An ISO 90001 / 14001 / 27001 Certified

DOUBLE BEAM
UV-VIS SPECTROPHOTOMETER
SL 210














Users Manual











ELICO Limited






SAFETY PRECAUTIONS
The following general safety precautions must be observed during all phases of
operation, service and repair of this instrument. Failure to comply with these
precautions or with specific warnings elsewhere in this manual violates safety
standards of design, manufacture and intended use of the instrument. ELICO
assumes no liability for the customers failure to comply with these requirements.

GROUND THE INSTRUMENT
To minimize shock hazard, the instrument chassis and cabinet must be connected
to an electrical ground. The instrument is equipped with a three conductor power
cable. The power cable must either be plugged into an approved three-contact
electrical socket or used with a three-contact to two-contact adapter with the
grounding wire (green) firmly connected to an electrical ground (safety ground) at
the power socket. Mating plug of the power cable should meet relevant
specifications of Bureau of Indian Standards (BIS).

DO NOT OPERATE IN AN EXPLOSIVE ATMOSPHERE
Do not operate the instrument in the presence of inflammable gases or fumes.
Operation of any electrical instrument in such an environment constitutes a definite
safety hazard.

KEEP AWAY FROM LIVE CIRCUITS
Do not install substitute parts or perform any unauthorized modifications to the
instrument. It may be introducing additional hazards. Return the instrument to the
nearest ELICO sales and Support Centre for service and repair to ensure that
safety features are maintained.

FOLLOW THESE SIGNS STRICTLY

NOTE :
NOTE sign denotes important information. It calls attention to
procedure, practice, condition etc., which is essential to highlight.

CAUTION : CAUTION sign denotes a hazard. It calls attention to an operating
procedure, practice, condition etc., which if not correctly performed
or adhered to, could result in damage or destruction of part or all
of the product or an injury to personnel.

WARNING : Sign denotes, Hot surface. It calls attention to practice i.e., not to
be touched. When the flame is ON







USERS MANUAL
232 - UM - 01





DOUBLE BEAM
UV-VIS SPECTROPHOTOMETER

SL 210




APPLICABILITY

This manual applies to instrument
With Serial Number ___________


NOTE

Keep with instrument


WARNING

To reduce risk of electrical fire
or shock hazards, do not expose
this instrument to rain or
excessive moisture.

SL 210 USERS MANUAL


i

INDEX

SECTION CONTENTS PAGE

1 GENERAL INFORMATION 1-1
1.1 INTRODUCTION 1-1
1.2 PRINCIPLE OF OPERATION 1-1
1.3 SPECIFICATIONS 1-1
1.4 INSTRUMENT AND MANUAL IDENTIFICATION 1-2
1.5 RECOMMENDED SPARES 1-2
1.6 SAFETY CONSIDERATIONS 1-2

2 INSTALLATION 2-1
2.1 INTRODUCTION 2-1
2.2 INITIAL INSPECTION 2-1
2.3 INSTALLATION REQUIREMENTS 2-1
2.4 REPACKING FOR SHIPMENT 2-2

3 OPERATION 3-1
3.1 INTRODUCTION 3-1
3.2 DESCRIPTION 3-1
3.3 LAYOUT OF DISPLAY, CONTROLS, READOUTS Etc. ON
PANELS
3-1
3.4 PRE-OPERATING INSTRUCTIONS 3-4

4 OPERATIONAL FLOW 4-1
4.1 INITIALIZATION 4-1
4.2 SPECTRUM 4-2
Spectrum VIEW 4-3
Spectrum PRINT 4-3
- TABLE 4-4
- GRAPH 4-5
ABSORBANCE 4-5
% TRANSMISSION 4-5
4.3 PHOTOMETRIC 4-6
Photometric VIEW 4-7
Photometric PRINT 4-8
4.4 QUANTITATIVE 4-8
STANDARD METHOD 4-9
- SELECTION OF UNITS 4-9
- CALIBRATION 4-10
OPTION OLD/NEW CAL DATA 4-10
ABSORBANCE INPUT
KEYING-IN THE VALUES OF CONCENTRATION OF
STANDARDS IN PPM
4-10
ABSORBANCE INPUT

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INDEX

SECTION CONTENTS PAGE
KEYING-IN THE VALUES OF CONCENTRATION OF
STANDARDS IN MOLES
4-10
MEASURE 4-10
KEY IN 4-11
-FACTOR METHOD 4-12
- MEASUREMENT 4-12
VIEW - DATA OF SAMPLES 4-13
PRINT DATA OF SAMPLES 4-14
AUTOZERO 4-14
FILE 4-14
FILE VIEW 4-15
FILE PRINT 4-15
FILE LOAD 4-15
FILE DELETE 4-16
4.5 SYSTEM MODE 4-16
- LAMPS 4-16
-BASELINE 4-16
-DIAGNOSTICS 4-17

4.6 TIME SCAN 4-17
4.7 STANDARDS PRESENTATION 4-19
VIEW STANDARDS DATA 4-19
SAVE - STANDARDS DATA 4-20
4.8 SAMPLES PRESENTATION 4-21
4.9 MODES 4-21

5. CHECKING THE PERFORMANCE 5-1
5.1 PRE-PERFORMANCE CHECK INSTRUCTIONS 5-1
5.2 SPECTRAL CALIBRATION 5-1
5.3 PHOTOMETRIC PERFORMANCE 5-1
- ABSORBANCE 5-2
- % TRANSMISSION 5-2
- CONCENTRATION 5-2

6. TROUBLE SHOOTING 6-1
ANNEXURES
A THEORY OF COLORIMETRY AND SPECTROSCOPY A-1
B PRINCIPLE OF OPERATION B-1
C SPECIFICATIONS OF SL 210 C-1
D DOUBLE BEAM UV-VIS SPECTROPHOTOMETER SL 210 D-1
E CARE & MAINTENANCE OF CELLS E-1
F ELECTROMAGNETIC SPECTRUM F-1

SL 210 USERS MANUAL


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1. GENERAL INFORMATION

1.1 INTRODUCTION

The information contained in this manual is for installation and operation of
ELICOs MICROPROCESSOR (P) BASED DOUBLE BEAM UV-VIS
SPECTROPHOTOMETER, SL 210. This can also be operated with PC through its
USB Interface with the help of ELICOs Windows based software viz.,
Spectratreats
TM
(optional).

This section of the manual covers the principle of operation, theory of SPECTRO-
PHOTOMETRY, Specifications, Manual identification and other general information
on the instrument.

1.2 PRINCIPLE OF OPERATION

1.2.1 DOUBLE BEAM UV-VIS SPECTROPHOTOMETER, SL 210 works on the
principle of SPECTROPHOTOMETRY based on Beer-Lambert Law.

1.2.2 SPECTROPHOTOMETRY

A brief theory of COLORIMETRY & SPECTROPHOTOMETRY is given at the end
of the manual.

Annexure - A

1.2.3 PRINCIPLE

Monochromatic light passes through media of Reference and standard solutions of
known concentration and the Sample-under-test in identical conditions. The
transmitted light intensities through these media are detected, compared and processed
to present

a. Absorbance and percentage transmission of the sample.
b. Concentration of the sample in comparison to the solution of known
Concentration at the wavelength selected
Annexure - B

1.3 SPECIFICATIONS

Specifications of DOUBLE BEAM UV-VIS SPECTROPHOTOMETER, SL 210 are
given at the end of the manual.

Annexure - C

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1.4 INSTRUMENT AND MANUAL IDENTIFICATION

The instrument is identified by a serial number on the number plate on the rear panel
of the instrument.
The manual applies to the instrument with serial number as indicated on the title page.
1.5 RECOMMENDED SPARES

The following spares are recommended. Available at extra cost:

- 3A 250V cartridge fuse for 90 to 260V AC supply voltage - 2 Nos.
- Deuterium Lamp - 1 No.
- Tungsten Lamp - 1 No.

CAUTION

Do not touch the glass envelopes of the lamps,
particularly quartz window of Deuterium
Lamp.


NOTE

After replacement of Deuterium & Tungsten
Lamps optical alignment may be necessary.
Contact your nearest ELICO Sales & Support
Center.

1.6 SAFETY CONSIDERATIONS

The users manual should be read and safety precautions fully understood before using
the instrument.

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2. INSTALLATION

2.1 INTRODUCTION

This section of the manual contains necessary information and instructions to
install DOUBLE BEAM UV-VIS SPECTROPHOTOMETER, SL 210,
including initial inspection procedures, power & grounding requirements and
instructions to repack the instrument for shipment in the unlikely event of such a
necessity arising.

2.2 INITIAL INSPECTION

This instrument is carefully inspected both mechanically and electrically before
shipment. It should be free of mars, scratches and perfect electrically on receipt.
The instrument should be inspected for any damage that may have occurred
during transit. If the shipping container or packing material is damaged, it should
be kept till the contents of the shipment have been checked as per packing list
and instrument has been mechanically checked. Procedure for checking the
performance of DOUBLE BEAM UV-VIS SPECTROPHOTOMETER, SL 210
is given in section 4. If shipping container is damaged, or contents are
incomplete, or instrument does not function as per performance checks, notify
your nearest ELICO Sales and Support Center. Preserve shipping materials for
inspection.

2.3 INSTALLATION REQUIREMENTS

2.3.1 LOCATION

The location where the DOUBLE BEAM UV-VIS SPECTROPHOTOMETER
SL 210 is proposed to be installed should be

- Free from
Dust
Corrosive gases
Vibration & shocks
- Sheltered from direct sun light
- Away from intense EM & RF Interference
- Well ventilated with ambient temperature between 15C & 35C and
Relative humidity between 45% to 80%.

2.3.2 PLACEMENT OF INSTRUMENT

The instrument should be placed on a sturdy laboratory table with a flat rigid top.
The instrument requires a table space of 600 mm x 500 mm. A clearance of at
least 250 mm is required on the sides and rear side of the instrument for proper
ventilation and operational convenience.

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NOTE
The clearance in the rear and on the fan-side of
instrument is essential to ensure proper
ventilation for the heat generating components
inside.

The instrument is provided with soft, insulating & non-scratching feet and a
three-conductor power cord with a molded 3 pin 5A-which when plugged into an
appropriate power outlet grounds the instrument (panel, cabinet and chassis).

NOTE
Ensure that correct fuse is installed in the fuse
holder on the rear side of the instrument.

2.3.3 POWER REQUIREMENT

DOUBLE BEAM UV-VIS SPECTROPHOTOMETER SL 210 requires power
supply of 90-260V, 50 Hz, single phase in 3 contact 5A AC line-filtered power
outlets conforming to relevant specification of Bureau of Indian Standards (BIS),
preferably with switch, fuse and indicator. Maximum power consumption of the
instrument is 170 VA.

NOTE
If Printer is proposed to be used provision for
the printer and line-filtered power for it are also
to be made by the user.

