Study of Mass Transfer in Viscous Fermentations

CMB is an Engineering Center of Excellence funded by the Danish Research Agency.
It is a
collaboration between an acknowledged research manager, his/her institute and university,
and the Research Agency. An Engineering Center of Excellence is a research institute of
first-class quality with tradition for cooperation with industry.
Study of Mass Transfer inViscous Fermentations

- using a Rotating Jet Head Mixing System
Study of Mass Transfer in Viscous

Fermentations
David Kold
Ph.D. Thesis
April 2010
Center for Microbial Biotechnology
Department of Systems Biology
Technical University of Denmark
Building 223
DK-2800 Kgs. Lyngby
Denmark
Phone:
+45 4525 2690
Fax:
+45 4588 4148
www.cmb.dtu.dk
David Kold
ISBN-nr: 9788791494918


Frontpage: Broth from two Aspergillus oryzae fermentations.

Left: Fermentation conducted in a Rotating Jet Head fermenter system.
Right: Fermentation conducted in a impeller mixed tank
Copyright David Kold 2010

ISBN-9788791494918
Printed in Denmark by
WWW.FRYDENBERG.DK
Baldersgade 12 - 16
DK-2200 Copenhagen N

Abstract

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Intracellular Shear Stress
Response in Aspergilli
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Intracellular Shear Stress Response in Aspergilli

4.1 Abstract
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Effect of Viscosity on kLa during a

Filamentous Fungus Fed-Batch
Fermentation
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I8
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Chapter 5
Effect of Viscosity on kLa during a Filamentous

Fungus Fed-Batch Fermentation

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Characterization of Mass Transfer
in a Rotary Jet Head Fermenter
during Xanthomonas campestris
fermentations
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Chapter 6
Characterization of Mass Transfer in a Rotary Jet

Head Fermenter during Xanthomonas campestris
Fermentations
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' '
!
'

!

)=4
7!0 % &"5

6.4.4 Suitability of the RJH system for aeration of Xanthomonas

campestris fermentation broths
%&" '
! " K
'
!
$ '
' ! 7!*
' (,.
# ' !,
)44? !
:>
, =4? ! ,
74
*8!
%&"
$!1 '
=4?! $
==!* )(4!)99.,
7: ) 8> )=> )*8
7!* $ ! 1
' 8* 7!=! '

' !
5 ))*) 8*
I)) #=
!

)=)
7!0 % &"5

120
O2 saturation [%]
-1
Airflow [l min ]
100
80
60
40
20
0
0
10
20
30
10
20
30
40
50
60
70
80
40
50
60
70
80
120
-1
Airflow [l min ]
O2 saturation [%]
100
80
60
40
20
0
Time [h]
4%2( G H ,,

1+ !&)>
'1"%G (6$
%: ,(8-%

! , ( 7!=.
8* !
,
0,= (#0,=. ,= (#,=.
7!7! #0,= #,=
' 84 ! "
,

)==
7!0 % &"5

!
,
84 $ , !,
'
!
*7 #0,= #,=
( 7!7.!
0.040
0.030
-1
-1
q(CO2) q(O2) [mol l h ]
0.035
0.025
0.020
0.015
0.010
0.005
0.000
0.040
0.030
-1
-1
q(CO2) q(O2) [mol l h ]
0.035
0.025
0.020
0.015
0.010
0.005
0.000
0
10
20
30
40
50
60
70
80
Time [h]
4%4( $!3G'"$!G'",

1+
!&)>'
1"%G (6$%: ,(8-
%
*$3
,% ' # *!)! 7!N$3

)=:
7!0 % &"5

! =4 ,%
' )44?$3 4
*4)! '
!
' ( 7!*.
$3 ~4!)* ) !
' (:484. ( 7!8.
( 7!>. ( 7!*. $ $3
4!)=) 4!4*)!
8)
$3 ! =4
$3 4!48)!

( *7.,% $3 4!4))
!
0.50
0.30
-1
kLa [s ]
0.40
0.20
0.10
0.00
0
10
20
30
40
50
60
70
80
Time [h]
4%5( -0,

,++!&(6
>'(8-$"%
) %&"
!
$
7!>!
84 )!) )!:$:
$ !

)!8)!7$:!

)=8
7!0 % &"5

4!=4!8 $:!
#

!
$
/ !
$3! (
.
$ !

)=*
7!0 % &"5

2.0
1.8
1.6
-3
P/V [kw m ]
1.4
1.2
1.0
0.8
0.6
0.4
0.2
0.0
2.0
1.8
1.6
-3
P/V [kw m ]
1.4
1.2
1.0
0.8
0.6
0.4
0.2
0.0
0
10
20
30
40
50
60
70
80
Time [h]
4%7( ),),

1+ !&)>

'1"%?(6$%??)(
8-%?(0+%
6.4.5 Effect of viscosity on kLa

$3
* # *!8 (.q
(69. r (a.s!F' 7!*
:484
( 7!>. $3 4!)=)4!4*)
!;
$3 ( *.

)=7
7!0 % &"5

# s(# *!:.s
Z4!> 7!I!

=!4
3<($3.
=!*
:!4
:!*
8!4
7!4
*!*
*!4
8!*
8!4
3<(9 .
4%9( +!")+!-0"

+, >,+
,+

)1% ++1
'
+>)@T@;%7> @;%57%
# *!:s'

$3 ' !s'(!!s
X). ! '

s(,
.Z4!7 *!
' %&"
! '
' !!
2 n, 2m O):P 0$ O)8P s Z
4!7N s Z ) ! %&"
' $
'!

)=N
7!0 % &"5

6.4.6 Effect of power input on kLa
8) %&"
! '
)* ! '

!5 ()74:=4%.
( 7!)4.!
(.
@Z4!4))(+.4!I)(%=Z4!II8.!
3.50
3.00
Pressure [bar]
2.50
2.00
1.50
1.00
0.50
0.00
150
200
250
300
Pump speed [RPM]
350
4%&;(? 1,)

-) &'&4
,+
)))%

5 (69.
) # )!: , (
7!)).!5 7!))
,!
(69X=$1:.!

)=>
7!0 % &"5

40
35
30
DOT [%]
25
20
15
10
5
0
0
Ppump/V [kW m-3]
4%&&( G ),)

),+ +%

$3
*! $3
' r # *!:! ; $ $3
69 'r
7!)=!
0.00
ln(kLa)
-1.00
-2.00
-3.00
-4.00
-2.0
-1.0
0.0
ln(Ppump/V)
1.0
2.0
4%&'(# ),) +

))-0%

)=I
7!0 % &"5

' rZ4!)== (5 7!)= %=Z4!II>.!
'
!!4!72 n,2m O):P!

( 7!)4.
,$3 (W=$1:. !
6.4.7 Effect of superficial gas velocity
%&"Q

/

$3 /
! %&"
$3!

!
%&" !

/ !
# / '
! # $3!1
# '
# !

+ $ $ %&"!

7!>!
!
!
$ $
/
!

):4
7!0 % &"5

! H '
(sZ4!>rZ4!)==.
( !! 84 ( 7!*..! ;
# *!:@
P
k La
ln t ln app
k
V
ln (u s ) = ln
#$% 4%&
H # 7!) ' TK
7!):!
=

)( 7!):.!
ln(kLa/k)-ln(P/V)-ln(app)
0.0
-1.0
-2.0
-3.0
-8.0
-7.0
-6.0
-5.0
ln(us)
4%&.(# ) % +

+1 +>)@@;%/%
&(6>
'(8-$%

'qZ4!8 /
' ' 7!=!

):)
7!0 % &"5

1 4%'( 0 )LUM %
)+6WG+6X=D&.E
#)
>U
)

4!*
%&"

< $ O)*P
4!8
5
# '
%
O)7P
4!:
5

' !
0$ O)8P
4!7N
-

' !
2 n,2m O):P

F' 7!= $3
%&" '
%&"
$!

):=
7!0 % &"5

6.5 Conclusion
' % & "

! 5
( '.$3(4!)*
. ()!)$1:. (4!)99.
!

% &"
$3 '
!

! 5 $3
'
%&"!%&"

!
; '#
*!:( $3
$ . @
P
k L a = ku s0.4 t
V
0.122
( )
0.8
app
#$% 4%'

)::
7!0 % &"5

6.6 References
)!
=!
:!
8!
*!
7!
N!
>!
I!
)4!
))!
)=!
):!
)8!
)*!
)7!

):8
%+!%!F1$0%

F =447!
:I(=.@!)IN!
5 $ !0!+!1! -,
!9!*!)III@&1 j+!=7I*=N))!
2 n,5!F!2!0 %
7

07F
=44)!=>(7.@!*74!
2 n,5!!

$
;
=444!)>(N.@!*8I!
!!

; =44)!:N().@!N:!
; !!+!"!!%" -
%0!=44>; / +H 5 (; /
<H+.+ F < % 5(+!
<H+.@H +!
;$
!!
:
$
; )II>!*4(=.@!)8*!
&
!G!!2"
%

1 & ; )IIN!):(:.@!=7)!

3 5!!G 0%

0

+

; =44)!**(8.@!8)N!

\
!!

*
!)II)!!::4!

!!1
" ,
; )II7!)=(8.@!8778N:!
< &!&!9 2!3 A !=!=44:
<E $@G
6 !*=>!
2 n,5!F!2m 7
%

; F & )II>!)().@!)!
0$!&!0! &!;%

G %(.=!0!; 5 )I>>@!:NS78!
< $ !! *%

7 0 F + =44:!*>()N.@!:>NN!

!2!!$ +
DBG+
-- +

0 F + )II8!8I(7.@!>4:!
Chapter 7
Preliminary Results, Further Work
and Conclusion
N! %5 1 $0

):7
N! %5 1 $0
Chapter 7
Preliminary Results, Further Work and Conclusion

7.1 Preliminary Results
7.1.1 Feed strategy optimization during Aspergillus oryzae fed-batch
fermentations
//

' !"
!

; O)P !
" )* :4?
' >)4 !

'!

' ' %&"!

