Definition of cytopathology

Cytopathology is the study of normal and abnormal

exfoliated cells in tissue fluid.

The individual cells reflect the normal and abnormal

morphology of the tissue from which they are derived.

Types of exfoliated cyto-pathology

Natural spontaneous exfoliation
Natural covering epithelium: skin, urinary tract, vagina, and

cervix.
Glandular epithelial secretion: Breast (Nipple secretion).

Sputum
Urine Exudates and transudate:

Pleural fluid Pericardial fluid

Peritoneal fluid Joint fluid CSF

Artificial enhanced exfoliation:

Scrapings from cervix, vagina, oral cavity, and skin

Brushing and lavage: bronchi, GIT, and urinary tract

Fine needle aspiration (FNA) for:

Body cavity fluid: pleural, pericardial & peritoneal fluids

Cysts: neck, breast & ovary Solid tissue: body organs, tumors & other swell

Role of cytopathology
Early detection of unsuspected diseases (malignant

or pre-malignant lesions).
Confirmation of suspected diseases without surgical

trauma.
Diagnosis of hormonal imbalance.

Useful in flow up the course of disease or monitoring

therapy.

Advantage of Cytopathology
Rapid diagnosis

- Inexpensive

- Simple

It is better in evaluating the infectious diseases.

Supplement or replace frozen section or biopsy

No injury to tissue allowing repeated sampling

It is better for hormonal assay

Cytopathological smear cover a wider surface than

that involved in surgical biopsy.

Disadvantage of Cytopathology
Interpretation of the morphological cellular changes is

based only on individual cell observation.

Not always finally diagnosis, so it is confirmed by

histopathology in some cases.

Not determine the size and type of lesion of some cases.

Factors that determine the appearance of cells

Type of the technique used. Level of cell maturation at the time of cell collection. Nature of the parents tissue: soft tissue, cyst, or solid organ.

Medium of the exfoliated cells.

Interval between the stain of the exfoliated cells and collection

of samples.
Type of fixative, stain, and processing of the technique

used.

PAP smear: named after Dr. George Papanicolaou (1883-1962)

Vaginal smears from guinea pigs (1917)
Women (1920)

Hormonal cycles Pathological conditions (1928)

Normal Cervix

Taking the Sample

Liquid Based Cytology lab processing

The Pap Smear

Cytologic screening for cervical cancer

Cervical cancer screening has decreased morbidity and

mortality
Deaths from cervical cancer decreased from 26,000 to

less than 5,000 between 1941 and 1997

Pap smears are not perfect

For a high grade lesion, the sensitivity of a single pap

smear is only 60-80%

Estimated false negative rate is 30-50% Requires adequate specimen collection

Requires adequate cytological review

 Requires adequate patient and physician follow-up

10% of women with cervical cancer had inappropriate

follow-up.
Requires access to care
50% of women with cervical cancer were never

screened and 10% had not been screened within 5 years

of diagnosis.

Who to screen
Any woman with a cervix who has ever had sexual

activity.

When to screen
Start within 3 years of onset of sexual activity or by age

of 21, whichever is first.

Risk factors for cervical dysplasia
Early onset of sexual activity
Multiple sexual partners

Tobacco
Oral contraceptives

Screening frequency
Yearly until three consecutive normal pap smears, then

may decrease frequency to every three years

Annual screening for high-risk women is highly

recommend.

When to stop routine screening

Age 65 and adequate recent screening
Three consecutive normal pap smears No abnormal pap smears in last 10 years No history of cervical or uterine cancer

Hysterectomy for benign disease Hysterectomy for invasive cervical cancer

Cervical histology

Original Squamous Epithelium

Vagina and outer ectocervix
4 cell layers Well-glycogenated (pink) unless atrophic

Columnar Epithelium
Upper and middle endo-cervical canal
Single layer of columnar cells arranged in folds Mucin producing (not true glands)

Squamous Metaplasia
Central ectocervix and proximal endocervical canal Replacement of columnar cells by squamous epithelium

Progressive and stimulated by

Acidic environment with onset of puberty Estrogen causing eversion of endocervix

Transformation Zone
Zone between original squamo-columnar junction and

the new squamo-columnar junction

Nabothian cysts visually identify the transformation

zone if present

Original Squamo-columnar Junction

Placement determined between 18-20 weeks gestation Most often found on ectocervix

Can be found in vagina or vaginal fornices

Less apparent over time with maturation of epithelium

New Squamo-columnar Junction

Border between squamous epithelium and columnar

epithelium
Found on ecto-cervix or in endo-cervical canal Majority of cervical cancers and precursor lesions

arise in immature squamous metaplasia, i.e. the leading edge of the squamo-columnar junction

Squamous Epithelium

Parabasal Cells

Intermediate Cells

Superficial

Cells

Endocervix

Endocervical Cells

Endometrial Cells

Non-Epithelial Cells

Lymphocytes

Polymorphs

sperms

Normal smear

Ectropion / Erosion
At puberty & pregnancy the endocervical cells are

pushed out to lie on the ectocervix

Normal

Ectropion

Wide Ectropion

Metaplasia
The endocervical cells are transformed into squamous

cells through the process of squamous metaplasia

Metaplastic Cells