Micro Course

2013
TRAINING REPORT
Bio390

1433-1434

3 ..................................................................................
4 ........................................................
5 ...............................................................:
5 ....................................................................... :
5 ........................................................................ :
)6 ............................................... (Microbiology
: 6 .................................. : Specimens receiving
7 ................................................ :
: 9 ........................................ specimens culture
9 ............................................ :Culture Media
) (15 )Swabs&Bodyfluid specimens
) (16 ...................................... )Urine Specimens
) (17 .......... )Stool & Sputum Specimens
11 ........................................
: 19 .........................................................
Antibiotics
02 .............................................................. susciptbility test
02 .................................................................... :Vitek
04 ................................................................:Phoenix
)07 ............................................. :Manual (AST
01 .............................................. Antibiotics
) )09 ........................................ (SBI

45 ................................................................... :
) (51 ..........................................)Urine Specimen
1
62 ................................ :
) (60 .............. )Stool&Sputum Specimens
64 ............................... :
(67 .................................. )Blood Culture Specimens
71 ...................................................................Flow charts
73 ................................................................................
74 ..................................................................
79 ..........................................................................

, ((
)).

.

.

.
.

-1
.
-0 .
-3 Lab Coat .
-4 yellow Gown .
-5 Gloves .
-6 .Mask
-7 Bench .
-1 .
-9 .Safety Cabinet
Specimens
-12
Loops Swabs .

-11
( ) .
:
0011

.
.
, , ,
.
:

..
:
-1 Phlebotomy
-0 Reception
-3 Microbiology
Parasitology Tuberculosis -4 Molecular Virology
-5 Immunology & Serology
-6 Biochemistry
-7 Hematology
-1 Histology
-9 NAT
Blood Bank
-12
)(Microbiology

.
:
Culture Bench
)Swab & Body fluid bench (SB
)Stool & Sputum Bench (SS
Urine Bench
Blood Bench
: : Specimens receiving

.
:
-1 ()Blood Culture Specimens
()1-1
-0 ()Urine Specimens
()1-2
-3 ()Stool Specimens
()1-3
)Swab Specimens( -4
)Wound Swabs(
)Vaginal Swabs(
)Eye Swabs(
)Ear Swabs(
)Throat Swabs(
)Respiratory Swabs(
)Genital Swabs(
)Body Fluids Swabs(
pus -5
sputum -6
Cerebrospinal fluid (CSF) -7
: sterile body fluid -1
Synovial fluid Peritoneal fluid Precarditis and myocarditis fluid Pleural fluid -
)1-4(
: specimens culture
:Culture Media

.
)BAP( Blood Agar

Haemolysis
.Streptococcus
()2-1
()Chocolate Agar
X & hematin
, hymen V Nicotineamide Adenine Dinucleotide
) (NAD ) (RBCs
Haemophilus
()2-2
(MAC) Macconkey Agar

( )Selective Media
,(GNB) Gram Negative bacteria
, (GPB) Gram Positive Bacteria
( )Differential media
( ,)Lactose fermentation
( )positive (.)negative
()2-3
Sabouraud Dextrose Agar

Yeast, fungus
()2-4
12
XLD Agar
Salmonella, Shigella Differential Media
)2-5(
Selenite Broth Media

Salmonella ) Enriched Media(
)2-6(
11
)THIO( Thioglycollate broth

( )Enriched Media
, ( )Anaerobic
( )aerobic, anaerobic, microaerophilic
( )Obligate ()facultative
()2-7
Mannitol Salt Agar

Staphylococcus aureus
Mannitol
MRSA

(Methicillin Resistant Staph.aureus )MRSA
()2-8
10
Moueller hinton Agar

.
()2-9
)TM( Thayer Martin media

Nisseria
.
()2-10
: Bile Esculin Agar (BEA)

Streptococcus Enterococcus
.
()4-14
13
Todd Hewitt Broth media

Streptococci Group B
()2-11
Campy agar
Campylobacter Selective
media ( )2-12 Anaerobic jar Co2
( )2-13 Co2
()2-14
()2-13
()2-12
()2-14
14
) ( Swabs&Bodyfluid
)specimens
:

. ()2-15
.Fluid
Streaking .Loop ()2-16
04 37.
()2-16
()2-15
( )Pus swab B.A, MAC, CBA