/ $
%&" '
$ ,=4?!
4!=)99 ,=4?!
!
1 )4) ' $
#(0,=. ! '
)99 ,
=4?!

, ,=4?! N!)
Q ). 4!=99! =. F'
, ' (
4!)).!:., )99!

):N
N! %5 1 $0

-4
250
-8
200
150
-10
100
-12
50
-14
0.00
Airflow [l min-1]
ln(q(CO2))
-6
0
20.00
40.00
60.00
Time [h]
5%&( !$!3G'"" : , )

1+ % + +
1- + )
&
$!3G'"% ) 1+ )+ !&"@ ;%'/+ %
) ,+ !'"@
&
;%&;+ %

H
!"
' !
7.1.2 Cellulase production with Trichoderma reesei

!

! $

$ !
!
' %&"
!

3 3 / & ! %0:4
=:0 %&" ) $ ==*

):>
N! %5 1 $0
=*) (

.
"8!*(!! 3 3
*3
.! $ 4!))99
' )!)$1:!
5 H
(5H). - !O=P!

H ( !)74.
# (
.!
!
" $
!

$ ( 8.
! +
8!85H)
(! * $
!=44.!

%&" ! "
%&"

):I
N! %5 1 $0

7.2 Further Work

7.2.1 Scale up of the Rotary Jet Head system
# %&"
)* N ! #
:)4 ! "
/
!5 %&"
+,-
6+
! % < $ O:P
%&" ' !
%&"! ;
)*
$ (
. ! )* %&"
/
+,-
6+
!
%&" '
# ! 2
/ ++84
$
3 ' !
$ # ' )= ! "
'
! '

!
7.2.2 Computational Fluid Dynamics

%&" ! "
!
0 (05.
%&" !
/ ! < $ O:P /

)84
N! %5 1 $0
' !05

!
05
05 '
#
!

)8)
N! %5 1 $0

7.3 General Conclusions

/ %&" '
$ !
)* %&"

+,-
6+
!
! %&"

!
%&"
' '
! 0 ' %&"
!+

%&"
' !

"$ 53)4 ' 9
:44
! ;

!

%&"
' (F+.
! $3
$ . @
P
k L a = ku s0.4 t
V
0.122
( )
0.8
app

' $%&"

)8=
N! %5 1 $0
!

' !! F+
! %&"

(05H). !!
! 2
!
$ %&" 0
T K $!

)8:
N! %5 1 $0

7.4 References
)!
=!
:!

)88
; +!G!+!1 !%!

-

$
$
; ; =44:!>=().@!)))))N!
- \!!

H-

0

; ;
=448!))*().@!)))*!
< $ !!% *#

00 F
% =44>!>7()=.@!)8*8!

'
Appendix I

)8*

'
LOCUS
AO090003000770
AO090102000482
AO090103000165
AO090010000018
AO090020000500
AO090020000501
AO090103000167
AO090103000166
AO090012000174
AO090003000392
AO090206000078
AO090009000634
AO090005000652
AO090003001320
AO090701000780
AO090026000144
AO090120000176
AO090102000480
AO090001000203
AO090011000202
AO090001000189
AO090020000412
AO090038000281
AO090120000376
AO090010000443
AO090023000513
AO090005001318
AO090120000175
AO090120000106
AO090009000496
AO090010000243
AO090010000444
AO090005001622
AO090166000070
AO090020000191
AO090701000877
AO090020000685
AO090010000015
AO090011000885
AO090701000669
AO090020000652
AO090005001430
AO090023000857
AO090113000063
AO090011000725
AO090023000382
AO090010000469
AO090701000738
AO090011000266
AO090010000445
AO090003000006
AO090005001107
AO090005000588
AO090011000337
AO090102000388
AO090113000062
AO090011000343
AO090009000212
AO090103000170
AO090012001027
AO090011000699
AO090120000214
AO090012000673
AO090001000738
AO090120000179
AO090011000747
AO090102000479
AO090003001008
AO090005001117
AO090023000318
AO090020000043
AO090701000273
AO090010000061
AO090001000291
AO090012000168
AO090003001067
AO090038000539
AO090010000667
AO090102000297
AO090026000357
AO090023000949
AO090103000367
AO090011000926
AO090023000577
AO090011000149
AO090023000687
AO090003000141
AO090701000353
AO090001000689
AO090003001209
AO090023000514
AO090011000358
AO090009000095
AO090102000525
AO090010000650
AO090005001239
AO090005000739
AO090023000430
AO090023000624
AO090012000601

)87
Coef
F.p.value
-5.0178
0.00001
-4.1403
0.00003
-3.6399
0.00004
-4.371
0.00005
-5.1678
0.00008
-3.8496
0.00009
-3.863
0.00011
-2.6965
0.00012
-2.6367
0.00012
-3.3587
0.00014
-2.3036
0.00015
-5.0503
0.00015
-2.8658
0.00015
-3.7142
0.00017
-2.2457
0.00017
-2.9833
0.00018
-2.2334
0.00023
-3.6024
0.00024
-1.9901
0.00027
-3.1367
0.00030
-3.2998
0.00030
-2.6068
0.00030
-3.4536
0.00030
2.8907
0.00034
-2.8983
0.00035
-2.6358
0.00038
2.362
0.00039
-2.5484
0.00040
-1.8891
0.00040
-2.1146
0.00042
-1.9892
0.00042
-6.0805
0.00044
-7.2937
0.00051
-4.0901
0.00051
-1.8155
0.00052
2.1035
0.00053
-3.3617
0.00057
-2.2193
0.00057
-1.6716
0.00059
-2.0733
0.00061
2.1434
0.00063
-1.5731
0.00066
-1.7779
0.00072
-2.776
0.00072
-2.1464
0.00073
1.7553
0.00073
-3.0017
0.00075
-4.1631
0.00079
-1.6086
0.00080
-5.2369
0.00086
-1.4452
0.00106
-5.2323
0.00110
-4.2382
0.00111
-2.3971
0.00114
-4.7097
0.00117
-1.5935
0.00118
-1.2621
0.00120
-1.9796
0.00120
-1.5088
0.00124
-2.1451
0.00132
-1.6632
0.00137
-2.6266
0.00138
-1.2451
0.00154
-1.953
0.00156
-1.7834
0.00161
-1.8508
0.00163
-2.4377
0.00168
-1.2902
0.00169
-1.8999
0.00170
-1.6671
0.00171
-1.5662
0.00172
-1.2943
0.00176
-1.2358
0.00178
-2.6846
0.00179
1.2855
0.00180
1.1255
0.00181
-1.2284
0.00185
-4.8723
0.00193
-3.5725
0.00194
-3.7372
0.00195
-1.2377
0.00204
-1.6766
0.00207
-2.7726
0.00210
-3.2002
0.00217
-1.3112
0.00223
-1.5366
0.00223
-1.538
0.00233
-1.7783
0.00233
-1.4129
0.00236
-1.0499
0.00243
-2.3417
0.00243
-1.0711
0.00243
-1.0601
0.00245
-1.703
0.00253
-1.1322
0.00256
-2.1096
0.00268
-1.1845
0.00268
-4.9618
0.00300
-3.5175
0.00301
-4.5329
0.00302
PFAM_NAME
Glycos_transf_1
GST_N
MFS_1
p450
KR
KR
PP-binding
ABC_membrane
Patatin
UCH
DUF1774
ADH_zinc_N
AA_permease
CoaE
FHA
MFS_1
FAD_binding_8
GST_N
AMP-binding
NAD_binding_1
Fungal_lectin
RRM_1
F-box
AMP-binding
Zn_clus
DnaJ
FMO-like
Ctr
p450
Cyclin
Zn_clus
PFK
Nexin_C
p450
MFS_1
Transferase
p450
p450
MFS_1
RTC
BBE
Mito_carr
OTCace
Chitosanase
Ank
Peptidase_S10
Ribosomal_S12
Mannosyl_trans2
Acetyltransf_2
DUF833
Proteasome
Sterol_desat
AcetylCoA_hydro
NAD_binding_6
MFS_1
MFS_1
Helicase_C
G_glu_transpept
DUF1749
SWIRM
PhyH
Ctr
Pyr_redox
Yippee
ICL
MFS_1
GST_N
E3_binding
FA_hydroxylase
C4dic_mal_tran
Zn_clus
GRAM
Methyltransf_2
Methyltransf_2
DUF590
FGGY_C
Amidase
Sterol_desat
Thioredoxin
Cerato-platanin
COesterase
FAD_binding_8
Pkinase
2-Hacid_dh_C
NAD_binding_1
PLA2_B
Glyoxalase
Cupin_2
RRM_1
Glyco_hydro_31
DnaJ
Pyr_redox
polyprenyl_synt
HATPase_c
Methyltransf_12
Sec63
GSHPx
NUDIX
Ubie_methyltran
Phosphorylase
PFAM_DESCRIPTION
Glycosyl transferases group 1
Glutathione S-transferase, N-terminal domain
Major Facilitator Superfamily
Cytochrome P450
KR domain
KR domain
Phosphopantetheine attachment site
ABC transporter transmembrane region
Patatin-like phospholipase
Ubiquitin carboxyl-terminal hydrolase
Fungal protein of unknown function (DUF1774)
Zinc-binding dehydrogenase
Amino acid permease
Dephospho-CoA kinase
FHA domain
FAD-binding domain
AMP-binding enzyme
Oxidoreductase NAD-binding domain
Fungal fucose-specific lectin
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
F-box domain
AMP-binding enzyme
Fungal Zn(2)-Cys(6) binuclear cluster domain
DnaJ domain
Flavin-binding monooxygenase-like
Ctr copper transporter family
Cytochrome P450
Cyclin
Phosphofructokinase
Sorting nexin C terminal
Cytochrome P450
Transferase family
Cytochrome P450
Cytochrome P450
RNA 3'-terminal phosphate cyclase
Berberine and berberine like
Mitochondrial carrier protein
Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain
Fungal chitosanase
Ankyrin repeat
Serine carboxypeptidase
Ribosomal protein S12
Mannosyltransferase (PIG-V))
N-acetyltransferase
Protein of unknown function (DUF833)
Proteasome A-type and B-type
Sterol desaturase
Acetyl-CoA hydrolase/transferase N-terminal domain
Ferric reductase NAD binding domain
Helicase conserved C-terminal domain
Gamma-glutamyltranspeptidase
SWIRM domain
Phytanoyl-CoA dioxygenase (PhyH)
Ctr copper transporter family
Pyridine nucleotide-disulphide oxidoreductase
Yippee putative zinc-binding protein
Isocitrate lyase family
e3 binding domain
Fatty acid hydroxylase superfamily
C4-dicarboxylate transporter/malic acid transport protein
GRAM domain
O-methyltransferase
O-methyltransferase
Protein of unknown function, DUF590
Amidase
Sterol desaturase
Thioredoxin
Cerato-platanin
Carboxylesterase
FAD-binding domain
Protein kinase domain
D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Oxidoreductase NAD-binding domain
Lysophospholipase catalytic domain
Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Cupin domain
Glycosyl hydrolases family 31
DnaJ domain
Polyprenyl synthetase
Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Methyltransferase domain
Sec63 Brl domain
Glutathione peroxidase
NUDIX domain
ubiE/COQ5 methyltransferase family
Carbohydrate phosphorylase
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100

