Electrochemically Activated Solutions: Potential New Tools in the

Management of Diabetes and its Complications

Authors: N. L. Darnell, M. D. Trow

Document Info: Date: 9/21/2013, Version: 1.8, Number of Pages: 6, Security: Public, Contact: n.l.darnell@live.com

Copyright: This document is copyright protected. It may be referenced and linked. It may not be duplicated or
redistributed without permission from the author.

Abstract: Over the past two decades diabetes has evolved into a global epidemic that is currently threatening the
economies of all nations. The United States alone spends approximately $250 billion per year on diabetes management.
Nearly 25% of American adolescents are now classified as either diabetic or pre-diabetic. Disturbingly, standard medical
interventions intended to curb the disease have not proven effective in this age group. The New England Journal of
Medicine now predicts that the latest generation of American youth may be the first generation in U.S. history not to live
longer than the generation that preceded it due to complications associated with both obesity and diabetes. If we are
genuinely interested in suppressing the epidemic of diabetes then we must identify simple solutions that measurably reduce
risks associated with the development of the disease while simultaneously reducing health care costs and environmental
burdens. The routine consumption of electrolyzed reduced water has demonstrated increased insulin sensitivity, stabilized
serum glucose levels, reductions in LDL cholesterol, inflammation reduction, weight loss, and in some cases complete
reversal of metabolic syndrome. The topical applications of electrolyzed oxidizing water and electrolytic sodium hypochlorite
have demonstrated improved wound outcomes and a reduced need for antibiotics and surgical interventions among diabetic
patients. Electrochemically activated solutions are time tested and clinically proven tools that deserve serious consideration
as cost effective countermeasures against the growing epidemic of diabetes and its complications.

Key Words: diabetes, type 1, type 2, pre-diabetes, metabolic syndrome, hydrogen rich water, electrolyzed reduced water,
electrolyzed oxidizing water, electrolytic sodium hypochlorite, hypochlorous acid

Disclaimer: Statements contained in this document have not been evaluated by the Food and Drug Administration. They
are not intended to diagnose, treat, cure, or prevent any disease.

Introduction disease [21]. Fifteen years ago, the occurrence of

Over the past two decades diabetes has evolved into a
global epidemic of unprecedented magnitude. It now
accounts for 12% of all medical expenditures and the
costs are climbing [1]. The growing rate of diabetes is a
clear long-term threat to the economies of all nations.
Diabetes is a gateway disease [2-4]. It increases risks
for developing cardiovascular disease, osteoporosis,
rheumatoid arthritis, neurological disorders, cancer, and
sexual dysfunction in both men and women [2, 5-19]. It
also elevates risks for developing blindness, kidney
disease, and the occurrence of lower extremity
amputation. Diabetes and its complications currently
cost the United States $250 billion per year [20].
Over the past twenty years the greatest increase in the
diagnosis of diabetes in the United States has been
among individuals 65 years of age and older. However,
greater numbers of youth are now developing the
diabetes type 2 in adolescents was almost unheard of. It
is now estimated that 25% of adolescents are either
diabetic or pre-diabetic [22]. Disturbingly, standard
medical interventions have proven to be ineffective in
this younger age group [23]. According to the New
England Journal of Medicine, the latest generation of
American youth may be the first in United States history
not to live longer than the generation that preceded it
due to complications from obesity and diabetes [24].
In recent decades, standard approaches to diabetes
intervention have been statistically ineffective at
arresting the progression of the disease and its runaway
costs. If we fail to implement results-oriented action,
diabetes will bankrupt our nation within a generation. If
we are genuinely interested in suppressing the disease
then we must begin addressing it in different terms using
different tools. In pursuit of this, we must identify simple
solutions that measurably improve metabolic risk factors
while simultaneously reducing health care costs and
environmental burdens. Electrochemically activated
solutions are time tested and clinically proven tools that

