Materials

Part 1
-3 fishes with their water
-3 beakers (2x500mL and 1x300mL)
-20 wooden disposable chopsticks
-20 bamboo disposable chopsticks
-pH-meter
-Distilled water
Part 2
-3 final solutions from the Part 1
-6 test tubes
-Sulfuric acid
-Barium chloride
Part 3
-4 water bottles (500mL)
-20 wooden disposable chopsticks
-20 bamboo disposable chopsticks
-4 beakers (500mL)
-Aluminum Foil
-Manganese oxide
Procedure
Part 1
1. Pour the water which the fishes were living into the three beakers.
2. Label the 500mL beakers as A and B.
3. Label the 300mL beaker as C.
4. Measure the pHs of each water in three beakers and distilled water.
Record the values into the proper table.
5. Carefully transfer three fishes to each beaker.
6. Place 20 wooden disposable chopsticks into the beaker A. Make sure not
the stress the fish.

7. Place 20 bamboo disposable chopsticks into the beaker B. Make sure not
to stress the fish.
8. Continue measuring the pHs every 1 hour until the fishes in the beakers
A and B are dead. Record the pHs and the time fish died.
Part 2
1. Label the test tubes as A1, A2, B1, B2, C1, and C2.
2. Pour the 3 final solutions from Part 1 to corresponding test tubes.
3. Pour sulfuric acid to the test tube A1. Smell the odor and record.
4. Pour sulfuric acid to the test tube B1. Smell the odor and record.
5. Pour sulfuric acid to the test tube C1. Smell the odor and record.
6. Pour Barium chloride to the test tube A2. Record the observation.
7. Pour Barium chloride to the test tube B2. Record the observation.
8. Pour Barium chloride to the test tube C2. Record the observation.
Part 3
1. Cut disposable chopsticks into small pieces.
2. Cover the 500mL bottles with aluminum foil so that light would not
enter.
3. Label the bottles as A1, A2, B1, and B2.
4. Put wooden disposable chopstick pieces as many as 10 whole disposable
chopsticks into the bottle A1 and fill it with water.
5. Put bamboo disposable chopstick pieces as many as 10 whole disposable
chopsticks into the bottle B1 and fill it with water.
6. Put wooden disposable chopstick pieces as many as 10 whole disposable
chopsticks into the bottle A2 and fill it with vinegar.

7. Put bamboo disposable chopstick pieces as many as 10 whole disposable

chopsticks into the bottle B2 and fill it with vinegar.
8. Leave 4 bottles for 3 days.
9. Pour the solution from A1 to 500mL beaker and quickly add manganese
oxide to it. Record the observation.
10.Repeat #9 for B1, A2, and B2. Record the observations.
*The difference between the size of the beakers was done in order to equalize
the space available for the fishes after the chopsticks were put into.