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AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY 119:231239 (2002)

Age Estimation From the Auricular Surface of the Ilium: A Revised Method
J.L. Buckberry* and A.T. Chamberlain
Department of Archaeology and Prehistory, University of Shefeld, Shefeld S1 4ET, UK KEY WORDS auricular surface; ilium; skeletal age at death; Spitalelds; forensic anthropology; palaeodemography ABSTRACT A revised method for estimating adult age at death using the auricular surface of the ilium has been developed. It is based on the existing auricular surface aging method of Lovejoy et al. ([1985] Am. J. Phys. Anthropol. 68:1528), but the revised technique is easier to apply, and has low levels of inter- and intraobserver error. The new method records age-related stages for different features of the auricular surface, which are then combined to provide a composite score from which an estimate of age at death is obtained. Blind tests of the method were carried out on a known-age skeletal collection from Christ Church, Spitalelds, London. These tests showed that the dispersion of age at death for a given morphological stage was large, particularly after the rst decade of adult life. Statistical analysis showed that the age-related changes in auricular surface are not signicantly different for males and females. The scores from the revised method have a slightly higher correlation with age than do the Suchey-Brooks pubic symphysis stages. Considering the higher survival rates of the auricular surface compared with the pubic symphysis, this method promises to be useful for biological anthropology and forensic science. Am J Phys Anthropol 119:231239, 2002.

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The estimation of age at death of adult skeletal material is one of the more difcult tasks undertaken by physical anthropologists. Mays (1998, p. 50) stated that at present the lack of a wholly satisfactory technique for estimating age at death in adult skeletons from archaeological sites is one of the most thorny problems facing human osteoarchaeology. The problem arises from the fact that rates of skeletal remodeling and degeneration, from which most methods of adult age estimation are derived, can be highly variable between different individuals and populations. The life history of an individual will be an important determinant of the rate of skeletal aging. Factors such as endocrine status, diet, disease, physical activity, and cultural differences will contribute to the range of variation between males and females, old and young, and rich and poor within a site, and also between populations from different sites (Brothwell, 1981; Ubelaker, 1989; Schwartz, 1995; Hillson, 1996; Aykroyd et al., 1999). Even with a similar biological background and environmental conditions, two individuals are likely to age at different rates. Pubic symphysis age estimation was rst applied to adult males (Todd, 1920), and was subsequently redened and tested, especially with regard to creating separate systems for females (Brooks, 1955; Nemeskeri et al., 1960; Gilbert and McKern, 1973; Suchey, 1979; Katz and Suchey, 1986; Brooks and Suchey, 1990). These methods were found to generate age distributions for different populations that

mirrored the age structure of the reference series from which the methods were developed (BocquetAppel and Masset, 1982), and did not allow for the variation normal to a skeletal population (Suchey et al., 1986). When the Suchey-Brooks method (Brooks and Suchey, 1990) was developed, this variation was made explicit by the documentation of the dispersion of age at death for each morphological stage of the pubic symphysis. The auricular surface method of age estimation has not been subject to the same levels of scrutiny as pubic symphysis aging, and it is usually applied in the form originally developed by Lovejoy et al. (1985). Murray and Murray (1991) tested the auricular surface method of Lovejoy et al. (1985) on a sample of skeletons in the Terry Collection, to see how reliable the method was for use in forensic cases. They found that the method was unbiased regarding race and sex (Murray and Murray, 1991;
Grant sponsor: Universities of Shefeld, York, and Leeds. *Correspondence to: Miss Jo Buckberry, Research School of Archaeology and Archaeological Science, University of Shefeld, West Court, 2 Mappin St., Shefeld S1 4DT, UK. E-mail: J.Buckberry@Shefeld.ac.uk Received 23 July 2001; accepted 17 April 2002. DOI 10.1002/ajpa.10130 Published online in Wiley InterScience (www.interscience.wiley. com).