'
LOCUS
AO090003000309
AO090026000463
AO090003000331
AO090010000501
AO090003000316
AO090003000624
AO090011000453
AO090010000628
AO090038000253
AO090012000821
AO090005000828
AO090023000349
AO090701000554
AO090005000471
AO090009000493
AO090005000583
AO090120000471
AO090003001519
AO090026000396
AO090026000711
AO090038000500
AO090120000103
AO090701000238
AO090011000867
AO090003000206
AO090102000533
AO090701000343
AO090010000221
AO090010000065
AO090026000248
AO090102000168
AO090023000431
AO090103000273
AO090020000493
AO090038000424
AO090003000906
AO090005001449
AO090003000980
AO090001000555
AO090012000997
AO090012000521
AO090012000652
AO090005001249
AO090005000125
AO090001000692
AO090023000304
AO090103000026
AO090003000831
AO090012000646
AO090005000804
AO090009000484
AO090020000684
AO090120000183
AO090005000161
AO090003000893
AO090206000069
AO090009000228
AO090011000922
AO090038000395
AO090206000052
AO090009000516
AO090023000314
AO090011000875
AO090120000174
AO090701000318
AO090023000528
AO090701000639
AO090038000590
AO090011000757
AO090120000385
AO090012000787
AO090020000504
AO090026000696
AO090038000589
AO090012000228
AO090012000708
AO090038000173
AO090003000459
AO090001000012
AO090023000932
AO090026000066
AO090005000683
AO090005001147
AO090011000454
AO090012000287
AO090012000665
AO090023000147
AO090020000239
AO090023000935
AO090003000661
AO090005000114
AO090009000542
AO090038000427
AO090138000023
AO090010000210
AO090011000884
AO090026000392
AO090005001641
AO090001000273
AO090206000070
Coef
F.p.value
-1.1931
0.00304
1.6762
0.00317
1.0425
0.00320
-1.4612
0.00323
-0.96
0.00340
-1.0527
0.00341
1.3303
0.00345
-1.5827
0.00350
-1.4501
0.00351
-2.5789
0.00356
-2.6115
0.00356
-1.318
0.00369
-1.3302
0.00380
-2.1775
0.00385
-2.5345
0.00390
-5.8382
0.00401
1.9446
0.00407
-1.1893
0.00409
-3.6706
0.00415
2.3163
0.00420
-1.3135
0.00420
-0.9755
0.00423
-1.6135
0.00427
-1.4459
0.00433
-1.3421
0.00434
-2.2958
0.00442
-3.4696
0.00443
-2.8067
0.00444
-2.3072
0.00456
-2.7055
0.00480
1.6445
0.00485
-5.9574
0.00485
0.9435
0.00486
-1.0647
0.00493
-2.2357
0.00515
-1.1774
0.00518
-0.9398
0.00527
-0.8993
0.00533
-1.0955
0.00534
-1.1893
0.00539
-1.0173
0.00542
-1.1532
0.00545
1.1824
0.00546
-1.0043
0.00547
-1.2357
0.00548
-0.8748
0.00555
-0.8365
0.00557
-4.4339
0.00559
-1.5142
0.00566
-1.5601
0.00573
-1.0334
0.00575
-2.6961
0.00578
-3.0748
0.00584
1.1194
0.00596
1.3479
0.00603
-1.7196
0.00613
-2.6348
0.00614
-0.9516
0.00616
-1.264
0.00617
-2.1163
0.00618
-0.89
0.00621
-1.0923
0.00628
-1.8286
0.00631
-2.5831
0.00647
-2.5717
0.00649
-1.2113
0.00660
-1.079
0.00662
-1.1257
0.00671
-2.0636
0.00672
-1.2304
0.00675
-0.794
0.00685
1.1444
0.00693
-1.7182
0.00693
-1.1604
0.00699
-0.8622
0.00704
-0.8327
0.00707
-1.9255
0.00710
-1.5916
0.00714
-0.7805
0.00715
-0.9707
0.00719
-1.7365
0.00725
-0.7843
0.00727
-1.8767
0.00733
1.0459
0.00733
-3.531
0.00739
0.7841
0.00746
-0.9858
0.00769
-0.8545
0.00773
1.2352
0.00774
-6.1244
0.00777
1.8991
0.00779
1.4889
0.00781
-1.5467
0.00786
-1.4156
0.00795
-2.1089
0.00822
-1.1819
0.00823
-1.6463
0.00827
-0.7646
0.00837
-0.7781
0.00839
-1.204
0.00840
PFAM_NAME
GMC_oxred_C
NUC173
Transferase
MFS_1
LSM
Acetate_kinase
CTP_transf_2
ABC_membrane
Ank
Sds3
TPR_2
Transketolase_C
HSP20
MFS_1
Cyclin
Sel1
PalH
AA_permease
MFS_1
MHYT
RasGEF
FAD_binding_8
Abhydrolase_1
Ank
p450
EMP24_GP25L
Dioxygenase_C
Bac_rhodopsin
UDPG_MGDP_dh
MFS_1
p450
NUDIX
MFS_1
Acyl-CoA_dh_M
UPF0261
Glyco_hydro_12
Aldo_ket_red
SurE
E1_dh
MAP1_LC3
NAD_binding_4
KTI12
Acyl-CoA_dh_1
ADH_zinc_N
MFS_1
F-actin_cap_A
Peptidase_S10
FAD_binding_2
HEAT
Helicase_C
Aminotran_1_2
p450
Asn_synthase
Methyltransf_2
ADH_zinc_N
MFS_1
Asp
Hydrolase
PGK
Actin
zf-C2H2
3-HAO
DnaJ_CXXCXGXG
Abhydrolase_2
Aldedh
PP-binding
Glyco_hydro_31
LisH
Acetyltransf_1
GTP_EFTU_D2
Band_7
Thioredoxin
ADH_zinc_N
LisH
Methyltransf_12
Peptidase_S10
NPD
UDG
2OG-FeII_Oxy
RasGEF
DUF917
3-HAO
ADH_zinc_N
PMI_typeI
KR
Peptidase_C14
Cupin_2
NmrA
MutL_C
TPP_enzyme_M
AA_permease
Aldo_ket_red
KR
adh_short
RPEL
MFS_1
HMGL-like
WD40
ADH_zinc_N
MFS_1
PFAM_DESCRIPTION
GMC oxidoreductase
NUC173 domain
Transferase family
LSM domain
Acetokinase family
Cytidylyltransferase
ABC transporter transmembrane region
Ankyrin repeat
Sds3-like
Tetratricopeptide repeat
Transketolase, C-terminal domain
Hsp20/alpha crystallin family
Cyclin
Sel1 repeat
PalH/RIM21
Amino acid permease
Bacterial signalling protein N terminal repeat
RasGEF domain
FAD-binding domain
alpha/beta hydrolase fold
Ankyrin repeat
Cytochrome P450
emp24/gp25L/p24 family/GOLD
Dioxygenase
Bacteriorhodopsin
UDP-glucose/GDP-mannose dehydrogenase family, central domain
Cytochrome P450
NUDIX domain
Acyl-CoA dehydrogenase, middle domain
Uncharacterised protein family (UPF0261)
Glycosyl hydrolase family 12
Aldo/keto reductase family
Survival protein SurE
Dehydrogenase E1 component
Microtubule associated protein 1A/1B, light chain 3
Male sterility protein
Chromatin associated protein KTI12
Acyl-CoA dehydrogenase, C-terminal domain
F-actin capping protein alpha subunit
FAD binding domain
HEAT repeat
Aminotransferase class I and II
Cytochrome P450
Asparagine synthase
O-methyltransferase
Eukaryotic aspartyl protease
haloacid dehalogenase-like hydrolase
Phosphoglycerate kinase
Actin
Zinc finger, C2H2 type
3-hydroxyanthranilic acid dioxygenase
DnaJ central domain (4 repeats)
Phospholipase/Carboxylesterase
Aldehyde dehydrogenase family
LisH
Acetyltransferase (GNAT) family
Elongation factor Tu domain 2
SPFH domain / Band 7 family
Thioredoxin
LisH
2-nitropropane dioxygenase
Uracil DNA glycosylase superfamily
2OG-Fe(II) oxygenase superfamily
RasGEF domain
3-hydroxyanthranilic acid dioxygenase
Phosphomannose isomerase type I
KR domain
Caspase domain
Cupin domain
NmrA-like family
MutL C terminal dimerisation domain
Thiamine pyrophosphate enzyme, central domain
Amino acid permease
KR domain
short chain dehydrogenase
RPEL repeat
HMGL-like
WD domain, G-beta repeat
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200