Fluid THIO Swab
.
Centrifuge .
swab
.Loop
15
) ()Urine Specimens
:Urine
( )B.A/CLED ()2-17
Loop ( )2-18 .
Streaking (.)2-19
37 04
()2-17
()2-18
16
()2-19
) ()Stool & Sputum Specimens

:
XLD Agar , Selenite Broth
Swab XLD
swab Streaking
Loop ()2-20
Swab selenite broth .
37 04.
Sputum
:
B.A , CAB , MAC Slide Pus cells,

epithelial cells.
()2-20
17

Specimen
Bile
Cervical
CSF
Ear
Eye
Fluids:
BA
(O2)
+
+
+
+
+
Ascitic-CAPD-Drain fluid
Peritoneal-pericardial
BA
(AN
O2)
+
CBA
+
+
SDA
+
+
+
+
+
MAC
or
EMB
+
+
+
+
+
MSA
THIO
CLED
XLD
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
SELENITE BROTH
GS
+
+
+
+
+
Pleural-Synovial-Cyst Fluid
Fungal Culture
Mouth
Nasal-MRSA Screaning
Prostatic discharge
Pus
Semen
Sinus Aspirate
Skin Swab
Throat
Catheter Tip:
A (rolling)
Arterial line CVP- Femoral- IV Line
Jugular- Subclavian- Swans Gang- ventricular
B- Drain Tip:
Haemovac- Nephrostomy- Postoperative
Tissues
Umbilical Swab
Urethral Swab
Vaginal Swab
Wound Swab
Blood
Bone Marrow
Sputum
Brounchoalveolar Lavage (BAL)
Endotracheal Tube Suction Tip (ETT Suction Tip)
Gastric Lavage
Tracheostomy Secretion
Tracheostomy Tube Tip
Rectal Swab
Stool
Urine
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
11
G ve MAC G +ve / B.A

B.A.

( )
THIO Sub ( Fluid
. )culture
: Work Sheet
).Heavy Growth of (GNB) or (GPC
).Moderate Growth of (GNB) or (GPC
.No Growth
3 .Mix Growth.
Catheter tip:
)Significant/ No Sig. Growth of (GNB) or (GPC
12
No Significant Growth.
) (AST
) (ID Genus
.
)AST( .Antimicrobial Susceptibly Testing
)ID( .Bacterial identification
19

Antibiotics susceptibility test
:Vitek
Biochemical tests 64

Gram Positive, Gram Negative, Anaerobic, Yeast
Susceptibility Test .
()3-1
:
Suspension 3. Swab 0-1 . Nephelometer 0.50-0.60 (. )3-2
()3-2
02
rack Vitek (.)3-3 (ID) Card rack (.)3-3

- Card )( (AST) (.)3-3
()3-3
- Rack (.)3-4
()3-5
()3-4
- LAB ID Bench .Tech
01
Card Barcode Rack .
()3-7
()3-6
(.)3-8
()3-8
00
03
:Phoenix
()3-9
:
.Panel ( )ID .Bacterial identification ( )AST .Antimicrobial susceptibly testing :
-1 ( )ID
.Nephelometer
()3-10
-0 ( )AST indicator 52
( )ID ( .)AST ()3-11
04
()3-11
-3 Panel
, Panel .
()3-13
()3-12
panel
panel .
05
06
):Manual (AST
Swab
Mueller Hinton .
Antibiotic disks
04 37.
()3-14
07
Antibiotics
.
:Gram +ve
Rifampicin (RA),Vancomycin (VA), Gentamicin (GM) : Staph.
Cefoxitin or Oxacillin + Fusidic Acid (FA), Teicoplanin (TEC)
G+ve disk+X2 : Strept
:Gram ve
X1, X2, X3
Urine disk
X3
X2
X1
G+ve Disk
Nalidexic
Colistin
Imipenem
Cephalaxin Pencillin g (PG)
acid (NA)
(CT)
(IMI)
(CF)
Nitrofurantoi Ceftriaxone Ciprofloxaci
Ampicillin Erythromycin
n (NI)
(CRO)
n (CIP)
(AMP)
(E)
Cephalothin Cefotaxime Ceftazidime
Augmentin
Ampicillin
(KF)
(CTX)
(CAZ)
(AUG)
(AMP)
Ampicillin Meropene
Amikacin Cotrimoxazol Cephalothin
(AMP)
m (MEM)
(AK)
e (SXT)
(KF)
(TS)
Tazobacta Azotreonam
Gentamicin
(TS)
Cotrimoxazol
m (TAZ)
(ATM)
(GM) Cotrimoxazol
e
e
Norfloxacin Cefuroxime Piperacillin
Cefoxitin
Augmentin
(NOR)
(CXM)
(PIP)
(FOX)
(AUG)
Vancomycin
(VA)
01
) )(SBI
:
G-ve .G+ve
: : Specimen Slide
-
Crystal violet
.
Iodine .
Alchohol .
.
Safranine .

