TRUE OR FALSE 1. Adsorption chromatography is not suitable for the separation of mixtures of polymers. 2.

Gel permeation chromatography would be the method of choice for the separation of non-polar industrial macromolecules. 3. In gas chromatography the mobile phase is always gas but the stationary phase should be solid to allow for better separation. 4. An open tubular column is that type of column that is open at both ends. 5. In planar chromatography the stationary phase can be ordinary paper impregnated with special substance. It could also be silica gel spread on aluminum foil 6. Rf in TLC means ratio of frontiers. 7. A supercritical fluid (SCF) is characterized by physical and thermal properties that are between those of the pure liquid and gas. 8. A supercritical fluid readily penetrates porous and fibrous solids because its diffusivity is higher than that of a liquid. 9. In partition chromatography, separation is based on partitioning of solutes between two immiscible liquids. Therefore we would expect it to occur only in liquid chromatography.

PROVIDE THE MISSING WORSD OR PHRASES 1. The parameter that controls the separation in LC is the polarity of the eluent, in GC it is the -----------------And in SFC it is the ------------------------------affinity for the

2. In adsorption chromatography the solute with the stationary stays ------------------- on the column.

3. In liquid chromatography separation is based on differences between the -----------------of the components in the ------------------------------- and the ----------------------------------

4. In Gas Liquid Chromatography separation is based mainly on differences between the ------------- of the sample components in the --------------------- stationary phase 5. Chromatography can be classified in three different ways. These are a) -------------------------------------b) --------------------------------------c) -----------------------------------------