1
deserve serious consideration in pursuit of these
endeavors.
Electrochemically Activated Solutions
The use of electrochemically activated water in the
management of diabetes is based on the medically
documented Nordaneau Phenomenon [25-26], and
associated research conducted on naturally reduced
Hita Tenryousui water [27]. Electrolyzed reduced water
is rich in dissolved hydrogen gas. Hydrogen gas is more
commonly referred to in scientific literature as molecular
hydrogen [28]. Molecular hydrogen is a powerful free
radical suppressant, which exhibits insulin-like activity on
glucose uptake [29-30].
The routine consumption of electrolyzed reduced water
has demonstrated reductions in risks associated with
both diabetes mellitus type 1 and 2. The consumption of
1 liter of hydrogen rich water per day has demonstrated
stabilized serum glucose levels, reductions in LDL
cholesterol, reductions in reactive oxygen species
(ROS), inflammation reduction, weight loss, and in some
cases complete reversal of metabolic syndrome [31-44].
Individuals suffering from diabetes have elevated risks
associated with free radical damage and poor
circulation. Under contemporary Western medical
protocols, diabetic patients are prescribed increasing
quantities of antidiabetic, antibiotic, and anti-
inflammatory medications in addition to pain killers. The
long-term consumption of these medications frequently
results in renal damage, kidney failure, and the need for
hemodialysis. Once dependent upon hemodialysis,
quality of life significantly diminishes and the risks of
amputation and morbidity significantly increase. The
routine consumption of hydrogen rich water has
demonstrated a renal protective effect upon individuals
not yet dependent on hemodialysis, and improved
outcomes for those individuals who are [45-50].
Diabetes-related ulceration is a significant problem for
those suffering from diabetes mellitus type 1 and 2.
Increased inflammation and reduced blood flow
suppress immune function and increase risks of infection
and amputation. The topical application of electrolytic
sodium hypochlorite has demonstrated improved wound
outcomes and a reduced need for antibiotics and
surgical interventions among diabetic patients [51-60].
A Brief History of Electrochemically
Activated Solutions
Electrochemically activated solutions, which include
electrolyzed reduced water, electrolyzed oxidizing water,
2
electrolytic sodium hydroxide, and electrolytic sodium
hypochlorite can be produced through the use of
medically certified electrolytic water generators.
Electrolytic water generators rely on tap water, saline,
and electricity to produce a variety of aqueous solutions
containing various concentrations of dissolved hydrogen,
oxygen, sodium, and free chlorine.
Electrolytic water generators were first utilized on WWI
hospital ships to produce electrolytic sodium
hypochlorite for surgical disinfection [61]. Following
WWII, Japanese physicians used the technology to
produce electrolyzed reduced water for the treatment of
intestinal abnormal fermentation, acid indigestion,
chronic diarrhea, chronic constipation, and dyspepsia
[62]. Over the past twenty years the therapeutic effects
of electrolyzed reduced water have been documented
against numerous diseases in both animal and human
models. Dissolved molecular hydrogen has
demonstrated antioxidant, anti-inflammatory, and anti-
allergic properties with no risk of cytotoxicity. In addition
to antidiabetic effects, current research also suggests
that molecular hydrogen suppresses the development of
mitochondrial myopathies, neurological disorders, and
arthritis. Molecular hydrogen is the only antioxidant that
is capable of immediately arresting intracellular and
intracerebral oxidative stress [63-66]. Electrolytic water
technology has been used by NASA for over sixty years
[67]. Surprisingly, the technology has been largely
omitted from Western health care.
Implementation of Electrochemically
Activated Solutions
Medically certified mixed-use electrolytic water
generators can produce electrolyzed reduced water for
consumption and electrolyzed oxidizing water,
electrolytic sodium hydroxide, and electrolytic sodium
hypochlorite for cleaning and disinfection. All of these
electrochemically activated solutions are non-toxic and
100% biodegradable.
Both the FDA and CDC recognize electrolytic sodium
hypochlorite, also known as electrochemically generated
hypochlorous acid, as an environmentally friendly hard
surface disinfectant [68-69]. Hypochlorous acid is the
same natural biocide that is produced by white blood
cells in the human body. Clinical studies have
demonstrated to EPA standards that electrolytic sodium
hypochlorite can disinfect against MRSA, E. Coli,
Staphylococcus, Salmonella, Klebsiella pneumonia, and
over 40 other common pathogens in less than 30 seconds
with no risk of antibiotic resistance [70].
Medically certified electrolytic water generators are
designed for clinical use and incorporate design features
necessary for safety, efficacy, reliability, and continuous
duty. They can be easily integrated into any residential,
small business, or commercial kitchen. They require tap
water, electricity, and saline for operation. Saline can be
produced at extremely low cost utilizing food-grade table
salt and municipal tap water. Electrolytic water technology
requires an initial upfront investment. However, the
technology offers significant long-term savings in terms of
reduced cleaning and disinfection supply costs, reductions
in hazardous chemical storage and disposal costs, and
improvements in workplace safety. Most medically
certified electrolytic water generators are designed for 10
to 15 years of continuous use if properly maintained.
Most units can be infinitely refurbished. Medically certified
electrolytic water generators are cost effective multi-use
devices that deserve a place in modern health
management.
and runaway costs. If we fail to implement results-
oriented action, diabetes will bankrupt our nation within a
generation. If we are genuinely interested in
suppressing the disease then we must begin addressing
it in different terms using different tools. In pursuit of
this, we must identify simple solutions that measurably
improve metabolic risk factors while simultaneously
reducing health care costs and environmental burdens.
Electrochemically activated solutions are time tested and
clinically proven tools that deserve serious consideration
in pursuit of these endeavors.
Acknowledgements
M. D. Trow is a lifelong educator and New Century
Fulbright Scholar. This document would not have been
feasible without her guidance and dedication.

