Metodo integral par

T (h)

[X] (mg/L)
[S](mg/L)
0
0.8101153505
2
0.8633540373
4
1.4667258208
6
2.2120674357
8
2.1322094055
10
2.1614906832

1/t (h)
16.368
12.768
12.108
9.348
7.968
7.848

Utilizando herramienta solver se tienque:

0.7500

0.0000
0.5000
0.2500
0.1667
0.1250
0.1000

0.1800
0.1600
0.1400
0.1200

f(x) = 1.4066
R = 0.99727

0.1000
y [In(S/So)/t]

0.0800
0.0600
0.0400
0.0200

R^2 =

0.0791

0.0000
0.0000

0.0200

M ETODO INTEGR

Conociendo el valo de (a) se tiene que a = Y[x/s]/Xo Ec 1

Se tiene que Y[x/s];
Yx /s

0.6075865129 mg [X]/mg[s]

Para la constante (b) se tiene que:

Igualando la Ec 2 a '0' y utilizando la herramienta solver se determinan los para

De esta Forma:
Funcion objetivo

0.0000

Celdas Cambiantes:

b=
Ks =

2.2772
13.8597

Para la constante (c) se tiene que:

Igualando la Ec 3 a '0' y utilizando la herramienta solver se determinan los para
Funcion objetivo

Celdas cambiantes

0.00

c
M

1.0000
4.0762E-03

Metodo integral para determinar parametros de monod

d (S-So)

x= [ln(1+ad)]/t

y= [In(S/So)/t]

0.0000
0.0532
0.6566
1.4020
1.3221
1.3514

0.0000
0.0196
0.1001
0.1198
0.0861
0.0700
0.3955

Suma

suma(x*y)
x^2
0.0000
0.0000
0.0318
0.0006
0.1484
0.0149
0.1674
0.0200
0.1210
0.0104
0.0981
0.0069
0.5667
0.0528

0.0000
0.0004
0.0100
0.0143
0.0074
0.0049
0.0371

f(x) = 1.4066490675x + 0.0017259053

R = 0.997278926

0
0000

0.0200

0.0400

0.0600

0.0800

0.1000

0.1200

X [ln(1+ad)]/t
M ETODO INTEGRAL

Linear (METODO INTEGRAL)

Linear (M ETODO INTEGRAL)

Ec 2

0.1400

er se determinan los parametros (b) y Ks

g/L

er se determinan los parametros (c) y M

Ec 3

1/h

y^2
0.0000
0.0010
0.0220
0.0280
0.0146
0.0096
0.0753

0.1400

C
#Tubo
1
2
3
4
5
6

Peso tubo seco (g)

11.7814
12.1941
12.3866
12.5023
12.3769
12.3468

Tiempo (h)

FD
0
2
4
6
8
10

Peso del tubo +clulas secas (g)

11.816
12.232
12.427
12.55
12.429
12.401

50
50
50
50
50
50

CURVA PATRN
Biomasa (g/10 ml)
0
0.0379
0.0404
0.0477
0.0521
0.0542

DNS (Biorractor)

Absorbancia
0.24
0.18
0.169
0.123
0.1
0.098

Tiempo (h)
0.036
0.042
0.11
0.194
0.185
0.1883

1
2
3
4
5
Total
# Cel/ml

N
ml H20
10
8
6
4
2
0

ml de levadura
0
2
4
6
8
10

Biomasa (g/L)
0
3.79
4.04
4.77
5.21
5.42

Absorbancia (nm)
0
0.215
0.373
0.467
0.545
0.6857

2
Cuadro
8
5
6
7
13
39
39000

3
5
6
1
0
15
15000

12
18
17
19
22
88
88000

25
26
21
30
28
130
130000

19
12
11
14
17
73
73000

Calculo de la biomasa apartir de la cuerva patron y la absorbancia rggistrada en el fe

tiempo (h)
Absorbancia
[x] (g/L)
Log [x]
0
0.036
0.8101153505 -0.091453
2
0.042
0.8633540373 -0.063811
4
0.11
1.4667258208 0.1663489
6
0.194
2.2120674357 0.3447984
8
0.16
2.1322094055 0.3288299
10
0.175
2.1614906832 0.3347534

Tubo

curva patro der DNS

ml sln estandar
ml de agua
1
0
2
0.2
3
0.4
4
0.6
5
0.8
6
1

ppm []
1
0.8
0.6
0.4
0.2
0

0
200
400
600
800
1000

10
11
10
13
9
12
55
55000

a rggistrada en el fermentador batch

Abs 575 nm
0
0.047
0.147
0.266
0.371
0.49

tiempo (h)