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p. 1166). However, they also found that the method consistently underestimated the age of older individuals, and overestimated the age of younger individuals in their sample. They felt that this might have been attributable to differences in age structure between the Todd Collection, on which the method was developed, and the Terry Collection (Murray and Murray, 1991). Murray and Murray (1991) concluded that the method was too unreliable to be used as a single aging technique for forensic science purposes. However, they conceded that the method would be useful when used in conjunction with other methods of age estimation. The auricular surface method was also tested on an archaeological sample of known age at death from Belleville, Ontario (Saunders et al., 1992). The system was found to underestimate age at death especially for the older portion of the sample (over 45 years), although it predicted the ages of younger adults reliably (Saunders et al., 1992). The authors found that estimates of age at death for many individuals did not fall into the correct modal stages, indicating that the method of Lovejoy et al. (1985) may not allow adequately for individual variation in skeletal aging. It was also noted that intraobserver error was high (19.3%) (Saunders et al., 1992), and this may be due to the difculty found in applying the method, especially when classifying ambiguous specimens . . . that cannot with certainty be assigned to one age category (Saunders et al., 1992, p. 99). Bedford et al. (1993) also tested the auricular surface method on a sample of known-age skeletons from the Grant Collection at the University of Toronto. As with other tests of the method, there was a tendency to overestimate ages of younger adults and to underestimate ages in categories above 60 years old. AURICULAR SURFACE METHOD OF AGE ESTIMATION OF LOVEJOY ET AL. (1985) The age-related changes seen on the auricular surface of the ilium were rst described by Sashin (1930). He interpreted the changes seen in the articular cartilage of the joint as osteoarthritic, and found that they were progressive, and increase in extent and intensity with the age of the individual (Sashin, 1930, p. 909). However, the regularity of these changes was not discussed. The age-related changes of the auricular surface were not exploited as a method of estimating age at death until Lovejoy et al. (1985) developed their technique. Lovejoy et al. (1985) described eight modal age stages (typically encompassing 5 years), into which each auricular surface could be placed using primary aging features. An age would then be estimated from within this modal stage, using secondary indicators of age (apical activity and retroauricular activity). Auricular surface age estimation increased the range of methods that were available, and the au-

ricular surface area of the ilium is more durable than the fragile pubic symphysis, with higher levels of survival and recovery, particularly in archaeological populations (Lovejoy et al., 1985; Waldron, 1987). However, the separate features of the auricular surface described by Lovejoy et al. (1985), such as porosity, surface texture, and marginal changes, appear to develop independently of each other. The age of onset for each stage of different features of the auricular surface appears to vary, and as a consequence the 5-year age categories of Lovejoy et al. (1985) tend to overlap. Early-appearing features still present on the auricular surfaces of older individuals were described by Lovejoy et al. (1985) as residual. The fact that this variation can occur within a single auricular surface indicates that the method of Lovejoy et al. (1985) oversimplies the changes seen, and that the 5-year intervals in their scheme of age estimation may be optimistically narrow. This problem contributes to the difculty found with applying the method, as it leads to uncertainty, and in some cases confusion, in assigning individual auricular surfaces to a particular age stage. A REVISED AURICULAR SURFACE METHOD OF AGE ESTIMATION In view of these problems, we believe that the auricular surface age estimation technique would benet from the same level of reanalysis that has been undertaken for pubic symphyseal age estimation. A quantitative scoring system, which examines each different feature of the auricular surface independently, should not only be easier to apply but will also accommodate the overlap often seen between different stages. Our revised system has been developed from that of Lovejoy et al. (1985), and utilizes their categories of age-related change seen on the auricular surface. The features used were transverse organization, surface texture, microporosity, macroporosity, and changes in morphology of the apex and retroauricular area. Each of the features seen on the auricular surface was recorded independently and assigned a series of numerical scores corresponding to successive stages of degrees of expression. Although one feature might be obscured by another, the use of standardized criteria (dened below) allowed them to be assessed objectively. In preliminary analyses, the retroauricular area was found to be a poor estimator of age, and this led to its exclusion from the revised method. The surface is described using the same terminology employed by Lovejoy et al. (1985). Figure 1 shows the locations used in describing the auricular surface. Table 1 gives the descriptions of each location, as dened in Buikstra and Ubelaker (1994). Each of the features of the auricular surface is described in detail, with a summary description of each stage observed given in tabular form.

