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0.52 −
=
Where,
W=the weight, in g, of the added substance in 100ml of the final solution
a =the depression of the freezing point of water produced by the medicament already in the
solution (calculated by multiplying the value for b for the medicament by the strength of the
solution express as % w/v)
b=the depression of freezing of water produced by 1 per cent w/v of the added substance
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Freezing Point Depression Method
Example 1.1
Rx ∆Tf 1%
NaCl 900mg 0.576
Dextrose q.s (20.5g) 0.101
0.9% ⁄5 7
=
Solution; 500 100
( = 0.18% ⁄5
.
.
.
( ×
.)
= =
.
For 500ml;
500
= (4.12 ) ×
100
Example 1.2
Rx
Ephedrine HCl 1g
Chlorobutol 0.5g
NaCl (1.4g) q.s
Distilled water q.s ad 200ml
Solution;
2") 200 ;
200
= (0.7 ) ×
100
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Freezing Point Depression Method
EXercise 1.3
Prepare 500ml NaHCO3 (∆T.f 1%=0.38) so that when it is dilute with the same amount of water, it would
be isotonic.
Solution;
Rx ∆T.f 1%
NaHCO3 Xg(13.7) 0.38
Water qs ad 500ml
NaHCO3 Xg
Water qs ad 1000ml
0.52 − 0.52 − 0
= = = 1.37 ℎ9ℎ ! !"$"9
0.38
1.37 χ
=
100 1000
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Freezing Point Depression Method
2. NaCl EQUIVALENT METHOD (E)
• Based on the factor called the sodium chloride equivalent which can be used to convert a
specified concentration of medicament to the concentration of medicament to the
concentration of sodium chloride that will produce the same osmotic effect.
• Standard → 0.9 % w/v NaCl at isotonic
• Method to calculate % at isotonic, ∆Tf 1% =0.576 ~ NaCl
0.52 − 0.52 − 0
= = = 0.9 ") 0.9% ⁄5
0.576
(:)''; <"$ &'<)'!!" <)"	'& = !" #$" >, @A1 <. 927)
=
0.576 (2)''; <"$ &'<)'!!" <)"	'& = 01 "2 $ℎ' !' !$)'ℎ$ )
2
•Multiply this by the strenght of the medicament.
3
•substract the result from 0.9 per cent; the
difference is strenght of sodium chloride necessary
to adjust the solution to iso-osmoticity.
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aCl Equivalent Method
Example 2.1
Solution
.
°C
E 1% ascorbic acid=
.°C = 0.18
Example 2.2
Rx
NaCl 0.2%w/v
Dextrose q.s
Ft. isotonic solution 500ml
Solution;
∆Tf 1% NaCl = 0.576°C
∆Tf 1% dextrose = 0.101 °C
.
°C
For dextrose 1 % (E1%) =
.°C = 0.18
(0.7%)(1%)
χ= = 3.89% &'($)"!'
0.18%
For 500ml;
3.89 χ
=
100 500
χ = 19.45 g
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aCl Equivalent Method
Example 2.3
Rx E 1%
Ephedrine HC 1g 0.3
Chlorobutol 0.5g 0.24
NaCl q.s
Distilled water 200ml isotonic
Solution;
For ephedrine HCl D × 100E % × 0.3 = 0.15%
.
For chlorobutol D
Example 2.4
Solution;
1% KCl equivalent to 0.4% NaCl Convert amount of NaCl required to
χ% ← 0.69% NaCl (refer to example 3) amount of KCl required
0.69
χ= = 1.725% F1
0.4
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aCl Equivalent Method
Example 2.5
Calculate the percentage of sodium chloride required to render a 0.5 per cent solution of
potassium chloride iso-osmotic with blood plasma.
Solution
Sodium chloride equivalent of 0.5 per cent potassium chloride = 0.76
∴ Percentage of sodium chloride for adjustment
= 0.9 − (0.5 Χ 0.76) = 0.9 − 0.38 = 0.52
Example 2.6
Calculate the percentage of anhydrous dextrose required to render a 1 per cent solution
of ephedrine hydrochloride iso-osmotic with body fluid.
∴ Percentage of sodium chloride for adjustment
= 0.9 − (1 Χ 0.3) = 0.6
Equivalent percentage of anhydrous dextrose = 0.6/0.18 = 3.33
Example 2.7
Select a suitable substance for an eye lotion 0.5 per cent of silver nitrate and calculate the
percentage required to render the lotion iso-osmotic with lachrymal secretion.
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aCl Equivalent Method
3. WHITE VINCENT METHOD
Principle:
→This method involves the addition of water to medicament to obtain an isotonic solution. This
followed by the addition of isotonic buffer solution or preservatives isotonic solution to the required
volume.
V = W X E1% X 111.1
Where;
V= V (ml) of isotonic solution that could be obtain in wg of drug in water (the amount of water to added
to form isotonic solution)
111.1= constant that could be find from volume for 1% isotonic NaCl 0.9% NaCl → 1% NaCl
1 g NaCl → 111.1 ml
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White Vincent & Sprowl Method
Example 3.1
Rx E 1%
Ephedrine HCl 1g 0.3
Chlorobutol 0.5g 0.24
NaCl q.s
Distilled water 200ml isotonic
∴ Amount of NaCl required to adjust 153.34ml to isotonic; This solution is isotonic (C)
0.9 01
= × 153.34 !" #$" = 1.38 01
100 !" #$"
4. SPROWL METHOD
• Using white Vincent method but w is set to constant, 0.3
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White Vincent & Sprowl Method
5. MILIEQUIVALENT (mEq)
• Definition; the gram equivalent weight of an ion is the ionic weight (the sum of atomic
weights of the element in an ion) in gram divided by the valence of that ion.