2.4 REPACKING FOR SHIPMENT

NOTE
If the instrument is to be shipped to ELICO for
service or repair, attach a tag to the instrument
identifying the owner and indicating type
number and serial number of the instrument and
service or repair to be carried out. In case of
repair, a detailed note on the defects observed
must be sent along with the instrument.

Remove the 3 contact-molded plug of the instrument from the power outlet, and
also from the power receptacle of the instrument

Place the instrument wrapped in heavy plastic sheet, in the original container,
with original packing material and secured as it was originally received.

If the original container is not available, wrap the instrument in heavy plastic
sheet and pack in shipping container in a manner similar to that in which it was
received.
Mark the shipping container DELICATE INSTRUMENT, FRAGILE etc.,

SL 210 USERS MANUAL


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3. OPERATION


3.1 INTRODUCTION

This section of manual deals with the information, instructions for operation of
DOUBLE BEAM UV-VIS SPECTROPHOTOMETER SL 210 for measurement
and analysis of Absorbance, Percentage transmission & Concentration of Samples.

3.2 DESCRIPTION

3.2.1 DOUBLE BEAM UV-VIS SPECTROPHOTOMETER, SL 210 is a table top,
ergonomically engineered instrument with its components and modules inside laid
out for easy maintainability and fully automatic operation through the keyboard -
the only controls available on its panels.
Annexure D


A selectable light beam in Ultra-violet or Visible spectrum from the LIGHT
SOURCE module gets focused on to the entrance slit of MONOCHROMATOR
module. The incident beam of light gets tuned to keyboard-settable wavelength by
motorized slewing of the holographic grating in Czerny-Turner mount and reflecting
optics and emerges as a monochromatic light of narrow band width from the exit slit
of the Monochromator. This narrow band light from monochromator is switched
between two paths by a rotating chopper - one for REFERENCE CELL and other
for SAMPLE CELL. The transmitted portion of the light through the media
(BLANK, STANDARD & SAMPLE SOLUTION) in the two paths is combined
onto a single photo detector. The output of DETECTOR-PRE-AMPLIFIER module
is processed in the ELECTRONICS module and the
ABSORBANCE/PERCENTAGE TRANSMISSION/ CONCENTRATION of the
SAMPLE medium is present in Scan, Photometric, and Concentration, Time Scan
modes.
With self-check & self-diagnostic facility on LCD read out for monitoring and on
printer for hard copy.

3.3 LAY OUT OF DISPLAY, CONTROLS, READOUTS Etc. ON PANELS

3.3.1 FRONT PANEL
READOUT (1) READOUT - 20 x 4 DOT MATRIX ALPHA NUMERIC
LCD DISPLAY OF 2.95 x 4.75 SIZE CHARACTERS - To
display prompts - for user friendly interaction, entered data and
values of the measured outputs.

NUMBER
KEYBOARD
(2) KEYBOARD - Input device through which different
operations can be made. The function of each key is given
below:

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ALPHANUMERIC KEYS
0/*() (2.1) 0/*( )

1/STU (2.2) 1/STU

2/VWX (2.3) 2/VWX

3/YZ (2.4) 3/YZ

4/JKL (2.5) 4/JKL

5/MNO (2.6) 5/MNO

6/PQR (2.7) 6/PQR

7/ABC (2.8) 7/ABC

8/DEF (2.9) 8/DEF

9/GHI (2.10) 9/GHI


FUNCTION KEYS
(2.11) DOWN - To decrease the value of parameter set on the
READOUT in specific steps.
In case of character entry moves the cursor backward by one
position.

(2.12) UP To increase the value of parameter set on the READOUT
in specific steps.
In case of character entry moves the cursor forward by one
position.

NEXT/- (2.13) NEXT - To select the next step (Eg. next value of standard and
its concentration etc.) or to enter the decimal point in the
Concentration value.
In case of character entry this key used to change the letter case
and to select the symbols mode.

ESC (2.14) ESCAPE - To quit from the present operational sequence and
go back to previous operation.

NO/- (2.15) NO - To express negative response to the prompts or to enter
the negative sign of the exponent of Concentration value.
In case of character entry deletes single character.

YES/+ (2.16) YES - To express positive response to the prompts or to enter
the positive sign of the exponent of Concentration value.
In case of character entry this key used to enter into symbols
menu.

ENTER (2.17) ENTER - To enable the instrument accept/enter the data
parameter set or to go to the next operation.

MODE (2.18) MODE - To select different modes of operations.

SL 210 USERS MANUAL


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3.3.1.1 PROCEDURE TO ENTER CHARACTERS

1. Pressing a key scrolls characters present on it.
Ex: Repeated pressing key 8 will scroll D, E, and F, 8 on display.
2. If no other key is pressed for a period, then cursor will advance to next position.
i.e character is accepted for that position.
3. When characters required are present on two different keys then they can be pressed
one after other without waiting.
4. Keep pressing on a key for more than a period of time, this will select number present
on that key, and advances to next location.

Symbols Mode
1. Press NXT key till Sym is appeared in display.
2. Press YES key to enter into the symbols menu.
3. Press appropriate number to select the corresponding symbol.

3.3.2 TOP PANEL
SAMPLE
COMPARTMENT
LID
(3) LID - To be lifted to get access to cuvettes in sample
compartment. A shutter automatically protects the detector
from extraneous light whenever the LID is opened.

3.3.3 REAR PANEL
90-260V AC (4) LINE FILTERED POWER RECEPTACLE - To connect
the socket end of the three-conductor power cord of the
instrument with full cartridge.

ON (5) POWER OFF/ON SWITCH - to switch the instrument ON
or OFF.

(6) NUMBER PLATE - To indicate the serial number of the
instrument.


3.3.4 VENTILLATION FAN-RIGHTSIDE PANEL

PRINTER (7) PRINTER PORT - To connect the printer.

USB (8) PC CONTROL - To connect PC to Instrument

(9) FUSE RATING STICKER - To indicates the voltage and
power rating.

(Numbers in brackets of controls and readouts etc., on front and rear panel of the instrument
are referred in Annexure - D)

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3 4

3.4 PRE-OPERATING INSTRUCTIONS

3.4.1 Ensure

90-260V, 50 Hz, single-phase power supply is available in the 3 contact 5A power
outlet.

Cartridge fuses of 3A, 250V AC is in its place in the instrument.

The socket of the power cord of the instrument is inserted properly into the power
receptacle at the rear side of the instrument-end and its 3 contact plug into line-
filtered 3 contact 5A power outlet to power it.

NOTE

In case printer is proposed to be used, ensure the
socket of its power cord is properly inserted in
the power receptacle of the printer and the 3
contact plugs into line-filtered 3 contact 5A
power outlets.

The floor of the Sample compartment is clean
and devoid of any split liquid to eliminate the
possibility of the vapors of split liquids vitiating
the results.


3.4.2 Keep the REFERENCE SOLUTION & SAMPLE-UNDER-TEST handy.

NOTE

One REFERENCE-filled and One SAMPLE-
filled cuvettes as default in the instrument and
*One REFERENCE-filled and up to 6 SAMPLE
filled cuvettes in photometric, concentration mode
and 4 SAMPLE filled cuvettes in spectrum mode
can be used in the first instance. (*Optional)

The cuvettes should be thoroughly clean and
devoid of fingerprints.

Annexure - E

SL 210 USERS MANUAL


4 1


4.0 OPERATION FLOW

4.1 INITIALIZATION:
Switch ON the Instrument
4.1 (Screen) You find the Message on LCD as
Proceed to 4.1.1
ELICO * SL 210*
DOUBLE BEAM UV-VIS
SPECTROPHOTOMETER
Release Vx. xxRxx PC

Followed by

4.1.1 (Screen) You find the Message on LCD as ELICO * SL 210*
DOUBLE BEAM UV-VIS
System is starting
Please wait
Followed by
Lamp Test:
4.1.2
(Screen)
< ENT > Accept and Proceed. ELICO * SL 210*
DOUBLE BEAM UV-VIS
Remove Cuvttes in
REF, SMP Holders <ENT>

4.1.3
(Screen)
You find the Message on LCD as ELICO * SL 210*
DOUBLE BEAM UV-VIS
D2 Lamp testing
Alignment in process
Followed by
4.1.4
(Screen)
Test the D2 Lamp and display
the Message on LCD as
ELICO * SL 210*
DOUBLE BEAM UV-VIS
D2 Alignment OK
Auto cal. In progress
Followed by
4.1.5
(Screen)
Display the Message after
completion
of the Lamps Test.
ELICO * SL 210*
DOUBLE BEAM UV-VIS
W Lamp Alignment OK
* Lamps Test Over *
Baseline Scan:

4.1.6
(Screen)
< YES > Go to 4.1.7
< NO > Go to 4.1.9
ELICO * SL 210*
DOUBLE BEAM UV-VIS
Start Base line Scan?
<YES/NO>

4.1.7
(Screen)
Display the Message till an instrument
generates its baseline data.
ELICO * SL 210*
DOUBLE BEAM UV-VIS
BASE LINE SCAN Under
Progress
Followed by
4.1.8
(Screen)
You find the Message on LCD as
Press Any Key to Proceed.
ELICO * SL 210*
DOUBLE BEAM UV-VIS
Base Line Scan Over
Press any key

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Main Menu:
4.1.9
(Menu)





You find the Message on LCD as.

-------------* MENU *--------------
1. SPECTRUM 2. MULTI
3.TIMESCAN 4. SYSTEM
5. QUANTITATIVE 6. PC
Note: To use any of modes available you need
to perform FULL RANGE baseline scan at
least once.

4.2 SPECTRUM:


Press <1> key, if scanning is desired in
Modes Menu

4.2.1
(Screen)
Default SWL = 190.0 nm
< # > Wavelength Entry
< NO > Clear the field entry pointed
< ESC > Go to Main Menu.
< ENT > Accept the Values and
Proceed.
--------------* Spectrum *----------
Enter scan limits
SWL: 190.0 nm
EWL: nm <#,ENT>

4.2.2
(Screen)
<YES> AZ Select and proceed to 4.2. 3
<NO>AZ Not Select and proceed to 4.2.7
-------------* Spectrum *----------
You Want Auto Zero
Option?
<YES/NO>

4.2.3
(Screen)
< ENT > Accepts the Reference and
Proceed.
< ESC > Go to 4.2.2
--------------* Spectrum *----------
Place REF & SMP
Cuvettes with BLANK
<ENT/ESC>
Followed by
4.2.4
(Screen)
Display the message
For a while
--------------* Spectrum *----------

Preparing to read
Please wait
Followed by
4.2.5
(Screen)


Display the Message While
Scanning REF CUVETTES
Percentage progress also displayed.
--------------* Spectrum *----------
RANGE: (XXX.X - XXX.X)
Scanning REF
Progress xx %
Followed by
4.2.6
(Screen)
< ENT > Accepts the SAMPLE and
proceed to 4.2.8

--------------* Spectrum *----------
Place the Sample


<ENT>


4.2.7
<ENT> Accepts the REF & SAMPLE and
proceed
--------------* Spectrum *----------
Place REF & SMP

<ENT>

SL 210 USERS MANUAL


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4.2.8
(Screen)
Display the message For a while

--------------* Spectrum *----------

Preparing to read
Please wait
Followed by
4.2.8.1
(Screen)
Display the Message While
Scanning SAMPLE CUVETTES.
Percentage progress also displayed.
--------------* Spectrum *----------
RANGE: XXX.X - XXX.X
Scanning SMP
Progress xx %
Wait till you find the following on LCD
display

4.2.9
(Menu)
< # > Take Choice. --------------* Spectrum *----------
1.VIEW 4. MENU
2.PRINT 5. AUTOZERO
3. REPEAT <#>
4.2.9.1
Spectrum VIEW:

Press <1> key, if it is desired to view the
Scan data, If AZ not Selected you finds the
message on LCD as.