)8N

'
LOCUS
AO090011000715
AO090701000734
AO090005001060
AO090023000256
AO090011000830
AO090011000096
AO090023000032
AO090011000751
AO090038000404
AO090005000264
AO090038000421
AO090103000438
AO090003000705
AO090103000406
AO090011000122
AO090001000508
AO090023000534
AO090026000278
AO090120000378
AO090010000442
AO090701000537
AO090005001312
AO090005001558
AO090020000119
AO090701000254
AO090026000173
AO090103000023
AO090005000057
AO090005000882
AO090701000109
AO090003001538
AO090010000724
AO090701000534
AO090003001385
AO090012000975
AO090026000570
AO090003000207
AO090005000775
AO090038000067
AO090003001184
AO090001000531
AO090005000315
AO090001000507
AO090020000355
AO090012000054
AO090023000692
AO090005001111
AO090003001124
AO090011000195
AO090005000160
AO090003000593
AO090701000539
AO090120000370
AO090012000624
AO090003000869
AO090001000419
AO090113000137
AO090003001049
AO090023000125
AO090011000865
AO090005001236
AO090012000130
AO090102000589
AO090020000458
AO090701000417
AO090023000370
AO090003001376
AO090020000199
AO090026000648
AO090103000096
AO090026000230
AO090138000045
AO090005001199
AO090003000979
AO090701000791
AO090020000420
AO090003000497
AO090120000256
AO090010000463
AO090003001302
AO090038000463
AO090023000114
AO090010000126
AO090701000579
AO090005000539
AO090012000998
AO090701000219
AO090026000168
AO090012000429
AO090003001112
AO090701000487
AO090011000403
AO090026000514
AO090011000588
AO090001000592
AO090012000396
AO090701000147
AO090023000883
AO090102000063
AO090701000471

)8>
Coef
F.p.value
-2.3787
0.00875
-1.1388
0.00878
-0.8054
0.00879
-1.7246
0.00887
1.2476
0.00887
-0.8563
0.00892
-1.5723
0.00894
-0.9614
0.00899
-1.1468
0.00906
-1.3936
0.00911
-1.4226
0.00912
-0.7205
0.00914
-0.7596
0.00915
-1.4359
0.00918
0.8431
0.00922
-3.2939
0.00924
0.7754
0.00933
-0.7549
0.00943
-0.8566
0.00945
-1.1388
0.00960
0.7078
0.00961
-0.9504
0.00963
-0.8583
0.00965
-0.823
0.00966
-2.1157
0.00979
-1.3536
0.00989
-0.8966
0.00998
1.0205
0.01000
-0.9386
0.01001
-1.0798
0.01013
-1.5739
0.01024
0.7116
0.01028
0.745
0.01037
-0.7333
0.01042
-0.8597
0.01045
0.7819
0.01049
-2.2211
0.01052
-0.8318
0.01056
-1.0951
0.01057
-1.0652
0.01057
-2.4383
0.01061
0.7023
0.01069
-2.8154
0.01084
0.8779
0.01084
1.6215
0.01095
-1.1811
0.01105
-0.9375
0.01114
0.7531
0.01118
0.7849
0.01123
0.7947
0.01127
-0.8933
0.01133
0.8794
0.01135
-0.8864
0.01138
-0.829
0.01142
-0.7771
0.01144
-1.0699
0.01155
-1.1105
0.01160
-1.2574
0.01163
-1.1049
0.01169
-1.2739
0.01173
-1.0116
0.01183
-1.293
0.01187
-0.8694
0.01187
-1.906
0.01188
1.8198
0.01192
0.7823
0.01200
0.808
0.01208
-0.6723
0.01216
-1.151
0.01223
0.8288
0.01234
-0.9339
0.01240
-1.2698
0.01241
-0.6721
0.01242
-1.4648
0.01249
1.743
0.01251
2.7123
0.01253
-0.7626
0.01255
-1.1848
0.01261
-1.0198
0.01265
2.7257
0.01279
-3.3672
0.01283
-1.5288
0.01288
1.0566
0.01288
1.8385
0.01297
1.1651
0.01308
-2.4705
0.01309
-1.2148
0.01314
1.568
0.01333
0.7744
0.01339
-0.844
0.01348
1.8179
0.01359
1.0119
0.01361
-0.7824
0.01363
-1.9969
0.01371
-1.4787
0.01372
2.5861
0.01378
-0.941
0.01379
-1.0157
0.01393
-2.556
0.01407
-1.0484
0.01411
PFAM_NAME
CBM_1
Autophagy_C
Cyt-b5
Amidohydro_1
Endonuclease_NS
HET
Aegerolysin
MFS_1
zf-C2H2
HhH-GPD
UPF0261
3Beta_HSD
GatB_Yqey
Thiolase_C
Ribonuclease
Ketoacyl-synt_C
Glyco_hydro_71
NmrA
Arrestin_N
Zn_clus
ABC_tran
CoA_transf_3
F-box
SURF1
DAO
NmrA
Amidohydro_1
Aminotran_1_2
AAA_2
GIDA
MFS_1
Glyco_hydro_76
MFS_1
ABC2_membrane
FMN_dh
ApbA_C
zf-CHY
PQ-loop
Ank
Transferase
Methyltransf_12
Peptidase_M20
Ketoacyl-synt_C
MFS_1
Ank
Acid_phosphat_A
Syja_N
MOZ_SAS
MFS_1
ATP11
Glycos_transf_2
MFS_1
Amidohydro_2
Pyr_redox
tRNA-synt_2
KR
Fungal_trans
Pro_isomerase
Lipase_3
Ank
ALO
Aldedh
Cu-oxidase
PHD
p450
Acyl_transf_3
ADH_zinc_N
Abhydrolase_3
Myb_DNA-binding
MFS_1
UQ_con
FMN_dh
N2227
SurE
Metallophos
Lyase_1
Glyco_hydro_1
IATP
RmlD_sub_bind
EHN
MFS_1
IF-2B
Sugar_tr
Peptidase_S41
Pyridoxal_deC
Coprogen_oxidas
DnaJ_C
Acyltransferase
ECH
AMP-binding
SGL
COesterase
AMP-binding
Uricase
UQ_con
Abhydrolase_1
MFS_1
Abhydrolase_1
Sulfatase
p450
PFAM_DESCRIPTION
Fungal cellulose binding domain
Autophagocytosis associated protein, C-terminal domain
Cytochrome b5-like Heme/Steroid binding domain
Amidohydrolase family
DNA/RNA non-specific endonuclease
Heterokaryon incompatibility protein (HET)
Aegerolysin
HhH-GPD superfamily base excision DNA repair protein
3-beta hydroxysteroid dehydrogenase/isomerase family
GatB domain
Thiolase, C-terminal domain
ribonuclease
Beta-ketoacyl synthase, C-terminal domain
NmrA-like family
Arrestin (or S-antigen), N-terminal domain
ABC transporter
CoA-transferase family III
F-box domain
SURF1 family
FAD dependent oxidoreductase
NmrA-like family
Amidohydrolase family
ATPase family associated with various cellular activities (AAA)
Glucose inhibited division protein A
ABC-2 type transporter
FMN-dependent dehydrogenase
Ketopantoate reductase PanE/ApbA C terminal
CHY zinc finger
PQ loop repeat
Ankyrin repeat
Transferase family
Peptidase family M20/M25/M40
Ankyrin repeat
Histidine acid phosphatase
SacI homology domain
MOZ/SAS family
ATP11 protein
Glycosyl transferase family 2
Amidohydrolase
tRNA synthetases class II (D, K and N)
KR domain
Fungal specific transcription factor domain
Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Lipase (class 3)
Ankyrin repeat
D-arabinono-1,4-lactone oxidase
Multicopper oxidase
PHD-finger
Cytochrome P450
Acyltransferase family
Myb-like DNA-binding domain
Ubiquitin-conjugating enzyme
N2227-like protein
Survival protein SurE
Calcineurin-like phosphoesterase
Lyase
Mitochondrial ATPase inhibitor, IATP
RmlD substrate binding domain
Epoxide hydrolase N terminus
Initiation factor 2 subunit family
Sugar (and other) transporter
Peptidase family S41
Pyridoxal-dependent decarboxylase conserved domain
Coproporphyrinogen III oxidase
DnaJ C terminal region
Acyltransferase
Enoyl-CoA hydratase/isomerase family
AMP-binding enzyme
SMP-30/Gluconolaconase/LRE-like region
Carboxylesterase
AMP-binding enzyme
Uricase
Sulfatase
Cytochrome P450
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300

