A Review of Salmon Alphavirus (SAV 1-6) and Potential Vaccinations
in Atlantic Salmon (Salmo salar)
Biological Science, California State University Fullerton, 800 N State College Blvd, Fullerton, CA, 92831, US
Abstract Pancreas disease (PD) in Atlantic salmon (S. salar) has been a major factor contributing to salmon mortality, causing considerable economic losses to European fisheries and aquaculture businesses. The cause of PD went undiscovered when first described in 1987, since then its been attributed to salmon alphavirus (SAV) infection. Currently, SAV has been categorized into 6 subtypes based on genetic differences in RNA sequencing. The subtypes differ in their pathological intensities and in their geographical distributions. Upon SAV infection, S.salar experience intense heart and pancreas tissue damage. However, increased interferon and Mx protein load in conjunction with changes in blood serum protein abundance were associated with a fast-acting immune responses. Vaccines based on inactivated whole SAV strains have been developed to combat the spread of SAV and protect vital salmon stock from mortality. In this review article, we will compare SAV subtypes 1 and 3, possible vaccinations, and their effects on S. salar.
Introduction Since first described in 1987, Pancreas disease (PD) has been associated with total necrosis of exocrine pancreas, loss of feeding response and emaciation [1]. The occurrence of PD in S. salar greatly contributes to economic losses in numerous European fishing industries and aquaculture businesses. From January 2009 to December 2010, Southern Norway farming S.salar had reported highest PD outbreaks [2]. The outbreak reports were also associated with high mortality rates, interrupted reproduction cycles, and decreased growth rates [2]. Research efforts had not pinpointed the diseases causative agent, but closer examination of the pathology of PD may have been required to do so. Although research communities have known the consequences of PD for some time, its origin has only recently been discovered. The cause of PD in S. salar has been attributed to the salmon alphavirus (SAV) [3,4]. As a result, various research communities have increased efforts to scrutinize SAV and its effects on S. salar in order to find methods to alleviate economic losses. Increased knowledge of SAV spurred efforts to design vaccinations based on genomic strains of SAV subtypes and test them. In this review, we aim to determine the importance of the different SAV subtypes (1-6) and how subtypes are differentiated. We also explore research carried out to produce viable treatments and preventative methods. Determining the effects of SAV on S. salar and the success of potential vaccinations may benefit salmon farming industries in the future. SAV Subtypes The SAV subtypes have been described and categorized by number (1-6), based on genetic differences in their respective RNA sequences [5]. Fringuellis study utilized real time Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR) in order to track the number of genetic differences between subtypes. Sequencing analysis produced data on genetic differences and the subtypes were categorized based on percent similarity [5]. Pathological differences between subtypes were explored once the subtypes were categorized. Pathological inflictions vary between subtypes. Grahams study found that viral subtypes are spread via horizontal disease transmission; which is transmission between species members and not in a parent- offspring relationship [6]. Grahams study also showed that subtypes 1 and 3 cause higher intensities of pathological effects, including tissue damage to the heart and pancreas (Table 1). As a result, much of the research done on the effects of SAV and SAV vaccinations have tested SAV subtypes 1 and 3. Histology and qRT-PCR were used to determine differences in pathological effects, however, histology was claimed to be the better method for determination [6].
Graham et al. 2011
SAV subtypes were categorized based on their geographic distribution in Ireland and Scotland [7]. RT-PCR data showed a mixture of subtypes across the regions studied while SAV 1 was most abundant. Graham and Fringuelli [7] did not include SAV 3 as it occurs predominantly off the coast of Norway. SAV subtypes occur simultaneously across geographic regions with the exception of SAV 3, which is thought to occur only in Norway. The subtypes differ genetically and in the pathological intensity subsequent to infection. These findings are valuable for future studies that will explore vaccinations, diagnosis, and the epidemiology of SAV. Effects of SAV on S. salar Infection of SAV in S. salar is detrimental to their growth and health. Young S. salar experimentally infected with SAV-1 experienced pathological changes to the heart and pancreas which resulted in damaged tissue as early as three days after infection [8]. These changes were examined via immunohistopathology, which allowed researchers to provide a score for the amount of tissue damage, onto a tissue damage interval scaled [8]. SAV 1 infection resulted in more tissue damage to the pancreas than the heart, which is expected since SAV is a causative agent of PD in S. salar [8]. SAV 1 infection was also associated with a fast-acting immune response measured via interferon load and production of Mx proteins [8]. Interestingly, salmon mortality was not observed in this study. However, mortality rates may be associated with season changes [9]. The connection between S. salars potential sensitivity to environmental changes and SAV susceptibility has not been explored. Changes in protein abundance upon