Conclusion About the Author: N. L. Darnell is an executive

Diabetes is an epidemic of unprecedented magnitude.
Over the past twenty years standard Western medical
approaches to intervention have been statistically
ineffective at arresting the disease, its complications,
member of Diabetes Support Services Inc., a non-profit
community education and outreach organization
committed to improving the quality of life and longevity of
diabetics in the United States and around the world.
http://www.nldarnell.com

References:
1. Hu FB. Globalization of diabetes: the role
of diet, lifestyle, and genes. Diabetes
Care. 2011 Jun;34(6):1249-57. doi:
10.2337/dc11-0442.
http://www.ncbi.nlm.nih.gov/pubmed/
21617109
2. Kannel WB, McGee DL. Diabetes and
cardiovascular disease. The Framingham
study. JAMA. 1979 May
11;241(19):2035-8.
http://www.ncbi.nlm.nih.gov/pubmed/
430798
3. Bergman RN. New concepts in
extracellular signaling for insulin action:
the single gateway hypothesis. Recent
Prog Horm Res. 1997;52:359-85;
discussion 385-7.
http://www.ncbi.nlm.nih.gov/pubmed/
9238859
4. Kota SK, Meher LK, Jammula S, et al.
Aberrant angiogenesis: The gateway to
diabetic complications. Indian J
Endocrinol Metab. 2012 Nov;16(6):918-
30. doi: 10.4103/2230-8210.102992.
http://www.ncbi.nlm.nih.gov/pubmed/
23226636
5. Khoury JC, Kleindorfer D, Alwell K, et al.
Diabetes mellitus: a risk factor for
ischemic stroke in a large biracial
population. Stroke. 2013 Jun;44(6):1500-
4. doi: 10.1161/
STROKEAHA.113.001318. Epub 2013
Apr 25.
http://www.ncbi.nlm.nih.gov/pubmed/
23619130
6. Biessels GJ, Kappelle LJ; Utrecht
Diabetic Encephalopathy Study Group.
Increased risk of Alzheimer's disease in
Type II diabetes: insulin resistance of the
brain or insulin-induced amyloid
pathology? Biochem Soc Trans. 2005
Nov;33(Pt 5):1041-4.
http://www.ncbi.nlm.nih.gov/pubmed/
16246041
7. Xu WL, von Strauss E, Qiu CX, Winblad
B, Fratiglioni L.Uncontrolled diabetes
increases the risk of Alzheimer's disease:
a population-based cohort study.
Diabetologia. 2009 Jun;52(6):1031-9.
doi: 10.1007/s00125-009-1323-x. Epub
2009 Mar 12.
http://www.ncbi.nlm.nih.gov/pubmed/
19280172
8. Hu G, Jousilahti P, Bidel S, Antikainen R,
Tuomilehto J. Type 2 diabetes and the
risk of Parkinson's disease. Diabetes
Care. 2007 Apr;30(4):842-7. Epub 2007
Jan 24.
http://www.ncbi.nlm.nih.gov/pubmed/
17251276
9. Schernhammer E, Hansen J, Rugbjerg
K, Wermuth L, Ritz B. Diabetes and the
risk of developing Parkinson's disease in
Denmark. Diabetes Care. 2011
May;34(5):1102-8. doi: 10.2337/dc10-
1333. Epub 2011 Mar 16.
http://www.ncbi.nlm.nih.gov/pubmed/
21411503
10. Sun Y, Chang YH, Chen HF, Su YH, Su
HF, Li CY. Risk of Parkinson disease
onset in patients with diabetes: a 9-year
population-based cohort study with age
and sex stratifications. Diabetes Care.
2012 May;35(5):1047-9. doi: 10.2337/
dc11-1511. Epub 2012 Mar 19.
http://www.ncbi.nlm.nih.gov/pubmed/
22432112
11. Pandey A, Forte V, Abdallah M, Alickaj A,
Mahmud S, Asad S, McFarlane SI.
Diabetes mellitus and the risk of cancer.
Minerva Endocrinol. 2011 Sep;36(3):187-
209.
http://www.ncbi.nlm.nih.gov/pubmed/
22019750
12. Zhang PH, Chen ZW, Lv D, Xu YY, Gu
WL, Zhang XH, Le YL, Zhu HH, Zhu YM.
Increased risk of cancer in patients with
type 2 diabetes mellitus: a retrospective
cohort study in China. BMC Public
Health. 2012 Jul 28;12:567. doi:
10.1186/1471-2458-12-567.
http://www.ncbi.nlm.nih.gov/pubmed/
22839452
13. Hardefeldt PJ, Edirimanne S, Eslick GD.
Diabetes increases the risk of breast
cancer: a meta-analysis. Endocr Relat
Cancer. 2012 Nov 19;19(6):793-803. doi:
10.1530/ERC-12-0242. Print 2012 Dec.
http://www.ncbi.nlm.nih.gov/pubmed/
23035011

