PHILIPPINE ASSOCIATION OF SCHOOLS OF MEDICAL TECHNOLOGY AND PUBLIC HEALTH

Prototype Course Syllabus

Course Title: Hematology 1 (Clinical Hematology)

Course Description:

This course deals with the study of blood as a tissue and the pathophysiology of the cellular elements of the blood. Emphasis is on laboratory diagnostic procedures.

Course Credit: 3 units (2 units lecture, 1 unit laboratory)

Contact Hours: 2 hours lecture, 3 hours laboratory per week (36 hours lecture and 54 hours laboratory per semester)

Prerequisites: Biochemistry, Human Anatomy and Physiology

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Placement: Third Year, 2 Semester

Terminal Competencies: At the end of this course, the student is able to:
1. explain the facts and principles of hematological determinations;
2. identify microscopically the normal and abnormal cells,
3. assume responsibility in the collection and handling of blood specimens
4. correlate the importance of hematological tests in the diagnosis and prognosis of diseases,
5. apply concepts of quality assurance in Hematology
6. perform hematological tests with precision, accuracy and reliability
7. develop the necessary skills such as technical, clinical, mathematical and judgment skills
8. manifest responsibility, cooperation, self reliance, honesty, critical thinking, empathy and value for life.

References:
1. Anderson S. and K. Poulsen. Anderson’s Atlas of Hematology. USA: Lippincott Williams and Wilkins, 2003.
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2. Brown, Barbara. Hematology: Principles and Procedures 6 ed. Philadelphia: Lea and Febiger, 1993.
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3. Carr, Jaqueline and Bernadette Rodak. Clinical Hematology Atlas 3 ed. Philadelphia: W.B. Saunders, 2008.
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4. Garza, Diana and Kathleen Becan-McBride. Phlebotomy Handbook: Blood Collection Essentials 7 ed. USA: Prentice Hall, 2004.
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5. Greer J., J. Foerster and J. Lukens. Wintrobe’s Clinical Hematology 11 ed. USA: Lippincott Williams and Wilkins, 2003.
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6. Harmening D. Clinical Hematology and Fundamentals of Hemostasis. 5 ed. USA: F.A. Davis Co., 2008.
7. Hoeltke, Lynn. Phlebotomy: Principles and Procedures. USA: Delmar Learning, 2006.

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 th
8. Hoffbrand V., P. Moss and J. Pettit. Essential Haematology 5 ed. USA: Wiley-Blackwell, 2006.
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9. Hoffman R. et.al. Hematology: Basic Principles and Practice 4 ed. USA: Churchill Livingstone, 2004.
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10. McClatchey, Kenneth. Clinical Laboratory Medicine 2 ed. USA: Lippincott Williams and Wilkins, 2002.
11. Mckenzie, Shirley, Clinical Laboratory Hematology. USA: Prentice Hall, 2006.
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12. Mcpherson, Richard A. and Matthew R. Pincus. Henry’s Clinical Diagnosis and Management by Laboratory Methods 21 ed. Philadelphia: Elsevier Inc., 2007.
13. Reddi, V., M. Marques and G. Fristma. Quick Guide to Hematology Testing. USA: AACC Press, 2007.
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14. Rodak B., G. Fritsma and K. Doiq. Hematology: Clinical Principles and Applications 3 ed. Philadelphia: W.B. Saunders, 2007.
15. Schmaier A. and L. Petruzelli. Hematology for the Medical Student. USA: Lippincott Williams and Wilkins, 2003.
nd
16. Scott M., A. Gronowski and C. Eby. Tietz’s Applied Laboratory Medicine 2 ed. USA: Wiley-Liss, 2007.
17. Steininger, Cheryl et al. Clinical Hematology: Principles, Procedures and Correlations. Philadelphia: J.B. Lippincot, 1992.
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18. Theml, H. Color Atlas of Hematology: Practical, Microscopic and Clinical Diagnosis 2 ed. USA: Thieme, 2004.
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19. Turgeon, Mary Louise. Clinical Hematology: Theory and Procedures 4 ed. USA: Lippincott Williams and Wilkins, 2004.