4.2.9.1.1
(Screen)
<?, ?> ? increment, decrement
< # > ? Entry
< ESC > Go to 4.2.9
--------------* Spectrum *----------
: XXX.X nm AZ <OFF>
Abs = Y.YYYY
%T = YY.Y < #, ? , ? >
If AZ Selected you find the message on
LCD as.

4.2.9.1.2
(Screen)
<?, ?> ? increment, decrement
< # > ? Entry
< ESC > Go to 4.2.9
<NXT> Display AZ status ON and
OFF Results.
--------------* Spectrum *----------
: XXX.X nm AZ <ON >
Abs = Y.YYYY
%T = YY.Y < #, ? , ? , NXT >
4.2.9.2 Press <4> key, if it is desired to quit

4.2.9.2.1
(Screen)
< YES > Go to Main Menu.
< NO > Go to 4.2.9
--------------* Spectrum *----------
DATA will be LOST! OK

<YES/NO>

4.2.9.3
Spectrum PRINT:

4.2.9.3.1
(Screen)
Press <2> key, if it is desired to print data --------------* Spectrum *----------
1.VIEW 4. MENU
2.PRINT 5. AUTOZERO
3.REPEAT
Followed by

If AZ Selected Proceed to 4.2.9.3.2
If AZ Not Selected Proceed to
4.2.9.3.3

4.2.9.3.2
(Screen)


<NXT> Change AZ Status ON and
OFF.
<ENT> Accept AZ Status and
Proceed
------------* Spectrum *----------
Select AZ Status
To Process DATA
AZ < ON > < NXT, ENT >

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4.2.9.3.3
(Screen)
Press <1> to print with HP printer
Press <2> to print with other than HP.
------------* Spectrum *----------
Select Printer
1. HP 2. Others
Select option <#>

If Printer is Ready Proceed to 4.2.9.3.5
If Printer is Not Ready Proceed to
4.2.9.3.4

4.2.9.3.4
(Screen)

Press <1> to check printer again
Press <2> to back to 4.2.9
------------* Spectrum *----------
PRINTER NOT READY
1. Retry
2. Cancel < # >


4.2.9.3.5
(Screen)

< YES > Go to 4.2.9.3.6
< NO > Quit and Proceed.
------------* Spectrum *----------

WISH TO CHANGE NAMES
<YES/NO>

4.2.9.3.6
(Screen)

< ENT > Proceed to Enter the Analyst
Name.
------------* Spectrum *----------
ENTER ANALYST NAME
---
<ENT/ESC/#>

4.2.9.3.7
(Screen)

< ENT > Enter Organization name and
Proceed.
------------* Spectrum *----------
ENTER ORGANISATION:
---
<ENT/ESC/#>

4.2.9.3.8
(Screen)

< ENT > Enter Reference name
and Proceed.

------------* Spectrum *----------
ENTER REFERENCE:
---
<ENT/ESC/#>

4.2.9.3.9
(Screen)

< ENT > Enter Sample name and
Proceed.
------------* Spectrum *----------
ENTER SAMPLE:
--
<ENT/ESC/#>

4.2.9.3.10
(Screen)
Displays the Date ,Time & corresponding
day and goes to 4.2.9.3.11
------------* Spectrum *----------
Xx-xx-xxxx
DAY
Y-YY-YY

4.2.9.3.11
(Screen)

Enter Wavelength to Print and Press
<ENT>
To proceed.

------------* Spectrum *----------
PRINT RANGE
SWL=XXX.Xnm
EWL=XXX.Xnm
<#, ENT>

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4.2.9.3.12
(Screen)

Press <1> to select Abs and go to
4.2.9.3.13
Press <2> to select %T and go to
4.2.9.3.19

------------* Spectrum *---------
-
Select Data To Print
1.Abs 2. %T
Select Option?
<#>
ABSORBANCE:
4.2.9.3.13
(Screen)

Press <1> Key, if DATA is to be printed
and go to 4.2.9.3.14
Press <2> Key, if GRAPH is to be printed
and go to 4.2.9.3.16
------------* Spectrum *----------
Select Print Option
1.TABLE
2.GRAPH < # >

4.2.9.3.14
(Screen)

Enter Print Interval ------------* Spectrum *----------
Enter Print Interval
Range (0.1-1.0) nm :
< #, ENT >
Followed by
4.2.9.3.15
(Screen)

Display the message as ------------* Spectrum *----------
Printing
Under Progress
Please wait
Followed by With * blinking.
4.2.9.3.16
(Screen)

Enter Lower Limit and Higher Limit &
press <ENT>
------------* Spectrum *----------
Enter Abs Axis Limits
LO: HI:
<#,ENT>
Followed by
4.2.9.3.17
(Screen)

Displays the message for a while ------------* Spectrum *----------
Calculation
Under Progress.
Please wait

4.2.9.3.18
(Screen)
After completing calculation it prints the
Graph and goes to 4.2.9
--------------* Spectrum *----------
Printing
Under Progress
Please wait
% Transmission:
Press < 2 > key, to select %T in 4.2.9.3.12
4.2.9.3.19
(Screen)
Press <1> if data is to be printed & go to
4.2.9.3.14
Press <2> if data is to be printed & go to
4.2.9.3.20
------------* Spectrum *----------
Select print option
1.Table
2.Graph
< # >

4.2.9.3.20
(Screen)
Enter Lower Limit and Higher Limit & press
<ENT>
--------------* Spectrum *----------
Enter %T Axis Limits
LO: HI:
<#,ENT>

SL 210 USERS MANUAL


4 6


4.2.9.3.21
(Screen)
Displays the message for while --------------* Spectrum *----------
Calculation
Under Progress.
PLEASE WAIT

With * blinking.
4.2.9.3.22
(Screen)
After completing calculation it prints the
Graph
--------------* Spectrum *----------

PRINTING under
Progress. PLEASE AIT
Followed after printing and goes to 4.2.9
4.2.9.3.23
(Screen)
Press <4> to Quit from Spectrum mode
Press<YES> to Quit
Press<NO>to remain in the same mode
--------------* Spectrum *
Data will be Lost ! OK
<YES/NO>

4.3
PHOTOMETRIC:

4.3.1
(Menu)
Press <2> key, if the photometric mode is
desired
-------------* MENU *--------------
1. SPECTRUM 2. MULTI
3.TIMESCAN 4. SYSTEM
5. QUANTITATIVE 6. PC

4.3.2
(Screen)
< # > Wavelength Entry
< ENT > Accept the Values and
Proceed
<NO > Stops Entry and Proceed.
< ESC > Go to Main Modes.
------------*Multi *-------------
Enter Wavelengths.
01: xxx nm
(MAX-16) < #, ENT, NO >


With * blinking.

4.3.3
(Screen)
< YES > Go to 4.3.2
< NO > Proceed to 4.3.4.
------------*Multi *-------------
XX * s entered. Wish
to change/check ?
<YES/NO>
With * blinking.
4.3.4
(Screen)
< # > Entry of Samples number
< ENT > Accept and Proceed.
------------*Multi *-------------
Enter No. of Samples
to be scanned: X
(MAX 6) < #, ENT >

4.3.5
(Screen)
<YES> AZ Selected and Proceed to 4.3.6
<NO> AZ Not Selected and Proceed to
4.3.11
------------*Multi *-------------
You Want Auto Zero
Option? <YES/NO>

4.3.6
(Screen)

<ENT> Accept REF in Both Cuvettes
and Proceed.
< ESC > Go to 4.3.5

------------*Multi *-------------
Place REF & SMP
Cuvettes with BLANK
<ENT/ESC>

SL 210 USERS MANUAL


4 7


4.3.7
(Screen)

Display Message on LCD for a While. ------------*Multi *-------------
Preparing to read
Please wait..
Followed by
4.3.8
(Screen)
Display Message on LCD
While Reading REF Cuvettes.
------------*Multi *-------------
Reading REF XX of XX
At XXX.X nm
Please Wait . . .

4.3.9
(Screen)

If Number of References more than one you
find the Message on LCD. Or Else Proceed to
4.3.10
< ENT > Go to 4.3.7.and return to 4.3.10
------------*Multi *-------------

Place next BLANK
< ENT >

4.3.10
(Screen)

< ENT > Accept the Samples and
Proceed to 4.3.12
------------*Multi *-------------
Place the Samples

<ENT>

4.3.11 <ENT> Accept the Samples and proceed.
<ESC> Go to 4.3.5
------------*Multi *-------------
Place Ref & Smp

<ENT/ESC>

4.3.12
(Screen)
Display Message on LCD
for a While.
------------*Multi *-------------
Preparing to read
Please wait.

Followed by
4.3.13
(Screen)
Display Message on LCD
While Reading SAMPLE.
------------*Multi *-------------
Reading SAMPLE XX of XX
At XXX.X nm
Please Wait . . .

