RAMAN ACCESSORIES

Raman is an exceptionally versatile molecular spectroscopy

technique that offers solutions for many of spectroscopy’s
Contents
most challenging problems today. Raman offers solutions for
FT-IR Raman 108
both micro and macro sampling, both organics and inorganics, Accessory
both qualitative and quantitative applications, and can be Macro Sample 109
readily applied to the analysis of solids, liquids, and contained Handling Tools

gases. Raman spectroscopy is used today for a variety of Spinning Vial Holder 110

applications in the material science, pharmaceutical, chemical, Spinning Cup 110

Sampling Accessory
polymer, semiconductor, and forensic fields.
Bottle Holder 111
Sampling Accessory

Carousel Autosampler 111

MicroStage 112
FT-Raman Microscope

Well-plate Adapter 113

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Provides
information Nicolet NXR FT-Raman Accessory for Research-level
on infrared
inactive and
weak bonds
Nicolet FT-IR Instruments
Solids The Nicolet NXR FT-Raman module is an
Liquids accessory that can expand the capability
of the Nicolet research-level FT-IR instru-
G
ments to include FT-Raman. Raman is a
Microsampling
complementary technique to infrared and
often provides additional information
about samples. Thus adding the FT-Raman
module to a FT-IR instrument can greatly
increase the amount of information that
the instrument can provide about the
sample. Raman activity arises from
molecular bonds that have a very weak
natural dipole moment – typically highly
symmetric bonds. In contrast, infrared
activity arises from molecular bonds that
have a strong natural dipole moment –
typically highly polar bonds. As a result,
infrared is generally an excellent tool for
characterizing the end groups in a molecule
and Raman typically provides better char-
Applications Compatibility
acterization of the molecular backbone.
• Organics and inorganics • Nicolet Magna series
• Crystalline polymorphs • Nicolet Nexus series
• Raw material identification • Nicolet X700 Series
• Particulate contamination analysis * Note that some instrument configurations may
• Quantitative analysis require additional component upgrades

Features
• Macro sampling options
• Micro sampling options
• Controlled environment sampling options
Raman Advantages
Raman also offers a number of sampling Product Configuration Part Number
advantages that may make Raman a 50 cm-1 option for Research configuration 470-220500
preferred technique for specific samples. Base FT-Raman module for Research configuration 912A0541
• Samples analyzed neat Base FT-Raman module for Routine analysis configuration 912A0542
• Sampling performed through glass and Call for complete configuration details
thin plastic
• Toxic materials can be analyzed
without ever opening the container
• Insensitive to atmospheric H2O and CO2
• Routine collection down to 50 cm-1
• Less sensitive to molecular interactions –
spectra subtract better and peaks
are sharper

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 RAMAN ACCESSORIES

Macro Sample Handling Tools for Raman Spectroscopy Versatile

macro
sampling
The ease of sample handling is one of the Solids
major benefits of Raman spectroscopy. Liquids
This group of sample holders provides G
convenient means to hold and position
M
samples in the Nicolet FT-Raman and dis-
persive Raman spectrometers. All holders
are designed to fit the motorized sampling
position stages found in the Nicolet Raman
spectrometers. Where appropriate, the
sample holders are gold coated for more
efficient collection of Raman scattered light.