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TABLE 3. Scoring system for surface texture Score 1 2 3 4 5 Description

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90% or more of surface is nely granular 5089% of surface is nely granular; replacement of nely granular bone by coarsely granular bone in some areas; no dense bone is present 50% or more of surface is coarsely granular, but no dense bone is present Dense bone is present, but occupies less than 50% of surface; this may be just one small nodule of dense bone in very early stages 50% or more of surface is occupied by dense bone TABLE 4. Scoring system for microporosity

Score 1 2 3

Description No microporosity is present Microporosity is present on one demiface only Microporosity is present on both demifaces

Fig. 1. Regions of the ilium used for auricular surface aging (redrawn after Lovejoy et al., 1985). TABLE 1. Definitions used to describe auricular surface (after Buikstra and Ubelaker, 1994) Denition Apex Superior demiface Inferior demiface Retroauricular area Description of locations Portion of auricular surface that articulates with posterior aspect of arcuate line Portion of auricular area above apex Portion of auricular area below apex Region between auricular surface and posterior inferior iliac spine

TABLE 2. Scoring system for transverse organization Score 1 2 3 4 5 Description 90% or more of surface is transversely organized 5089% of surface is transversely organized 2549% of surface is transversely organized Transverse organization is present on less than 25% of surface No transverse organization is present

auricular surface is nely grained in early life, and becoming more coarsely granular and densied in older individuals. As with transverse organization, this feature is scored in terms of what proportion of the surface is covered by a particular type of texture. Finely granular bone is dened as having grains predominantly less than 0.5 mm in diameter, and coarsely granular bone consists of grains predominantly over 0.5 mm in diameter. Dense bone refers to surface appearance, rather than the amount of bone present. It is dened as nodules or areas of bone which are compact and smooth, with no surface granularity. The scoring system for surface texture is given in Table 3. Microporosity In the revised system, microporosity was dened as porosity of the surface (or perforations of subchondral bone), with the pores having a diameter of less than 1 mm. It is scored according to the presence of microporosity on one or both of the two demifaces of the auricular surface. Microporosity may be localized or spread across large areas. Lovejoy et al. (1985) regarded microporosity as a secondary aging feature. The scoring system for microporosity is given in Table 4. Macroporosity Macroporosity also perforates the surface of the bone. We dene macroporosity as holes greater than 1 mm in diameter. Like microporosity, it may be localized or spread across large areas. Lovejoy et al. (1985) also considered macroporosity a secondary aging feature. Macroporosity is scored according to its presence on one or both of the two demifaces of the auricular surface, in the same manner as for microporosity. The scoring system for macroporosity is given in Table 5. Macroporosity should not be confused with cortical defects, which may be present at any age. Cortical defects are areas where the cortex of the bone is

Transverse organization Transverse organization refers to the horizontally orientated billows and striae that run from the medial to the lateral margins of the auricular surface (Lovejoy et al., 1985). It was difcult to distinguish between billows and striae and to be consistent in the recording of these features. Consequently, this trait is scored in terms of what proportion of the auricular surface is transversely organized. The proportion is estimated by eye, rather than measured, and is assigned to 1 of 5 easily distinguishable stages dened in terms of the percentage of surface covered. The scoring system for transverse organization is given in Table 2. Surface texture This feature corresponds to that described as grain by Lovejoy et al. (1985). The texture of the

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TABLE 5. Scoring system for macroporosity TABLE 8. Multiple regression of age against features Feature (constant) Transverse organization Surface texture Microporosity Macroporosity Apical changes Unstandardized coefcients 6.500 4.316 7.105 5.572 4.669 3.282 t 1.156 2.527 4.115 3.680 2.720 2.675 P 0.249 0.012 0.001 0.001 0.007 0.008

Score 1 2 3

Description No macroporosity is present Macroporosity is present on one demiface only Macroporosity is present on both demifaces TABLE 6. Scoring system for apical changes

Score 1 2 3

Description Apex is sharp and distinct; auricular surface may be slightly raised relative to adjacent bone surface Some lipping is present at apex, but shape of articular margin is still distinct and smooth (shape of outline of surface at apex is a continuous arc) Irregularity occurs in contours of articular surface; shape of apex is no longer a smooth arc

