NANO-IMAGING

NEAR-FIELD SCANNING OPTICLAL MICROSCOPY (NSOM)

CONFOCAL MICROSCOPY FOR BIOIMAGING
T. Vo-Dinh* and H.K. Yuan
Fitzpatrick Institute for Photonics
Duke University
Durham, North Carolina, USA
Confocal microscopy and NSOM have been developed and applied in our laboratory to monitor the
localization and effect of cellular components. Fundamental studies have demonstrated that
multidrug resistance (MDR) transport protein, MDR1 and MRP1 are responsible for the decreased
intracellular accumulation of the acthracycline doxorubicin in normal and tumor cells. Therefore
understanding the intracellular localization these proteins may offer new strategies for
chemotherapeutic agents in a clinical setting . These technologies will have practical applications
in environmental sensing, pathogen diagnostics and medical therapy.

NSOM Imaging
Detection and Localization of MDR Proteins in Single Cell
(AT3B-1 cell incubated with fluorescent-labeled Line1

anti-Pgp antibodies) Point1:

| X | (µm)
0.610
Z(V)
0.148430
Point2: 0.560 0.145403
Diff: -0.049 -0.003027
Length: 0.049 µm

C
0.2 V

0.15
Z Data

0.1

0.05
B

0
0 0.26 0.52 0.78 1.04 1.3 µm
Distance

A D E

NSOM image of a CHO cell incubated with

doxorubicin (DOX) and Pgp-inhibitor
verapamil (VP). Left: Topography image
of the cell. Middle: NSOM fluorescence
image showing the localization of DOX
within the cell nucleus. Right: A merged
image

*Technical Contact : Prof. Tuan Vo-Dinh (tuan.vodinh@duke.edu)