180° Sampling Accessories

These versatile sampling accessories are
the traditional favorites for Raman sam-
pling. These accessories collect the emitted
Raman scattering from the same surface
that is excited by the laser and generally
provides the highest throughput of any Powder Holder and Finger Press Film Holder
of the Raman sampling accessories.
Convenient gold-coated sample holder for Holder for relatively thin solids such as
The accessories come standard with a
small quantities of loose powders. The polymer films (up to 2 mm thick). The film
gold-plated NMR tube holder and may be
mount holds the sample at the correct is easily clipped between the holder and
used with any of the specialized sampling
height in the sample compartment. The the magnetic mount.
mounts mentioned on this page.
finger press is used for loading and
compressing powders in the powder Solids Holder
90° Refractive
Sampling Accessory holder. Spare holders are available to A spring action c-clamp style for holding
increase lab efficiency. many sizes and shapes of extended solid
An alternative to the 180° Reflective
samples, packaged products, or small vials.
accessory for FT-Raman, this accessory Adjustable Vial Holder
generally does not offer sensitivity as
Convenient holder for circular vials and
high as the 180° accessory but is useful
rectangular cuvettes ranging from 7 mm
in conjunction with the 180° accessory
to 15 mm in diameter. Vials are held
for polarization experiments where more
securely in place for sampling with a
than one collection angle is needed. It is
finger-tightened screw clamp. The holder
compatible with the vial holder and the
leaves the sample visible from the front
solids holder.
for excitation and collection and on one
Raman Sample Holder Kit side for sampling with the alternate 90°
sampling accessory.
The kit includes the following sample
holders in a storage case: capillary holder,
Product Configuration Part Number
powder holder and finger press, film holder,
180° Reflective Sampling Accessory (FT-Raman) 840-050500
and solids holder.
180° Refractive Sampling Accessory (Dispersive Raman) 840-081900
Sampling Tubes and Tube Holders 90° Refractive Sampling Accessory (FT-Raman) 840-046300
Raman Sample Holder Kit 840-043300
This gold-coated sample holder is ideal
NMR Tube Holder (gold plated) 071-750703
for holding standard NMR diameter tubes.
NMR Sample Tubes, 10 699-048000
The sample tubes have very low background
Capillary Tube Holder 071-749303
and are fluorescence free in FT-Raman
Powder Holder and Mount 470-191300
experiments using 1064 excitation. The
Replacement Powder Holder nut 078-726401
gold-coated capillary tube holder is an
Finger Press 470-191400
excellent alternative when sample
Film Holder and Mount 470-191500
quantities are limited. It accommodates
Spare Film Holder nut 078-726501
standard (~1.6 mm O.D.) diameter melting
Solids Holder (spring loaded) 470-179300
point tubes.
Adjustable Vial Holder (7 – 15 mm) 470-213400

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Reproducible
measurements Spinning Vial Holder
of powder
mixtures
Sometimes it can be difficult to obtain a representative spectrum of mixtures of powders
Solids or other heterogeneous samples because the small area of the sample that is actually
Liquids measured may not represent the whole. The Spinning Vial Holder provides a solution to
G this problem by spinning the sample within a vial as it is measured to expose a larger
M area of the sample to the excitation laser. Spinning the sample greatly improves the
reproducibility of the measurement when dealing with heterogeneous samples. Samples
are held in place with a spring-loaded clamping mechanism. Contact with the vial is by
a rubber wheel that spins the vials as they are sampled.

Applications
• Mixtures of powders Product Configuration Part Number
• Emulsions Spinning Vial Holder 869-118600

Features
• Accommodates vials up to 15 mm in
diameter
• Requires 180° sampling accessory

Figure 1: Replicates of sucrose/dextrose mixture, Figure 2: Replicates of sucrose/dextrose mixture,

static sampling with spinning

Reproducible
measurements Spinning Cup Sampling Accessory
of powders and
other solids
Sometimes it can be difficult to obtain a representative spectrum of mixtures of powders
Solids
or other heterogeneous samples because the small area of the sample that is actually
L measured may not represent the whole. The Spinning Cup accessory provides a solution
G to this problem by spinning the sample within a cup as it is measured to expose a larger
M area of the sample to the excitation laser. Spinning the sample greatly improves the
reproducibility of the measurement when dealing with heterogeneous samples. Samples
are placed in a gold-plated cup and measured from above.

Applications
• Mixtures of powders
• Natural products
Product Configuration Part Number
Features Spinning Cup Sampling Accessory Call for ordering information
• Gold-plated sampling cups

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 RAMAN ACCESSORIES

Bottle Holder Sampling Accessory Sample raw

materials with-
out opening
the bottles
The bottle holder allows sampling of liquids and solids directly in the bottle. The sample
is measured by simply laying the bottle on its side on the accessory orientated such that Solids

labeling does not obscure the sampling point. The accessory works best with clear glass Liquids
bottles. The bottle glass reduces throughput somewhat compared to sampling in an G
NMR tube, but ease of sampling is unparalled as samples can be measured without M
ever opening the bottle.