'
LOCUS
AO090120000052
AO090001000587
AO090009000294
AO090023000893
AO090005000796
AO090113000010
AO090005001229
AO090120000260
AO090103000084
AO090005000630
AO090003000278
AO090005000690
AO090701000558
AO090005001300
AO090026000164
AO090010000435
AO090001000634
AO090003001250
AO090012000132
AO090005001379
AO090003000208
AO090102000483
AO090005001429
AO090120000334
AO090012000417
AO090012000457
AO090011000679
AO090001000481
AO090026000508
AO090102000016
AO090113000134
AO090026000778
AO090701000127
AO090003000310
AO090103000098
AO090701000709
AO090023000035
AO090001000121
AO090003001094
AO090012000731
AO090005000281
AO090023000039
AO090003000324
AO090023000241
AO090005000995
AO090003000465
AO090020000068
AO090009000541
AO090001000237
AO090001000290
AO090023000499
AO090023000206
AO090011000829
AO090102000622
AO090011000368
AO090012000252
AO090011000221
AO090011000134
AO090012000537
AO090120000158
AO090009000391
AO090102000382
AO090038000268
AO090701000321
AO090012000180
AO090012001016
AO090005000906
AO090001000619
AO090005001110
AO090102000554
AO090010000217
AO090009000374
AO090005000628
AO090005000460
AO090011000414
AO090012000103
AO090005000250
AO090023000278
AO090012000139
AO090011000555
AO090120000414
AO090023000027
AO090009000492
AO090102000246
AO090011000773
AO090005000823
AO090012000112
AO090103000169
AO090026000417
AO090012000839
AO090023000072
AO090023000163
AO090012000560
AO090010000345
AO090023000611
AO090166000090
AO090038000181
AO090102000076
AO090023000966
AO090020000009
Coef
F.p.value
-1.7222
0.01413
-0.6534
0.01420
-0.8955
0.01422
-1.1169
0.01423
-1.2536
0.01428
-0.9786
0.01430
-1.0855
0.01450
-0.9087
0.01451
0.9436
0.01454
-0.9472
0.01454
-0.8432
0.01458
1.601
0.01466
-0.6444
0.01467
-0.8892
0.01501
-0.9035
0.01504
-1.3791
0.01517
0.6549
0.01534
-1.5518
0.01540
-1.4043
0.01571
-0.7874
0.01572
-0.9142
0.01581
-0.805
0.01582
-0.8137
0.01584
-1.0366
0.01594
-0.9382
0.01598
-4.0837
0.01604
-1.386
0.01621
-0.997
0.01624
-1.7855
0.01631
-1.3333
0.01642
-1.1209
0.01644
-0.8872
0.01646
-0.9013
0.01648
-3.7892
0.01651
-0.9485
0.01661
-0.7041
0.01668
0.6597
0.01668
0.7247
0.01705
-0.7331
0.01707
-1.3756
0.01707
1.4568
0.01720
-0.8504
0.01727
-0.6368
0.01734
-1.8159
0.01739
-0.643
0.01767
-2.7845
0.01768
-0.8682
0.01777
0.6962
0.01777
0.7614
0.01794
-0.7087
0.01799
0.6808
0.01810
0.9739
0.01812
0.9428
0.01818
-0.6249
0.01820
-0.7181
0.01834
-0.7622
0.01843
-0.8005
0.01857
0.8949
0.01878
-0.67
0.01901
0.6644
0.01902
-0.8833
0.01929
-0.6836
0.01949
0.8055
0.01955
-1.9915
0.01972
0.6455
0.01984
-0.6311
0.01992
0.6263
0.01995
-1.197
0.02000
-0.668
0.02009
-1.0496
0.02015
-0.7448
0.02019
-0.5712
0.02031
-0.6912
0.02045
-0.7289
0.02053
-1.2975
0.02057
-0.8898
0.02058
-0.9118
0.02071
0.7304
0.02080
0.5697
0.02093
-0.6572
0.02097
-0.6878
0.02100
0.5629
0.02106
-0.935
0.02118
-0.988
0.02118
-1.9186
0.02123
-0.7077
0.02128
-0.5711
0.02136
-0.8887
0.02147
-0.5847
0.02154
0.6021
0.02169
2.2177
0.02177
-0.672
0.02183
0.9122
0.02198
-1.0363
0.02207
-0.8958
0.02210
-0.9539
0.02225
0.568
0.02235
-0.8871
0.02238
-0.6357
0.02244
-1.0519
0.02258
PFAM_NAME
Glyco_hydro_65C
MFS_1
FAD_binding_3
FA_desaturase
MA3
GMC_oxred_C
adh_short
37694
AA_permease
ERO1
KR
KR
Glyco_hydro_31
Metalloenzyme
Helicase_C
RTA1
Na_Ca_ex
YEATS
Hist_deacetyl
NUDIX
Glyoxalase
adh_short
G6PD_C
Glyco_transf_15
Tubulin_C
Pyridoxal_deC
Aminotran_1_2
PB1
MFS_1
FAD_binding_7
DSBA
MFS_1
CAS_CSE1
AOX
Ank
Pkinase
HisKA
Ldh_2
Mannosyl_trans2
Pkinase
MFS_1
MFS_1
Pkr1
Polysacc_deac_1
Sterol_desat
Arrestin_N
eIF2A
Aldo_ket_red
GTP_cyclohydroI
Methyltransf_2
zf-C2H2
Ligase_CoA
Endonuclease_NS
HSP90
2-Hacid_dh_C
MFS_1
Fungal_trans
ADH_zinc_N
PTPA
Glyco_hydro_35
IU_nuc_hydro
p450
Ank
Pec_lyase_C
YL1_C
UPF0061
AMP-binding
DAO
zf-C2H2
GIY-YIG
UPF0183
NmrA
APG6
zf-C2H2
Gp_dh_C
LIP
NUDIX
MFS_1
DUF895
zf-C2H2
ECH
Sugar_tr
Cyclin
Pribosyltran
DUF1605
zf-RanBP
GST_C
ECH
Na_Ca_ex
Peptidase_S24
p450
tRNA-synt_1e
A_deaminase
Transp_cyt_pur
DUF1772
PA14
PALP
ADH_zinc_N
Cyt-b5
PCMT
PFAM_DESCRIPTION
Glycosyl hydrolase family 65, C-terminal domain
FAD binding domain
Fatty acid desaturase
MA3 domain
GMC oxidoreductase
14-3-3 protein
Amino acid permease
Endoplasmic Reticulum Oxidoreductin 1 (ERO1)
KR domain
KR domain
Metalloenzyme superfamily
RTA1 like protein
Sodium/calcium exchanger protein
YEATS family
Histone deacetylase domain
NUDIX domain
Glucose-6-phosphate dehydrogenase, C-terminal domain
Glycolipid 2-alpha-mannosyltransferase
Tubulin/FtsZ family, C-terminal domain
PB1 domain
FAD binding domain of DNA photolyase
DSBA-like thioredoxin domain
CAS/CSE protein, C-terminus
Alternative oxidase
Ankyrin repeat
His Kinase A (phosphoacceptor) domain
Malate/L-lactate dehydrogenase
ER protein Pkr1
Polysaccharide deacetylase
Sterol desaturase
Arrestin (or S-antigen), N-terminal domain
Eukaryotic translation initiation factor eIF2A
GTP cyclohydrolase I
O-methyltransferase
CoA-ligase
DNA/RNA non-specific endonuclease
Hsp90 protein
Phosphotyrosyl phosphate activator (PTPA) protein
Inosine-uridine preferring nucleoside hydrolase
Cytochrome P450
Ankyrin repeat
Pectate lyase
YL1 nuclear protein C-terminal domain
Uncharacterized ACR, YdiU/UPF0061 family
AMP-binding enzyme
GIY-YIG catalytic domain
NmrA-like family
Autophagy protein Apg6
Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain
Secretory lipase
NUDIX domain
Eukaryotic protein of unknown function (DUF895)
Cyclin
Phosphoribosyl transferase domain
Domain of unknown function (DUF1605)
Zn-finger in Ran binding protein and others
Glutathione S-transferase, C-terminal domain
Peptidase S24-like
Cytochrome P450
tRNA synthetases class I (C) catalytic domain
Adenosine/AMP deaminase
Permease for cytosine/purines, uracil, thiamine, allantoin
PA14 domain
Pyridoxal-phosphate dependent enzyme
Cytochrome b5-like Heme/Steroid binding domain
Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400

)8I

'
LOCUS
AO090005000867
AO090009000547
AO090012000463
AO090011000883
AO090026000803
AO090012000711
AO090012000956
AO090005001154
AO090003000795
AO090120000236
AO090012000232
AO090023000205
AO090012000418
AO090701000830
AO090003000627
AO090020000585
AO090003000241
AO090012001017
AO090012000812
AO090003000390
AO090026000443
AO090103000384
AO090120000451
AO090005001242
AO090001000406
AO090206000108
AO090011000488
AO090009000558
AO090003000557
AO090166000068
AO090103000153
AO090103000244
AO090103000021
AO090023000071
AO090038000134
AO090011000654
AO090038000485
AO090005001346
AO090026000537
AO090011000838
AO090020000162
AO090138000069
AO090023000013
AO090010000194
AO090138000144
AO090003000209
AO090026000578
AO090010000472
AO090011000203
AO090012000116
AO090102000581
AO090023000185
AO090701000536
AO090038000433
AO090011000456
AO090011000416
AO090010000032
AO090020000109
AO090701000407
AO090003000625
AO090026000255
AO090001000130
AO090005001295
AO090673000006
AO090166000031
AO090003001407
AO090020000195
AO090003001095
AO090026000263
AO090011000871
AO090003000342
AO090038000148
AO090020000141
AO090023000060
AO090023000902
AO090010000576
AO090009000214
AO090026000079
AO090003001208
AO090102000352
AO090003000149
AO090701000671
AO090012000459
AO090005000295
AO090005001065
AO090020000197
AO090003000919
AO090120000493
AO090011000322
AO090011000391
AO090012000554
AO090010000753
AO090003000722
AO090120000476
AO090003000430
AO090020000455
AO090003001500
AO090003000779
AO090010000390
AO090005000439