Electronic References:
1. http://wps.prenhall.com/chet_mckenzie_clinicallab.
2. http://www.bloodline.net/
3. http://www.emedicine.com/med/topic132.htm
4. http://www.hematologyatlas.com/
5. http://www.wadsworth.org/chemheme/heme/cytoheme/cyto35mm.htm

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 Learning Objectives / T.A. Teaching Strategies Skills Values Evaluation/Assessment
Intermediate Competencies Content
Lecture Laboratory
At the end of each unit, the student is I. Introduction to Clinical Hematology Clinical Case Actual Lab. Technical Skills Appreciation of Unit Test Exam
able to: Lecture 2 Study Performance Critical thinking the importance Practical Exam
1. define terms related to Clinical 1. Definition of Terms hrs Report Back - Skin skills of quality Performance Checklist
Hematology 2. Basic Hematological Methods of Examination Session Puncture assurance Return
2. explain the different hematological 3. Quality Assurance Program Lecture/ - Venipuncture program. Demonstration
methods of examination 4. Collection of Blood Specimens Discussion - Return Demo Honesty Empathy Class
3. Explain the quality assurance 4.1 Venipuncture Power point and value for life Participation in all
program for clinical hematology 4.2 Skin puncture Presentation Laboratory Activities
4. Perform properly and accurately CD viewing/
the methods of blood collection. II. Morphology of Blood Cells Discussion
5. define terms related to morphology Lecture: 8 Report Back
of blood cells 1. Definition of Terms hrs Session/
6. describe the cells of the different 2. Hematopoietic System Post
series 2.1. Erythropoiesis Conference
7. differentiate mature from immature 2.2. Granulopoiesis
cells 2.3. Lymphopoiesis
8. identify the cells microscopically 2.4. Monopoiesis
9. explain the morphological 2.5. Megakaryopoiesis
characteristics of the RBC and 3. Cell Abnormalities
WBC anomalies 3.1. Red Blood Cell Anomalies
10. identify properly the different 3.2. White Blood Cell Anomalies
variants microscopically 3.3. Platelet Anomalies

Laboratory: 12
1. Collection of Blood Specimens hrs
1.1. Venipuncture
1.2. Skin Puncture
2. Microscopic Examination of prepared blood
smears
3. Laboratory Safety
4. Laboratory Waste Management
At the end of each unit, the student is III. Routine Blood Examination Clinical Case Actual Lab. Technical skills Cooperation Unit Test Exam
able to: Lecture: 8 Study Performance Judgment skills Self-reliance Practical Exam
1. recall hemoglobin synthesis 1. Review of Hemoglobin Synthesis hrs Report Back - Hemoglobin Honesty Performance Checklist
1.1. perform accurately different 1.1. Structural formulae Session electrophoresis Return
methods of hemoglobin 1.2. Molecular formulae Lecture/ - Hemoglobin Demonstration
determination 2. Hemoglobinopathies Discussion Determination Class
2. differentiate the methods of 2.1. Hemoglobin Electrophoresis Power point - ESR Participation in all
hematocrit and erythrocyte 3. Hemoglobinometry Presentation Determination Laboratory Activities
sedimentation rate (ESR) 3.1. Methods of Hemoglobin Determination CD viewing/ - Problem
determination 4. Hematocrit and Erythrocyte Sedimentation Discussion Solving
2.1. perform properly the (ESR) Rate Determinations Report Back - Return Demo
different methods of 4.1. Methods of Hematocrit and ESR Session/

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 hematocrit & ESR Determinations Post
determinations 4.1.1. Micromethod Conference
2.2. Relate results of hematocrit 4.1.2. Macromethod
and ESR to human diseases 4.2. Physiologic and Pathologic Variations
of Hematocrit and ESR