Applications
• Raw Material Identification
• Screening of Unknown Materials

Features Product Configuration Part Number

• Easy sampling of materials in clear Bottle Holder Sampling Accessory 840-050200

glass bottles Adapter plate for Nicolet Almega systems 002-857400
• Compatible with Nicolet FT and
Dispersive Raman systems
• Not recommended for 976 nm excitation
configurations because of strong glass
fluorescence at that wavelength

Automated
Carousel Autosampler sampling
of liquids
and solids
Labs with high sample volumes will benefit from automated analysis. The carousel
Solids
autosampler allows a series of samples to be prepared and loaded in the instrument
and then measured unattended – freeing the analyst up to work on other tasks. Sample Liquids
information can be entered in spreadsheet format and macros can be linked in to G
automatically perform more sophisticated data analysis. M

Applications
• Automated analysis of liquids and
powders
• Raw material analysis
• Finished product analysis
• Screening applications

Features Product Configuration Part Number

• Samples loaded into interchangeable Carousel Autosampler Call for ordering information
carousels
• Carousels hold 16 NMR tubes or 8 vials
(13 mm diameter)
• Custom carousels for different diameter
vials available on request
• Vial spinning functionality built in
for reproducible sampling of powder
mixtures
• Compatible with Nicolet FT and
dispersive Raman systems

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Point and
click micro MicroStage FT-Raman Microscope
and macro
sampling
The MicroStage FT-Raman microscope is a unique product providing both micro and
Solids macro sampling capabilities in a single compact accessory. The microscope incorporates
Liquids an integrated video microscopy capability making it easy to obtain the correct focus for
G optimized Raman performance and target isolated sample particles. The motorized x-y-z
Microsampling manipulator is fully controlled from OMNIC spectroscopy software. Optional µView
software allows you to select the analysis point by simply clicking on the video image.
The ViewStage is also supported by the Atlµs mapping software package and the Array
Automation software.

Applications
• Macro sampling of bulk materials and
solutions in sample cups
• Micro sampling of fibers, crystals, or The ViewStage, the predecessor to the
MicroStage, was described by R&D
defects down to 50 microns Magazine as “revolutionizing spectroscopy”
• Automated sampling of 96-well and awarded the 2002 R&D 100 award.
Microtiter™ plates
• Chemical mapping of sample surfaces
The ease of sampling with the MicroStage FT-Raman microscope accessory is
Features demonstrated below:
• Built-in color CCD video system Step 1 – Place the sample on a microscope slide or sampling plate and load in instrument.
• x-y-z motorized stage and controller
with 8.25 cm travel x, 12.4 cm travel y,
1.3 cm travel z
• Horizontal stage holds sample cups
microscope slides, and microtiter
well plates
• Supports sampling through glass
coverslips
• NTSC composite output via BNC
connector
• High throughput free-space optics
• Surface profiling yields chemical
images
Step 2 – Select the area of interest with Step 3 – Measure spectrum and
• Automated analyses of array-based
samples the mouse on the screen display. analyze results
• Exceptional ease-of-use
• Built-in beam expander for sampling
larger areas

Compatibility
• 960 FT-Raman spectrometer
• Nicolet FT-Raman modules
• Nicolet NXR FT-Raman modules

Product Configuration Part Number

Call Thermo for complete ordering information
Base MicroStage FT-Raman microscope 840-157200
Array Automation software 834-032700
OMNIC Atlµs software 834-035301
µView software 834-035401

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 RAMAN ACCESSORIES

Well-plate Adapter for the Nicolet Almega Automated

well-plate
sampling
Dispersive Raman System Solids

Microtiter well-plates have become the standard medium for many analysis platforms Liquids

today. This adapter allows standard well-plates to be loaded onto the Nicolet Almega G
dispersive Raman microscopes configured with automated stages. Once loaded, the Microsampling
samples in the wells can be sampled individually by hand or in an automated fashion with
the Array™ Automation software module for OMNIC software. The Array Automation software
provides a convenient interface to indicate which wells should be analyzed and how
they are analyzed. Wells may be measured with a single collection point in the center of
the well; sampled by summing multiple collection sites within each well to make sampling
of heterogeneous samples more reproducible; mapped to provide a chemical profile of
each well; or flagged for manual selection of the sampling site within each well.

Applications Product Configuration Part Number

•Automated analysis of liquids and Well-plate adapter for Nicolet Almega 470-221100
powders in well-plates Array Automation Software 834-032700
•Drug polymorph screening Nicolet Almega Array Automation Communication Kit 699-080600

Features
•Supports 96 well, 384 well, and 1536
well plates
•Plates held in reproducible positions
with spring-loaded clamping mechanism
•Supports sophisticated data analysis
specific to each well with Array
Automation software
• Well-plate handling robotics available
for automated loading and unloading of
45 or more well-plates

Array Automation software simplifies collection and analysis of well-plate based samples.
96 well-plate

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Notes

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