3
14. Hakim LS, Goldstein I. Diabetic sexual
dysfunction. Endocrinol Metab Clin North
Am. 1996 Jun;25(2):379-400.
http://www.ncbi.nlm.nih.gov/pubmed/
8799705
15. Enzlin P, Mathieu C, Van den Bruel A , et
al. Sexual dysfunction in women with
type 1 diabetes: a controlled study.
Diabetes Care. 2002 Apr;25(4):672-7.
http://www.ncbi.nlm.nih.gov/pubmed/
11919123
16. Morano S. Pathophysiology of diabetic
sexual dysfunction. J Endocrinol Invest.
2003;26(3 Suppl):65-9.
http://www.ncbi.nlm.nih.gov/pubmed/
12834025
17. Enzlin P, Mathieu C, Van Den Bruel A,
Vanderschueren D, Demyttenaere K.
Prevalence and predictors of sexual
dysfunction in patients with type 1
diabetes. Diabetes Care. 2003
Feb;26(2):409-14.
http://www.ncbi.nlm.nih.gov/pubmed/
12547871
18. Burke JP, Jacobson DJ, McGree ME, et
al. Diabetes and sexual dysfunction:
results from the Olmsted County study of
urinary symptoms and health status
among men. J Urol. 2007
Apr;177(4):1438-42.
http://www.ncbi.nlm.nih.gov/pubmed/
17382749
19. Miner M, Seftel AD, Nehra A, et al.
Prognostic utility of erectile dysfunction
for cardiovascular disease in younger
men and those with diabetes. Am Heart
J. 2012 Jul;164(1):21-8. doi: 10.1016/
j.ahj.2012.04.006. Epub 2012 Jun 7.
http://www.ncbi.nlm.nih.gov/pubmed/
22795278
20. The American Diabetes Association,
Diabetes Statistics Website, http://
www.diabetes.org/diabetes-basics/
diabetes-statistics . Accessed July 10th,
2013.
21. Cheng YJ, Imperatore G, Geiss LS,
Wang J, Saydah SH, Cowie CC, Gregg
EW. Secular Changes in the Age-
Specific Prevalence of Diabetes Among
U.S. Adults: 1988-2010. Diabetes Care.
2013 May 1.
http://www.ncbi.nlm.nih.gov/pubmed/
23637354
22. CNN Health, Nearly 1 in 4 U.S. Teens
Facing Diabetes, http://www.cnn.com/
2012/05/21/health/adolescents-facing-
diabetes . Accessed July 10th, 2013.
23. Zeitler P, Hirst K, Pyle L, Linder B,
Copeland K, Arslanian S, Cuttler L,
Nathan DM, Tollefsen S, Wilfley D,
Kaufman F. A clinical trial to maintain
glycemic control in youth with type 2
diabetes. N Engl J Med. 2012 Jun
14;366(24):2247-56. doi: 10.1056/
NEJMoa1109333. Epub 2012 Apr 29.
http://www.ncbi.nlm.nih.gov/pubmed/
22540912
24. Olshansky SJ, Passaro DJ, Hershow
RC, et al. A potential decline in life
expectancy in the United States in the
21st century. N Engl J Med. 2005 Mar
17;352(11):1138-45.
http://www.ncbi.nlm.nih.gov/pubmed/
15784668
25. Gadek Z, Hamasaki T, Shirahata S, The
Nordenau-Phenomenon—Facts and
Hypotheses. Animal Cell Technology:
Products from Cells, Cells as Products,
2000, pp 525-527
http://www.springerlink.com/content/
m77w47451p612670
26. Gadek Z, Hamasaki T, Shirahata S,
“Nordenau Phenomenon” – Application of
Natural Reduced Water to Therapy
Animal Cell Technology: Basic & Applied