.
: Blood Agar Macconkey

Mac .
work sheet :
)Heavy growth of (GNB) or (GPC
)Moderate growth of (GNB) or (GPC
No growth
3 Mix growth.
Catheter tip:
)Significant/ NO Sig. growth of (GNB) or (GPC
09
:G +ve
:
Catalase Test -1
Staphylococcus Streptococcus
:
Catalase .
Loop .
:
Staphylococcus
()4-1
)Strept. (-) Staph. (+

Catalase Positive = Staphylococcus :Coagulase test )1
Staph.aureus
:
Staph Latex .
.
32
()4-2
Staph.aureus = (+) Agglutination

)0 Dnase test
Dnase agar 04. - HCL 12.
()4-3
)Staph.aureus = positive (+
)C.N.S = negative (-
Novobiocin sensitivity Disk )3

Staph.epidermidis , Staph.saprophyticus
()4-4
31
Staph.saprophyticus = Resistant
Staph.epidermidis = sensitive
.Phoenix or Vitek (ID), (AST) for Gram positive ) (AST ) )Manual Mueller hinton
.
( )R (.)S
30
Catalase Negative = Streptococcus : B.A :

: -Hemolytic -1
:
Streptococcus pneumonia, Streptococcus viridans
:
)1 (:Taxos p )Optochin Disk Susciptibility

Streptococcus pneumonia ( )4-6
Streptococcus viridans (.)4-5
()4-6
()4-5
)0 Bile Solubility
Streptococcus pneumonia
:
Bile Solubility Streptococcus
( Streptococcus pneumonia = )+ (.)4-7
33
)4-7(
:-Hemolytic -0
.
Group A, Group B, Group C, Group F, Group G :
)4-8(
:
:Taxos a (Bacitracin disk Susciptibility) ) 1
S.pyogenes , S.agalactiae
)4-9(
34
Sensitive = S.pyogenes
Resistant = S.agalactiae
Camp Test )0
Strep.agalactiae Group B
:
-1 Staph.aureus . B.A
-0 Group B
.Staph.aureus
-3 24.
:
Group B
()4-10
)3 Lancefield Grouping Masta strep
()4-11
35
.
Latex card
.
Sticks . Loop
.
Agglutination : . A
()4-12
Gamma-hemolytic -3
Enterococci B.A
()4-13
:
)1 bile esculin, Nacl
36
:
Bile esculine 04 )Positive (+
()4-14
6.5% Nacl 04
.
()4-15
()+
()-
Enterococci
Strep.Group D
grouping
grouping
Bile esculin
+
+
+
37
6.5% Nacl
+
+
Phoenix or Vitek (ID), (AST) for Gram positive ) (AST ) )Manual )X2, GPC disk( B.A
.
()4-16
( )R (.)S
31
G-ve

:
()4-17
= Lactose Fermentation positive MAC

= Lactose Fermentation negative MAC
:
Oxidase Test )1
Oxidase Swab .
()4-18
= )Oxidase positive (+
= )Oxidase negative (-
39
)0 API 20E
Enterococci
G ve bacilli
02 ( )Micro tube
.
5
.
( )3-0

5
.
.
.
:
| |MAN| |CIT| |VP
.
()4-19

ADH, URE
42
()4-20

37 04.