tographed were specically chosen to illustrate the typical appearance seen at a particular age. Blind test on a known-age sample The new scoring system was then tested by one of the authors (J.L.B.) on a known-age skeletal population (n 180) from Christ Church, Spitalelds, London (held at the Natural History Museum, London; Molleson and Cox, 1993), to assess how the features related to chronological age. Each auricular surface in the collection was recorded twice, with an interval of 2 weeks between these analyses. Any discrepancies between scores were noted, and if discordant, the surface was studied a third time. The age and sex of the individual was not recorded on the boxes, and care was taken that the observer did not know this information prior to recording. While the sex of strongly dimorphic individuals would be apparent to the observer, it was not felt that this would cause bias, as the recording criteria were rigorous, and applied solely to the auricular surface and not to the surrounding bone. The observer also avoided examining other parts of the skeleton, which may have given subjective clues to age at death. Color photographs were taken of every auricular surface. Once the study was completed, the photographs were seriated for each feature, to check the consistency of the recording. The scores were then entered into a computer database along with the documented age and sex of each individual. RESULTS Although Kendalls coefcient of concordance indicates a reasonable measurement of agreement among the ve features (Wa 0.691, P 0.0005), each feature supplies independent information about age. This is seen in the results of a stepwise multiple regression, which indicates that all ve features contribute to the prediction of age (Table 8), and in the partial correlations between features after controlling for the effect of age (Table 9). The partial correlations among the features are low and mostly nonsignicant, conrming that the features provide independent sources of information about age. The combination of transverse organization, surface texture, microporosity, macroporosity, and apical changes was found to give the highest correlation with age (rs 0.609; P 0.01). This composite score, calculated as the sum of the scores of the

TABLE 7. Spearman correlations between age and feature for auricular surface photographs of Bedford et al. (1989) Feature Transverse organization Surface texture Microporosity Macroporosity Apical changes rs 0.942 0.930 0.654 0.883 0.888 P 0.01 0.01 0.01 0.01 0.01

not complete. They are usually smooth-edged and do not cover a signicant area of the auricular surface. Macroporosity may also be confused with areas of postmortem damage, which are usually sharp-edged and irregular, often have paler colored bone around the edges, and may expose underlying trabecular bone that is always unremodelled. Care should be taken in recording this feature, to ensure that no confusion with postmortem damage has arisen. Apical changes The apex of the auricular surface can develop small osteophytic growths, or lipping, which when more severe can alter the contour of the surface. This change was regarded as a secondary aging feature by Lovejoy et al. (1985), and was described in terms of activity. The scoring system for apical changes is given in Table 6. Preliminary testing The new scoring system was initially applied to archaeological skeletal material of unknown age at death from the Anglo-Norman site of Blackgate, Newcastle-upon-Tyne (n 56; held at the Department of Archaeology and Prehistory, University of Shefeld), to test for ease of use and to check for reproducibility of results. The scoring system was then applied to photographs of known-age auricular surfaces distributed by Bedford et al. (1989) that accompany the system of Lovejoy et al. (1985), to see how well the individual features of the auricular surface correlated with age (Table 7). Each feature was found to have a high correlation with age, consistent with the fact that the auricular surfaces pho-

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TABLE 9. Partial correlation coefficients between features controlling for effects of age1 TO TO ST MI MA AP 0.001 0.070 0.004 0.590 ST 0.381 0.065 0.001 0.023 MI 0.136 0.138 0.164 0.612 MA 0.217 0.316 0.104 0.429 AP 0.040 0.169 0.038 0.059

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TABLE 10. Independent two-tailed t-tests between males and females for each composite score Composite score 56 78 910 1112 1314 1516 1719 Age stage I II III IV V VI VII t df P

1 Coefcients above diagonal, p-values below, sample size n 180. TO, transverse organization; ST, surface texture; MI, microporosity; MA, macroporosity; AP, apical changes.

Sample size too small 0.331 4 0.757 1.825 20 0.083 0.373 30 0.711 0.005 62 0.996 0.142 39 0.888 1.125 10 0.287

Fig. 2. Plot of composite auricular surface scores against age for Spitalelds sample. Spearmans correlation coefcient of score against age is given for males and females.

