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Abstract

Name: Geoffrey D. Panjeton

Home Address: 116 Ibisca Terrace


Royal Palm Beach, FL 33411
High School: Suncoast Community High School
Research Advisor: Dr. Lokenga Badinga and Cristina Caldari-Torres
Department of Animal Sciences
University of Florida, Gainesville

Modulation of Acute Phase Protein Production by


Omega-3 and Omega-6 Fatty Acids in Cultured Human Hepatocytes

The altered production of acute phase proteins by the liver is one of the main characteristics of
the systemic inflammatory response in human disease. The objective of this study was to investigate
the effects of omega-3 and omega-6 fatty acids on acute phase proteins production by HepG2 cells.
Confluent HepG2 cells were treated with linolenic acid , alpha linoleic acid, or eicosapentaenoic acid in
the presence or absence of tumor necrosis factor-alpha or interleukin-6 or a combination of the two
cytokines. The stimulation of HepG2 cells resulted in negligible production of haptoglobin or C-
reactive proteins, suggesting that an immortalized hepatoma cell line may not be an ideal model to
examine the effects of cytokines on immune responses in the human liver.

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