Aspects Volume 15, 2009, pp 265-271
http://www.springerlink.com/content/
m77w47451p612670
27. Osada K, Li Y, Hamasaki T, et al, Anti-
Diabetes Effects of Hita Tenryou-Sui
Water ® , a Natural Reduced Water.
Animal Cell Technology: Basic & Applied
Aspects Volume 16, 2010, pp 307-313
http://link.springer.com/chapter/
10.1007/978-90-481-3892-0_51
28. Kikuchi K, Takeda H, Rabolt B, et al.
Hydrogen particles and supersaturation
in alkaline water from an Alkali–Ion–
Water electrolyzer Journal of
Electroanalytical Chemistry Volume 506,
Issue 1, 15 June 2001, pp 22–27
http://www.sciencedirect.com/science/
article/pii/S0022072801005174
29. Hanaoka K, Sun D, Lawrence R,
Kamitani Y, Fernandes G. The
mechanism of the enhanced antioxidant
effects against superoxide anion radicals
of reduced water produced by
electrolysis. Biophys Chem. 2004 Jan
1;107(1):71-82.
http://www.ncbi.nlm.nih.gov/pubmed/
14871602
30. Oda M, Kusumoto K, Teruya K, et al.
Electrolyzed and Natural Reduced Water
Exhibit Insulin-Like Activity on Glucose
Uptake into Muscle Cells and Adipocytes.
Animal Cell Technology: Products from
Cells, Cells as Products 2000, pp 425-
427
http://link.springer.com/content/pdf/
10.1007/0-306-46875-1_90
31. Shirahata S, Hamasaki T, Haramaki K, et
al. Anti-diabetes effect of water
containing hydrogen molecule and Pt
nanoparticles. BMC Proc. 2011 Nov 22;5
Suppl 8:P18. [Epub ahead of print]
http://www.ncbi.nlm.nih.gov/pubmed/
22373056
32. Kamimura N, Nishimaki K, Ohsawa I,
Ohta S. Molecular hydrogen improves
obesity and diabetes by inducing hepatic
FGF21 and stimulating energy
metabolism in db/db mice.
Obesity (Silver Spring). 2011
Jul;19(7):1396-403. doi: 10.1038/
oby.2011.6. Epub 2011 Feb 3.
http://www.ncbi.nlm.nih.gov/pubmed/
21293445
4
33. Li Y, Hamasaki T, Nakamichi N, et al.
Suppressive effects of electrolyzed
reduced water on alloxan-induced
apoptosis and type 1 diabetes mellitus.
Cytotechnology. 2011 Mar;63(2):119-31.
Epub 2010 Nov 10.
http://www.ncbi.nlm.nih.gov/pubmed/
21063772
34. Chen H, Sun YP, Li Y, et al. Hydrogen-
rich saline ameliorates the severity of l-
arginine-induced acute pancreatitis in
rats. Biochem Biophys Res Commun.
2010 Mar 5;393(2):308-13. Epub 2010
Feb 6.
http://www.ncbi.nlm.nih.gov/pubmed/
20138831
35. Kajiyama S, Hasegawa G, Asano M, et
al. Supplementation of hydrogen-rich
water improves lipid and glucose
metabolism in patients with type 2
diabetes or impaired glucose tolerance.
Nutr Res. 2008 Mar;28(3):137-43.
http://www.ncbi.nlm.nih.gov/pubmed/
19083400
36. Kim MJ, Jung KH, Uhm YK, Leem KH,
Kim HK. Preservative effect of
electrolyzed reduced water on pancreatic
beta-cell mass in diabetic db/db mice.
Biol Pharm Bull. 2007 Feb;30(2):234-6.