Laboratory: 15
1. Hemoglobin Determination hrs
2. Hematocrit Determination
3. ESR Determination
4. Laboratory Safety
5. Laboratory Waste Management
At the end of each unit, the student is IV. Principles in Blood Counting Clinical Case Actual Lab. Demonstrate Accuracy Unit Test Exam
able to: Lecture 9 Study Performance critical thinking Reliability Practical Exam
1. explain the principles involved in 1. Enumeration of Various Blood Cells hrs Report Back - Erythrocyte skills in relating Performance Checklist
the methods of cell counting 1.1. Erythrocyte Count Session Count results to Return
2. develop the skill in performing 1.2. Leukocyte Count Lecture/ - Leukocyte diseases Demonstration
cell count and the proper use of 1.3. Thrombocyte Count Discussion Count Class
equipment and reagents 1.4. Eosinophil Count Power point - Thrombocyte Participation in all
3. Interpret the results obtained to 1.5. Basophil Count Presentation Count Laboratory Activities
diseases affecting man 1.6. Reticulocyte Count CD viewing/ - Eosinophil
4. explain the sources of errors in 1.7. Siderocyte Count Discussion Count
cell counting Report Back - Basophil
Laboratory: 15 Session/ Count
1. Performance of Blood Counting hrs Post - Reticulocyte
1.1. Automated Methods Conference Count
1.2. Conventional Methods - Siderocyte
1.3. Unopette System Count
2. Laboratory Safety - Problem
3. Laboratory Waste Management Solving
- Return Demo
At the end of each unit, the student is V. Blood Indices and Blood Constant Clinical Case Actual Lab. Technical skills Accuracy Unit Test Exam
able to: Lecture: 9 Study Performance Mathematical Reliability Practical Exam
1. define terms related to blood 1. Definition, Formulas and Reference Values hrs Report Back - Computation skills Performance Checklist
indices and blood constant 1.1. MCH Session of Blood Judgment skills Return
1.1. apply correctly the formula 1.2. MCV Lecture/ Indices Demonstration
in solving problem related to 1.3. MCHC Discussion - Smear Class
blood indices and constants 1.4. MCAT Power point Preparation Participation in all
1.2. relate obtained results to 1.5. MCD Presentation - Routine Laboratory Activities
diseases affecting man 1.6. RDW CD viewing/ staining
2. prepare properly blood smears 2. Preparation of Blood Smears Discussion - Cytochemical
3. explain the principle of differential 2.1. Thin Smears Report Back staining
count 2.1.1. Methods e.g. Wedge, Cover Session/ - Problem
3.1. differentiate the shifting Glass, Spinner’s Methods, etc. Post solving
processes 2.2. Thick Smears Conference - Return Demo
3.2. perform accurately the 2.2.1. Methods
differential count 3. Differential Leukocyte Count

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4. explain the use of routine stains 3.1. Principles
5. explain the principles of the 3.1.1. Shifting Processes
different cytochemistry 3.2. Methods
procedures 4. Routine Stains
5. Cytochemistry

Laboratory: 12
1. Laboratory seatwork on blood indices hrs
2. Preparation of blood smears
3. Staining of blood smears
4. Differential count
5. Laboratory Safety
6. Laboratory Waste Management

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 Technical Working Group for Prototype Course Syllabi Development
(2007-2008)

Zennie Aceron Frederick Llanera Anacleta Valdez

Petrona Benitez Carina Magbojos Rowen Yolo
Sergia Cacatian Gregorio Martin
Zenaida Cajucom Fe Martinez and other PASMETH members
Edwin Cancino Josephine Milan not cited in this page who in
Jacinta Cruz Ferdinand Mortel one way or another has
De Carlos Leon Magdalena Natividad contributed greatly to the
success of this endeavor…
Oliver Shane Dumaoal Rodolfo Rabor
Bernard Ebuen Ma. Teresa Rodriguez
Nini Lim Celia Roslin

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