• A mililequivalent is one thousandth part of the gram equivalent weight, the same figure
expressed in milligram. Table 5.1 makes this clear
" 'ℎ$
H) '*#5 '$ 'ℎ$ =
5 '9=
Example
1 Eq sodium → 23/1 = 23g
1Eq chloride → 35.5/1 =35.5
• The weight of a salt containing 1mEq of a particular ion is obtained by dividing the
molecular weight of the salt by the valency of that ion multiply by the number of such
ion in the molecules
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Miliequivalent (mEq)
Table 5.2 makes this clear
Weight of salt
M. of Valency of Number of containing 1mEq
Ion Salt used the salt the ion ion in salt of the ion
Example (monovalent)
1 Eq → 58.5/1= 58.5g
Example
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Miliequivalent (mEq)
Conversion Equations
J ×I
J ×I
1000
J ×I
10000
• The BPC includes table showing the weight s of salt that contain 1 mEq of specified ion;
Mg of salt containing 1
Ion Miliequivalent (mEq) mg Salt mEq of specified ion
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Miliequivalent (mEq)
Example 5.1
Calculate the quantities of salt required for the following electrolyte solution;
Therefore;
30 mEq of potassium ion is provided by 30 X 74.5 mg of potassium chloride which will
also supply 30 mEq of chloride ion.
10 mEq of phosphate ion is provided by 10 X 179 mg of sodium phosphate which will
also supply 10 mEq of sodium ion.
5 mEq of magnesium ion is provided by 5 X 101.5 mg of magnesium chloride which will
also supply 5 mEq of chloride ion.
There remains a deficiency of 10mEq of each sodium and chloride ions which cn be
provided by 10 X 58.5 mg of sodium chloride. The formula becomes;
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Miliequivalent (mEq)
mg g mEq
J ×I
J ×I K+ Na+ Mg2+ Cl- HPO4-
1000
55 55
The fact that cation and anion balance confirm that the formula has been worked out correctly
Example 5.2
From Table 20.6 and the appropriate conversion equation, the required percentages are—
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Miliequivalent (mEq)
Example 5.3
It is not unusual for students to miscalculate the amounts for this type of formula due to
failing to appreciate that mEq is a unit of weight and not an abbreviation for mEq per litre. This
leads to an incorrect halving of the final quantities in the above example.
Example 5.4
Express 0.9 per cent sodium chloride solution to mililequivalent per litre.
0.9 × 10000
= 154 I*/ $)'
58.5
1 × 10000
= I*/ $')
J
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Miliequivalent (mEq)
Adjustment to Iso-osmoticity with Blood Plasma Based on Miliequivalent
Where;
a = number of mililequivalent per liter of medicament present and
b = number of mililequivalent per liter of adjusting substance required
Example 5.5
A solution containing 40 mEq of chloride ion provide a total of 80 mEq of anion and
cation.
∴ = 310 − 80
= 230
230 mEq will be provided by 115 mEq of sodium ions and 115 mEq chloride ions.
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Miliequivalent (mEq)
6. MILIMOLES
In the SI, the unit for chemical quantity is mole and the term equivalent and
mililequivalent becomes absolute. Consequently the method of expressing the composition of
body and infusion fluid is changing from the mililequivalent to mole notation.
By analogy with atoms and molecules, a mole of an ion is its ionic weight in grams but
the number of moles of each of the ions of a salt in solution depends on the number of each ion
in the molecule of the salt.
It follows that the quantity of salt, in mg, containing 1 mmol of a particular ion can be
found by dividing the molecular weight of the salt by the number of that ion contained in the
salt. For example—
Conversion Equations
To convert quantities expressed in mmol per litre into weighable amounts. The
following formulae may be used:-
<') $)' = J × M
Where W is the number of mg of salt containing 1 mmol of the required ion and M is the
number of mmol per litre
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Milimole(mmol)
Example 6.1
Calculate the quantities of salts required for the following electrolyte solution.
Sodium 60 mmol
Potassium 5 mmol
Magnesium 4 mmol
Calcium 4mmol
Chloride 81 mmol
Water for injection, to 1 litre
mmol
Although there appears to be inequality between the anions and cations, the charges are equally
balanced.
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Milimole(mmol)
Example 6.2
Calculate the number of millimoles of (a) dextrose and (b) sodium ions in 1 litre of
Sodium Chloride and Dextrose Injection containing 4.3 per cent w/v of anhydrous dextrose and
0.18 per cent w/v of sodium chloride.
Use the conversion equation—
a) For dextrose
Hence,
4.3 × 10000
M= = 239 "
180.2
Example 6.3
Calculate the number of milimoles of calcium and chloride ion in a litre of a 0.029 per
cent solution of calcium chloride.
0,029 × 10000
M= = 2 "
147
But, each mole of calcium chloride provides 1 mol of calcium ions and 2 moles of chloride ions.
Therefore, 1 litre of solution contains 2mmol of calcium ion and 4 mmol of chloride ion.
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Milimole(mmol)
ppm calculation
EXample
Prepare 90ml NaF solution so that 5ml of the solution when diluted with water to 1cupfull(240ml), a
3ppm solution obtained.
Solution;
(240 )(90 )
= = 4329
5
3g Na → 1000000ml
? ml ←4320ml
(OG
PQ)(GPQ)
= = 0.01296
PQ
Formula
NaF 13mg
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Calculation involving density factor
EXample
Given acetic acid BPC (33%w/w, d=1.04g/ml).What is the concentration of acetic acid in %w/w
Solution;
Acetic acid
d= 1.04g/ml
? w/v
(
PQ)(
R)
=
OR
= 96.15 9& !" #$"
33
= × 100 = 34% ⁄5
96.15
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