92 element was considered, Zn was highly concentrated followed by Mn, Cu, Pb and Cd in organs of both the species. Increased metal concentration in liver and kidney was noted for most of the metals except Zn in gonads of W. aitu and Zn, Mn and Cd in gills of L. gonius. The highest Zn concentrations analyzed was found in gonad of W. aftu which was nearly 14 times higher than muscle while the same in L. gonius was nearly equal to muscle, In L. gonius highest concentrations of Zn, Mn and Cd was found in gills. Lowest concentrating ability for all the metals in W. attu was muscle while the same for L. gonius were gonad and muscle. Concentrations of various metals (g/g dry tissue) in muscle of both the fish ranged as follows: 52.5-60.0 for Zn, 5.8-23.0 for Cu, 26.0- 32.5 for Mn, 21.8 -24.4 for Pb and 1.4.3.8 for Cd. Although the variation between species was marginal for Zn, ‘Mn and Pb , it was significantly higher in L. gonius than W. attu for Cu and Ca. Safety aspects of fish muscle was analyzed by comparing the concentrations of heavy metals with those of the WHO permissible limit which indicate safe level for Zn and Cu in muscle of both the fishes and Cd in W. attu and higher levels of Cd in L. gonius and Pb in both the species. This indicates health hazards to local consumer with respect to Pb and Cd. Higher oad of Pb can be explained by the fact that the beel was located along the roadside and connected to river receiving pollution burden. (© - AEH 07 a EFFECT OF PRE-FREEZING TEMPERATURE ON THE SURVIVAL OF VEGETATIVE THALLI OF SOME ECONOMICALLY IMPORTANT SEAWEEDS OF INDIA P-L. Lolrinsanga', Geetanjali Deshmukhe?, S.K. 8% Indian Fisheries Forum, November 22-26, 2008, Kolkata, India Chakraborty, Alkesh Dwivedi? and Soma Das, 'Cenital institute of Freshwater Aquaculture, Kausclyaganga, Bhuboneswar 751002, Orissa, *Cental Institute of Fisheries Educotion, 7+ Bunglows, Versova, Andheri (W], Murabai1 * soma706@gmail.com ffect of prefreezing temperature on the regenerative efficacy of vegetative thalli (apical tips) of Gracilaria corticata (J. Agardh.) J. Agardh., Ulva lobata Kiietzing and Hypnea musiformis (Wulfar) Lamouroux, were carried out in the present study. Survival rate of algal thalli at predetermined concentration level of cryoprotectant, and different prefreezing temperature, equilibration time, thawing temperature and the regenerative efficacy of cryopreserved thalli were evaluated. In order to study the effect of different prefreezing temperature on the vegetative thalli, each sample was directly thawed after prefreezing incubation at different temperature of -20°C, -30°C, -40°C, -50°C and -60°C without immersion in the liquid nitrogen (LN) and cultured. The survival percentage was compared with the survival percentage of the thalli, which were thawed after LN immersion. This study revealed that, for every species viz. Gracilaria corticata, ‘Hypnea miusiformis and Utoa lobata, the survival of the vegetative thalli decrease gradually with the decreasing prefreezing temperature. The optimum prefreezing temperature was -40°C with LN treatment and -20°C without LN treatment. © - AEH 08 ASSESSMENT OF ARSENIC PROFILES IN SOME AQUATIC ELEMENTS OF ARSENIC AFFECTED/AREAS OF WEST BENGAL Parimal Sardar*, S. Chanda, B.K. Mahapatra, S. Dotto Table: Survival of vegetative thelli (apical tips), which were thawed directly after slow cooling without liquid nitrogen treatment Prefreezing Survival (%) . Temperature (°C) Hypnea musiformis Ulva lobata Gracilaria corticata -20 78.78 + Lt 83.46 + 3.3 93.69 £29 - 30 68.29 +23 72.87 2 25 91.38 + 20 - 40 46.87 + 26 57.65 £28 87.08 + 16 = 50 37.39 £31 5242 42.5 80.78 = 13 -60 28.82 +23 30.25 + 3.4 72.52 £22= Indian Fisheries Forum, November 22-26, 2008, Kolkata, India nd G.H. Pailan, Ceniral Instituie of Fisheries Education Universi), Indion Council of Agricultural Research, ola Cente, 32'GN Block, Sector, Salt Lake, Kalkato- 091, tparimalsardar2004@yahoo.co.in ifferent aquatic elements such as ground water (shallow tube well water & shallow pump water), pond sediment, pond water (surface, column and