DNS (Biorractor)
0
2
4
6
8
10

0.24
0.18
0.169
0.123
0.1
0.098

ppm []

[s] (g/l)
545.6
425.6
403.6
311.6
265.6
261.6

16.368
12.768
12.108
9.348
7.968
7.848

Log [x] vs Tiermpo (h)

0.4
0.3
0.2
0.1
0

10

-0.1
-0.2

Abs 575 nm vs concentracion de glucosa

0.6
0.5

f(x) = 0.0005058571x - 0.0327619048

R = 0.9870460328

0.4
0.3
0.2
0.1
0

200

400

600

800

1000

1200

[s] g/L vs Tiempo (h)

18
16
14
12

[S] g/L
l

10
8
6
4
2
0

Tiempo (h)

10

h)

10

12

glucosa

1000

1200

h)

10

12

Tiempo (h)
0
2
4
6
8
10

[x] (g/L)
[s] (g/l)
0.81011535
16.368
0.86335404
12.768
1.46672582
12.108
2.21206744
9.348
2.13220941
7.968
2.16149068
7.848

[x] g/L vs Tiempo (h)

f(x) = 0.0017480498x^4 - 0.04087127x^3 + 0.2906792246x^2 - 0.4345302648x + 0.8198792975
R = 0.9888627739

[x]

1/[

4.5
4
3.5

f(x) = 1.3843010298x - 0.0

R = 1

3
2.5

0.5
0

1.5
1

2.5
2

3.5
3

4.5
4

5.5
5

Tiempo

6.5
6

7.5
7

8.5
8

9.5
9

10.5
10

1/u 2
1.5
1
0.5
0

0.5

1.

T (h)

h
[X] (mg/L)
[S] (mg/L)
d[X]/dt
2 0.86335404
12.768 0.30168589 0.34943474
4 1.46672582
12.108 0.37267081 0.25408348
6 2.21206744
9.348

Modelo de Langmuir

Modelo de Lineweaver-Burk
(1/h)
1/
[S] (mg/L)
1/[S]
0.34943474
12.768 0.0783208 2.86176471
0.25408348
12.108 0.08259002 3.93571429

(1/h)
0.34943474
0.25408348

1/[S] 1/u

4.5
4
3.5

[S]/u vs [s]

f(x) = 1.3843010298x - 0.0258295437

R = 1

3
2.5

100

[s]/u

1/u 2

50
0
10

1.5

[s]

1
0.5
0

f(x) = 1.4953351133x - 6.984

R = 1
15
20
25
30

0.5

1/M
Ks/M
M
Ks
R2

t (h)

Y x/s

1.51/[s]

2.5

3.5

1/M
Ks/M

0.0258
1.3843
Despejando
38.760 1/h
53.655 g/L
1.000

[x](mg/L)
[s] (mg/L)
(So - S)
0 0.81011535
16.368
2 0.86335404
12.768
4 1.46672582
12.108
6 2.21206744
9.348
8 2.13220941
7.968
10 2.16149068
7.848
0.1834 g [x] / g [s]

M
Ks
R2

(X -Xo)
0.000
3.600
4.260
7.020
8.400
8.520

0.000
0.053
0.657
1.402
1.322
1.351

Modelo de Langmuir

Modelo de Eadie-Hofstee
/[s] (L/mg.h) [S] (mg/L)
0.02736801
12.768
0.02098476
12.108

[S] (mg/L) [S]/ (mg.h/L)

12.768 36.5390118
12.108 47.6536286

[S]/u vs [s]

Chart Title

00

50
0
10

(1/h)
0.3494347379
0.2540834846

0.4
0.2

f(x) = 1.4953351133x - 6.9844387271

R = 1
15
20
25
30
35
40

[s]

f(x) = 0.7237590931x + 0.0011769155

R = 1
0.05 0.1 0.15 0.2 0.25 0.3 0.35

M
Ks
R2

1.4953
6.9844
Despejando
0.669 1/h
4.671 g/L
1.000

0.0012 1/h
0.7238 g/L
1

(X-Xo) vs (So-S)
1.600
1.400
1.200
1.000

(X-Xo)

f(x) = 0.1834216463x - 0.1745842699

R = 0.8577819395

0.800
0.600
0.400
0.200
0.000
0.000

1.000

2.000

3.000

4.000

5.000

(So-S)

6.000

7.000

8.000

9.000

0.4

(X-Xo)

0.800
0.600
0.400
0.200
0.000
0.000

1.000

2.000

3.000

4.000

5.000

(So-S)

6.000

7.000

8.000

9.000

1769155

0.3

0.35

0.4