4.3.14
(Screen)


If No of Samples more than one you find the
Message on LCD. Or Else Proceed to 4.3.15.
< ENT > Go to 4.3.12
------------*Multi *-------------

Place next Sample
< ENT >
Followed by
4.3.15
(Screen)
< # > Take choice. ------------*Multi *-------------
1. VIEW 4. Menu
2. PRINT
3. Auto Zero < # >
4.3.15.1 Photometric VIEW:


4.3.15.1.1
(Screen)
Press <1> key, if it is desired to view the
data
------------*Multi *-------------
1.VIEW 4. Menu
2. PRINT
3. Auto zero <#>

SL 210 USERS MANUAL


4 8


Followed by

If AZ Selected proceed to 4.3.15.1.2
If AZ Not Selected Proceed to 4.3.15.1.3


4.3.15.1.2
(Screen)
< NXT > AZ Status on or off
< ENT > Accept and Proceed to
4.3.15.1.3
< ESC > Go to 4.3.15
------------*Multi *-------------
Select AZ Status
To Process DATA
AZ < ON > < NXT, ENT >
Followed by
4.3.15.1.3
(Screen)
<? , ? >To change Wave Length number
< # > Enter Sample number Directly
< ESC > Go to 4.3.15
<NXT> To view next sample data if any
------------*Multi *-------------
Smp 01 1: XXX.X nm
Abs = Y.YYYY
%T = YY.Y Y < #, ?, ? >

4.3.15.2 Press <4> key, if it is desired to quit from
viewing mode of data presentation

4.3.15.2.1
(Screen)
You find the Message on LCD.
< YES > Go to Main Menu.
< NO > Go to 4.3.15
------------*Multi *-------------
DATA will be LOST ! OK

<YES/NO>

4.3.15.3 Photometric PRINT:

Press <2> key, if it is desired to print data in
4.3.15

4.3.15.3.1
(Screen)
You find the Message on LCD as.
Press <NXT> to select Auto zero
Press <ENT> to accept and proceed.
------------*Multi *-------------
Select AZ status
To process DATA
AZ<ON> <NXT,ENT>
Followed by
4.3.15.3.2
(Screen)
Select 1.HP or 2.Others in select printer
mode and displays the message after showing
screen from 4.2.9.3.4 to 4.2.9.3.10 with multi
mode
------------*Multi *-------------

PRINTING under progress
PLEASE WAIT

After printing Over it goes to 4.3.15
4.3.15.4 Press <3> key, if You want Auto Zero Again.
Proceed to 4.3.15.


4.4
QUANTITATIVE:
Press <5> key, if desired Mode is
Concentration.
-------------* MENU *--------------
1. SPECTRUM 2. MULTI
3.TIMESCAN 4. SYSTEM
5. QUANTITATIVE 6. PC

4.4.1
(Menu)
< # > Enter Choice.
< ESC > Go to Main Menu.

--------*Quantitative*---------
1. Standards
2. -Factor
3. Samples 4. File< # >

SL 210 USERS MANUAL


4 9



STANDARD METHOD:


Press <1> key, if the concentration is desired
to be measured by STANDARD METHOD.




4.4.1.1
(Screen)
< # > Wavelength Entry.
< ENT > Accept and Proceed.
< ESC > Go to 4.4.0
--------*Quantitative*---------

Select : XXX.X nm

< #, ENT >
With * blinking.
4.4.1.2
(Screen)
< # > Enter Standard number.
< ENT > accept and Proceed
< ESC > Go to 4.4.0
--------*Quantitative*---------
Enter Number of
Standards: *
(Max -- 10) < #/ENT >
With * blinking.
NOTE: Minimums of 2 standards are required for the test.
SELECTION OF UNITS:
4.4.1.3
(Screen)
< # > Select units --------*Quantitative*---------
Select Conc. Units
1) Moles
2) ppm
< # >
Moles UNIT SELECTED
Press <1> key, if the units of Concentration
desired M (Moles/litre).

4.4.1.4
(Screen)

< # > Enter Concentration
< ENT > Accept and Proceed
< ESC > Go to 4.4.1.3
--------*Quantitative*---------
ENTER STD 1 CONC.
*E-..M

< #, ENT >
Proceed to step 4.4.1.7 With * blinking.
Press <2> key, if the units of concentration
desired are ppm.

4.4.1.5
(Screen)
< # > Enter Concentration
< ENT > Accept and Proceed
< ESC > Go to 4.4.1.3
--------*Quantitative*---------
ENTER STD 1 CONC.
-------ppm
< #, ENT>


If the calibration data of the standards and the
units of their Concentration are already entered,
this message will displayed

4.4.1.6
(Screen)
< YES > Proceed.
< NO > Go to 4.4.1.5
--------*Quantitative*---------
DO YOU WANT TO USE OLD
CAL DATA!
<YES/NO>

SL 210 USERS MANUAL


4 10


4.4.1.7
(Screen)
< # > Take Choice.
< ENT > Accept and Proceed
--------*Quantitative*---------
Select mode of Abs Input.
1. MEASURE
2. KEY-IN <#>

CALIBRATION:

KEYING-IN THE VALUES OF CONCENTRATION OF STANDARDS IN M.
4.4.1.7.1
(Screen)
< # > Enter Concentration in MOLE.
< +/- > Enter Sign.
< ENT > Accept and Proceed.

--------*Quantitative*---------
ENTER STD 1 CONC.
*E-.M
< #, ENT >
With * blinking.



KEYING-IN THE VALUES OF CONCENTRATION OF STANDARDS IN
PPM
4.4.1.7.2
(Screen)
< # > Enter Concentration. In ppm
< ENT > Accept and Proceed.
--------*Quantitative*---------
ENTER STD 1 CONC.
-*------ppm
< #, ENT>
With * blinking.
4.4.1.8 MEASURE:

Press <1> key in 4.4.1.7 if the Absorbance
input is to be measured from the standard
solution.

4.4.1.8.1
(Screen)
Enter the Number of Samples Analyzed.
< ENT > Accept and Proceed.
--------*Quantitative*---------
Enter no. Of samples
Max- 16 *
< #, ENT >


4.4.1.8.2
(Screen)
< YES > AZ Selected and Proceed to 4.4.1.8.3
< NO > AZ Not Selected and Proceed to
4.4.1.8.8
--------*Quantitative*---------
You Want Auto Zero
Option?
<YES/NO>



4.4.1.8.3
(Screen)
< ENT > Accepts the Reference and
Proceed.
< ESC > Go to 4.4.1.8.2
--------*Quantitative*---------
Place REF & SMP
Curettes with BLANK
<ENT/ESC>


4.4.1.8.4
(Screen)
Display the Message for
a while
--------*Quantitative*---------
Preparing to read
Please wait . . .
Followed by
4.4.1.8.5
(Screen)
Display the Message While
Reading REF CUVETTES.
--------*Quantitative*---------
Reading BLANK X
At XXX.X nm
PLEASE WAIT . . .

BLANK no. and its WL is displayed.
4.4.1.8.6
(Screen)
If Reference More than one, You find
the Message on LCD and Go to 4.4.1.8.5.
If Reading References Over Go to 4.4.1.8.7
--------*Quantitative*---------
Place next BLANK
<ENT>

SL 210 USERS MANUAL


4 11


4.4.1.8.7
(Screen)
< ENT > Accept the standards and Proceed. --------*Quantitative*---------
Place the Standards
< ENT >

4.4.1.8.8
(Screen)
Display the Message for
a while
--------*Quantitative*---------
Preparing to read
Please Wait.
Followed by
4.4.1.8.9
(Screen)
Display the Message
While Reading.
--------*Quantitative*---------
Reading Standard X
At XXX.X nm
PLEASE WAIT . . .


Standards no. and its WL is displayed.


4.4.1.8.10
(Screen)
If Standards More than one, You find the
Message on LCD. And Go to 4.4.1.8.9 after
Press <ENTER> key .
If reading Standards Over Go to 4.4.1.8.11
--------*Quantitative*---------
Place next standard

< ENT >

4.4.1.8.11
(Menu)
Press <1> key, if it is desired to view the
data..
--------*Quantitative*---------
1.VIEW 4. Save
2. Samples 5. Menu
3. Auto Zero < # >
Proceed to step 4.7
4.4.1.8.12
KEY-IN

Press <2> key in 4.4.1.7 to enter into key in
mode.

4.4.1.8.13
(Screen)
Enter Abs values for Standard 1and Press
ENT key.
--------*Quantitative*---------
Enter Abs of STD 01
X.XXX
(-3 to +3)
< #, ENT >



4.4.1.8.14
(Screen)
If more than one standard entered it again
asks to enter Abs value for second
standard and press ENT key.
--------*Quantitative*---------
Enter Abs of STD 02
X.XXX
(-3 to +3) < #, ENT >



4.4.1.8.15
(Screen)
Enter number of samples and press ENT --------*Quantitative*---------
Enter no. of samples
(Max-16):
< #, ENT>



4.4.1.8.16
(Screen)
Display shows the message as --------*Quantitative*---------
Preparing to read
Please wait

SL 210 USERS MANUAL


4 12


4.4.1.8.17
(Screen)
Display shows the message as


--------*Quantitative*---------
You want Auto zero
Option for samples?
<YES/NO>




Proceed to 4.4.1.8.2 to 4.4.1.8.10 (Screen),
IN place of standards it displays samples.

4.4.1.8.18
(Screen)
After completing 4.4.1.8.10 (Screen) it
displays the message as

--------*Quantitative*---------
1.View 4.Menu
2.Print 5.Save
3.Autozero
<# >


Proceed to 4.7
4.4.2.
-FACTOR METHOD:

4.4.2.1 Press <2> key, if concentration is desired to
be measured in -factor method.


With * blinking.
4.4.2.2
(Screen)
< # > Wavelength Entry
< ENT > Accept and Proceed.
< ESC > Go to 4.4.1
--------*Quantitative*---------
SELECT * nm

< # , ENT >


4.4.2.3
(Screen)
< # > Enter Concentration.
< +/- > Enter Sign.
< ENT > Accept and Proceed
--------*Quantitative*---------
ENTER -FACTOR
E - M
< # , ENT >

4.4.2.4
(Screen)
< # > Enter the No of Samples.
< ENT > Accept and Proceed.
< ESC > Go to 4.4.2.2
--------*Quantitative*---------
ENTER NO. OF SAMPLES
Max. -16 *
< # , ENT >
With * blinking.
4.4.2.5
(Screen)


< YES > AZ selected and Go to
4.4.2.6
< NO > AZ Not Selected and Go to
4.4.2.11
--------*Quantitative*---------
You Want Auto Zero Option?

<YES/NO>

4.4.2.6
(Screen)

< ENT > Accept References and
proceed.
< ESC > Go to 4.4.2.5
--------*Quantitative*---------
Place REF & SMP
Cuvettes with BLANK
<ENT/ESC>

4.4.2.7
(Screen)
Display the Message for
a while
--------*Quantitative*---------
Preparing to read
Please wait

SL 210 USERS MANUAL


4 13


4.4.2.8
(Screen)
Display the Message
While Reading
BLANK no. and its WL is displayed.
--------*Quantitative*---------
READING BLANK -x
at xxx.x nm
Please wait . . .

Followed by
4.4.2.9
(Screen)


If Reference More than one, You find the
Message on LCD, and Proceed to 4.4.2.8
If Reading References Over Go to 4.4.2.10
For AZ YES Display the message.
--------*Quantitative*---------
Place next BLANK
<ENT/ESC>

4.4.2.10
(Screen)

For AZ NO Display the message
< ENT > Accept Samples and proceed

--------*Quantitative*---------
Place the Sample
<ENT>

4.4.2.11
(Screen)

Display the Message for
a while

--------*Quantitative*---------
Preparing to read
Please wait

4.4.2.12
(Screen)

Display the Message
While Reading
--------*Quantitative*---------
READING Sample -x
at xxx.x nm
Please wait . . .