bottom), plankton, benthos, aquatic weeds and fish tissues were collected from Kolsur and Hadipur Jhikra I Gram Panchyat (GP) of Deganga Block, North 24 Parganas, Chandpur and Adampur GP of Haroa Block, North 24 Parganas, Baruipur I and Ramnagar GP of Baruipur Block, South 24 Parganas and Belda GP of Narayangarh Block, Midnapore, West Bengal for estimation of total arsenic. Total arsenic (mg/L or mg/kg) was estimated by standard HGAAS method. Among different GPs, highest total arsenic concentration (mg/L or mg/kg) obtained from each clement of Kolsur GP followed by Baruipur I GP with lowest concentration in the elements of Belda GP. ‘Among different elements, highest concentration of total arsenic was found in pond sediment followed by benthos, plankton and aquatic weeds with lowest concentration in surface pond water of every GP. Among water, hand tube well water and shallow pump water contained significantly (P<0.05) higher concentration and surface pond water contained significantly (P<0.05) lower concentration of total arsenic. It was observed that average total arsenic concentration of hand tube well water, shallow pump water, column and bottom water of ponds of every GP except Belda was more than 0.05 mg/L, whereas each category of water of Belda GP showed total arsenic level below 0.05 mg/L. Surface water of pond of every GP contained average total arsenic concentration below the level of 0.05 mg/L. Except Belda, all other GPs showed mean total arsenic concentration (mg/L) in.hand tube well water and shallow pump water was beyond the permissible limit of 0.05 mg/L (WHO, 1984). In all fish species, it was noticed that fish of Kolsur GP contained significantly (P<0.05) highest total arsenic concentration followed by fish of Baruipur I, Hadipur Jhikra I, Ramnagar, Chandpur and Adampur respectively and lowest concentration observed in fish of Belda GP. Among different fish, bottom welling common carp (Cyprinus carpio) and mrigal 93 (Cirrhinus mrigala) contained higher level of total arsenic in tissues followed by column dwelling rohu (Labeo rokite) and surface dwelling catla (Catla catla) respectively indicating correlation with arsenic concentration of different layer of pond water. Among different tissues studied, liver of each fish contained higher concentration of total arsenic followed by kidney and muscle respectively. It may be concluded that fish tissues of affected GP particularly Kolsur of Deganga Block containing alarming concentrations of total arsenic might not be suggested for human consumption and also might be restricted for selling these fish at the market of nearby cities including Kolkata otherwise it might cause arsenic exposure and ill health in unaffected healthy city dwellers (© ~ AEH 09 INFLUENCE OF DISSOLVED ORGANIC CARBON, TYPE AND CONTENT OF CLAY MINERALS ON THE TOXICITY OF Pb AND Cd TO CYPRINUS CARPIO Subhendu Datta*, Yudhvir Singh and Parimal Sordor, Cental Insitute of Fisheries Educotion, Kolkata Cente, 32- GN Block, SectorV, Salt Lake City, Kolkata ~ 700091, *subhdatia@ychoo.com , cute and short-term chronic toxicity tests were -onducted with inorganic lead and cadmium solutions at different dissolved organic carbon (DOC), Kaolinite and bentonite concentrations. The average length and weight of the test fish, Cyprinus carpio vax communis were 3.25 + 0.83 em (min.), 4.03 + 0.83 cm (max) and 0.61 + 0.24 g (min) 1.07 + 0.38g {max.) respectively. LC, values at 96h and corresponding 95% confidence limits were calculated with the help of Trimmed Spearman-Karber method. Short-term chronic toxicity (NOEC, LOEC) were determined by Dennet’s t-test. Lead and cadmium was found to be most toxic to common carp in demineralised water (96h LC,, = 2.33 and 0.37 ppm respectively). Seven days NOEC and LOEC for mortality were also very low (e.g, <0.05 to 1.0 ppm) When dissolved organic carbon was included with the water medium at different concentrations (ie 0.42, 0.84 and 1.68 mg/L) toxicity of lead and cadmium was found to decrease. 96h LC,, for Pb increased from 4.27 to 7.20 ppm and for Cd increased from 0.66 to 1.24 ppm. Bifect of DOC in reducing the