Antibacterial Property of African Snail Hemolymph

B. subtilis
Replication
1
2
3
7.00
7.00
5.00
9.00
8.00
12.00
9.00
9.00
11.00
6.00
7.00
6.00
18.00
20.00
16.00

Treatment
25% Hemolymph
50% Hemolymph
100% Hemolymph
Water
Amoxicillin

Total

Mean

19.00
29.00
29.00
19.00
54.00

6.33
9.67
9.67
6.33
18.00

F
30.147
06

Pvalue
1.48E05

ANOVA
Source of
Variation
Between
Groups
Within Groups

SS
273.3333
333
22.66666
667

Total

df

296

4
10

MS
68.333
33
2.2666
67

14

Posthoc test:
Bonferroni-Holm
Group 1

Group 2

Water

Amoxicillin

25% Hemolymph

Amoxicillin

100% Hemolymph

Amoxicillin

50% Hemolymph

Amoxicillin

100% Hemolymph

Water

Critic
al
0.005
00
0.005
56
0.006
25
0.007
14
0.008
33

P
0.000
63
0.000
94
0.003
34
0.007
49
0.011
06

Significan
t?
Yes
Yes
Yes
No
No

F crit
3.478
05

E. coli
Treatment
25% Hemolymph
50% Hemolymph
100% Hemolymph
Water
Amoxicillin

Replication
1
2
3
9.00
10.00
10.00
14.00
10.00
12.00
8.00
11.00
10.00
8.00
9.00
10.00
15.00
18.00
16.00

Source of
Variation
Between
Groups
Within Groups

SS
109.3333
333
20

Total

129.3333
333

df
4
10

MS
27.333
33
2

Total

Mean

29.00
36.00
29.00
27.00
49.00

9.67
12.00
9.67
9.00
16.33

F
P-value
13.666 0.0004
67
62

14

Posthoc
test:
BonferroniHolm
Group 1
25%
Hemolymph
Water

Group 2
Amoxicillin
Amoxicillin

Critic
al
0.005
00
0.005

P
0.002
11
0.002

Significa
nt?
Yes
Yes

F crit
3.478
05

100%
Hemolymph
50%
Hemolymph

56
0.006
25
0.007
14

Amoxicillin
Amoxicillin

24
0.005
90
0.040
64

Yes
No

S. aureus

Treatment
25% Hemolymph
50% Hemolymph
100% Hemolymph
Water
Amoxicillin

Source of
Variation
Between
Groups

1
9.00
15.00
11.00
8.00
17.00

Within Groups

SS
158.2666
667
28.66666
667

Total

186.9333
333

Posthoc
test:

Replication
2
3
12.00
14.00
14.00
11.00
12.00
13.00
9.00
8.00
18.00
20.00

df
4
10

14

MS
39.566
67
2.8666
67

Total

Mean

35.00
40.00
36.00
25.00
55.00

11.67
13.33
12.00
8.33
18.33

F
P-value
13.802 0.0004
33
44

F crit
3.478
05

BonferroniHolm
Group 1

Group 2

Water
100%
Hemolymph
100%
Hemolymph
50%
Hemolymph
25%
Hemolymph

Amoxicillin
Amoxicillin
Water
Water
Amoxicillin

Critic
al
0.005
00
0.005
56
0.006
25
0.007
14
0.008
33

P
0.000
45
0.003
86
0.005
33
0.016
01
0.017
21

Significa
nt?
Yes
Yes
Yes
No
No