Sample no. and its WL is displayed.
4.4.2.13
(Screen)
If Sample More than one, You find the
Message on LCD., and Proceed to 4.4.2.12
If Reading Samples Over Go to 4.4.2.14
--------*Quantitative*---------

PLACE NEXT SAMPLES
< ENT >
Followed by
4.4.2.14
(Menu)
--------*Quantitative*---------
1. VIEW 4. Menu.
2. PRINT
3. Auto Ze ro < # >
4.4.2.15 VIEW - DATA OF SAMPLES:



Press <1> key, if it is desired to view the
samples data. If AZ Selected You find the
Message on LCD.
If AZ Not Selected Go to 4.4.2.17

4.4.2.16
(Screen)
<NXT > AZ Status on or off
< ENT > Accept and Proceed.
< ESC > Go to 4.4.2.14
--------*Quantitative*---------
Select AZ Status
To Process DATA
AZ < ON > < NXT, ENT >

4.4.2.17
(Screen)
<? , ? > To change Sample number.
<#> Enter Sample number
Directly
< ENT > Accept and Proceed.
< ESC > Go to 4.4.2.14
--------*Quantitative*---------
SMP 1 at ?: X.XXX nm
CONC. XXXXXE M
< ? , ? ,# , ENT >

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4 14


4.4.2.18
PRINT - DATA OF SAMPLES:

Press <2> key, if it is desired to print the
data of Sample in 4.4.2.14

4.4.2.19
(Screen)
You Find the Message
on LCD as



--------*Quantitative*---------
1. VIEW 4. Menu.
2. PRINT
3. Auto zero
Results on Printer
If AZ Selected Go to 4.4.2.20
If AZ Not Selected Go to 4.4.2.21

4.4.2.20
(Screen)


< NXT > AZ Status on or off
< ENT > Accept and Proceed.
< ESC > Go to 4.4.2.14
Followed by 4.3.15.3.2
--------*Quantitative*---------
Select AZ Status
To Process DATA
AZ < ON > < NXT, ENT >


4.4.2.21
(Screen)
Display Message while Printing. --------*Quantitative*---------

PRINTINGUNDERPROGRES
PLEASE WAIT
After printing Over Flow goes to
4.4.2.14
NOTE
Ensure Printer is connected, powered and is in
ON-LINE mode. Data is presented in tabular
form only.

4.4.3 AUTOZERO:

Press <3> key in 4.4.2.14, to Enter into Auto
Zero mode
And goes to 4.4.2.6

4.4.4 MODES:

Press <4> key, to quit from the Data
presentation mode.


4.4.4.1
(Screen)
You Find the Message on LCD
< YES > if it is desired to quit and goes
to Main Menu.
< NO > Go to 4.4.2.14
--------*Quantitative*---------
DATA Will be LOST! OK

<YES/NO>


4.4.5 SAMPLES:

Press <3> key, if it is desired to measure the
samples.

4.4.5.1
(Screen)
You Find the Message on LCD.
< ESC > Go to 4.4.1
--------*Quantitative*---------
Load STD File for
Sample measurement
<ESC>
Proceed to 4.4.1.8.15

SL 210 USERS MANUAL


4 15


4.4.6 FILE:

Press <4> key, if it is desired select the file
options in 4.4.1

4.4.6.1
(Screen)
You Find the Message on LCD.
< ESC > Go to 4.4.1
---------- *FILES*----------
1. View
2. Load
3. Print. 4. Delete <#>
4.4.7 FILE VIEW:



Press <1> key, if it is desired to view the data of
the saved files.


4.4.7.1
(Screen)
<? , ? > Select the Desired File
< # > Enter the number of the Desired
File. < ENT > Accept and Proceed.
< ESC > Go to 4.4.6.1
---------- *FILES*----------
File : nnnnn Type :STD/smp
Time : XX-XX Pm
Date :XX.XX.XXXX
4.4.8 FILE PRINT:

Press <3> key, if it is desired to print the data of
the files.

4.4.8.1
(Screen)
<? , ? > Select the Desired File
< # > Enter the number of the Desired
File. < ENT > Accept and Proceed.
< ESC > Go to 4.4.6.1
---------- *FILES*----------
File : nnnnn Type :STD/smp
Time : XX-XX Pm
Date :XX.XX.XXXX
Followed by

4.4.8.2
(Screen)
Display the Message while Printing --------*Quantitative*---------

PRINTINGUNDERPROGRES
PLEASE WAIT

4.4.9 FILE LOAD:

Press <2> key, if it is desired to load the data of
the files.


4.4.9.1
(Screen)
<? , ? > Select the Desired File
< # > Enter the number of the Desired
File. < ENT > Accept and Proceed.
< ESC > Go to 4.4.1
---------- *FILES*----------
File : nnnnn Type :STD/smp
Time : XX-XX Pm
Date :XX.XX.XXXX
4.4.9.2 Press <ENT> key to load the data of the file.
If selected file is sample type go to
4.4.9.3.


4.4.9.3
(Screen)
You Find the Message on Display.
<ESC> Go to 4.4.9.1
---------- *FILES*----------
CANNOT LOAD SAMPLE
FILE FOR CALIBRATION

<ESC>
If selected file is standard type

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4 16


4.4.9.4
(Screen)
Display the Message
While Loading.
---------- *FILES*----------

FILE LOADING
WAIT A MOMENT
Proceed to 4.4.5
4.4.10
FILE DELETE:

Press <4> key, if it is desired to delete the files.
4.4.10.1
(Screen)
<? , ? > Select the Desired File
< # > Enter the number of the Desired
File. < ENT > Accept and Proceed.
< ESC > Go to 4.4.6.1
---------- *FILES*----------
File : nnnnn Type :STD/smp
Time : XX-XX Pm
Date :XX.XX.XXXX

4.4.10.2
(Screen)
< YES > Proceed.
< NO > Go to 4.4.6.1
---------- *FILES*----------
DO YOU REALLY WANT
TO DELETE FILE ?

<YES/NO>

4.4.10.3
(Screen)
Display the Message while
Deleting a File.
---------- *FILES*----------

DELETING FILE
WAIT A MOMENT


4.5
SYSTEM MODE:

Press <4> Key from Menu to go the system

(Screen)

< # > Enter Choice and Proceed. --------------*SYSTEM*-----------
1.Lamps 2.Baseline
3.Diagnostics 4.RTC
5. Confirmation <#>


4.5.1
LAMPS:


Press <1> it is desired to changeover Lamp

4.5.1.1
(Screen)
< YES > Go to 4.5.1.2
< NO > Go to 4.5.1.3
--------------*Lamps*---------------
Wish to Change LAMP
Changeover : 380.0 nm
<YES/NO>

4.5.1.2
(Screen)

< # > Enter Wavelength between (360-400nm)
< ENT > Accept and Proceed.
< ESC > Go to 4.5.1.1
--------------*Lamps*---------------
Enter Changeover
nm
< #, ESC,ENT >

4.5.1.3
(Screen)
< 1 > To Change D2 Lamp Status
< 2 > To Change W Lamp Status
< ENT > Accept and Proceed.
--------------*Lamps*---------------
1. D
2
Lamp ON
2. W Lamp ON
< #, ESC,ENT >

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4 17


4.5.2
BASELINE:

Press <2> it is desired to select wavelength set
depending on the spectral range of use

4.5.2.1
(Screen)
< YES > Go to 4.5.2.2
< NO > Go to 4.5.2.3
------------*BASELINE*-----------
Do you Want to
Select Scan range
<
YES/NO>


4.5.2.2
(Screen)
< # > Enter Wavelength.
< ENT > Accept and Proceed.
< ESC > Go to 4.5.2.1

------------*BASELINE*-----------
Enter Scan Limits
SWL: 190.0 nm
EWL: nm < #, ENT
>

4.5.2.3 Displays the message and press ENT to
proceed.


------------*BASELINE*-----------
Remove cuvettes in
REF, SMP Holders <ENT>

4.5.2.4 Press <YES>to start Baseline Scan
Press <NO>Go to 4.5 After completing
Baseline Scan it displays Baseline Scan over
press any key and goes to 4.5 after Pres
<ENT>
------------*BASELINE*-----------
Start Baseline Scan?


<YES/NO>
4.5.3
DIAGNOSTICS:


Press <3> it is desired to enter Diagnostics
4.5.3.1
(Screen)
Type Password.
< ENT > Accept and Proceed.
< ESC > Go to 4.5
----------* DIAGNOSTICS*------
Enter PASSWORD:

ABC < ENT/ESC >

4.6
TIME SCAN:

Press <3> key, if is desired to select Time Scan Mode in Main Modes
4.6.1
(Screen)
< # > Wavelength Entry
< ENT > Accept and Proceed
< ESC > Go to Main Menu.
-----------TIME SCAN----------
ENTER WAVELENGTH:
XXX .X nm.
<# , ENT>


4.6.2
(Screen)
< # > Enter Scan Interval in Seconds.
< ENT > Accept and Proceed
< ESC > Go to 4.6.1
-----------TIME SCAN---------
ENTER SCAN INTERVAL:
Sec. (1.0 - 60)
<# , ENT>



4.6.3
(Screen)
< # > Enter Scan Period in Minutes.
< ENT > Accept and Proceed
< ESC > Go to 4.6.2
-----------TIME SCAN---------
ENTER SCAN PERIOD:
mnts.
(Max 16) <# , ENT>

SL 210 USERS MANUAL


4 18


4.6.4
(Screen)

<YES> AZ selected and Proceed to 4.6.5

-----------TIME SCAN---------
You Want Auto Zero
Option?

<YES/NO>

<NO> AZ not Selected and proceed to 4.6.8 -----------TIME SCAN---------
Place Reference in REF Holder

<ENT>

4.6.5
(Screen)

Display Message For a While. -----------TIME SCAN---------

Preparing to read
Please wait

4.6.6
(Screen)

< ENT > Accept Reference in REF and SMP
Cuvettes and Proceed.
< ESC > Go to 4.6.4
-----------TIME SCAN---------
Place REF & SMP
Cuvettes with BLANK
<ENT/ESC>

4.6.7
(Screen)

Display Message While
Reading REF CUVETTES.
-----------TIME SCAN---------
Scanning REF
Please Wait . . .