http://www.ncbi.nlm.nih.gov/pubmed/
17268057
37. Kim MJ, Kim HK. Anti-diabetic effects of
electrolyzed reduced water in
streptozotocin-induced and genetic
diabetic mice. Life Sci. 2006 Nov
10;79(24):2288-92. Epub 2006 Aug 2.
http://www.ncbi.nlm.nih.gov/pubmed/
16945392
38. Jin D, Ryu SH, Kim HW, et al. Anti-
diabetic effect of alkaline-reduced water
on OLETF rats. Biosci Biotechnol
Biochem. 2006 Jan;70(1):31-7.
http://www.ncbi.nlm.nih.gov/pubmed/
16428818
39. Li Y, Nishimura T, Teruya K, et al.
Cytotechnology. 2002 Nov;40(1-3):139-
49. Protective mechanism of reduced
water against alloxan-induced pancreatic
beta-cell damage: Scavenging effect
against reactive oxygen species.
http://www.ncbi.nlm.nih.gov/pubmed/
19003114
40. Hashimoto M, Katakura M, Nabika T, et
al. Effects of hydrogen-rich water on
abnormalities in a SHR.Cg-Leprcp/
NDmcr rat - a metabolic syndrome rat
model. Med Gas Res. 2011 Nov
3;1(1):26.
http://www.ncbi.nlm.nih.gov/pubmed/
22146083
41. Nakao A, Toyoda Y, Sharma P, Evans M,
Guthrie N. Effectiveness of hydrogen rich
water on antioxidant status of subjects
with potential metabolic syndrome-an
open label pilot study. J Clin Biochem
Nutr. 2010 Mar;46(2):140-9. Epub 2010
Feb 24.
http://www.ncbi.nlm.nih.gov/pubmed/
20216947
42. Murakami K, Sasaki S, Takahashi Y,
Uenishi K. Association between dietary
acid-base load and cardiometabolic risk
factors in young Japanese women. Br J
Nutr. 2008 Sep;100(3):642-51. Epub
2008
http://www.ncbi.nlm.nih.gov/pubmed/
18279559
43. Abol-Enein H, Gheith OA, Barakat N,
Nour E, Sharaf AE. Ionized alkaline
water: new strategy for management of
metabolic acidosis in experimental
animals. Ther Apher Dial. 2009
Jun;13(3):220-4.
http://www.ncbi.nlm.nih.gov/pubmed/
19527469
44. Li Y, Han C, Li Y, et al. Impact of extra
waters on immunosystem in mice. Wei
Sheng Yan Jiu. 2004 Jul;33(4):422-5.
http://www.ncbi.nlm.nih.gov/pubmed/
15461264
45. Matsushita T, Kusakabe Y, Kitamura A,
Okada S, Murase K. Protective effect of
hydrogen-rich water against gentamicin-
induced nephrotoxicity in rats using blood
oxygenation level-dependent MR
imaging. Magn Reson Med Sci.
2011;10(3):169-76.
http://www.ncbi.nlm.nih.gov/pubmed/
21959999
46. Huang KC, Hsu SP, Yang CC, et al.
Electrolysed-reduced water dialysate
improves T-cell damage in end-stage
renal disease patients with chronic
haemodialysis. Nephrol Dial Transplant.
2010 Aug;25(8):2730-7. Epub 2010 Feb
26.
http://www.ncbi.nlm.nih.gov/pubmed/
20190245
47. Cardinal JS, Zhan J, Wang Y, et al. Oral
hydrogen water prevents chronic allograft
nephropathy in rats. Kidney Int. 2010
Jan;77(2):101-9. Epub 2009 Nov 11.
http://www.ncbi.nlm.nih.gov/pubmed/
19907413
48. Huang KC, Yang CC, Hsu SP, et al.
Electrolyzed-reduced water reduced
