1.

Title, Groups member names and date

2. Introduction (Background)-- include information on bacteria structure and function,
antibiotic resistance and culturing bacteria- Quinn
Bacteria can be found in five different shapes that signify what type of bacteria it is.
Rod-Shaped(Baccilus), Spherical(coccus), Curved(Bibrio, spirochaete), Squareshaped(Arcula) and Star-shaped(Stella).
Bacteria is also arranged in either a pair(escherichia), chain(streptococcus) or a
cluster(staphylococcus).
Bacteria contains a cell wall to protect cells against osmotic shock, a membrane to
regulate transportation in and out of the cells. A chromosome is the genome while the
Plasmid contains genetic information like resistances to antibiotics and tolerance of
toxins. Ribosomes take part in protein synthesis. Flagella helps the cells move. Body is
the storage of cells. Pili is how the bacteria mate. Endospore is heat resistant and helps
the bacteria survive. (http://www.fastbleep.com/biology-notes/35/112/654)
Growing bacteria is as easy as it sounds. You simply need a product and if you keep it
in a incubator, soon enough bacteria will grow.
3. Abstract (Purpose)- What are you testing? Why is this important? How does it relate
to the Zombie Apocalypse?
We are testing the effectiveness of different natural antibiotics to disperse the E.Coli
bacteria/virus that is making the people zombified. Our antibiotics are coconut oil, tea
tree oil, clove, cinnamon, oregano, and honey. We tested all of these with the hope that
a couple would be able to serve as an antibiotic for the virus. This is important because
we need to know what natural resources we can use in order to control the Zombie
Apocalypse. The Zombie Apocalypse is getting out of hand and if we dont test these
natural antibiotics, then it may be too late to control the zombies.
4. Methods- Materials and Procedure (pictures are helpful here!)
Materials:
Sterile loop
E.Coli bacteria
2 Agar dishes
Parafilm
Paper cutouts for soaking the natural antibiotic in
teaspoon of tea tree oil, clove, cinnamon, oregano, honey, coconut oil
Sanitation supplies (wipes and cleaning spray)
5. Results- Take pictures, describe plates, measure the zone of inhibition (data table),
gram stain results-Maegan
Pictures:
Clove was the only one that remotely worked out of our six antibiotics.

Results- Take pictures, describe plates, measure the zone of inhibition (data table),
gram stain results
Pictures:
Clove (2) was the only one that remotely worked out of our six antibiotics.

Plate 1: Coconut oilthis area on the plate shows a very large expansion of the
bacteria around the whole paper because the coconut did not work at all. If anything it
grew more of the bacteria, almost as much as the control group.
Plate 2: Clovethe clove was the only natural antibiotic we tested that had any
significant change to the growing bacteria. There was about a 1mm inhibition size,
which was remotely small comparatively to another stronger antibiotic that we couldve
used.
Plate 3: Tea Treethis area on the plate didnt grow as many bacteria around the paper
sample, but it didnt make the bacteria disperse either. There werent too many bacteria
only a thin layer, about half of the control group.
Plate 4: Oreganothere was a thick area of bacteria around the paper sample for
oregano. It was also tinted orange, which may say that it was contaminated.
Nonetheless bacteria grew and were not dispersed.
Plate 5: Cinnamonmany bacteria grew in this area, closely related to the control group
of no natural antibiotic. The paper for this antibiotic had large coverage of the bacteria.
Plate 6: Honeylarge bacterium can be seen around the honey which concludes it did
not work very well. This specific area is worse than the control on the same plate with a
thick white layer of bacteria around the paper sample.
Gram Stain Results

E.Coli is gram positive according to our results. The gram positive bacteria means that it
has a thick cell wall.