.
()4-21
41
TESTS
SUBSTRATE
REACTION TESTED
- RESULTS
*negative*
+ RESULTS
*positive*
ONPG
ONPG
beta-galactosidase
colorless
Yellow
ADH
arginine
arginine dihydrolase
Yellow
red/orange
LDC
lysine
lysine decarboxylase
Yellow
red/orange
ODC
ornithine
ornithine
decarboxylase
Yellow
red/orange
CIT
citrate
citrate utilization
pale
green/yellow
bluegreen/blue
H2S
Na
thiosulfate
H2S production
colorless/gray
black deposit
URE
urea
urea hydrolysis
Yellow
red/orange
TDA
tryptophan
deaminase
Yellow
brown-red
IND
tryptophan
indole production
Yellow
red (2 min.)
VP
Na pyruvate
acetoin production
colorless
pink/red (10
min.)
GEL
charcoal
gelatin
gelatinase
no diffusion of
black
black diffuse
GLU
glucose
fermentation/oxidation
blue/blue-green
yellow
MAN
mannitol
blue/blue-green
yellow
INO
inositol
blue/blue-green
yellow
SOR
sorbitol
blue/blue-green
yellow
RHA
rhamnose
fermentation/oxidatio
blue/blue-green
yellow
SAC
sucrose
fermentation/oxid
ation
blue/blue-green
yellow
MEL
melibiose
blue/blue-green
yellow
AMY
amygdalin
blue/blue-green
Yellow
ARA
arabinose
blue/blue-green
Yellow
)4-22(
API 20E
Species and subspecies identification of Enterobacteriacae and groups and

species identification of non-fermenting
API 20NE
Identification of gram-negative non-Enterobacteriaceae
API Rapid
20E
Identification of Enterobacteriaceae
API NH
Identification of Branhamella catarrhalis and Neisseria haemophillus
RAPIDEC
Staph
Identification of the commonly occurring staphylococci
API 20 Strep
Identification of streptococci and enterococcs
API Staph
Identification of clinical staphylococci and micrococci
API Coryne
Identification of Corynebacteria and coryne-like organisms
API 20A
Identification of anaerobes
)4-23(
40
Phoenix or Vitek (ID), (AST) for Gram negative ) (AST ) )Manual Mueller Hinton ( )X1, X2, X3
.
()4-24
Purity Plate .CBA - Pseudomonas spp ( )X2, X3 X1
( )R (.)S
43
44

:
Staphylococcus aureus -1
Staphylococcus saprophyticus -0
Enterococcus -3
Clostridium spp. -4
Pseudomonas spp. -5
Proteus spp. -6
Klebsiella -7
E.coli -1
Acinetobacter baumannii -9
Morganella morgannii
-12
Neisseria spp.
-11
Haemophillus spp.
-10
Cnadida spp.
-13
:
B.A ) Cocci(
(Beta-hemolytic) ( Catalase)
)4-25(
:Diagnosis
" " Catalase test ) 1
45
Positive (+) = Staphylococci

" " Coagulase test ) 0
)4-26(
Positive (+) = Staphylococcus aureus
" " Dnase test ) 3
Staph.aureus = positive (+)
Vitek or Phoenix (ID), (AST) for Gram positive )4

. Genus
.MRSA Oxacillin or cefoxictin, methicillin -
Staphylococcus saprophyticus -0
. , B.A ( Cocci)
:Diagnosis
" " Catalase test ) 1
Positive (+)
" " Coagulase test ) 0
Negative (-)
" " Novobiocin disk sensitivity ) 3
Novobiocin disk = Resistant = Staph.saprophyticus
. Genus
46
Enterococcus -3
) (Cocci ) (Catalase
) (Gamma-hemolytic B.A .
:Diagnosis
)1 Bile esculine " 6.5% Nacl "
Bile esculine (+) 6.5% Nacl (+) = Enterococcus
Genus .
Clostridium spp. -4
) (Bacilli ) (Sporulating
) (Motile .Catalase Negative
()4-28
()4-27
:Diagnosis
)1 :
C.tetani
)0 " Catalase test "
Negatve (-) = Clostridium
: Morphology )3
47

" " Camp test )4
Camp test = positive = Clostridium sp.
)4-29(
API 20A )5
. Genus
Pseudomonas spp -5
Beta- Oxidase Positive /Negative
.)) ( P.aeroginosa( hemolytic
)4-30(
:Diagnosis
:Oxidase test ) 1
Pseudomonas = Oxidase Positive
41
:Morphology )0
( ).
API 20NE )3
Vitek or Phoenix (ID), (AST) for Gram Negative )4
Genus .
Proteus spp. -6
( )Bacilli ) (MAC), (B.A
) (Swarming .
()4-31
:Diagnosis
)1 Oxidase test
Negative (-) = Proteus sp.
:Morphology )0
( ) .
)3 Indole test
49
()4-32
)Proteus vulgaris = Positive (+
)Proteus mirabilis = Negative (-
API 20E )4
Genus .
Klebsiella -7
( )Bacilli
) (Mucoid ) (Capsule .
()4-33
:Diagnosis
:Morphology )1
.
Oxidase test )0
52
Negative (-) = Klebsiella

