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Artificial cerebral spinal fluid (aCSF)

We use this for microdialysis in the brain and in some of our microfluidic
chips. If we were ex vivo and using enzymes we would add buffer salts.
- 147 mM NaCl
- 2.7 mM KCl
- 1.2 mM CaCl

- 0.85 mM MgCl

-
in distilled water and stirring till a colourless solution is formed.
!ou can filter it "membrane below #. $m even ma%es it sterile& in fact we filter
to #&#um starting with a #.' $m membrane& but this is because we have
other membranes in the system.(
Can add Kathon "'##$)*) of solution enough( to avoid bacteria proliferation if
%eep the bottle for a long time. +therwise either ma%e freshly or freeze
)eave solution in the fridge.
,o add realistic protein content for interference studies use '- by weight
.ovine /erum 0lbumin "./0(.