EmilyBurich

APBiology
10/8/15

OsmosisandDiffusionLab/PotatoLab

Objectives:
1. Usedialysistubingtoinvestigatetherelationshipbetweensolute
concentrationandthemovementofwaterthroughaselectively
permeablemembranebytheprocessofosmosis.
2. Usepotatocoresplacedindifferentmolarconcentrationsofsucrose
todeterminethewaterpotentialofpotatocells.

OsmosisandDiffusionLab

Materials:
Dialysistubing
Yarn
Distilledwater
Pipets
6Beakers
Scale
0.2Msucrose
0.4Msucrose
0.6Msucrose
0.8Msucrose
1.0Msucrose

Procedure:
1. Cut6stripsofpresoakeddialysistubing.
2. Tieaknotononeendofeachpieceofdialysistubingtomake6
bags.Pour1525mLofeachsolutionintoseparatebags.Twistends
andtieoffwithyarn.
3. FindtheinitialmassofeachbagandrecordinTable1.2.Expressin
grams.
4. Puteachbagintoseparateemptybeakersandlabelthemolarityof
thesolutionineachdialysisbag.


EmilyBurich
APBiology
10/8/15

5. Filleachbeakerabouttwothirdsfullwithdistilledwatersothateach
bagiscompletelysubmerged.
6. Letthemstandfor30min.
7. After30minremovethebagsfromthewater.Carefullydryeachbag
anddeterminethemassofeachbag.
8. RecordyourgroupsdatainTable1.2.Obtaindatafromtheotherlab
groupsinyourclasstocompleteTable1.3.

Analysis:
Table1.2GroupData

Contentsin
DialysisBag

InitialMass

FinalMass

MassDifference PercentChange
inMass

a).0.0M
DistilledWater

6.80g

7.55g

0.75g

11.03%

b)0.2M
Sucrose

6.80g

8.40g

1.60g

23.53%

c)0.4M
Sucrose

5.91g

7.76g

1.85g

31.30%

d)0.6M
Sucrose

5.13g

6.78g

1.65g

32.16%

e)0.8M
Sucrose

6.14g

7.69g

1.82g

29.64%

f)1.0MSucrose 6.58g

9.50g

2.92g

44.38%


EmilyBurich
APBiology
10/8/15

Table1.3ClassData

PercentChangeinMassofDialysisBags

Group1 Group2 Group3 Group4 Group5 Group6 Total

Class
Average

a).0.0
10.93%
M
Distilled
Water

11.03%

4.91%

2.26%

9.9%

22.61%

61.64%

10.27%

b)0.2M 22.22%
Sucrose

23.53%

9.70%

14.47%

5.6%

13.65%

89.71%

14.86%

c)0.4M 16.34%
Sucrose

31.30%

13.05%

22.92%

19.2%

31.84%

134.65% 22.44%

d)0.6M 23.21%
Sucrose

32.16%

16.83%

4.28%

16.7%

24.45%

117.63% 19.61%

e)0.8M 24.96%
Sucrose

29.64%

21.08%

25.13%

21.5%

37.66%

159.97% 26.66%

f)1.0M 24.15%
Sucrose

44.38%

17.71%

32%

15.5%

27.33%

161.07% 26.85%


EmilyBurich
APBiology
10/8/15

Question#10
a. Theindependentvariable:molarity
b. Thedependentvariable:percentchangeinmass

Questions14
1. Thegreaterthemolarityofsucroseinthesolution,thegreaterthe
changeinmass.
2. Bagsthathadamolaritygreaterthan.4wouldswell,waterwould
moveintothebag,andthebagwouldgainmass.Bagsthathada
molaritylessthan.4wouldlosemasstothesolutioninthecup.
3. Changesinmassdonotshowtheproportionalaspectofthesolution,
makingitlessaccurate.
4. P ercentChangeinM ass = FinalMassInitialMass
100
InitialMass
P ercentChangeinM ass = 18g20g
20g 100
P ercentChangeinM ass = 10%

PotatoCoreLab

Materials:
Potatocorer
Potato
Beakers
Distilledwater
0.2MSucrose
0.4MSucrose
0.6MSucrose
0.8MSucrose
1.0MSucrose
Scale


EmilyBurich
APBiology
10/8/15

Procedure:
1. Pour100mLof0.2Msucroseintoalabeledbeaker.Sliceapotato
intodiscsthatareapproximately3cmthick.
2. Useacorkborertocut8potatocylinders.Donotincludeanyskinon
thecylinders.Youneedfourpotatocylindersforeachbeaker.
3. Findthemassofeachgroupof4cylindersandrecordinTable1.4.
4. Droppotatocylindersintobeakersandsealwithplasticwrap.Make
surepotatoesaresubmerged,andleavesitovertheweekend.
5. Removepotatocoresfrombeakers,drythemgentlyonapaper
towel,anddeterminetheirtotalmass.RecordinTable1.4.

Analysis:
Table1.4:PotatoCoreIndividualData
Contentsin
Beaker

InitialMass

FinalMass

MassDifference PercentChange
inMass

a)0.2M
Sucrose

7.12g

7.21g

0.09g

1.26%

b)0.2M
Sucrose

2.28g

7.57g

0.29g

3.98%

Table1.5:PotatoCoreResultsClassData

Contentsin
beaker

Trail1

Trail2

Total

Average

0.0MDistilled
Water

19.76%

19.67%

39.43%

19.72%

0.2MSucrose

1.26%

3.98%

5.24%

2.62%

0.4MSucrose

9.97%

9.90%

19.87%

9.94%

0.6MSucrose

16%

16.4%

32.4

16.2%

0.8MSucrose

21.1%

23.21%

44.31%

22.15%

1.0MSucrose

15.38%

16.19%

31.57%

15.79%


EmilyBurich
APBiology
10/8/15

Question#10:
a. Molarconcentrationofsucrose=0.2M