with age (Table 11). Although the r values are relatively low, all were signicant at the 99% condence level. The scores were combined to produce a composite score, which increased the correlation with age (males, rs 0.624; females, rs 0.626; P 0.01). Several composite scores were found to have similar ranges, distributions, and mean ages. These scores were grouped together to produce seven auricular surface stages for the purposes of age estimation. The age ranges, means, and median ages for these stages are given in Table 12. It should be noted that the mean age for auricular surface stage is affected by the age structure of the reference sample, which in this case is biased toward older ages (Bocquet-Appel and Masset, 1982; Jackes, 1992). To compensate for this, a Bayesian analysis was undertaken, using uniform priors following the method of Chamberlain (2000) to provide posterior probabilities of age, given the auricular surface stage (Table 13). Reliability testing The method was tested for intraobserver error by one of the authors (J.L.B.). Six well-preserved auricular surfaces were recorded twice, at an interval of 2 weeks. A paired t-test showed no signicant variation (P 0.05) between the composite scores given on each occasion (t 1.348; df 5; P 0.235). A different group of six auricular surfaces was tested for interobserver error by graduate students in osteology at the University of Shefeld and members of the British Association of Biological Anthropology and Osteoarchaeology. Cohens weighted kappa measure of agreement for ordinal data indicated a low level of interobserver error ( 0.66) (Agresti, 1988). The Spitalelds sample was also recorded using the Suchey-Brooks pubic symphysis method, which was applied using the casts for males and females (Suchey et al., 1988; Brooks and Suchey, 1990). The pubic symphysis was only present for 58.9% of the sample. The rank order correlation between the six Suchey-Brooks stages and age is rs 0.58; P 0.01. This compared favorably with the correlation between the new seven-stage auricular surface aging method and age (rs 0.63; P 0.01). This shows that although the Suchey-Brooks system is thought to be the most reliable skeletal indicator of age (Mays, 1998), the new auricular surface aging

separate features, was used in all subsequent statistical tests. A paired t-test was calculated for all individuals with both left and right auricular surfaces present. The results showed no signicant difference for the composite score between the left and right sides (t 0.6; df 157; P 0.550). Subsequently, the left side was used for all analyses when both sides were available, and the side available was used for all other individuals. Previous analyses had revealed no signicant difference in age-related changes of the auricular surface between males and females (Lovejoy et al., 1985; Murray and Murray, 1991). The ages were plotted against composite scores for males and females as a scatter plot, with a regression line for both data sets (Fig. 2). The regression lines indicate that there is little difference between sexes (males, rs 0.624, P 0.01; females, rs 0.626, P 0.01). A standard (two-sample, two-tailed) t-test was also carried out to test for signicant differences between males and females for each of the score categories. There were no signicant differences between ages for males and females (P 0.05; see Table 10). A scatter plot of score against age for each feature, shown in Figure 3, reveals wide variation in the age of appearance of the features. A Spearmans correlation coefcient was calculated for each feature

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Fig. 3. Scatter plots of scores against age at death in Spitalelds sample for each of ve features: transverse organization, surface texture, microporosity, macroporosity, and apical changes. TABLE 11. Spearmans correlation with age for each feature and for composite score Feature Transverse organization Surface texture Apical changes Microporosity Macroporosity Composite score rs 0.433 0.489 0.319 0.408 0.533 0.609 P 0.005 0.005 0.005 0.005 0.005 0.005

method has a higher correlation with age for the Spitalelds sample. DISCUSSION AND CONCLUSIONS Individual human skeletons show age-related changes which progress at different rates. It would appear that none of the current methods of skeletal age estimation are able to provide age estimates that are both precise (closeness of repeated measurements) and accurate (closeness to true value) (Sokal and Rohlf, 1995, p. 50), and that such precise levels of age estimation are unlikely to be developed in the foreseeable future. While precise age estimates are desirable and will always be sought, it is not feasible to attempt to provide them if they are not a true reection of the underlying biology. None of the present age-estimation methods are able to provide estimates within narrow age ranges, so consequently age estimates need to be stated either as broad ranges or as probability density functions (e.g., Jackes, 1992; Chamberlain, 2000). Multiple regression shows that the features of the auricular surface age independently of each other, indicating that each feature has to be interpreted independently, rather than being grouped together