hemodialysis-induced erythrocyte
impairment in end-stage renal disease
patients. Kidney Int. 2006 Jul;70(2):391-
8. Epub 2006 Jun 7.
http://www.ncbi.nlm.nih.gov/pubmed/
16760903
49. Luciak M. Antioxidants in the treatment of
patients with renal failure. Rocz Akad
Med Bialymst. 2004;49:157-61.
http://www.ncbi.nlm.nih.gov/pubmed/
15631333
50. Huang KC, Yang CC, Lee KT, Chien CT.
Reduced hemodialysis-induced oxidative
stress in end-stage renal disease
patients by electrolyzed reduced water.
Kidney Int. 2003 Aug;64(2):704-14.
http://www.ncbi.nlm.nih.gov/pubmed/
12846769
51. Thorn RM, Lee SW, Robinson GM,
Greenman J, Reynolds DM.
Electrochemically activated solutions:
evidence for antimicrobial efficacy and
applications in healthcare environments.
Eur J Clin Microbiol Infect Dis. 2012
May;31(5):641-53. Epub 2011 Aug 2.
http://www.ncbi.nlm.nih.gov/pubmed/
21809085
52. Lipsky BA, Hoey C. Topical antimicrobial
therapy for treating chronic wounds. Clin
Infect Dis. 2009 Nov 15;49(10):1541-9.
http://www.ncbi.nlm.nih.gov/pubmed/
19842981
53. Tata MD, Kwan KC, Abdul-Razak MR,
Paramalingam S, Yeen WC. Adjunctive
use of superoxidized solution in chest
wall necrotizing soft tissue infection. Ann
Thorac Surg. 2009 May;87(5):1613-4.
http://www.ncbi.nlm.nih.gov/pubmed/
19379926
54. Martínez-De Jesús FR, Ramos-De la
Medina A, Remes-Troche JM, et al.
Efficacy and safety of neutral pH
superoxidised solution in severe diabetic
foot infections. Int Wound J. 2007
Dec;4(4):353-62. Epub 2007 Oct 22.
http://www.ncbi.nlm.nih.gov/pubmed/
17953678
55. Hadi SF, Khaliq T, Bilal N, Sikandar I,
Saaiq M, Zubair M, Aurangzeb S.
Treating infected diabetic wounds with
superoxidized water as anti-septic
agent : a preliminary experience. J Coll
Physicians Surg Pak. 2007
Dec;17(12):740-3.
http://www.ncbi.nlm.nih.gov/pubmed/
18182139
56. Goretti C, Mazzurco S, Nobili LA, et al.
Clinical outcomes of wide postsurgical
lesions in the infected diabetic foot
managed with 2 different local treatment
regimes compared using a quasi-
experimental study design: a preliminary
communication. Int J Low Extrem
Wounds. 2007 Mar;6(1):22-7.
http://www.ncbi.nlm.nih.gov/pubmed/
17344198
57. Bongiovanni CM. Superoxidized Water
Improves Wound Care Outcomes in
Diabetic Patients. Diabetic Microvascular
Complications Today, 2006; May/June:
11 – 14.
58. Vnitr Lek. Infections and diabetic foot
syndrome in field practice. 2006
May;52(5):411-6.
http://www.ncbi.nlm.nih.gov/pubmed/
16771079
59. Horita Y, Miyazaki M, Noguchi M,
Tadokoro M, Taura K, Ozono Y, Kohno S.
Healing of Fournier's gangrene of the
scrotum in a haemodialysis patient after
conservative therapy alone. Nephrol Dial
Transplant. 2000 Mar;15(3):419-21.
http://www.ncbi.nlm.nih.gov/pubmed/