4.6.8
(Screen)
< ENT > Accept Sample in SMP
Cuvette and Proceed
-----------TIME SCAN---------
Place Sample in
SMP holder
<ENT>


4.6.9
(Screen)


You Find the Message on LCD While
Scanning Sample
-----------TIME SCAN---------
XXX.X nm TP P .P Min
Abs Y.YY.YY TI Q.Q Sec
Time RR: R sec <ESC>
Followed by
4.6.10
(Screen)

If we press <ESC> Time scan is being
interrupted.
-----------TIME SCAN---------
SCAN INTERRUPTED
1.VIEW 3.AUTOZERO
2.PRINT 4.MENU <#>
Time Scan VIEW:
Press <1> key in 4.6.10.9
4.6.10.1
(Screen)
If AZ Selected Find Message on LCD as
<NXT> Switch to ON or OFF
< ENT > Accept and Proceed.
< ESC > Go to 4.6.10.9
-----------TIME SCAN---------
Scanned Data
AZ <ON >
<NXT/ENT>

SL 210 USERS MANUAL


4 19


4.6.10.2
(Screen)

If AZ Not Selected Find
Message on LCD as.
-----------TIME SCAN---------
1. Scanned Data
2. Peak Abs
3. Parameters <#>
Press <1> key in 4.6.10.2

4.6.10.3
(Screen)


You Find the Message on LCD as
< ESC > Go to 4.6.10.2
-----------TIME SCAN---------
XXX.X nm TP P .P Min
Abs Y.YY.YY TI Q.Q Sec
Time RR: R <ESC>
Press <2> key in 4.6.10.2
4.6.10.4
(Screen)


< ENT > Proceed to Next Screen.
< Esc > Go to 4.6.10.2


-----------TIME SCAN---------
Maximum Abs: Y.YYYY
Max. Abs Time: Q. Q Sec
<ENT/ESC>

4.6.10.5
(Screen)

< ENT > Proceed to Previous Screen.
< Esc > Go to 4.6.10.2
-----------TIME SCAN---------
Minimum Abs: YYY. Y
Min. Abs Time: Q. Q Sec
<ENT/ESC>
Press <3> key in 4.6.10.2
4.6.10.6
(Screen)

You Find the Message on LCD as
< ESC > Go to 4.6.10.2
-----------TIME SCAN---------
Time interval: Q. Q Sec
Time Period: P. P Min
<ESC>
Press <2> key in 4.6.10.9 for Time Scan
PRINT

4.6.10.7
(Screen)


Press <NXT> to select Auto zero and
press <ENT>

-----------TIME SCAN---------
Select AZ status
To process DATA
AZ<ON> <NXT,ENT>
Followed by
4.6.10.8
(Screen)
Display Message on LCD While Printing after
proceed to 4.3.15.3.2
-----------TIME SCAN---------

Printing Under Progress
Please Wait . . .
Press <4 > key in 4.6.10.9
4.6.10.9
(Screen)
You Find the Message on LCD as DATA will
be Lost! OK <YES/NO>
< YES > Go to Main Menu.
< NO > Go to 4.6.10.9
-----------TIME SCAN---------
SCANNING OVER.
1.View 3. Auto zero
2.Print 4.Menu
<#>
4.7
STANDARDS PRESENTATION:

4.7.1
VIEW:

Press <1> key, if it is desired to view the data of
the Standards. If AZ Selected Go to 4.7.1.1
If AZ Not Selected Go to 4.7.1.2

SL 210 USERS MANUAL


4 20


4.7.1.1 < NXT > AZ Status on or off
< ENT > Accept and Proceed.
< ESC > Go to Menu.
--------*Quantitative*---------
Select AZ Status
To Process DATA
AZ < ON > < NXT, ENT >



If the Units of Concentration selected as ppm.

4.7.1.2
<? , ? > To change Standard number.
<#> Enter Sample number Directly
< ENT > Accept and Proceed.
< ESC > Go to Menu.
--------*Quantitative*---------
STD 1
ABS: X.XXX %E: X.X
CONC. X.XXXX ppm< # >
.
If the units of concentration selected as M
4.7.1.3
<? , ? > To change Standard number.
<#> Enter Sample number Directly
< ENT > Accept and Proceed.
< ESC > Go to Menu.
--------*Quantitative*---------
STD 1
ABS: X.XXX %E: X.X
CONC. XXXXEXX M



4.7.2 SAVE:

Press <4> key, if it is desired to save the data of
the Standards.


< ENT > Proceed
< ESC > Go to Menu.
--------*Quantitative*---------

ENTER NAME OF FILE

< ENT >

4.7.2.1


< # > Enter File Name
< ENT > Accept and Proceed.
< ESC > Go to Menu.
--------*Quantitative*---------

? ? ? ? Name of File

< ENT/ESC/#>

4.7.2.2 < # > Enter Quantitative Name of file
< ENT > Accept and Proceed.
< ESC > Go to Menu.
--------*Quantitative*---------
ABC

< ENT /ESC/ # >

4.7.2.3
< ENT > Accept and Proceed.
< ESC > Go to Menu.
--------*Quantitative*---------
Date :XX.XX.XXXX TUE
Time :XX.XX.XX .




4.7.2.4 Display Message While
Saving the File.
--------*Quantitative*---------
SAVING
WAIT A MOMEMT

SL 210 USERS MANUAL


4 21


Proceed to Main Menu.
4.7.2.5 If file name already exists
< ESC > Go to Menu.
--------*Quantitative*---------
FILE ALREADY EXIST
SAVE NOT POSSIBLE
<ESC>

4.7.2.6 If space is not sufficient to save the file
< ESC > Go to Menu.
--------*Quantitative*---------
Memory full Save
Not possible <ESC>


4.8
SAMPLES PRESENTATION

Press <2> key, if it is desired to measure the
samples.

< YES > AZ Selected
< NO > AZ Not Selected
Proceeds to 4.7.1.2 shows samples in place
of standard displayed in the screen.
--------*Quantitative*---------
You Want Auto Zero
option for samples?
< YES/NO >

4.9 MODES:
Press <5> key, to quit from the Data Presentation mode. Proceed to Main Menu.

4.9.1
PC Mode:
PC.0
<Screen>


If Communication is established then the
flow goes to PC.1 If Communication with
PC failed, flow goes to PC.2
------------*PC MODE*----------
Checking for PC
Please Wait
<ESC>


PC.1
<Screen>


Display Message While
Transferring Data
------------*PC MODE*----------

Transferring Base
Line Factors to PC


PC.2
<Screen>
< YES > Flow goes to PC.0
< NO > Flow goes to Main Menu.

------------*PC MODE*---------
PC Not Ready
Retry? <YES/NO>



IM 1
(Screen)


This message occurs when
the RAM is failed
ELICO * SL 210*
DOUBLE BEAM UV-VIS
RAM Failure
System Halted

IM 2
(Screen)

This message occurs when the home for
filter Drive is not detected within the
predefined no of Steps.
ELICO * SL 210*
DOUBLE BEAM UV-VIS
Filter Home Fail
System Halted

SL 210 USERS MANUAL


4 22


IM 3
(Screen)


This message occurs when the home for
grating Drive is not detected within the
predefined no of Steps.
ELICO * SL 210*
DOUBLE BEAM UV-VIS
Grating Home Fail
System Halted.


IM 4
(Screen)


This message occurs when the home for
Lamp Drive is not detected within the
predefined no of Steps.
ELICO * SL 210*
DOUBLE BEAM UV-VIS
Lamp Home Fail
System Halted.

Apart from these 6 options in Main Menu
we have another mode called Instant Mode

SL 210 USERS MANUAL


5 1


5. CHECKING THE PERFORMANCE

5.1 PRE-PERFORMANCE CHECK INSTRUCTIONS

Perform all the pre-operating instructions and keep REFERENCE empty & and
BENZENE VAPOUR (C
6
H
6
)-filled Cuvette as sample in the SAMPLE position
of the Cuvette holder.

NOTE

The Cuvettes should be thoroughly clean
and devoid of fingerprints.

CAUTION

Insert cuvettes vertically in the positions of
Cuvette holder 1 & 2 without imparting any
lateral motion to the cuvette holder, to avoid
misalignment of position of cuvettes. If any
lateral motion is imparted start the check test
over again.

5.2 SPECTRAL CALIBRATION

5.2.1 Select SPECTRUM MODE by performing operations of step 4.2.0.

5.2.2 Key-in Starting WL of 240 nm and Ending WL of 270 nm and scan the spectral
range selected performing operations of steps 4.2.1 to 4.2.9.1.2

If the Wavelength at the Peak Absorbance as read in the Graph Table is 253.9
0.5 nm, the spectral calibration of the instrument in UV spectral region is confirmed
to be proper.

5.3 PHOTOMETRIC PERFORMANCE

Select PHOTOMETRIC MODE by performing operations of step 4.3.0.

SL 210 USERS MANUAL


5 2


5.3.1 ABSORBANCE

5.3.1.1 Prepare 60 ppm K2Cr2O7 in 0.1N H2SO4. Select 257 nm as Wavelength WL-1 and
place 0.1N H
2
SO
4
as blank in REFERENCE and 60 ppm potassium dichromate as
test sample in SAMPLE and view or print ABSORBANCE of test sample at 257
nm by performing operations of steps 4.3.1 to 4.3.15.3.2
If the value of ABSORBANCE of Sample is 0.864 0.01, it is confimed that the
instrument is measuring ABSORBANCE correctly.

5.3.2 PERCENTAGE TRANSMISSION

5.3.2.1 As the value of PERCENTAGE TRANMISSION is derived form
ABSORBANCE itself, if the instrument is measuring ABSORBANCE correctly it
can be assumed that it measures PERCENTAGE TRANSMISSION also correctly.


5.3.3 CONCENTRATION

5.3.3.1 Prepare and keep handy potassium permanganate (KMnO
4
) solution of
Concentrations 10 ppm as STD-1, 20 ppm as STD-2, 30 ppm as STD-3, 15 ppm as
SMP-1. Place H2O as REFERENCE.

5.3.3.2 Select CONCENTRATION MODE, STANDARD METHOD of measurement, ppm
as CONCENTRATION UNITS and 545 nm as WAVELENGTH, by performing
operations of steps 4.4.1.7.1 to 4.4.1.8.11 measure and view concentration in ppm of
SMP-1.

5.3.3.3 If the value of Concentration displayed for the SMP-1 is 15 1.0 ppm, it is
confirmed that the instrument is measuring Concentration correctly.

SL 210 USERS MANUAL


6 1



6. TROUBLE SHOOTING

INDICATION POSSIBLE FAULT REMEDY

6.1


No display on
LCD Display.
1.


2.
Plug of power cord of the
instrument not inserted in
the power outlet.
Fuse at the power.
1.


2.
Insert Properly.


Check the fuse, if its
blow off, replace Fuse.

6.2 Prompt
Verify UV region
<Y/N>
1. Press YES for retry. 1. Switch OFF and wait
for more than 2
minutes & switch ON
again.
2. Press NO for moving
further.
2. If W Lamp is OK then
the problem may be
less energy or D2
Lamp Power supply
problem, correct it or
D2 Lamp life is over.
Lamp to be replaced.
If alignment problem,
re align.

6.3 Prompt

Check visible
region
Use (190.0nm to
379.9nm)
1. Visible region failed 1. W Lamp power supply
may be problem correct
it or Lamp filament
blow off. Lamp to be
replaced
If alignment problem,
re align



NOTE

For any other fault return the instrument to
your nearest ELICO Sales and Support Center.