with other features into 5-year modal age stages. Our revised auricular surface method of age estimation allows for a more realistic interpretation of the changes. Although the age estimates produced by our method are wider, this revised method is easier to apply and may be more reliable than that of Lovejoy et al. (1985). The sample size used to develop the method was small (n 180), with younger individuals underrepresented due to the age structure of the Spitalelds Collection. The method outlined here needs to be tested and redened, using large, multiracial, and known-age modern and, if possible, archaeological populations. This will lead to the redenition of age ranges and standard deviations. Although Murray and Murray (1991) showed that the method of Lovejoy et al. (1985) was equally applicable for different populations, this has not yet been demonstrated for our revised method. Such a study is needed to determine if the method is universally applicable across human populations, and will have important implications for the applicability of the method to archaeological samples. It has also been shown that there is no signicant difference between sexes and sides. The lack of systematic variation in the rate of aging between the sexes was surprising, but allows the same method and age estimates to be applied to both sexes, allowing age to be assessed independently of sex. The revised method generates scores that have a higher correlation with age than the Suchey-Brooks pubic symphysis system for the Spitalelds sample. The age ranges produced by the revised auricular surface method are similar to those given by Brooks and Suchey (1990), regarded as one of the most

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TABLE 12. Age estimates from composite scores and age stages Composite score 56 78 910 1112 1314 1516 1719 Auricular surface stage I II III IV V VI VII No. of specimens 3 6 22 32 64 41 12 Mean age 17.33 29.33 37.86 51.41 59.94 66.71 72.25 Standard deviation 1.53 6.71 13.08 14.47 12.95 11.88 12.73 Median age 17 27 37 52 62 66 73

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Range 1619 2138 1665 2981 2988 3991 5392

TABLE 13. Posterior probability of age, given auricular surface stage, assuming uniform prior probability of age Age 1524 2534 3544 4554 5564 6574 7584 8594 I 0.86 0.14 0.00 0.00 0.00 0.00 0.00 0.00 II 0.33 0.42 0.21 0.04 0.00 0.00 0.00 0.00 III 0.27 0.18 0.20 0.12 0.07 0.02 0.04 0.09 IV 0.00 0.17 0.07 0.16 0.20 0.14 0.20 0.07 V 0.00 0.03 0.19 0.20 0.18 0.26 0.07 0.06 VI 0.00 0.00 0.02 0.04 0.11 0.10 0.27 0.46 VII 0.00 0.00 0.00 0.27 0.00 0.24 0.49 0.00

sexes and potentially to different populations (Konigsberg and Frankenberg, 1992), and shows older age-related changes than the pubic symphysis (Lovejoy et al., 1985). ACKNOWLEDGMENTS We thank Dr. Louise Humphrey of the Natural History Museum, London, for granting us access to the Christ Church Spitalelds skeletal sample. Comments by Dr. Humphrey and Dr. Pia Nystrom of the Department of Archaeology and Prehistory, University of Shefeld, on previous drafts of this paper and the suggestions made by anonymous referees are greatly appreciated. We also thank the osteology students and delegates of the British Association of Biological Anthropology and Osteoarchaeology for taking part in the interobserver error tests, and Alex Norman for drawing the illustration. J.L.B. is currently funded by a White Rose Studentship from the Universities of Shefeld, York, and Leeds.
94) and males (n ST 3 4 4 3 4 3 2 3 2 5 3 3 4 4 4 3 4 3 3 3 4 4 3 3 3 3 3 4 4 86) MI 3 3 3 2 3 3 3 3 3 3 2 1 2 3 3 3 3 3 3 3 3 3 2 3 3 3 2 2 3 MA 1 2 2 2 3 2 1 2 1 3 1 2 1 2 1 1 1 1 1 2 2 2 2 3 2 1 1 2 2 AP 2 1 2 2 2 2 1 2 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 1 2 2 2 (continued)

important and reliable aging methods used at present. This suggests that wide age estimates are more realistic. Narrow age estimates will always remain desirable, but by their nature do not allow for the variation in age-related changes demonstrated by this study. By using narrower age estimates, many individuals could be aged incorrectly, which may hinder identication in forensics cases. This aging technique is important, because the auricular surface is resistant to decay (Waldron, 1987), especially when compared with the fragile pubic symphysis. It is universally applicable to both

