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THE POLYMERASE
CHAIN REACTION
(PCR)
P50-54
Objective
Sensitive
Convenient
automatic
efficient
Disadvantage
Contamination
If the sample that is being tested has even
the smallest contamination with DNA from
the target, the reaction could amplify this
DNA and report a falsely positive
identification.
The reaction is limited in the size of the
DNAs to be amplified.
Application
Gene diagnose
Gene cloning
Gene screening