)*4
Coef
F.p.value
-0.6781
0.02264
0.5918
0.02270
-0.5606
0.02280
-0.901
0.02281
-1.3336
0.02284
-2.3494
0.02294
-0.9294
0.02298
1.2657
0.02308
-0.5876
0.02326
-0.6355
0.02339
0.8851
0.02341
0.9383
0.02349
0.5697
0.02352
-1.0427
0.02362
-2.3099
0.02379
-0.8155
0.02395
-0.7358
0.02406
-0.6065
0.02411
-0.5481
0.02412
-0.7548
0.02416
-0.9596
0.02423
0.9331
0.02431
-1.0135
0.02435
0.8587
0.02438
-1.5961
0.02449
-1.3119
0.02465
-0.6239
0.02495
-0.8184
0.02501
-0.5895
0.02506
0.5521
0.02509
-0.9821
0.02516
1.6084
0.02522
0.781
0.02525
0.8763
0.02534
0.6248
0.02540
-0.953
0.02552
-0.6599
0.02564
-1.2926
0.02575
-0.695
0.02596
1.3312
0.02602
-0.591
0.02618
0.6242
0.02627
-0.5565
0.02628
-1.0139
0.02632
-1.5895
0.02639
0.5702
0.02656
3.0084
0.02675
-1.5622
0.02697
-0.5924
0.02702
-1.2209
0.02707
-0.5516
0.02710
0.6446
0.02712
0.8612
0.02727
-0.9941
0.02731
-0.7142
0.02731
-1.0151
0.02742
1.1844
0.02763
-1.6523
0.02766
-0.8096
0.02774
-0.5534
0.02785
1.7836
0.02788
0.8415
0.02789
-1.0528
0.02792
-0.5577
0.02793
-0.6915
0.02796
-0.5611
0.02797
-0.5589
0.02812
-0.6925
0.02815
-1.1703
0.02826
0.7679
0.02836
1.2684
0.02843
0.8232
0.02852
0.5896
0.02864
0.7258
0.02865
1.9877
0.02866
2.3212
0.02866
-1.5726
0.02874
0.5172
0.02876
-0.5668
0.02881
-0.7185
0.02887
-0.8375
0.02891
-0.5827
0.02893
0.5855
0.02912
1.0125
0.02915
0.527
0.02938
-0.5477
0.02939
-2.5094
0.02939
-0.5926
0.02946
-0.77
0.02952
-0.8571
0.02956
-0.7331
0.02958
-0.8407
0.02963
-0.7018
0.02963
-1.0668
0.02971
-0.6264
0.02972
0.6572
0.02981
0.7077
0.02981
-0.5362
0.02982
0.7413
0.02984
-0.8894
0.02987
PFAM_NAME
Sugar_tr
Amino_oxidase
F-box
MFS_1
AAA
XFP
APH
MFS_1
DegT_DnrJ_EryC1
Pkinase
2-Hacid_dh_C
XAP5
MS_channel
FA_desaturase
RTA1
WW
PGAM
UPF0061
Pkinase
UCH
3HCDH
CFEM
Abhydrolase_1
SPRY
MFS_1
ACPS
FA_desaturase
Gln-synt_C
TTL
Prenyltrans
Peptidase_S10
Tannase
Aldedh
polyprenyl_synt
RTA1
MR_MLE
MFS_1
TBCC
GDI
Biotin_lipoyl
BBE
KR
Saccharop_dh
Glycos_transf_1
F-box
Glyoxalase
Transferase
PPR
MIP
GST_C
CRAL_TRIO
Dynamin_N
ABC_tran
HMG_box
COQ7
p450
Pec_lyase_C
Coq4
Hydrolase
XFP
K_trans
Ribonuclease
AMMECR1
TPR_2
AA_permease
ADH_zinc_N
Methyltransf_12
Aldo_ket_red
DUF300
MFS_1
Zn_clus
MFS_1
MFS_1
FAD_binding_4
Anp1
adh_short
MFS_1
MFS_1
Fungal_trans
WD40
WD40
SH3_1
Pyridoxal_deC
MFS_1
Thioredoxin
Methyltransf_12
Ribosomal_S8e
TIM
Fungal_trans
CASP_C
PMI_typeI
Glyco_hydro_28
Homoserine_dh
Zn_clus
WD40
PHD
Glyco_transf_5
Cation_efflux
adh_short
AAA
PFAM_DESCRIPTION
Flavin containing amine oxidoreductase
F-box domain
D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase
Phosphotransferase enzyme family
DegT/DnrJ/EryC1/StrS aminotransferase family
XAP5 protein
Mechanosensitive ion channel
RTA1 like protein
WW domain
Phosphoglycerate mutase family
Uncharacterized ACR, YdiU/UPF0061 family
Ubiquitin carboxyl-terminal hydrolase
3-hydroxyacyl-CoA dehydrogenase, C-terminal domain
CFEM domain
SPRY domain
4'-phosphopantetheinyl transferase superfamily
Glutamine synthetase, catalytic domain
Tubulin-tyrosine ligase family
Prenyltransferase and squalene oxidase repeat
Tannase and feruloyl esterase
Polyprenyl synthetase
RTA1 like protein
Mandelate racemase / muconate lactonizing enzyme, C-terminal dom
Tubulin binding cofactor C
GDP dissociation inhibitor
Biotin-requiring enzyme
KR domain
Saccharopine dehydrogenase
Glycosyl transferases group 1
F-box domain
Transferase family
PPR repeat
Major intrinsic protein
Glutathione S-transferase, C-terminal domain
CRAL/TRIO domain
Dynamin family
ABC transporter
HMG (high mobility group) box
Ubiquinone biosynthesis protein COQ7
Cytochrome P450
Pectate lyase
Coenzyme Q (ubiquinone) biosynthesis protein Coq4
haloacid dehalogenase-like hydrolase
D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase
K+ potassium transporter
ribonuclease
AMMECR1
Amino acid permease
Domain of unknown function
FAD binding domain
Anp1
SH3 domain
Thioredoxin
Ribosomal protein S8e
Triosephosphate isomerase
CASP C terminal
Phosphomannose isomerase type I
Homoserine dehydrogenase
PHD-finger
Starch synthase catalytic domain
Cation efflux family
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500

'
LOCUS
AO090701000750
AO090010000350
AO090102000018
AO090003000055
AO090113000007
AO090020000072
AO090701000576
AO090011000219
AO090038000561
AO090005000060
AO090012000633
AO090009000079
AO090038000303
AO090010000526
AO090009000130
AO090038000504
AO090012000233
AO090011000022
AO090012000947
AO090012000823
AO090020000258
AO090020000650
AO090009000457
AO090102000173
AO090701000642
AO090038000206
AO090001000279
AO090009000472
AO090038000182
AO090003000575
AO090026000639
AO090011000344
AO090003001502
AO090124000033
AO090003001146
AO090005000879
AO090701000440
AO090010000531
AO090011000126
AO090003000334
AO090011000452
AO090701000806
AO090120000003
AO090701000146
AO090001000174
AO090003001529
AO090003000457
AO090011000716
AO090026000652
AO090003000793
AO090701000751
AO090003001503
AO090010000477
AO090023000217
AO090003000402
AO090102000065
AO090009000495
AO090001000678
AO090003001092
AO090003001277
AO090120000006
AO090020000336
AO090026000379
AO090102000050
AO090010000338
AO090026000820
AO090102000053
AO090138000033
AO090001000544
AO090010000031
AO090003000489
AO090206000119
AO090020000503
AO090020000618
AO090120000485
AO090701000398
AO090023000073
AO090701000303
AO090038000090
AO090023000531
AO090010000344
AO090003001077
AO090005000162
AO090026000365
AO090003001108
AO090011000404
AO090120000287
AO090020000502
AO090023000021
AO090701000827
AO090001000260
AO090102000422
AO090020000005
AO090003000648
AO090009000559
AO090005000602
AO090103000216
AO090020000489
AO090011000876
AO090003000289
Coef
F.p.value
-0.6707
0.02997
-0.8802
0.03000
-1.2969
0.03005
-0.6417
0.03012
-1.1313
0.03012
-1.6104
0.03032
-0.5624
0.03046
-0.5512
0.03047
0.8712
0.03048
-1.6889
0.03057
-0.788
0.03062
-0.5888
0.03085
-1.1138
0.03088
-0.8692
0.03099
-0.5224
0.03108
-0.8278
0.03134
0.5596
0.03143
-0.7652
0.03152
0.909
0.03154
-0.6811
0.03163
-0.6469
0.03178
1.5041
0.03180
-0.9438
0.03181
-2.1691
0.03192
-1.3797
0.03194
-0.8648
0.03211
-0.5166
0.03216
-0.513
0.03216
0.6476
0.03218
1.0834
0.03219
-1.4447
0.03229
-0.6151
0.03229
2.5753
0.03233
0.5565
0.03233
-0.7294
0.03236
-0.5694
0.03253
-0.7145
0.03253
-0.6521
0.03253
0.8478
0.03261
0.5955
0.03266
0.9047
0.03267
-0.79
0.03274
0.9373
0.03275
-0.5298
0.03275
0.5949
0.03277
0.4899
0.03291
-2.6235
0.03291
-1.4537
0.03292
-0.5042
0.03306
0.8284
0.03311
-1.4788
0.03315
3.2559
0.03320
-1.3487
0.03325
-0.6822
0.03331
-0.6955
0.03333
-2.4123
0.03338
-0.8736
0.03341
-0.77
0.03357
-0.4854
0.03367
-0.5293
0.03385
3.0559
0.03388
-1.401
0.03392
0.5592
0.03395
-0.6195
0.03396
-0.5529
0.03403
-1.3229
0.03416
0.7003
0.03417
1.0827
0.03421
-1.0872
0.03428
0.5604
0.03431
-0.4843
0.03434
-0.8295
0.03443
-1.2117
0.03444
-0.6604
0.03476
-0.5678
0.03481
-1.4412
0.03485
2.1132
0.03502
0.5157
0.03505
0.5214
0.03508
-0.6198
0.03513
0.5602
0.03519
0.5893
0.03524
-0.7165
0.03531
-0.5719
0.03540
-0.952
0.03550
0.6869
0.03558
-1.2625
0.03573
-1.6761
0.03579
-2.3076
0.03598
-1.276
0.03600
1.996
0.03601
0.5597
0.03602
-0.7577
0.03613
-0.8853
0.03616
-1.3993
0.03630
-1.3905
0.03634
-2.102
0.03634
-0.496
0.03637
-1.6976
0.03639
-1.1585
0.03688
PFAM_NAME
Init_tRNA_PT
MFS_1
FAD_binding_7
Enolase_C
GMC_oxred_C
Glyoxalase
Glyco_transf_8
Fungal_trans
C2
AA_permease
Iso_dh
ADH_N
ABC2_membrane
Aa_trans
p450
Pectate_lyase
2-Hacid_dh_C
AOX
VWA
Sds3
Pyr_redox_2
BBE
STAS
DAO
Pkinase
p450
Ketoacyl-synt_C
Bac_rhamnosid
PALP
MFS_1
Dioxygenase_C
Helicase_C
Glyco_transf_5
LysM
Fe-S_biosyn
F-box
MFS_1
Transp_cyt_pur
Na_Ca_ex
Transferase
Chitin_synth_1
CinA
Aldo_ket_red
Mak16
Glyco_hydro_88
COesterase
DUF1960
DUF833
Myb_DNA-binding
Formyl_trans_C
Aldo_ket_red
Glyco_transf_5
UPF0057
DNA_pol_delta_4
Pkinase
Aldedh
Cyclin
YL1_C
Mannosyl_trans2
Sugar_tr
Peptidase_A4
NIPSNAP
Condensation
AAA
Fungal_trans
Trehalose_PPase
Abhydrolase_4
Glycos_transf_2
Glyco_hydro_3_C
Pec_lyase_C
Methyltransf_12
WD40
KR
IDO
Snf7
Helicase_C
p450
MFS_1
Sugar_tr
TauD
Pyridoxal_deC
Aminotran_1_2
Evr1_Alr
MFS_1
C2
COesterase
DSPc
KR
Methyltransf_12
adh_short
p450
Ank
Peptidase_C54
Pex19
adh_short
Peptidase_C1_2
GMC_oxred_C
PRP38
Malic_M
Rad4
PFAM_DESCRIPTION
Initiator tRNA phosphoribosyl transferase
FAD binding domain of DNA photolyase
Enolase, C-terminal TIM barrel domain
GMC oxidoreductase
C2 domain
Amino acid permease
Isocitrate/isopropylmalate dehydrogenase
Alcohol dehydrogenase GroES-like domain
Transmembrane amino acid transporter protein
Cytochrome P450
Pectate lyase
Alternative oxidase
von Willebrand factor type A domain
Sds3-like
STAS domain
Cytochrome P450
Bacterial alpha-L-rhamnosidase
Dioxygenase
LysM domain
Iron-sulphur cluster biosynthesis
F-box domain
Permease for cytosine/purines, uracil, thiamine, allantoin
Transferase family
Chitin synthase
Competence-damaged protein
Mak16 protein
Glycosyl Hydrolase Family 88
Carboxylesterase
Myb-like DNA-binding domain
Formyl transferase, C-terminal domain
Uncharacterized protein family UPF0057
DNA polymerase delta, subunit 4
Cyclin
YL1 nuclear protein C-terminal domain
Peptidase A4 family
NIPSNAP
Condensation domain
Trehalose-phosphatase
TAP-like protein
Glycosyl hydrolase family 3 C terminal domain
Pectate lyase
KR domain
Indoleamine 2,3-dioxygenase
Snf7
Cytochrome P450
Taurine catabolism dioxygenase TauD, TfdA family
Erv1 / Alr family
C2 domain
Carboxylesterase
Dual specificity phosphatase, catalytic domain
KR domain
Cytochrome P450
Ankyrin repeat
Peptidase family C54
Pex19 protein family
Peptidase C1-like family
GMC oxidoreductase
PRP38 family
Malic enzyme, NAD binding domain
DNA repair protein Rad4
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
596
597
598
599
600