APPENDIX. Age and feature scores for known-age females (n Age Females 16 17 19 23 27 27 29 29 30 34 35 35 37 37 38 39 39 41 43 44 45 45 46 47 47 48 49 49 TO 1 2 1 3 1 2 4 2 3 4 2 2 3 3 2 3 5 2 4 3 2 4 4 4 4 2 4 4 ST 1 1 2 2 1 2 3 3 3 3 2 3 3 2 3 2 3 3 3 3 3 3 3 3 4 3 3 3 MI 1 1 1 2 3 2 3 3 2 3 3 3 3 1 3 2 3 3 3 3 2 3 3 3 3 2 2 3 MA 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 1 1 1 1 2 1 2 1 1 3 1 AP 2 1 1 2 1 1 2 2 2 2 1 2 1 1 1 1 3 2 2 2 3 2 2 2 2 2 3 2 Age 50 50 50 52 52 52 53 53 53 53 53 55 55 55 56 56 56 57 57 57 58 59 60 61 61 61 62 63 64 TO 4 4 4 4 4 5 2 4 4 5 2 3 4 4 4 3 4 4 4 2 4 4 4 4 4 3 4 4 3

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APPENDIX. Age and feature scores for known-age females (n 94) and males (n TO 3 3 4 4 5 4 4 4 2 4 4 5 4 5 4 4 4 4 3 2 3 4 4 4 4 4 3 4 3 3 5 5 4 4 4 5 4 4 4 5 4 5 4 5 3 4 4 4 5 4 4 4 4 3 2 4 5 4 3 4 4 4 ST 3 2 3 4 3 3 3 3 3 3 3 4 3 4 4 4 3 3 3 3 4 4 4 4 4 2 3 3 3 4 4 4 4 4 3 3 3 4 3 4 3 5 4 4 4 3 3 3 3 3 4 4 3 3 3 3 4 3 3 3 4 4 86) (Continued) MI 2 3 2 2 2 3 1 3 3 3 3 3 3 3 3 2 3 3 3 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 1 3 3 3 3 3 3 MA 1 1 1 2 1 2 1 1 3 2 1 3 1 1 2 1 1 1 1 1 2 2 3 3 2 1 2 2 2 1 3 2 3 2 1 2 2 1 1 2 3 3 1 3 2 1 2 2 3 1 2 2 2 2 1 1 2 3 1 1 1 3 AP 2 3 2 2 3 3 1 1 2 2 1 2 2 2 2 2 2 2 2 2 1 2 2 2 2 1 2 2 1 3 2 2 3 3 2 2 2 3 2 2 2 2 2 2 2 3 2 2 1 2 2 3 2 2 3 2 2 2 2 2 3 3

Age 65 65 65 65 65 67 67 67 68 68 68 68 70 70 71 72 73 73 74 76 76 76 77 77 78 78 79 79 80 80 81 83 85 85 87 87 89 Males 16 18 21 22 25 26 27 32 32 32 34 34 34 34 36 36 37 38 39 39 39 40 44 45

TO 2 4 4 4 4 3 4 4 3 4 5 4 4 3 4 3 4 3 4 4 4 4 5 5 5 4 5 4 4 4 5 4 4 5 4 3 4 2 4 2 3 2 2 3 3 4 5 2 3 4 4 3 2 2 2 2 2 4 4 2 5

ST 3 4 3 3 3 3 3 4 4 3 4 3 3 3 3 4 2 3 4 3 3 3 5 3 5 4 4 4 4 3 4 4 4 4 4 3 4 2 3 3 3 3 1 2 3 3 3 2 3 2 3 2 3 4 2 2 3 3 3 3 3

MI 2 3 3 3 3 3 3 3 3 3 3 3 3 3 2 3 3 3 3 3 3 2 3 2 3 3 3 3 3 3 3 3 3 3 3 3 3 1 1 1 2 1 3 1 3 3 2 2 2 3 2 3 1 3 2 3 3 3 2 3 3

MA 1 2 1 2 2 2 1 2 1 1 3 2 3 2 2 3 2 3 3 2 2 2 3 1 3 3 2 2 3 1 2 3 3 2 3 3 3 1 1 1 1 1 1 1 1 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2

AP 2 2 2 2 2 1 1 2 2 1 1 2 1 3 3 2 2 2 2 3 1 2 1 2 3 3 2 2 2 2 2 2 3 2 3 2 2 3 1 1 1 2 1 2 2 2 2 2 1 2 1 1 2 2 1 2 1 2 1 1 1

Age 46 47 47 48 49 50 51 51 52 52 53 53 54 56 57 58 58 58 58 58 60 60 60 61 61 62 63 63 63 63 63 64 64 64 65 66 66 66 67 67 68 68 69 69 69 70 70 70 71 71 71 72 72 75 76 77 78 79 81 88 91 92

Abbreviations as in Table 9.

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