SAMPLE Matrix: hair Sample preparation: Wash hair in water, rinse 3 times with MeOH, dry, weigh.

5-25 mg Washed hair + 1 mL 1 M NaOH, heat at 70 !r 30 min, ad"#st $H t! %.5-10. 1 mL &'tra(t + 1 )'g $r!tri$ty*ine + 1 mL water + 1 mL 200 mM s!di#m (ar+!nate +# er, mi', e'tra(t with he'ane,+#tan!* %5,5 !r 20 min. -em!.e the !rgani( *ayer and add it t! 100 /0LL 0.21 !rth!$h!s$h!ri( a(id, mi' !r 20 min, in"e(t a 30 /0LL a*i2#!t ! the a2#e!#s *ayer. HPLC VARIABLES Guard column: 15 3 3.2 7 #-m Newg#ard -4-15 Column: 100 3 6.7 8$heri-5 -4-915 Mobile pha e: Me9N,+# er 60,70 :;# er was 1.2 L 100 mM $H 7.0 NaH24O6 + 30 mL diethy*amine.< !lo" rate: # In$ection %olume: &' (etector: )V 216 CHR*MA+*GRAM Internal tandard: $r!tri$ty*ine :6< *+HER S)BS+A,CES Extracted: amitri$ty*ine, (*!mi$ramine, desi$ramine, d!thie$in, ha*!$erid!*, imi$ramine, mianserin, n!rtri$ty*ine -E./*R(S may +e inter eren(es