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B.K.K.K. Jinadasa
GS/M.Sc./FOOD /3608/08
1/16/2010
Determination of Fat
2.0 Introduction
Most fat in the diet is in the form of triglyceride (three fatty acids esterified to a glycerol
molecule backbone). There are also non-glyceride components such as sterols, e.g. cholesterol.
Fat is used to describe those lipids that are solid at the specified temperature.
Lipids have at least three important functions in foods; culinary, physiological and nutritional.
Lipids have the ability to carry odours and flavours and contribute to the palatability of meats, to
the tenderness of baked products and to the richness and texture of ice cream. Dietary lipids
represent the most compact chemical energy to man. They contain twice the caloric value of an
equivalent of sugar.
Lipids are usually defined as food components that are insoluble in water and that are soluble in
organic solvents. Solvents used to extract lipids include ether, petroleum ether, acetone,
chloroform, benzene, alcohols and water saturated butanol. Successful extraction requires that
bonds between lipids and other compounds be broken so that the lipids are freed and solubilized.
Generally such solubility is attained when polarities of the lipid and the solvent are similar.
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Determination of Fat
2.1.1. Materials
Sample – sausages
2.1.2. Procedure
5 g of finely ground sample was taken in a motor and anhydrous sodium sulphate of twice the
weight of the sample was added into it. Then the mixture was ground until a free flowing powder
was obtained. Then the powder was transferred to a thimble and sealed the end. Extraction
thimble with the sample was placed in the soxhelt apparatus and fixed a previously dried and
weighed round bottom flask. 200 mL of extracting solvent (petroleum ether) was added to the
flask containing pumice chips. Then the Flask and the condenser were connected to the soxhelt
extractor.
Sample was allowed to reflux for about five hours. After the extraction flask was removed from
the apparatus and kept in the water bath and then in the oven. Then the flask was cooled and
weight was taken.
= 9.21 %
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Determination of Fat
2.1.4. Discussion
Soxhlet extraction is one of the most commonly used methods for determination of lipids in
dried foods. This is mainly because it is fairly simple to use. The main disadvantages of the
technique are that a relatively dry sample is needed (to allow the solvent to penetrate), it is
destructive, and it is time consuming.
2.2.1. Materials
2.2.2. Procedure
Three beakers were taken and 2 g of sample was placed in each beaker. Then 2 mL of 95% ethyl
alcohol and 10 mL of prepared HCl (by mixing 25 mL of Conc. HCl and 11 ml of water) were
added to each beaker and thoroughly mixed. Then the beakers were placed in a water bath (70ºC
- 80 ºC) with stirring them frequently for about 30 - 40 minutes till the contents of beakers turn
blackish brown in colour.
Beakers were taken out from the water bath and cooled to room temperature. Then 10 mL of
ethyl alcohol was added and contents were transferred to three majonnier flasks.
Then each beaker was washed with 25 mL of diethyl ether in three portions and added to
respective flasks. All the flasks were stoppered and shook vigorously for about 1 minute.
25 mL of PET ether was added to each flask and shook for 1 minute.
Then the flasks were allowed to stand undisturbed until the upper ether layer is clear. Upper
ether layers were taken into clean dried weighed flasks and dried them in a water bath at 80 ºC
until a constant weight obtained.
A B C
Weight of flask(g) 50.9437 44.2183 56.7882
Weight of sample(g) 2.0035 2.0000 2.0015
Weight of flask + extracted fat 51.2765 44.5494 57.1200
(after drying) (g)
Total fat 16.61 16.56 16.58
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Determination of Fat
= 16.58 %
2.2.4. Discussion
In total fat determination both free fat and bound fat are taken into account. Here the bound
lipids can be made free by dissolving the food sample in polar solvents. Dissolution of food can
be achieved by acid. In this method the food material is heated in a water bath with hydrochloric
acid in the sample to break down the protein, and fat separates as a layer on the top of the acid
liquid. The protein dissolves in the acid and the separated fat can be extracted by shaking at least
three times with diethyl ether or diethyl ether and light petroleum mixture.
Shape of the majonnier flask aids in settling down all the coagulated protein at the bottom
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