API 20E )3
. Genus
E.coli -1
(B.A), ( Bacilli(
.MAC Flat ( MAC)
)4-34(
:Diagnosis
Indole test )1
)4-35(
Positive (+) = E.coli

Oxidase test ) 0
Negative (-) = E.coli
51
:Morphology )3

( Macconkey) 42
API 20E )4
Genus .
Acinetobacter bumannii -9
Coccobacillus .
()4-36
:Diagnosis
)1
)0 MAC
Indole, Oxidase tests )3

)Negative (-
Catalase test )4
)Positive (+
API 20NE )5
Vitek or Phoenix (ID), (AST) for Gram negative )6
Genus .
50
Morganella morgannii -12

.Swarming
()4-37
:Diagnosis
)1
Oxidase test )0
Negative
Catalase, Indole tests )3
Positive
Genus .
Neisseria spp. -11

Diplococci ,
B.A, CBA, MAC , .
53
)4-38(
:Diagnosis
.) 1
)4-39(
Thayer Martin Agar, New York Agar ) 0

Neisseria spp. Selective
)4-40(
Oxidase Test )3
Positive
Catalase Test )4
Positive
54

Genus .
N.gonorhoeae N.meningitidis Acid production from Glucose, Lactose, Maltose ()
()4-41
Haemophillus spp. -10

Pleomorphic
Coccobacilli ) (X,V factors
, CBA B.A (Staph
) .streak ,
.
()4-42
55
:Diagnosis
) 1
)4-43(
X,V growth factors ) 0

Mueller X, V disks
. 04 , Hinton
)4-44(
Haemophilus species
Growth Around Disk

X
V
XV
+
v/+
+
+
+
+
+
+
+
+
Haemophilus aegyptius
Haemophilus aphrophilus
Haemophilus ducreyi
Haemophilus haemolyticus
Haemophilus influenza
Haemophilus parahaemolyticus
Haemophilus parainfluenzae
)4-45(
56
Oxidase test )3
Positive
Genus .
Candida albicans. -13
()4-46
:Diagnosis
)1 Germ Tube Test
0.5 0-1 Suspension Yeast 3-0
Pasteur pipette Cover slide .
()4-47
Vitek (ID) for Yeast )0
57
) ()Urine Specimen
( )

,

Report
No Growth
No significant Growth
ID (Identification) & sens if
pure
If 2 or more types, mixed
bacterial flora.
Culture is contamination
ID & sens if pure
If 2 or more types, mixed
bacterial flora.
Culture is contamination
-
Colony count
0 cfu
1-10 cfu
11-100 cuf
>100
Catheter Voided .
.Blood Agar, C.L.E.D
4
Work sheet 103 10
.105
Catheter , Significant growth of .. :
No.sig.g .9
Voided : :
.105 GNB
.Pus cells
:
Vitek or Phoenix (ID), (AST) for Gram negative or positive
Genus .
) (AST ) )Manual
X2, ( )GPC( B.A / )U, X2, X3( )GNB( Mueller hinton
)GPC .
51
( )R (.)S
59
E.coli C.L.E.D
()5-1
Vitek or Phoenix (ID), (AST) for Gram negative Genus .
Klebsiella .
()5-2
Vitek or Phoenix (ID), (AST) for Gram negative Genus .
62
Proteus spp. C.L.E.D Swarming
)5-3(
Vitek or Phoenix (ID), (AST) for Gram negative . Genus
Pseudomonas spp. C.L.E.D
)5-4(
Vitek or Phoenix (ID), (AST) for Gram negative . Genus
Staphylococcus saprophyticus (female)

Acinetobacter bumannii
Enterococci
Staphylococcus aureus
61
) ()Stool & Sputum Specimens

Normal Flora
).(Salmonella & Shigella
Stool XLD .Selenite Broth Salmonella Shigella No : Work sheet Salmonella or Shigella isolated Sub culture
.Selenite broth
Salmonella spp. -1