)*)

'
LOCUS
AO090005000818
AO090003001294
AO090005001525
AO090011000646
AO090001000113
AO090023000371
AO090701000260
AO090009000299
AO090038000462
AO090012000003
AO090005000426
AO090701000199
AO090020000049
AO090009000628
AO090102000309
AO090003001099
AO090005000959
AO090005000075
AO090023000082
AO090003000024
AO090003001387
AO090701000831
AO090005000809
AO090005001636
AO090010000406
AO090012000592
AO090020000567
AO090003001315
AO090005001066
AO090003001542
AO090701000110
AO090005001454
AO090102000538
AO090001000710
AO090020000665
AO090701000685
AO090003000984
AO090103000099
AO090012000264
AO090026000382
AO090011000108
AO090701000280
AO090005001467
AO090011000917
AO090009000114
AO090026000037
AO090701000175
AO090003000832
AO090005000243
AO090020000603
AO090010000219
AO090023000786
AO090011000712
AO090023000136
AO090009000286
AO090010000534
AO090005001116
AO090005000972
AO090012000837
AO090001000684
AO090020000352
AO090701000046
AO090010000014
AO090020000571
AO090026000507
AO090003001412
AO090011000722
AO090005001209
AO090011000659
AO090026000629
AO090010000164
AO090012000670
AO090011000167
AO090701000002
AO090026000607
AO090102000621
AO090020000704
AO090005001228
AO090038000234
AO090701000656
AO090020000572
AO090010000239
AO090023000518
AO090012000768
AO090138000154
AO090206000117
AO090023000772
AO090038000505
AO090009000015
AO090012000942
AO090124000083
AO090026000062
AO090026000712
AO090003000252
AO090701000599
AO090020000495
AO090005000808
AO090005001510
AO090103000494
AO090023000927

)*=
Coef
F.p.value
1.0589
0.03697
-0.6283
0.03700
-1.4232
0.03707
0.7716
0.03714
0.9477
0.03754
0.9547
0.03764
0.5336
0.03765
-0.7075
0.03770
0.6885
0.03773
-0.5404
0.03787
-0.5771
0.03789
-0.9719
0.03791
0.5009
0.03791
-0.8721
0.03803
-1.3158
0.03806
-0.7664
0.03809
-0.4858
0.03810
0.6379
0.03820
0.9546
0.03821
-2.1402
0.03834
0.7153
0.03841
-1.4522
0.03846
-0.9301
0.03846
0.6108
0.03849
0.5815
0.03865
-0.5349
0.03871
0.5863
0.03872
0.7742
0.03879
-0.4997
0.03889
-0.5728
0.03896
1.3641
0.03898
-0.5509
0.03900
-1.1072
0.03903
-0.4786
0.03910
2.6576
0.03913
-1.349
0.03915
-3.5383
0.03920
-0.8618
0.03925
-1.0878
0.03939
0.473
0.03948
-1.2931
0.03955
-0.5727
0.03971
-0.6143
0.03973
-0.5971
0.03976
0.4887
0.03989
1.1507
0.03989
-0.4666
0.03999
0.4782
0.04002
-0.6377
0.04016
0.535
0.04042
0.9667
0.04044
-1.258
0.04046
-0.9046
0.04063
-1.4426
0.04065
-0.6466
0.04065
0.9258
0.04077
0.7982
0.04084
0.5599
0.04085
-0.7203
0.04095
0.5606
0.04101
-0.7579
0.04101
-0.9604
0.04107
0.5214
0.04108
-1.931
0.04109
-1.4573
0.04113
-1.5055
0.04121
-0.7205
0.04122
-1.1825
0.04131
-0.7909
0.04154
-0.5853
0.04190
-0.612
0.04195
-1.6865
0.04203
0.4555
0.04217
-0.7118
0.04223
-1.0517
0.04233
-1.0093
0.04235
0.5041
0.04238
-0.7494
0.04239
0.5473
0.04240
0.5077
0.04252
0.4837
0.04254
-0.5263
0.04268
-1.062
0.04274
0.8069
0.04277
-0.5756
0.04293
-1.2858
0.04296
-0.5353
0.04298
0.825
0.04313
0.9793
0.04325
-0.5412
0.04329
0.7673
0.04331
-0.4628
0.04332
0.6306
0.04335
-0.4671
0.04337
-0.7558
0.04345
-1.2402
0.04347
-1.5799
0.04349
-0.4684
0.04359
-3.6999
0.04365
1.4245
0.04366
PFAM_NAME
Asparaginase
Zn_clus
Memo
NDT80_PhoG
GDPD
Acyl_transf_3
Aegerolysin
GATA
MFS_1
Glyco_hydro_3_C
Methyltransf_12
DUF167
Isochorismatase
MFS_1
Methyltransf_12
Ald_Xan_dh_C
WD40
MFS_1
NAD_binding_4
WW
MFS_1
Acyl_transf_1
WD40
zf-C3HC4
Mito_carr
efhand
AA_permease
Lipase_3
ABC_tran
MFS_1
GIDA
NUDIX
Helicase_C
Hexokinase_2
Fungal_trans
SE
Aldedh
MFS_1
LuxC
TRI5
Abhydrolase_1
UBA_3
PWI
AMP-binding
Ldl_recept_b
MFS_1
MmgE_PrpD
MFS_1
Thioredoxin
FGGY_C
ABC2_membrane
NPD
p450
HEAT
DUF1771
Peptidase_S10
Adap_comp_sub
UDPG_MGDP_dh_C
UQ_con
RRM_1
HET
2-Hacid_dh_C
ACOX
CN_hydrolase
MFS_1
Amidase
Usp
Clp_N
PGI
Yippee
Ank
CsbD
DUF1212
Asp
GATase
HSP90
4HBT
AMP-binding
Alpha-amylase
adh_short
ABC_tran
Fungal_trans
NAD_binding_2
GATA
HET
Methyltransf_12
cobW
Acetyltransf_1
Aa_trans
Abhydrolase_1
Ketoacyl-synt_C
PALP
MHYT
Abhydrolase_1
tRNA-synt_1c_C
Biotin_carb_C
WD40
Oxysterol_BP
p450
adh_short
PFAM_DESCRIPTION
Asparaginase
Memo-like protein
NDT80 / PhoG like DNA-binding family
Glycerophosphoryl diester phosphodiesterase family
Acyltransferase family
Aegerolysin
GATA zinc finger
Glycosyl hydrolase family 3 C terminal domain
Uncharacterised ACR, YggU family COG1872
Isochorismatase family
Aldehyde oxidase and xanthine dehydrogenase, a/b hammerhead dom
WW domain
Acyl transferase domain
Zinc finger, C3HC4 type (RING finger)
Mitochondrial carrier protein
EF hand
Amino acid permease
Lipase (class 3)
ABC transporter
Glucose inhibited division protein A
NUDIX domain
Hexokinase
Squalene epoxidase
Acyl-CoA reductase (LuxC)
Trichodiene synthase (TRI5)
Fungal ubiquitin-associated domain
PWI domain
AMP-binding enzyme
Low-density lipoprotein receptor repeat class B
MmgE/PrpD family
Thioredoxin
2-nitropropane dioxygenase
Cytochrome P450
HEAT repeat
Adaptor complexes medium subunit family
UDP-glucose/GDP-mannose dehydrogenase family, UDP binding dom
Acyl-CoA oxidase
Carbon-nitrogen hydrolase
Amidase
Universal stress protein family
Clp amino terminal domain
Phosphoglucose isomerase
Ankyrin repeat
CsbD-like
Glutamine amidotransferase class-I
Hsp90 protein
Thioesterase superfamily
AMP-binding enzyme
Alpha amylase, catalytic domain
ABC transporter
NAD binding domain of 6-phosphogluconate dehydrogenase
GATA zinc finger
CobW/HypB/UreG, nucleotide-binding domain
Acetyltransferase (GNAT) family
Transmembrane amino acid transporter protein
Bacterial signalling protein N terminal repeat
tRNA synthetases class I (E and Q), anti-codon binding domain
Biotin carboxylase C-terminal domain
Oxysterol-binding protein
Cytochrome P450
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
622
623
624
625
626
627
628
629
630
631
632
633
634
635
636
637
638
639
640
641
642
643
644
645
646
647
648
649
650
651
652
653
654
655
656
657
658
659
660
661
662
663
664
665
666
667
668
669
670
671
672
673
674
675
676
677
678
679
680
681
682
683
684
685
686
687
688
689
690
691
692
693
694
695
696
697
698
699
700