10692532
5
60. Tanaka H, Hirakata Y, Kaku M, et al.
Antimicrobial activity of superoxidized
water. J Hosp Infect. 1996 Sep;34(1):43-
9.
http://www.ncbi.nlm.nih.gov/pubmed/
8880549
61. Electrolytic Hypochlorite for Hospital
Ships. Br Med J. 1916 Mar
25;1(2882):455-6.
http://www.ncbi.nlm.nih.gov/pubmed/
20768067
62. Li Y, Nishimura T, Teruya K, et al.
Protective mechanism of reduced water
against alloxan-induced pancreatic beta-
cell damage: Scavenging effect against
reactive oxygen species. Cytotechnology.
2002 Nov;40(1-3):139-49.
http://www.ncbi.nlm.nih.gov/pubmed/
19003114
63. Ohta S. Recent progress toward
hydrogen medicine: potential of
molecular hydrogen for preventive and
therapeutic applications. Curr Pharm
Des. 2011;17(22):2241-52.
http://www.ncbi.nlm.nih.gov/pubmed/
21736547
64. Ito M, Ibi T, Sahashi K, Ichihara M, Ito M,
Ohno K. Open-label trial and
randomized, double-blind, placebo-
controlled, crossover trial of hydrogen-
enriched water for mitochondrial and
inflammatory myopathies. Med Gas Res.
2011 Oct 3;1(1):24. doi: 10.1186/2045-
9912-1-24.
http://www.ncbi.nlm.nih.gov/pubmed/
22146674
65. Yoritaka A, Takanashi M, Hirayama M,
Nakahara T, Ohta S, Hattori N. Pilot
study of H(2) therapy in Parkinson's
disease: A randomized double-blind
placebo-controlled trial. Mov Disord.
2013 Feb 11. doi: 10.1002/mds.25375.
http://www.ncbi.nlm.nih.gov/pubmed/
23400965
66. Ishibashi T, Sato B, Rikitake M, et al.
Consumption of water containing a high
concentration of molecular hydrogen
reduces oxidative stress and disease
activity in patients with rheumatoid
arthritis: an open-label pilot study. Med
Gas Res. 2012 Oct 2;2(1):27. doi:
10.1186/2045-9912-2-27.
http://www.ncbi.nlm.nih.gov/pubmed/
23031079
67. Darnell NL. Electrolyzed Water: Its
History and Relevance to Modern Health
Management. 2013. http://
www.scribd.com/doc/153843823
68. Centers for Disease Control and
Prevention, Guideline for Disinfection
and Sterilization in Healthcare Facilities,
2008. http://www.cdc.gov/hicpac/
disinfection_sterilization/
6_0disinfection.html. Accessed July
19th, 2013.
69. United States Department of Agriculture,
Peer Reviewed Articles of Antimicrobials
Approved by the FDA and FSIS as Safe
and Suitable Ingredients. http://
www.fsis.usda.gov/wps/wcm/connect/
234497ae-1f07-41ee-b7f2-
5c75d91162e0/
Chemical_+Antimicrobials.pdf?
MOD=AJPERES. Accessed July 19th,
2013.

70. Thorn R., Lee S., Robinson G.,

Greenman J., Reynolds D.,
Electrochemically activated solutions:
evidence for antimicrobial efficacy and
applications in healthcare environments,
European Journal of Clinical
Microbiology & Infectious Diseases,
Volume 31, Number 5 (2012), 641-653.
http://www.ncbi.nlm.nih.gov/pubmed/
21809085