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unit two

Plant Structure,
Chemistry, Growth,
Development, Genetics,
Biodiversity, and Processes
6 Structure of Higher Plants
7 Plant Growth & Development
8 Plant Chemistry & Metabolism
9 Genetics & Propagation
10 Cultivated Plants:
Naming, Classifying, Origin,
Improvement & Germplasm
Diversity and Preservation
11 Photosynthesis & Respiration
12 Water Relations
13 Mineral Nutrition

Chapter 9 - Genetics and Propogation

KEY LEARNING CONCEPTS


After reading this chapter, you should be able to:

Explain how the basic concepts of genetics relate to


the production and utilization of plants.
Describe the common methods of plant breeding
and sexual and asexual propagation.
Discuss how genetic engineering is used to
introduce genetic traits into plants from unrelated
or distantly related organisms.

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation of Plants

Propagation: The process of increasing plant numbers


Sexual or asexual methods
Preservation of traits

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation of Plants
Line: If the plant group reproduces true by seed
with no characteristics changed
A line is homozygous and, if self-pollinated, seed
propagation yields progeny like the original plant.
Inbred and hybrid lines

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation of Plants
Heterozygous plants require vegetative propagation
Clones: cultivars originating from a single plant or
plant part

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation of Plants

Control of seed source is necessary to maintain a Line


State agencies set standard for seed quality and
characteristics
Breeder seedproduced in small amounts, under the
control of the plant breeder, to produce foundation seed.
Foundation seedmultiplied from breeder seed, available
in limited amounts, planted to produce registered seed.
Controlled by public/private foundation seed stock
organizations.

Registered seedseed source for growers of certified


seed, under the control of the registered seed producers.
The progeny of either breeder or foundation seed.

Certified seedis of known genetic identity & purity,


available in large quantities, sold to farmers for general
crop production.
tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Seed Formation
A seed has three
essential parts:
The embryo, which
develops into the new
plant.
Food storage material, to
nourish the embryonic
plant.
Seed coverings.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Seed Storage and Viability Testing


Germination test- Tests
germination over a
period of time

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Seed Dormancy
Dormancy is an important survival mechanism for some
speciesa result of evolutionary development.
They have survived these factors prevent seed germination of
woody perennials in the autumn, allowing the embryonic plant
in the seed to overwinter in a very cold-resistant form.

Causes for dormancy can persist indefinitely in the


seed and require specific treatments to overcome
them before germination can take place.
Dormancy can result from structural or physiological
conditions in seed coverings, the embryo, or both.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Seed Dormancy
Dormancy - survival mechanism for some species
Prevents germination out of season, or in unfavorable
conditions
Can be structural (seed coat) or physiological (cold
requirement), or both.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Seed Germination
The sequence of events during seed germination:
Imbibition of water by seeds
Activation of hormones/enzymes
Embryo growth/development

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Vegetative Propagation
Vegetative/asexual propagation is done by mitosis.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Vegetative Propagation

Mitotic cell division,


produces adventitious
shoots & roots and callus
(parenchyma cells for healing
of a wound or graft union)

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Vegetative Propagation

Adventitious shoots shoots that


appear any place other than
shoot terminals or axils of leaves.

Figure 9-11 Regeneration in asexual propagation.


Adventitious shoots growing from a root cutting.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Vegetative Propagation

Adventitious roots appear any


place other than from the radicle
(root tip) of the seed or its branches.

Figure 9-11 Regeneration in asexual propagation.


Adventitious roots developing from the base of a stem cutting.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Vegetative Propagation

Callus is a mass of
undifferentiated, proliferating
parenchyma cells.

Figure 9-11 Regeneration in asexual propagation.


Callus tissue produced to give healing of a graft union.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Vegetative Propagation
Environmental factors can modify appearance and
behavior of the plant (phenotype), so that individual
plants can differ strikingly.
Mutation can also cause undesirable changes in clones.

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Disease Problems in Clones

Certification programs are established to provide


nurseries with sources of true-to-name, pathogenfree propagation material.
Culture-indexed or certified cuttings

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Cuttings

Cuttings can be classified according to the part of


the plant from which they are obtained:
Stem cuttings have terminal or axillary buds, but new
roots must develop before a new plant will be formed.
Leaf cuttings have no buds or roots, so both must form.
Leaf-bud cuttings have a bud at the base of the petiole,
for the new shoot systemso only new roots must form.
Root cuttings must produce a new adventitious shoot
and continue growth of the existing root piece, or develop
roots from the base of the new shoot.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Cuttings

Figure 9-14 Types of cuttings:


(A) Hardwood stem cutting. (B) Leafy stem cutting. (C) Leaf cutting. (D) Leaf-bud cutting. (E) Root cutting.
tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding


Graftingjoining parts of plants together to grow as
one plant
Scion part of the graft combination that is to become the
upper or top portion of the plant.
Rootstock lower part of the graft combination that is to
become the root system.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Whip Grafting


Whip Grafting is useful in grafting together
material of about 0.25 to 0.5 diameter.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Whip Grafting


Whip Grafting is useful in grafting together
material of about 0.25 to 0.5 diameter.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Cleft Grafting


The cleft graft is mostly used in top-grafting, where scions of
0.25 to 0.5 diameter are inserted into stubs of older limbs
3 to 4 in diametercut off about a foot from the trunk of the tree.

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Cleft Grafting


It is important in cleft grafting to match the cambium layer
of the scion as closely as possible with the cambium layer
of the stock so that the two pieces heal together.

Cleft grafting is
usually done in
late winter or
early spring.

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Cleft Grafting


Proper selection of scionwood and budwood is very
important in all types of grafting and budding.
Scionwood & budwood
should be taken from
source trees true-to-type
for the cultivar to be
propagated.
They should be free of
known viruses and
any other diseases.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Cleft Grafting


Polarity must be observed: the scions must be
inserted so that the buds point upward.

With woody plants, grafting


and budding must be done
with dormant buds on the scion.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Cleft Grafting


In all types of grafting and budding, the two parts must be held
together very tightly and securely by wedging, tying, nailing, or
wrapping with string, rubber bands, or with plastic or cloth tape.

The graft union must also be


completely covered by grafting
wax to prevent the cut surfaces
from drying out.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Healing

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Healing

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Healing

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Incompatibility

Even when grafts are compatible,


grafted plants can at time be
identified by a swelling where
the graft was made.
This can become a weak point,
susceptible to breaking/cracking.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Layering

Simple Layering:

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Layering


Layering:

Tip Layering

Mound Layering

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation by Grafting & Budding - Layering


Air Layering :

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Other Plant Structures - Runners


Stolons:

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Other Plant Structures - Suckers


Suckers: adventitious shoots from the horizontal
root system, which spread to form a dense thicket.

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Propagation Using Specialized Stems and Roots


Bulbs, corms, tubers, tuberous roots, or
rhizomes can be used for propagation, too.

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Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

Chapter 9 - Genetics and Propogation

Micropropagation (Tissue Culture)


Very small pieces of plant tissue grown on sterile
nutrient media under aseptic conditions.
Explants are used to regenerate new shoot systems,
which can be separated for rooting & growing to full-size.

tab

Practical Horticulture 5th edition


By Margaret J. McMahon, Anton M. Kofranek and Vincent E. Rubatsky

2011, 2007, 2002, 1988 Pearson Education, Inc.


Pearson Prentice Hall - Upper Saddle River, NJ 07458

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