Formulation for Eagles Minimum Essential

Medium (EMEM) ATCC 30-2003

Inorganic Salts (g/liter)

CaCl2 (anhydrous)
MgSO4 (anhydrous)
KCl
NaHCO3
NaCl
NaH2PO4H2O

Vitamins (g/liter)
0.20000
0.09767
0.40000
1.50000
6.80000
0.14000

Amino Acids (g/liter)

L-Alanine
L-ArginineHCl
L-AsparagineH2O
L-Aspartic Acid
L-Cystine2HCl
L-Glutamic Acid
L-Glutamine
Glycine
L-HistidineHClH2O
L-Isoleucine
L-Leucine
L-LysineHCl
L-Methionine
L-Phenylalanine
L-Proline
L-Serine
L-Threonine
L-Tryptophan
L-Tyrosine2Na2H2O
L-Valine

0.00890
0.12640
0.01500
0.01330
0.03120
0.01470
0.29200
0.00750
0.04190
0.05250
0.05250
0.07250
0.01500
0.03250
0.01150
0.01050
0.04760
0.01000
0.05190
0.04680

Choline Chloride
Folic Acid
myo-Inositol
Nicotinamide
D-Pantothenic Acid
(hemicalcium)
PyridoxineHCl
Riboflavin
ThiamineHCl

0.00100
0.00100
0.00200
0.00100
0.00100
0.00100
0.00010
0.00100

Other (g/liter)
D-Glucose
Phenol Red, Sodium Salt
Sodium Pyruvate

ATCC (American Type Culture Collection)

P.O. Box 1549
Manassas, VA 20108 USA
www.atcc.org

1.00000
0.01000
0.11000

800-638-6597 or 703-365-2700
Fax: 703-365-2750
E-mail: tech@atcc.org
Or contact your local distributor
- Page 1 of 1 -