BIOSAFETY

 Biosafety

BSL 1
Not known to consequently cause disease in
healthy human adults
Pose
minimal
hazards
under
ordinary
conditions of handling



GMT )Good Microbiological
(Technique

BSC Biological
Safety Cabinet


Standard Practices
Use Mechanical Pipetting
devices
No Eating, Drinking, Smoking
in Lab
Minimize splashes and
aerosols
Decontaminate work surfaces
Safe handling of sharps
Wash Hands before leaving lab
Protective Clothing
Lab Coat
Gloves
Eye Protection

Laboratory practices
Standard microbiological practices
on an open lab bench or table.
Safety equipment
Personal protective equipment,
(lab coats, gloves, eye protection)
are worn as needed.
Facility construction
A sink must be available for hand
washing.
The lab should have doors to
separate the working space with
the rest of the facility.



Low
Community Risk


.
BSL 2
well-characterized agents not known to cause
disease in healthy adult humans, and of minimal
potential hazard to laboratory personnel and the
environment
Examples: Measles Virus, Salmonella, Hepatitis B
Virus


GMT

biohazard sign

.BSC









)







work is done with indigenous or exotic agents which
may cause serious or potentially lethal disease as a
result of exposure by the inhalation route.
E.g.
Mycobacterium Tuberculosis
Hanta virus
SARS


GMT



negative


pressure

.
Examples
Ebola Virus
Monkey B Virus
Marburg Virus

GMT






.

III


.

Risk group classification

Risk
Group

Individual risk

Community risk

no, low

no, low

moderate

low

high

low

high

high


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pH .

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%70

15