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Janakorn
Chummuang (First)
Boondaree
Daophiphatchai (Pleng)
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Kanwara
Boonchai (Praewa)
Nuttacha
Itthpanyavorakul (Fah)
11 - 03
Introduction:
When we talk about DNA, the first thing that comes into mind is that DNA is related to
genetic or one of genetic materials that is essential for our lives or other living organisms.
Without DNA, we will not be able to function the body or to survive. The word DNA stands for
deoxyribonucleic acid, which occurs in the form of double helix. DNA shape is two spirals that
twist around each other; each of them is the backbone of the DNA which made up of
phosphates and sugar. There are four bases in DNA such as adenine (A), thymine (T), guanine
(G) and cytosine (C), where the spirals are connected. Each of the bases pair up together:
adenine with thymine and guanine with cytosine. The functions of the DNA is to store genetic
information of living organisms in order survive, grow, and function all processes inside the
body. In eukaryotes, DNA is stored in the nucleus, and for prokaryotes is in the cells cytoplasm.
Extraction is the process of helping to take the important part of the substance out by
using force or effort to proceed. For the benefits of DNA extraction include: plants, animals,
pharmaceuticals, medical diagnosis, and genetic verification. To get the DNA from a cell, the
scientists will need some materials to isolate the DNA. During the DNA extraction, a detergent
will cause a cell to pop open or become lysed, so that the DNA can be released into the
solution, then alcohol is added to the solution to cause the DNA to precipitate out.
For this experiment, the extraction process is collaborated with DNA in living organisms.
Strawberry represents a specimen in this experiment instead of other living organisms due to its
eight copies of genome characteristic.The purpose of this experiment was to complete the tasks
given by using our knowledge about DNA structure, interactions, and understand the properties
of the materials in order to complete the process of extraction. It also showed how DNA can be
extracted from organisms by isolating the DNA from strawberry to be seen with naked eye, and
how organisms develop themselves and function due to the DNA.
Materials:
- 3 strawberries
Petri dish
Stirring rod
Tweezers
Spatula
Zip-lock bag
Cheesecloth
Method:
1. Put the bottle of isopropyl alcohol in the freezer
2. Measure 18 mL of water with 50 mL graduated cylinder, then pour it into the
beaker.
3. Pour dish detergent solution into the same beaker
4. Add spatula of salt into the same beaker
5. Mix the solution with stirring rod
6. Pour the solution from the beaker into the zip-lock bag that contained strawberry.
7. Seal the bag
8. Mix the strawberry with the solution until it becomes small pieces.
9. After mixing, pour it through cheesecloth into a 250 mL beaker.
10. Pour the solution into the 50 mL beaker and add 10 mL of isopropyl alcohol into
the same beaker.
11. Use the tweezers to remove the white substance on top of the solution.
12. Then place the substance onto the petri dish.
13. Record the result.
Results:
Picture 1 - The white goo of DNA starting to separate from its solution as layers formed.
Conclusion:
DNA of the strawberry is extracted and found to be mucus and pink. In this experiment,
we learnt the simple process to extract the DNA of Strawberries.
References:
- What is biotechnology. (N.d). DNA extraction. (online). retrieved May 13th 2015 ,
from: http://www.whatisbiotechnology.org/science/extraction
- U.S. National Library of Medicine. (2015). what is DNA?. (online). retrieved May
13th 2015 , from: http://ghr.nlm.nih.gov/handbook/basics/dna
- Virginia department of education. (2012). DNA extraction from strawberries.
(online). retrieved May 13th 2015, from :
http://www.doe.virginia.gov/testing/sol/standards_docs/science/2010/lesson_plans/life_s
ci/ecosystems/sess_LifeSci-12ae.pdf
- Ray, C. (2008). DNA Parade. (online). retrieve May 13th 2015, from:
http://www.nytimes.com/2008/06/03/science/03qna.html?_r=0
- Pease,R. (2012). DNA imaged with electron microscope for the first time.
(online). retrieve May 13th 2015, from: http://www.newscientist.com/article/dn22545-dnaimaged-with-electron-microscope-for-the-first-time.html#.VVMsidOqqko
- Adam-Day,S.(2015). Magnification. (online).retrieve May 13th 2015, from:
http://alevelnotes.com/magnification/106
- Resteck, G. (2015). Squishy Science: Extract DNA from Smashed Strawberries.
[online] Scientificamerican.com. From: http://www.scientificamerican.com/article/squishyscience-extract-dna-from-smashed-strawberries/ Accessed 12th May 2015
- Seqcore.brcf.med.umich.edu, (2015). DNA Denaturation, Annealing and
Replication. [online] Available at:
http://seqcore.brcf.med.umich.edu/doc/educ/dnapr/pg2.html
Accessed 13 May 2015