6. Conclusion and Recommendations- What happened? Did any of the test natural
antibiotics work? Sources of Error (3)? What do you recommend as next steps or future
research?-Megan
In our experiment to try and find a cure, we unfortunately concluded no natural
antibiotics that work, but our scientists are hard at work to find a cure.
We grew the bacteria and tried to find the initial cause of the disease.
Our margin for error could have been prevented through using more precautions when
we plated our bacteria and when we would open and close the plates.
When finding a cure for the antibiotics, we could have used a more concentrated
solution of the disks on the plate so that they were more efficient and effective at killing
and stopping the disease from spreading.
In our future research, we are going to keep trying to find as cure as that is the most
important step for containing the outbreak at Monarch University.
We are going to develop a vaccination that can combat against the disease and prevent
it from happening in the future. With further research we want to develop antibodies that
will be injected into the bloodstream so that the disease can subside more quickly.
7. Zombie Apocalypse Containment- Research how to contain a disease and give an
outline as to what you recommend for quarantine procedures. You may include a
video(s) to educate people at Monarch University and Louisville about the Zombie
disease (symptoms, how it is spread, etc) and on quarantine procedures. You may be
creative and interview people to add to this section...in other words, have fun with this
part!-Morgan
How is this disease Spread?
Students fell sick after eating the ice cream at the bowling alley, so it might be due to
food contamination
http://www.niaid.nih.gov/topics/foodborne/pages/default.aspx
It is not known if the disease has the capability to evolve other modes of infection, so it
is important to take all precautionary measure to ensure public safety.

 What are the symptoms

Hard to tell if you are sick, but if you feel like you have gained superpowers or if other
around you start to act strange and talk fast, you must go find a doctor as soon as
possible
Trust family members and close friends, if they believe that you are sick take a
precautionary measure and go to the doctor to be evaluated.
How to quarantine
The CDC defines two steps in order to reduce the spread of disease
Isolation separates sick people with a contagious disease from people who are not
sick.
Quarantine separates and restricts the movement of people who were exposed to a
contagious disease to see if they become sick.
The general public should avoid the bowling alley ice cream, until researchers have a
chance to thoroughly investigate the source.
Avoid contact with those who may have been infected and if you experience symptoms,
admit yourself to a hospital and avoid contact with others

http://www.cdcfoundation.org/content/how-cdc-saves-lives-controlling-real-globaldisease-outbreaks

Procedure and data table:

How are we going to test if a natural antibiotic will be effective?
1.
2.
3.
4.
a.
5.
6.

Obtain an agar disk that has either E.coli or yogurt bacteria on it

Create solutions with 3 pipettes of distilled water and 2 parts natural antibiotic
Soak 5 different paper disks in various solutions for at least 10 minutes
Remove the paper disk from its solution and place on agar dish
Do not let the paper disk drip onto the agar plate
Place the plate in the incubator and let the bacteria grow for at least 24 hours
Remove the plate from the incubator and observe and record the field on inhibition

Natural Antibiotic

Prediction:
Will it work?

Observations

Estimate field of
inhibition size

Coconut oil

Yes

No field of inhibition

Clove

No

Very small field of

inhibition

1 mm

Tea Tree oil

Yes

No field of inhibition

Oregano oil

Yes

No field of inhibition

Cinnamon

Yes

No field of inhibition

Honey

Yes

No field of inhibition

Picture Bibliography:
http://www.aliexpress.com/store/product/ZOMBIE-OUTBREAK-RESPONSE-TEAMBIOHAZARD-SYMBOL-VINYL-DECAL-CAR-WINDOWSTICKER/126209_1957583335.html
http://www.amazon.com/LAMINATED-Zombie-Research-FacilityPoster/dp/B00A809X6Y
http://darthbyron.deviantart.com/art/Biohazard-Zombie-zone-175844488
https://www.rt.com/uk/200827-biohazard-suits-ebola-sold/
http://www.bvsd.org/high/monarch/pages/monarch.aspx
https://www.pinterest.com/explore/plants-vs-zombies/
http://www.polyvore.com/neon_green_ice-cream_edited_peace_love_pink/thing?
id=13356947
http://www.referenceforbusiness.com/business-plans/Business-Plans-Volume07/Bowling-Alley.html
https://en.wikipedia.org/wiki/Bacterial_lawn
http://www.alibaba.com/product-detail/Supplier-of-Clove-from-India_167390917.html
http://blog.heartland.org/2014/01/antibiotic-surprise/
http://myphonehelpdesk.com/Support-for-Microsoft-Windows/windows-7-updateproblems/how-to-fix-windows-7-update-error-0x80070002/
https://www.pinterest.com/pin/385480049327405865/

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