SL 210 ANNEXURE-A


A 1

COLORIMETRY AND SPECTROPHOTOMETRY

1. INTRODUCTION

1.1 Colorimetry is concerned with the determination of the concentration of a substance
by measurement of relative absorption of light with respect to a known concentration
of the substance. In Colorimetry, white light is generally used as a light source, and
detections are usually made with photoelectric cell. In COLORIMETERS light
contained within a comparatively narrow range of wavelengths furnished by passing
white light through filters of colored glass, gelatin etc. is employed, transmitting only
limited spectral region. COLORIMETERS are also called FILTER
PHOTOMETERS.

1.2 In SPECTROPHOTOMETRY source of radiation used extends into the ultraviolet
region of the spectrum, from which definite wavelengths of radiation are chosen
possessing a bandwidth of less than 1 nm. In optical spectrometer measurements can
be made of the quantity of transmitted radiation. In SPECTROPHOTOMETER,
signal corresponding to the difference between the transmitted radiation of a reference
material and that of a sample at selected wavelengths are measured.

1.3 Colorimetric and Spectrophotometric methods provide a simple means for
determining minute quantities of substances.

2 THEORY OF COLORIMETRY & SPECTROPHOTOMETRY

2.1 When light (monochromatic or heterogeneous) falls on a homogeneous medium, a
portion of the incident light is reflected, a portion is absorbed within the medium, and
the remainder is transmitted. If the intensity of the incident light is expressed by Io,
that of the absorbed light by Ia, that of the transmitted light by It and that of the
reflected light by Ir then:

I
o
= I
a
+ I
t
+ I
r
(1)

Ir is usually eliminated by the use of comparison cells, as it is a mere 4% only,
rendering.

Io = Ia + It (2)

2.2 As per Lambert's Law when monochromatic light passes through a transparent
medium, intensity of the emitted light decreases exponentially as the thickness of the
absorbing medium increases arithmetically, expressed by

I
t
= Io.e
-kl (3)

Where Io is the intensity of the incident light falling upon an absorbing medium of
thickness l unit. It is the intensity of the transmitted light and k is a constant for the
wavelength and the absorbing medium used.

I
t
= I
o
10
-0.4343kl
= Io.10
-kl

(4)

SL 210 ANNEXURE-A


A 2

Where K = k / 2.3026 and is usually termed the ABSORPTION COEFFICIENT
generally defined as the reciprocal of the thickness (l cm) required to reduce the light
to 1/10 of its intensity.

The ratio It /Io is the fraction of the incident light transmitted by a thickness l unit of
the medium and is termed the TRANSMITTANCE T. Its reciprocal I
o
/I
t
is the
OPACITY and the ABSORBANCE A of the medium (formerly called the OPTICAL
DENSITY OD) is given by:

A = log (I
o
/ I
t
) (5)

2.3 As per Beer's law the intensity of a beam of monochromatic light decreases
exponentially as the concentration of the absorbing substance increases arithmetically.
This may be written in the form:

I
t
= Io.e
-k'c
= Io.10
-0.4343k'c
= Io.10
-K'c (6)

Where c is the concentration, and k' and K' are constants. Combining equation (5)
and (6), we have:

I
t
= Io = 10
-acl
or log (Io / It) = acl
(7)

2.4 This is the fundamental equation of COLORIMETRY and
SPECTROPHOTOMETRY, and is the principle of the famous BEER-LAMBERT
LAW. The value of A will clearly depend upon the method of expression of the
concentration. If c is expressed in mole L
-1
and l in centimeters then a is given
symbol and is called the MOLAR ABSORPTION COEFFICIENT or molar
absorptivity. The specific absorption coefficient s (sometimes termed absorbency
index) may be defined as the absorption per unit thickness (path length) and unit
concentration.

Thus there is a relationship between the ABSORBANCE A, the TRANSMITTANCE
T, and the molar absorption coefficient, since:

A = cl = log (Io / It) = log 1/T = - log T (8)

2.5 SPECTROPHOTOMETERS are often calibrated to read directly in ABSORBANCE
and also in PERCENTAGE TRANSMITTANCE. For colorimetric measurements Io
is usually understood as the intensity of the light transmitted by the pure solvent, or
the intensity of the light entering the solution. I
t
is the intensity of the light emerging
from the solution or transmitted by the solution. It will be noted that:

The MOLAR ABSORPTION COEFFICIENT is the specific absorption coefficient
for a concentration of I mole L
-1
and a path length of 1 cm.

= A / cl (9)

SL 210 ANNEXURE-A


A 3

2.6 If two solutions of a colored substance with concentrations of c1 and c2 are placed
in an instrument in which the thickness of the layers can be altered and measured
easily which also allows a comparison of the transmitted light and both layers have
the same colour intensity:

It
1
= Io.10
-
l1
C1
= I
t
2 = Io.10
-

l2c2 (10)

Where l
1
and l
2
are the lengths of the columns of solutions with concentrations c
1
and
c
2
respectively when the system is optically balanced. Under these conditions, as per
Beer's Law:

l
1
c
1
= l
2
c
2
(11)

2.7 If the concentration c1 is known concentration Cx of an unknown solution can be
calculated from:

Cx = [log (Io / It)] / l (12)

When MOLAR ABSORBTION COEFFICIENT, which depends on wavelengths of
incident light, temperature and solvent, employed is known and matched cells are
used (i.e.c
1
is constant).

c log (I
o
/ I
t
) or c log (1/T) or C A (13)

Hence, by plotting A (or log (1/T)), as ordinate, against concentration as abscissa, a
straight line will be obtained and this will pass through the point c=0,A=0 (T=100%).
This calibration line may then be used to determine unknown concentrations of
solutions of the same. Material after measurement of absorbances.

NOTE

THIS THEORY OF COLORIMETRY AND
SPECTROPHOTOMETRY IS MEANT ONLY AS
AN INTRODUCTION TO THE SUBJECT. THIS
IS NEITHER EXHAUSTIVE NOR COMPLETE.


Reference:
G.H. JEFFERY, J.BASSETT, J.MENDHAM and R.C.DENNEY Vogel's Text book of
Quantitative Chemical Analysis, 5th Edition LONGMAN SCIENTIFIC & TECHNICAL,
LONGMAN GROUP UK LTD., HARLOW, 1989.

SL 210 ANNEXURE-C


C-1

DOUBLE BEAM
UV-VIS SPECTROPHOTOMETER
SL 210

SALIENT FEATURES AND BENEFITS


Smallest form factor double beam spectrophotometer with a high-resolution
bandwidth

Comes with automatic wavelength calibration, programmable wavelength for lamp
changeover

Lamp selection enables conserving the life of the two lamps, deuterium and
tungsten

Menu driven microprocessor based software makes the stand-alone unit user
friendly, the feature enhanced by the soft touch keypad and display.

Stand alone unit offers an advanced technology with data processing features for
scanning discrete wavelength, concentration determination by standards and -
factor method, time scan with minimum time interval of 1.0 sec, auto-zero facility,
self check and self diagnostic modes.

Data in the form of graph and table can be printed directly onto a printer.

PC compatible through USB

Multitasking user friendly PC software provides the analysis to run all the features
available in the stand alone unit and additionally has techniques to view, analyze
and display upto 4
th
order derivative spectra, overlay of maximum ten spectra, peak
picking facilities, % concentration mode, auto-zero facility, spectra zooming,
smoothening, data saving and printer

Software is 21 CFR Part 11 compliant with password method protection; Instrument
Log Book etc. cGLP compliance is addressed.

SL 210 ANNEXURE-C


C-2

SPECIFICATIONS

PARAMETERS

SPECTRAL
Range 190 to 1100 nm
Bandwidth 1.8nm
Accuracy 0.5 nm
Repeatability 0.2nm
Readability 0.1nm

PHOTOMETRIC
System Double beam optics
Range 3.000Abs
Accuracy 0.005Abs at 1.0Abs
0.010Abs at 1.5Abs
Repeatability 0.002Abs at 1.0Abs
Stability (Baseline) 0.003Abs/hr. after 2hour warm-up
Stray Light < 0.05%T at 220nm with Nal 10g/L
Readability %T 0.01
Abs 0.0001
LIGHT SOURCE Deuterium Lamp (D
2
) & Tungsten (W) Halogen Lamp
MONOCHROMATOR Concave holographic grating with 1200 lines/mm
DETECTOR Photo Diode
CONTROL Microprocessor and Microcontroller (Computer-Optional)

DATA PRESENTATION
Display 4 line 20 character Dot matrix backlight LCD module
Hard Copy On Printer (Centronics)
DATA PROCESSING
Microprocessor Mode Spectrum, Discrete , Concentration, -factor, Time Scan
and Auto zero
PC Mode Spectrum, Overlay, Time Scan, Discrete , Ratiometric,
Concentration, -factor, Upto 4
th
order derivative, Peak
Picking, Spectra Zooming, Data saving, Retrieving and
Printing
PC CONNECTIVITY USB Interface
SAMPLE ATTACHMENT
Standard Fixed 10mm path holders for Reference & Sample
Optional Fixed multiple cell holders for Reference & Sample
accommodating 10 to 100mm cuvettes
Fixed cell holder for Reference & 6 position motorized
10mm path cell holder for sample
Fixed cell holder to accommodate microcuvettes

SL 210 ANNEXURE-C


C-3

POWER REQUIREMENT 90-260 V, (50-60) Hz, 1, Max.170VA
PHYSICAL DIMENSIONS
Size (WXDXH) 500 x 375 x 180
Weight in kg Approx. 15
ACCESSORIES SUPPLIED
With Instrument 10mm path length cuvettes
At extra cost PC, UV-VIS spectra Treats

Software, Multiple path length

cuvette holder, 10, 20, 40, 50 & 100mm path length cuvettes
Microcuvettes and Printer



APPLICATIONS

This Instrument finds place for any spectrophotometric or colorimetric
measurement where applications require very high-resolution bandwidth and cGLP
compliance is mandatory.

Micronutrients like N, P, K, S, Ca, Mg, Zn, Cu, B, Mo, etc., in Agricultural soil, Plants
etc.

Organic compounds in Biological matter

Glucose, Fructose, Carbohydrates, Proteins etc., in Foods.

Edible dyes, alcohols etc., in Beverages

Purity of constituents in Pharmaceuticals

Toxic elements like Cadmium, Lead, Mercury and Arsenic etc., in Effluents.

Constituents in compositions used in Metallurgy, Fertilizer, Pesticide, Chemical, Petro
Chemical, Steel, Cement, Glass & Other Industries.

Life science applications

SL 210 ANNEXURE-E


E 1


CARE & MAINTENANCE OF CELLS
(CUVETTES OR PRECISION OPTICAL CELLS)

1. CARE & MAINTENANCE OF CELLS

1.1 GENERAL

Use cells-appropriate to the spectral region of interest & application envisaged and
free from scratches, etch marks & other physical defects arising due to improper
handling - in perfectly clean condition, to make full use of the accuracy and sensitivity
of the electro-optical analytical instruments in which they are being employed.