infection of SAV 3 were associated with variable pathological responses in S. salar [10]. The highest levels tissue damage were again observed in the pancreas, along with lower levels in the heart, and in red and white muscles [10]. The absence of recorded tissue damage in the red and white muscle may be attributed to differences in pathological responses between SAV subtypes [8]. Upon alteration of S. salars expressed blood serum proteins, protein abundance was associated with two phenomena: increased pancreas and heart damage or intensified early immune responses to infection [10]. S. salar are negatively affected by SAV in that the virus results in tissue damage and growth rate reduction. Better understanding of the immune responses and proteome modifications S. salar undergo post-infection will provide applicable treatments of SAV. Vaccines Since the association between SAV and PD was made, efforts have increased in order to produce viable vaccinations to combat SAV transmittance. Inactivated whole virus (IWV) vaccines were shown to provide better protection against SAV than DNA and subunit vaccines [11]. S. salar vaccinated with IWV experienced complete protection against mortality, while virus replication and pathological changes were inhibited in the pancreas and heart [11]. Similarly, IWVs were used to demonstrate effective protection against SAV infection and resulting PD [12]. This study was the first to explore the efficacy of vaccinations both in laboratory and field conditions. The consensus is that vaccinations based on inactivated SAV strains provide the most superior protection against infection, which prevents the development of PD. Implications Proteins such as interferon proteins, Mx proteins, and changes in protein abundance possess the ability for acting as biomarkers to detect SAV infection [1,6]. The examination of early immune responses by measuring changes in protein levels (interferon proteins or Mx proteins) may also be developed to utilize an artificial selection regime. The regime would consist of selecting individuals that possess the highest interferon and Mx protein production levels [8]. Those individuals could then be bred to pass on genetic advantages and so forth. Prior research would be necessary to develop safe and ethical methods for carrying out artificial selection of S. salar. SAV has been described in detail and research communities continue to seek out solutions to deter its spread and its effects on S. salar populations. Safe and effective vaccinations based on inactive SAV strains provide the best, most available option for inhibiting viral replication and protection from mortality caused by PD. References 1. McVicar, A. H. (1987). Pancreas disease of farmed Atlantic salmon, Salmo salar, in Scotland: Epidemiology and early pathology. Aquaculture, 67(1), 71-78.McVicar 2. Tavornpanich, S., Paul, M., Viljugrein, H., Abrial, D., Jimenez, D., & Brun, E. (2012). Risk map and spatial determinants of pancreas disease in the marine phase of Norwegian Atlantic salmon farming sites. BMC veterinary research, 8(1), 172. 3. Graham, D. A., Wilson, C., Jewhurst, H., & Rowley, H. (2008). Cultural characteristics of salmonid alphavirusesinfluence of cell line and temperature. Journal of fish diseases, 31(11), 859-868. 4. Weston, J. H., Graham, D. A., Branson, E., Rowley, H. M., Walker, I. W., Jewhurst, V. A., & Todd, D. (2005). Nucleotide sequence variation in salmonid alphaviruses from outbreaks of salmon pancreas disease and sleeping disease. Diseases of aquatic organisms, 66(2), 105- 111. 5. Fringuelli, E., Rowley, H. M., Wilson, J. C., Hunter, R., Rodger, H., & Graham, D. A. (2008). Phylogenetic analyses and molecular epidemiology of European salmonid alphaviruses (SAV) based on partial E2 and nsP3 gene nucleotide sequences. Journal of fish diseases, 31(11), 811-823. 6. Graham, D. A., Frost, P., McLaughlin, K., Rowley, H. M., Gabestad, I., Gordon, A., & McLoughlin, M. F. (2011). A comparative study of marine salmonid alphavirus subtypes 16 using an experimental cohabitation challenge model. Journal of fish diseases, 34(4), 273- 286. 7. Graham, D. A., Fringuelli, E., Rowley, H. M., Cockerill, D., Cox, D. I., Turnbull, T., ... & Mc Loughlin, M. F. (2012). Geographical distribution of salmonid alphavirus subtypes in marine farmed Atlantic salmon, Salmo salar L., in Scotland and Ireland. Journal of fish diseases, 35(10), 755-765. 8. Herath, T. K., Thompson, K. D., Adams, A., & Richards, R. H. (2013). Interferon-mediated host response in experimentally induced salmonid alphavirus 1 infection in Atlantic salmon (Salmo salar L.). Veterinary immunology and immunopathology, 155(1), 9-20. 9. Stormoen, M., Kristoffersen, A. B., & Jansen, P. A. (2013). Mortality related to pancreas disease in Norwegian farmed salmonid fish, Salmo salar L. and Oncorhynchus mykiss (Walbaum). Journal of fish diseases, 36(7), 639-645. 10. Braceland, M., Bickerdike, R., Tinsley, J., Cockerill, D., Mcloughlin, M. F., Graham, D. A., & Eckersall, P. D. (2013). The serum proteome of Atlantic salmon, Salmo salar, during pancreas disease (PD) following infection with salmonid alphavirus subtype 3 (SAV3). Journal of proteomics, 94, 423-436. 11. Xu, C., Mutoloki, S., & Evensen, . (2012). Superior protection conferred by inactivated whole virus vaccine over subunit and DNA vaccines against salmonid alphavirus infection in Atlantic salmon (Salmo salar L.). Vaccine, 30(26), 3918-3928. 12. Karlsen, M., Tingb, T., Solbakk, I. T., Evensen, ., Furevik, A., & Aas-Eng, A. (2012). Efficacy and safety of an inactivated vaccine against Salmonid alphavirus (family Togaviridae). Vaccine, 30(38), 5688-5694.