B GIO DC V O TO

TRNG I HC NHA TRANG

CNG HO X HI CH NGHA VIT NAM

c lp - T do - Hnh phc

_____________________________________________________

__________________________________________

CNG HC PHN
1.

Thng tin chung

Tn hc phn:

CC PHNG PHP HIN I NG DNG

TRONG PHN TCH THC PHM
Food Modern Analytical Methods

M s:

FS503

Thi lng:

2(1,5-0,5)

Loi:

chuyn ngnh, bt buc

Hc phn tin quyt: khng

2.

Nhm mc tiu:

a1, b1, c1

B mn qun l:

Cng ngh Sau thu hoch

M t

Hc phn cung cp cho hc vin kin thc ct li v cc phng php hin i ng

dng trong phn tch thc phm bao gm: phng php quang ph hp th phn t UV-Vis,
phng php quang ph nguyn t nh quang ph hp th nguyn t AAS, quang ph plasma
ghp cp cm ng (ICP-EAS, ICP-MS), cc phng php sc k hin i (HPLC, GC), cc
phng php phn tch cu trc c bn (ph UV-Vis, ph IR, ph MS, ph NMR, nhiu x tia
X).
3.

Mc tiu
Sau khi kt thc hc phn hc vin c th:
1) Tip cn cc phng php phn tch hin i.
2) ng dng cc k thut hin i trong phn tch nng sn.

4.
TT
1.
1.1.
1.2.
1.3.
1.4.
1.5.

Ni dung
Ch

Phng php quang ph hp th phn t UV-Vis

Ngun gc ph hp th phn t UV-Vis
C s l thuyt ca phng php
S thit b - Cch vn hnh
Cc cch nh lng bng phng php o quang UV-Vis
ng dng phng php o quang UV-Vis trong phn tch
nng sn
2.
Phng php quang ph nguyn t
2.1. Phng php quang ph hp th nguyn t (AAS)
C s l thuyt ca phng php- Nguyn tc nh tnh
v nh lng

Nhm
mc
tiu
1, 2

1, 2

S tit
LT
TH
4

2.2.

3.
3.1.

3.2.

3.3.

4.
4.1.

4.2.

4.3.

4.4.

5.

Gii thiu cc k thut F-AAS, HG-AAS, GF-AAS v

ng dng trong phn tch nng sn
Phng php quang ph pht x nguyn t (EAS)
C s l thuyt ca phng php- Nguyn tc nh tnh
v nh lng
Gii thiu cc thit b F-AES, ICP-AES, ICP-MS
ng dng trong phn tch nng sn
Cc phng php phn tch sc k hin i
i cng v qu trnh tch sc k
Cc khi nim c bn lin quan n qu trnh sc k
Phn loi cc k thut sc k hin i
Cc i lng c bn nh gi hiu qu tch sc k
Phng php HPLC
Nguyn tc
Thit b
ng dng trong phn tch nng sn
Phng php GC
Nguyn tc
Thit b
ng dng trong phn tch thc phm
Phng php phn tch cu trc
Ngun gc ph dao ng IR
Cc tn s hp th IR c trng ca cc nhm chc - Cc
yu t nh hng
ng dng ph IR trong phn tch cu trc phn t
Phng php ph khi lng (MS)
S hnh thnh ph MS Cc k thut ion ha mu v
phn tch ion
C ch phn mnh-Cc yu t nh hng s phn mnh
Thit b
ng dng trong phn tch thc phm
Phng php ph cng hng t ht nhn (NMR)
Ngun gc hnh thnh ph NMR
Khi nim v chuyn dch ha hc Cc yu t nh
hng n chuyn dch ha hc
Cng tin hiu - Tng tc spin-spin- Hng s tch
Thit b - ng dng
Phng php nhiu x tia X
S hnh thnh ph nhiu x tia X
Thit b - Cc k thut o
ng dng ph nhiu x tia X trong phn tch nng sn
(cu trc tinh th)

1, 2

1, 2

Ti liu

1) Nguyn Th Thu Vn (2004), Phn tch nh lng, NXB i hc Quc gia Tp. H
Ch Minh.
2) Phm Lun (2006), Phng php phn tch ph nguyn t, NXB H Quc gia H
Ni.
3) Nguyn nh Triu (2003), Cc phng php vt l ng dng trong ha hc, NXB
i hc Quc gia H Ni.

4) Nguyn nh Triu (2001), Bi tp v thc tp cc phng php ph, NXB i

hc Quc gia H Ni.
5) David Harvey (2000), Modern Analytical Chemistry, Mc Graw-Hill, USA.
6) Jos A. C. Broekaert (2002), Analytical Atomic Spectrometry with Flames and
Plasmas, WILEY-VCH Verlag GmbH, Germany.
7) Michael W. Dong (2006), Modern HPLC for practicing scientists, John Wiley,
USA.
8)Veronika R. Meyer (2000), Practical High-Performance Liquid Chromatography,
3rd-Edition, John Wiley & Sons, G.B.
9) Leo M. L. Nollet (2000), Food Analysis by HPLC, Marcel Dekker Inc., Belgium.
10) Marie-Isabel Aguilar (2004), HPLC of peptides and proteins: methods and
protocols, Humana Press Inc., USA
11) Susan R. Mikkelsen, (2004), Bioanalytical chemistry, John Wiley & Sons, Inc,
USA.
12) Robert L. Grob, Eugene F. Barry (2004), Modern Practice of Gas
Chromatography, John Wiley & Sons, USA.
13) Pradyot Patnaik (2006), Deans Analytical Chemistry Handbook, Mc Graw-Hill,
USA
14) Gnter Gauglitz and Tuan Vo-Dinh (2003), Handbook of Spectroscopy, WILEYVCH Verlag, Germany.
6.

nh gi
6.1.

Thang im nh gi:

1) nh gi kt qu hc tp hc phn ca hc vin (nh gi hc phn) bao gm hai

phn bt buc l nh gi qu trnh v thi kt thc hc phn;
2) im nh gi qu trnh v thi kt thc hc phn theo thang im 10 (t 0 n 10),
cho im chn;
3) im hc phn l tng cc im nh gi qu trnh (c trng s 30%) v im thi
kt thc hc phn (c trng s 70%) nhn vi trng s v c lm trn n phn nguyn.
4) im hc phn t 5 im tr ln l t yu cu.
6.2.
TT

Cc hot ng nh gi:
Hot ng nh gi

Hnh thc nh gi

Trng s

1.

Tiu lun hoc kim tra

chm im

30%

2.

Thi kt thc hc phn

vit hoc vn p

70%
GING VIN BIN SON

TS. V Ngc Bi

TH K HI NG

TS. Mai Th Tuyt Nga

CH TCH HI NG

TS. V Ngc Bi