'
LOCUS
AO090038000302
AO090011000220
AO090012000813
AO090701000093
AO090003001027
AO090003000594
AO090010000533
AO090005000166
AO090005001512
AO090701000526
AO090003001362
AO090020000611
AO090005000650
AO090113000104
AO090120000293
AO090023000591
AO090003001243
AO090138000024
AO090005001479
AO090005001009
AO090103000442
AO090701000518
AO090003001501
AO090102000117
AO090010000633
AO090001000077
AO090005001564
AO090012000397
AO090010000574
AO090003000526
AO090005000216
AO090003000246
AO090023000444
AO090011000600
AO090012000788
AO090120000492
AO090009000066
AO090120000377
AO090005000613
AO090005001128
AO090020000170
AO090020000096
AO090124000004
AO090026000225
AO090012000476
AO090012000143
AO090005000805
AO090102000184
AO090003000896
AO090003000548
AO090001000124
AO090206000020
AO090003000693
AO090701000525
AO090020000536
AO090003001336
AO090011000447
AO090010000340
AO090102000377
AO090701000583
AO090012000721
AO090102000135
AO090012000754
AO090005000355
AO090010000492
AO090005000577
AO090120000463
AO090001000617
AO090009000209
AO090120000165
AO090020000137
AO090003000748
AO090103000407
AO090011000676
AO090012000119
AO090038000623
AO090020000634
AO090011000215
AO090020000203
AO090026000516
AO090010000237
AO090001000350
AO090012000807
AO090003000942
AO090701000172
AO090010000129
AO090005001079
AO090012000219
AO090103000002
AO090038000350
AO090012000029
AO090012000843
AO090020000561
AO090020000508
AO090020000531
AO090026000627
Coef
F.p.value
-0.9215
0.04400
0.4447
0.04401
0.4677
0.04409
0.6604
0.04410
-0.4953
0.04410
-0.6694
0.04410
-0.7346
0.04412
-0.7526
0.04426
-0.5217
0.04431
1.717
0.04444
0.4502
0.04445
-0.4773
0.04454
-0.5555
0.04464
-0.4503
0.04472
-0.8474
0.04479
0.5777
0.04479
-0.9045
0.04484
-1.6036
0.04510
-0.7588
0.04532
0.7068
0.04538
-0.4498
0.04539
-0.443
0.04539
2.0936
0.04539
-0.6286
0.04543
-0.7147
0.04544
-0.5683
0.04547
-0.6729
0.04554
2.8423
0.04554
1.8176
0.04559
0.5362
0.04560
0.5151
0.04562
0.49
0.04563
0.5522
0.04570
0.4588
0.04573
-0.4979
0.04587
-0.9833
0.04590
1.4099
0.04600
1.8606
0.04612
-0.6659
0.04612
-0.5075
0.04617
-0.609
0.04625
-0.5596
0.04647
-0.9553
0.04653
-0.503
0.04664
-0.817
0.04666
3.9351
0.04669
-1.1444
0.04671
0.7718
0.04676
-0.4495
0.04682
-1.0999
0.04691
0.5571
0.04697
-0.7266
0.04701
-0.8677
0.04711
2.347
0.04713
0.4387
0.04717
-2.051
0.04725
-0.6671
0.04726
0.7638
0.04737
-1.3537
0.04742
-0.6719
0.04781
-0.4715
0.04802
-1.0092
0.04809
-1.0297
0.04840
1.0186
0.04843
-0.7278
0.04850
0.6938
0.04853
-0.6527
0.04857
-3.4842
0.04858
1.0716
0.04868
-0.7882
0.04869
0.5648
0.04871
-0.4662
0.04876
-0.8702
0.04876
0.456
0.04877
-0.4777
0.04890
-0.4321
0.04893
-0.4823
0.04896
-3.1095
0.04898
-0.5621
0.04911
-0.645
0.04911
-0.4973
0.04915
-0.5935
0.04939
-0.451
0.04947
-2.0179
0.04961
-0.6434
0.04969
0.4425
0.04982
0.4814
0.04988
-0.85
0.04990
-0.5199
0.04996
-0.7544
0.05002
-0.7426
0.05002
0.5792
0.05004
0.4772
0.05006
0.5176
0.05017
0.4739
0.05029
-1.4048
0.05043
PFAM_NAME
DUF1604
Fungal_trans
Glyco_hydro_72
Ribosomal_S9
LRR_1
Glycos_transf_2
NmrA
MFS_1
Oxysterol_BP
KR
Zn_clus
MFS_1
AA_permease
adh_short
AAA_5
Fungal_trans
DUF77
HisKA
Met_10
Mtc
p450
HATPase_c
Glyco_transf_5
NOC3p
Cupin_2
S1-P1_nuclease
Iso_dh
2OG-FeII_Oxy
adh_short
TauD
NAD_binding_4
Pkinase
PP-binding
Aminotran_4
Band_7
Pro_isomerase
Aldolase_II
AMP-binding
HEAT
Pkinase
TPR_2
Dabb
adh_short
Sec1
TFIID-18kDa
DUF895
Helicase_C
RTA1
PSP1
Methyltransf_12
MFS_1
Na_Ca_ex
Asp
FAD_binding_4
adh_short
Acid_phosphat_A
AMP-binding
Amino_oxidase
Sulfatase
PALP
Palm_thioest
MFS_1
Fungal_trans
TRP
DPBB_1
Myosin_TH1
MFS_1
BBE
WSC
FMN_dh
Fungal_trans
GFO_IDH_MocA
UQ_con
Pec_lyase_C
F420_oxidored
Malic_M
AhpC-TSA
HLH
DFP
E1_DerP2_DerF2
Fungal_trans
Glyco_hydro_7
XPG_I
Methyltransf_12
Aldedh
Bac_luciferase
ADH_zinc_N
Biotin_lipoyl
Pro_isomerase
Ank
Methyltransf_12
ChAPs
Opi1
Lipase_GDSL
DUF1479
Yippee
PFAM_DESCRIPTION
Glycolipid anchored surface protein (GAS1)
Ribosomal protein S9/S16
Leucine Rich Repeat
NmrA-like family
Oxysterol-binding protein
KR domain
Amino acid permease
Domain of unknown function DUF77
His Kinase A (phosphoacceptor) domain
Met-10+ like-protein
Tricarboxylate carrier
Cytochrome P450
Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Nucleolar complex-associated protein
Cupin domain
S1/P1 Nuclease
Isocitrate/isopropylmalate dehydrogenase
2OG-Fe(II) oxygenase superfamily
Taurine catabolism dioxygenase TauD, TfdA family
Aminotransferase class IV
SPFH domain / Band 7 family
Class II Aldolase and Adducin N-terminal domain
AMP-binding enzyme
HEAT repeat
Stress responsive A/B Barrel Domain
Sec1 family
Transcription initiation factor IID, 18kD subunit
Eukaryotic protein of unknown function (DUF895)
RTA1 like protein
PSP1 C-terminal conserved region
FAD binding domain
Histidine acid phosphatase
AMP-binding enzyme
Flavin containing amine oxidoreductase
Sulfatase
Palmitoyl protein thioesterase
Transient receptor potential (TRP) ion channel
Rare lipoprotein A (RlpA)-like double-psi beta-barrel
Myosin tail
WSC domain
Oxidoreductase family, NAD-binding Rossmann fold
Pectate lyase
NADP oxidoreductase coenzyme F420-dependent
Malic enzyme, NAD binding domain
AhpC/TSA family
Helix-loop-helix DNA-binding domain
DNA / pantothenate metabolism flavoprotein
ML domain
XPG I-region
Luciferase-like monooxygenase
Biotin-requiring enzyme
Ankyrin repeat
ChAPs (Chs5p-Arf1p-binding proteins)
Transcription factor Opi1
GDSL-like Lipase/Acylhydrolase
701
702
703
704
705
706
707
708
709
710
711
712
713
714
715
716
717
718
719
720
721
722
723
724
725
726
727
728
729
730
731
732
733
734
735
736
737
738
739
740
741
742
743
744
745
746
747
748
749
750
751
752
753
754
755
756
757
758
759
760
761
762
763
764
765
766
767
768
769
770
771
772
773
774
775
776
777
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Appendix II

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CMB is an Engineering Center of Excellence funded by the Danish Research Agency. It is a

collaboration between an acknowledged research manager, his/her institute and university,
and the Research Agency. An Engineering Center of Excellence is a research institute of
first-class quality with tradition for cooperation with industry.
Study of Mass Transfer inViscous Fermentations

Study of Mass Transfer in Viscous

Fermentations
David Kold
Ph.D. Thesis
April 2010
Technical University of Denmark
Building 223
DK-2800 Kgs. Lyngby
Denmark
Phone:
+45 4525 2690
Fax:
+45 4588 4148
www.cmb.dtu.dk
David Kold
ISBN-nr: 9788791494918


Study of Mass Transfer in Viscous Fermentations

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CMB is an Engineering Center of Excellence funded by the Danish Research Agency.

Study of Mass Transfer inViscous Fermentations

Study of Mass Transfer in Viscous

+45 4525 2690

+45 4588 4148

Center for Microbial Biotechnology

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Frontpage: Broth from two Aspergillus oryzae fermentations.

Copyright David Kold 2010

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