and biophysical Chemistry. The spectral information in scattered light is now routinely used to measure diffusion coefficients, flow rates, and relaxation time distributions for complex systems.
Our original motivation for writing this brief text was to provide an introduction to modern light scattering methods that would encompass both classical methods and various forms of dynamic light scattering including photon correlation spectroscopy and electrophoretic light scattering with emphasis on biochemical applications. In the ensuing years instrumentation and data analysis methods have improved, but the basic concepts have not changed. Therefore, we are delighted that this book can now be made available to students and beginning research workers in a convenient format and at a modest price.
We hope that this text stimulates interest in scattering methods, and we have provided numerous references for additional reading. In particular we note the fine text by B. J. Bern and R. Pecora (Dynamic Light Scattering, Wiley, 1976). Also, we recommend the new introductory texts by Kenneth S. Schmitz (An Introduction to Scattering by Macromolecules, Academic Press, 1990) and Ben Chu (Laser Light Scattering: Basic Principles and Practice, Academic Press, 1991). For those requiring a comprehensive, up-to-date treatment of modern research applications we recommend Laser Light Scattering edited by Wyn Brown (Clarendon Press, 1993).
We thank CRC Press for permitting this text to be reprinted with minor corrections. Also, we thank David M. Johnson for preparing the original figures.
CHARLES S. JOHNSON, JR.
DON A. GABRIEL
Chapel Hill, North Carolina
May 1994
Table of Contents
DOVER BOOKS ON PHYSICS
Title Page
Copyright Page
PREFACE
I. INTRODUCTION
II. CLASSICAL LIGHT SCATTERING
III. DYNAMIC LIGHT SCATTERING
APPENDIXES
REFERENCES
I. INTRODUCTION
Light scattering has provided an important method for characterizing macromolecules for at least three decades. However, the replacement of conventional light sources by lasers in recent years has qualitatively changed the field and has sparked renewed interest. Through the use of intense, coherent laser light and efficient spectrum analyzers and autocorrelators, experiments in the frequency and time domains can now be used to study molecular motion, e.g., diffusion and flow, and other dynamic processes, as well as the equilibrium properties of solutions. The technology for clarifying samples has also significantly improved. Recirculation through submicron filters in closed loop systems reduces the effects of dust and other contaminants, and the new time domain techniques provide built-in tests for the presence of such particles. These advances make laser light scattering a powerful form of spectroscopy for use in biochemistry.
Classical light scattering studies are concerned with the measurement of the intensity of scattered light as a function of the scattering angle. In addition to this kind of study, laser light sources now permit spectral information to be obtained from the scattered light. The latter type of experiment is often called quasi-elastic light scattering (QLS), and the various forms of the experiment are known as light beating spectroscopy (LBS), intensity fluctuation spectroscopy (IFS), and photon correlation spectroscopy (PCS). Related experiments in laser doppler velocimetry (LDV) now permit very low rates of uniform motion to be measured. A special case of LDV is electrophoretic light scattering (ELS) where mobilities are determined. The aim of this book is to provide an introduction to both the classical and quasi-elastic forms of laser light scattering, which can serve as an introductory text for students and a reference for research workers. The same purpose has been served for prelaser classical light scattering since 1960 by Chapter 5 of Tanford’s excellent book, Physical Chemistry of Macromolecules.¹ As in that chapter, we emphasize concepts and the kinds of information that can be obtained from the various experiments rather than either presenting a comprehensive survey of the literature or discussing experimental techniques in great detail. For the most part we stick to well-developed techniques such as the measurement of translational and rotational diffusion coefficients. A few specialized applications (such as the study of motility) are also discussed; however, we have not included scattering from internal modes in polymers or gels. Interesting work is going on in the latter areas, but the theory is still in flux.
We attempt to keep the objectives in sight at all times and to avoid distracting complications as much as possible. Accordingly, certain definitions and derivations have been relegated to a set of appendixes. In general, we have opted for simplicity rather than elegance, or even rigor in certain cases, in the hope that a wider audience can be reached. For those who desire more extensive treatments there is an abundance of sources. For classical scattering, one of the latest reviews is that by Timasheff and Townsend, which is concerned with proteins.² Also Huglin³ has edited an extensive volume that emphasizes experimental methods and data handling in the study of polymer solutions. The book by Fabelinskii⁴ is a comprehensive, but difficult treatment mainly of prelaser light scattering without special attention to macromolecules. The classical physics of light scattering has been treated in detail by Kerker.⁵ The book by Long,⁶ while emphasizing Raman scattering, provides a good introduction to Rayleigh scattering, especially polarization and electronic resonance effects. Quasi-elastic scattering, also called dynamic light scattering, has received considerable attention in the literature, and the recent review papers are too numerous to list in full. An encyclopedic review of dynamic light scattering from biopolymers and biocolloids has been provided by Schurr.⁷ Pusey and Vaughan have given a much briefer review of the principles of intensity fluctuation spectroscopy,⁸ and Carlson⁹ has covered applications in molecular biology. Laser velocimetry has been reviewed by Ware.¹⁰.¹¹ An introduction to the theoretical foundations of dynamic light scattering is presented in the book by Berne and Pecora,¹² which is an excellent text for students of physical chemistry. The book by Chu¹³ is a good reference for many experimental and theoretical details. For those attempting to understand photon statistics and photon correlation spectroscopy, the monograph edited by Cummins and Pike is invaluable.¹⁴
II. CLASSICAL LIGHT SCATTERING