1.2 CHOICE OF CELLS

For measurements in the visible region use cells of special optical glass.
For measurements in the Ultra-Violet region of less than 360 nm wavelength use
quartz cells.

For measurements in the Ultra-Violet region of less than 220 nm wavelength, only
certain varieties of silica cells are satisfactory, such as those marketed under trade
names Spectrosil, Supersil, Ultrasil, supersil, etc.

1.3 CARE OF CELLS DURING USE

1.3.1 Always clean the cells thoroughly and rinse at least once with a portion of the sample,
before filling the sample, for use.

1.3.2 Always wipe the exposed surfaces of the cells dry and free from lint & finger prints,
using lintless cloth or soft tissue paper.

1.3.3 Any glass (including vitreous silica) surface can rapidly absorb contamination. Gross
contamination can occur from solvent evaporation, dust or grease. Such films or dust
particles decrease the transmission and may contaminate the sample. For example,
cells left standing on a laboratory bench for one hour show a drop in transmission up
to 0.5%. It is important, therefore, to clean cells immediately after use and prior to
use.

1.3.4 While testing proteins or similar organic substances, rinse cells frequently to prevent
sample build-up. Never allow such samples to dry within the cells.

1.3.5 Immediately after use empty and rinse the cells with the solvent, clean with a mild
cleaning agent and store in distilled water, for short time storage.

Do not leave solutions, particularly strong alkalis, in the cells for periods longer
than necessary.

SL 210 ANNEXURE-E


E 2

1.3.6 Always handle wet cells using tongs or tweezers, the ends of which are covered by
soft, inert material like rubber or PTFE and grasp the cells only on the ground non-
optical surfaces. The use of clean cotton gloves when handling dry cells prevents
perspiration from fingers spreading to the polished faces of the cells.

NOTE
Ensure no bubbles cling to the inner surfaces of
the cells. Finger marks/ prints absorb very
strongly light in UV region.

1.3.7 Never use a brush or any instrument, which might scratch the optical surfaces.
Scratches and etching do not necessarily render a cell useless but can reduce
transmission and cause light scattering. Always remove solid contamination by wet
cleaning method like ultrasonic agitation and never by mechanical means.

1.3.8 Keep the cells in their original cases, with their etched surfaces on the top, when not
in use, for long time.

1.4 CLEANING METHODS

1.4.1 Cleaning procedures vary with the type of cell to be cleaned, accuracy required and
type of contamination occurring from previous use. Use a cleaning method simplest
of the ones indicated below appropriate to the application.

1.4.2 A number of cleaning methods are listed in increasing order of severity. Whichever
cleaning procedure is adopted, it is important that, once cleaning has commenced, do
not allow the surfaces to dry until the cleaning procedure has been completed.

1.4.3 Mild cleaning methods

1.4.3.1 Method-1

Boil the cells for 15 minutes in a 2% solution of a detergent and follow it with
adequate rinsing in distilled water and Ethanol before drying.

1.4.3.2 Method-2

Take 1 gram of Potassium Dichromate, add a little distilled water and very slowly add
approximately 100 ml of concentrated H
2
SO
4
. Keep the cells in this acidic solution
for a maximum period of 12 hours. Wash the cells thoroughly with distilled water
before use.

NOTE
Cells may get damaged if excess Fluoride is present
in the water that is added to Potassium Dichromate.

SL 210 ANNEXURE-E


E 3

1.4.3.3 Method - 3

Immerse the cells and leave overnight in freshly prepared Chromic Acid and rinse
thoroughly with distilled water before use.

NOTE
Potassium Dichromate dissolved in concentrated
Nitric Acid is preferred to a Chromic - Sulphuric
mixture because of its greater oxidizing capacity
and mobility.

1.4.3.4 Method-4
Add 30 ml concentrated Hydrochloric Acid to 10 ml concentrated Nitric Acid (Acqua
Regia). Fill the cells with the mixture and allow to stand for 5 to 10 minutes.
Thoroughly wash the cells with distilled water before use. Repeat the procedure if
necessary.

NOTE
These methods are generally adequate for cells,
which are to be used in the visible and infrared
spectral regions. Cells, which are to be used in the
ultra-violet region, may not be adequately cleaned
by these methods.

1.4.4 General method for cleaning cells used in the Ultra-Violet region

1.4.4.1 Immerse cells in concentrated Nitric Acid and agitate either ultrasonically or by
boiling for 10 to 15 minutes. Rinse repeatedly with distilled water followed by rinse
in absolute Ethanol and dry.

NOTE
Correct application of this method should give
transmission variations of less than 5%.

1.4.4.2 Cells cleaned by this method normally require matching to give sets of cells with a
transmission variation of less than 1.5% at 240 nm.

NOTE
B.S. 3875: 1965 requires that matched type 1
cells show a transmission variation of less than
1.5% at 240 nm.
Cells cleaned by this procedure may vary in
transmission due to different degrees of
cleanliness but not because of material variations.

SL 210 ANNEXURE-E


E 4

1.4.5 Method for cleaning cells to ensure reproducible Ultra-Violet transmission

1.4.5.1 Synthetic fused silica cells like Spectrosil do not exhibit the variations in
transmission shown by other forms of vitreous silica. Such cells will match
automatically, without selection.

NOTE
This inherent uniformity of transmission will be
lost if cells with transmitting plates of
Spectrosil are not scrupulously clean.

1.4.5.2 Method

1.4.5.2.1 Remove all traces of liquid previously used in the cells, wash in water, agitate in
concentrated Nitric Acid (boiling or ultrasonic bath) and subsequently rinse in
distilled water.

1.4.5.2.2 Immerse the cells in hot (80 to 90
o
C) saturated solution of Tri-Sodium
Orthophosphate for 10 minutes, with repeated decantation.

1.4.5.2.3 Rinse in several changes of hot distilled water.

1.4.5.2.4 Immerse, with repeated decantation, in boiling distilled water for 10 minutes.

1.4.5.2.5 Rinse in cool reagent grade Ethanol.

1.4.5.2.6 Rinse thoroughly, at least twice, in hot, freshly distilled Ethanol. Allow to dry in
clean dust-free air.

NOTE
Vitreous silica or inert plastic (e.g. PTFE) should
be used for operation of steps 1.4.5.2.4 onwards
since glass vessels may introduce contamination.

This is only one of a number of possible methods and is not recommended for
repeated use since slight etching of the transmitting plates can occur with
prolonged immersions. However it demonstrates effectively the results of
efficient cleaning.



This method is capable of cleaning Spectrosil cells to a degree such that the
variation in transmission at 240 nm will be less than 1%.

SL 210 ANNEXURE-E


E 5

1.4.6 Special methods

1.4.6.1 Method - 1

If stubborn organic contamination is present and it is not possible to obtain the
required level of transmission by operation of steps of 1.4.5, use hot Perchloric Acid
instead of Orthophosphate in 1.4.5.2.2.

CAUTION
Perchloric acid is a dangerous liquid and
due care should be exercised in its use.

1.4.6.2 Method - 2

1.4.6.2.1 Excellent results can be obtained by cleaning the transmitting plates of demountable
cells using the Ethanol-Nitric acid reaction. Transmission variations of less than
0.5% at 240 bn can be achieved for Spectrosil plates using this method carried
out in a fume cupboard.

1.4.6.2.2 Place the plates in a shallow, curved-bottomed Vitreosil or porcelain dish with not
more than 0.5 ml, Ethanol in a fume cupboard. Add about five times this volume of
cold concentrated Nitric Acid and cover the dish immediately with a large, inverted
beaker. Close the fume cupboard. After the reaction has subsided carry out the
recommended rinsing and drying procedure.

CAUTION
This method is as DANGEROUS as it is
effective. Any Ethanol Nitric Acid mixture
reacts violently, immediately or after a delay,
producing a large volume of noxious fumes.
Therefore it should only be used if the dangers
involved are fully understood.

NOTE
To obtain the maximum benefit from any cleaning
method, the final stages of rinsing and drying are
critical.

Copious rinsing in distilled water is recommended followed by a final rinse in
absolute Ethanol or other pure readily volatile and miscible with water solvent,
before drying. Where possible, drying should be accelerated by gentle heat or
vacuum.

Drying the cells with a jet of air is not recommended but may be used if adequate
precautions are taken (e.g. using appropriate filters, drying agents, etc.).

SL 210 ANNEXURE-E


E 6

CAUTION
These cleaning methods can safely be applied
to cells of all-fused construction. Cemented
cells and those incorporating intermediate
glasses in their construction may be damaged
and may even disintegrate using some of the
processes described.

1.4.7 Checking the cleanliness of cells
A clean cell filled with distilled water should have the following absorbance
percentage Transmission values relative to air.

Cell type Wavelength (nm) Absorbance
(Abs.)
%T
Optical Glass 650 < 0.035 > 90
Silica 240 < 0.093 > 80

























SL 210 ANNEXURE-F
F-1

















NOTE

DISPOSE THE MATERIAL IN
THE SAFE ENVIRONMENT
AFTER USE OR HANDOVER TO
OUR NEAREST SALES OFFICES





INSTRUMENT WARRANTY CARD

Instrument Model Sl.No.
Customers Ref. No. Date
Elico Delivery Note No. Date

PLEASE PRINT


This Instrument is warranted against defects in material and workmanship for a
period of one year from the date of shipment.
For ELICO LIMITED


1. Please fill in, separate at perforation and mail the self addressed Instrument
Registration Card within 21 days of the date of Delivery Note.

2. Please inform any change in your address.

3. This warranty shall not apply to fuses, lamps, batteries, glass & fragile items
and to defects resulting from improper location, installation, operation or
battery discharge.

4. This warranty becomes void if the Instrument is serviced by Agencies other
than ELICO Sales & Support Centres or resold by the original customer.

5. Suggestions for improvement of the instrument and Documentation provided
will be highly appreciated.







INSTRUMENT WARRANTY CARD

Instrument: Model: Sl.No.:
Customers Ref. No.: Date:
Elico Delivery Note No.: Date:


Signature:

Name:
Customer Address:


Date:

Signature:

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Date:
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ELICO Limited
B-90, A.P.I.E., Sanathnagar
Industrial Estate Extension
HYDERABAD 500 018. India



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CERTIFICATE
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time of shipment from the factory after due to calibration.

WARRANTY
This instrument is warranted against defects in material and workmanship for a
period of ONE YEAR from the date of shipment (except for those components, whose
warranty shall be as indicated specifically in the manual). During the warranty period
ELICO LIMITED will at its option, either repair or replace products which prove to be
defective.
For warranty service or repair, this instrument must be returned to the nearest Sales
and Support Centre of ELICO LIMITED by person or by Passenger Train or Air,
packed in a manner similar to which the product was received, insured and shipping
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ELICO Limited
B-90, A.P.I.E., Sanathnagar, Hyderabad 500 018, AP., India.
Tel: +91-40-4445 1202, 2377 0135 Fax: +91-40-2377 1639
E-mail: elico@elico.co URL http://www.elico.co