E1

E2 E5

E4

E3

Counting of cells Cells from the E1, E2, E3, E4 & E5 squares were counted under a microscope. E1 + E2 + E3 + E4 + E5 = Number of cells counted The number of cells was calculated using the formula: Number of cells = Number of cells counted x 50,000 = X cells/ml

E1 E5 E4
Counting of cells

E2 E3

Cells from the E1, E2, E3, E4 & E5 squares were counted under a microscope. E1 + E2 + E3 + E4 + E5 = Number of cells counted The number of cells was calculated using the formula: Number of cells = Number of cells counted x 1000 = X cells/ml

Concentrations given for cell viability assay (MTT assay):

Separate treatment : Arecoline : 25, 50, 75, 100, 150, 200 g / mL Glutathione : 200, 400, 600, 800 g / mL Curcumin : 20, 40, 80, 120, 160 mol / L Combination treatment : Arecoline + Glutathione : (25, 50, 75, 100, 150) + 400 Arecoline + Curcumin : (25, 50, 75, 100, 150) + 20 Arecoline + Curcumin : (25, 50, 75, 100, 150) + 40 Treated for 24 hrs in 96 well plates (separate and combination).