.
()6-1
:Diagnosis
:Morphology )1
XLD .H2S
)0 API 20E
Genus .
60
Shigella spp. -0
.
()6-2
.
:Diagnosis
: Morphology )1
XLD .
API 20E )0
Genus .
63
:Sputum
. Crystal violet Slides
.Epithelial cells Pus cells
.CBA B.A MAC
Heavy,Moderate,Low Growth of Work sheet
.Normal Flora
Neisseria : )6-3( sputum
catarrhalis, Candida albicans,diphtheroids, alphahemolytic streptococci, and some staphylococci
)6-3(
:
Streptococcus pyogenes -1
Beta-hemolytic streptococci -0
Haemophillus influenzae -3
Klebsiella -5
Pseudomonas -6
Candida spp -7
Proteus -1
64
Streptococcus pyogenes Beta-hemolytic Strept
)6-4(
: Diagnosis
Catalase test )1
Negative = no bubbles
:Taxos a (Bacitracin disk Susciptibility) ) 0
""
Sensitive = S.pyogenes
Lancefield grouping ) 3
. Genus
) Manual) ( AST) ) X2, GPC disk( B.A

.
65
( )R (.)S
66
()Blood Culture Specimens

blood culture bottles
,
Bactec alert ( )7-1

.Positive
:
Bactec
()7-2
:
()Patient ID
()Patient name
()Accession
(F11 )Collection Date
( )Bar code .
: F10
()7-2
()7-1

Bar code
( )slot ()7-3
67
( )

( )7-5
( )
,
Bottle .bottle barcode
()7-3
,
.
)Macconkey Agar( , )Chocolate Agar( , )Blood Agar( :
Slide ()G +ve( )G ve
:
-
.
,Streaking
04
slide G ve, G+ve
.
.
) Vitek or Phoenix (ID), (AST Genus
.
61
Manual Gram +ve staph strept :

ve
+ve
+ve
BILE
+or
ve
+ve
NACL
Group latex
A.B.C.D.F.G
Streptococcus
Group D
Enterococcus
sp.p
Organism
Ve
+ve
+ve
DNAS
S or R
FOX
C.N.staph
Normal flora
Staphylococcus
Aureus
MRSA
Organism
( )R (.)S
69
72
Flow charts
)8-1(
)8-2(
71
()8-3
70

,
...

.
73
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2013

(MAC) Macconkey Agar

Sabouraud Dextrose Agar

Selenite Broth Media

)THIO( Thioglycollate broth

Mannitol Salt Agar

Moueller hinton Agar

)TM( Thayer Martin media

: Bile Esculin Agar (BEA)

Todd Hewitt Broth media

( )Pus swab B.A, MAC, CBA

) ()Stool & Sputum Specimens

B.A , CAB , MAC Slide Pus cells,

G ve MAC G +ve / B.A

THIO Sub ( Fluid

rack Vitek (.)3-3 (ID) Card rack (.)3-3

- LAB ID Bench .Tech

Card Barcode Rack .

: Blood Agar Macconkey

)Strept. (-) Staph. (+

Staph.aureus = (+) Agglutination

Novobiocin sensitivity Disk )3

Catalase Negative = Streptococcus : B.A :

= Lactose Fermentation positive MAC

Species and subspecies identification of Enterobacteriacae and groups and

Identification of gram-negative non-Enterobacteriaceae

Identification of Branhamella catarrhalis and Neisseria haemophillus

Identification of the commonly occurring staphylococci

Identification of streptococci and enterococcs

Identification of clinical staphylococci and micrococci

Identification of Corynebacteria and coryne-like organisms

Purity Plate .CBA - Pseudomonas spp ( )X2, X3 X1

Positive (+) = Staphylococci

Vitek or Phoenix (ID), (AST) for Gram positive )4

Negative (-) = Klebsiella

Positive (+) = E.coli

Indole, Oxidase tests )3

Morganella morgannii -12

Neisseria spp. -11

Thayer Martin Agar, New York Agar ) 0

Vitek or Phoenix (ID), (AST) for Gram negative )5

Haemophillus spp. -10

X,V growth factors ) 0

Growth Around Disk

Candida albicans. -13

Vitek (ID) for Yeast )0

Vitek or Phoenix (ID), (AST) for Gram negative Genus .

Vitek or Phoenix (ID), (AST) for Gram negative Genus .

Proteus spp. C.L.E.D Swarming

Vitek or Phoenix (ID), (AST) for Gram negative . Genus

Pseudomonas spp. C.L.E.D

Vitek or Phoenix (ID), (AST) for Gram negative . Genus

Staphylococcus saprophyticus (female)

) ()Stool & Sputum Specimens

Streptococcus pyogenes Beta-hemolytic Strept

) Manual) ( AST) ) X2, GPC disk( B.A

()Blood Culture Specimens

Manual Gram +ve staph strept :

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