A.

Young Enzyme Concentration Lab Abstract

The purpose of this experiment was to see if enzyme concentration affects the rate of
the reaction between catalase and H2O2 (hydrogen peroxide). Within the experiment, different
levels of enzyme concentration were tested. Catalase, which was the enzyme in the
experiment, was water down to create different concentration levels in the experiment. They
were then added to the H2O2 and the pressure that the product created was measured by a
pressure probe. In the 100 percent concentration trial, the initial rate was 0 k/Pa, at 60 seconds
the rate was 0.28 k/Pa, and the final rate was 0.01 k/Pa. In this trial the rate decreased steadily.
In the 80 percent concentration trial, the initial rate was 0 k/Pa, at 60 seconds the rate was 0.14
k/Pa, and the final rate was 0.04 k/Pa. In this trial, the rate decreased at about the same time as
the 100 percent concentration trial. In the 60 percent concentration trial, the initial rate was 0
k/Pa, at 60 seconds the rate was 0.21 k/Pa, and the final rate was 0.05 k/Pa. In this trial, the
rate decreased more drastically. In the 40 percent concentration trail, the initial rate was 0
k/Pa, at 60 seconds the rate was 0.07 k/Pa, and the final rate was 0.04 k/Pa. In this trial, the
rate almost decreased instantly. Although the rate decreased, the amount of products
continuously increased. The graph below shows the pressure that was built up as time elapsed.
Naturally, the higher the concentration, the more products, or pressure, that was produced.
Therefore, the hypothesis for the experiment was supported by the results.
A. Young Enzyme Concentration Lab Abstract