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01ffd9}}}}}\pard\sb0\sl-240{\bkmkstart Pg1}{\bkmkend Pg1}\par\pard\ql \li8752\sb
0\sl-310\slmult0 \par\pard\ql\li8752\sb0\sl-310\slmult0 \par\pard\ql\li8752\sb0\
sl-310\slmult0 \par\pard\ql\li8752\sb0\sl-310\slmult0 \par\pard\ql\li8752\sb0\sl
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-310\slmult0 \up0 \expndtw-12\charscalex94 \ul0\nosupersub\cf1\f2\fs27 Lab. Manu
al 6 \par\pard\ql \li3383\sb0\sl-540\slmult0 \par\pard\ql\li3383\sb240\sl-540\sl
mult0 \up0 \expndtw0\charscalex93 \ul0\nosupersub\cf3\f4\fs47 MANUAL OF METHODS
\par\pard\ql \li5675\sb420\sl-540\slmult0 \up0 \expndtw0\charscalex91 OF \par\pa
rd\ql \li3751\sb540\sl-540\slmult0 \up0 \expndtw0\charscalex93 ANALYSIS OF FOODS
\par\pard\ql \li3612\sb0\sl-402\slmult0 \par\pard\ql\li3612\sb0\sl-402\slmult0
\par\pard\ql\li3612\sb0\sl-402\slmult0 \par\pard\ql\li3612\sb0\sl-402\slmult0 \p
ar\pard\ql\li3612\sb226\sl-402\slmult0 \up0 \expndtw0\charscalex90 \ul0\nosupers
ub\cf5\f6\fs35 MEAT AND MEAT PRODUCTS \par\pard\ql \li5613\sb0\sl-402\slmult0 \p
ar\pard\ql\li5613\sb76\sl-402\slmult0 \up0 \expndtw0\charscalex90 AND \par\pard\
ql \li3832\sb0\sl-402\slmult0 \par\pard\ql\li3832\sb96\sl-402\slmult0 \up0 \expn
dtw0\charscalex90 FISH AND FISH PRODUCTS \par\pard\qj \li2148\sb0\sl-460\slmult0
\par\pard\qj\li2148\sb0\sl-460\slmult0 \par\pard\qj\li2148\sb0\sl-460\slmult0 \
par\pard\qj\li2148\sb0\sl-460\slmult0 \par\pard\qj\li2148\ri1866\sb391\sl-460\sl
mult0 \up0 \expndtw0\charscalex97 \ul0\nosupersub\cf7\f8\fs31 DIRECTORATE GENERA
L OF HEALTH SERVICES \line \up0 \expndtw0\charscalex98 MINISTRY OF HEALTH AND FA
MILY WELFARE \par\pard\ql \li3993\sb166\sl-356\slmult0 \up0 \expndtw0\charscalex
97 GOVERNMENT OF INDIA \par\pard\ql \li5073\ri4832\sb32\sl-540\slmult0\tx5577 \u
p0 \expndtw0\charscalex95 NEWDELHI \line\tab \up0 \expndtw0\charscalex96 2005 \p
ar\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart Pg2}{
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4\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\
slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex100 \u
l0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUCTS \pa
r\pard\ql \li1130\sb0\sl-356\slmult0 \par\pard\ql\li1130\sb158\sl-356\slmult0 \u
p0 \expndtw0\charscalex96 \ul0\nosupersub\cf7\f8\fs31 MANUAL FOR ANALYSIS OF MEA
T AND MEAT PRODUCTS \par\pard\ql \li3746\sb4\sl-356\slmult0 \up0 \expndtw0\chars
calex89 AND FISH AND FISH PRODUCTS \par\pard\ql \li4116\sb0\sl-379\slmult0 \par\
pard\ql\li4116\sb6\sl-379\slmult0 \up0 \expndtw0\charscalex99 \ul0\nosupersub\cf
12\f13\fs33 CONTENTS \par\pard\li1130\sb0\sl-310\slmult0\par\pard\li1130\sb16\sl
-310\slmult0\fi4\tx8839 \up0 \expndtw-5\charscalex100 \ul0\nosupersub\cf14\f15\f
s27 1.0 Meat and Meat Products\tab \up0 \expndtw-5\charscalex100 \ul0\nosupersub
\cf1\f2\fs27 1\par\pard\li1130\sb0\sl-310\slmult0\par\pard\li1130\sb42\sl-310\sl
mult0\fi0\tx8834 \up0 \expndtw-5\charscalex100 1,1 Preparation of Sample\tab \up
0 \expndtw-5\charscalex100 1\par\pard\li1130\sb4\sl-310\slmult0\fi0\tx8826 \up0
\expndtw-5\charscalex100 1.2 Determination of Nitrite\tab \up0 \expndtw-5\charsc
alex100 3\par\pard\li1130\sb7\sl-310\slmult0\fi0\tx8858 \up0 \expndtw-5\charscal
ex100 1.2. A - Alternate method for Determination of Nitrite\tab \up0 \expndtw5\charscalex100 5\par\pard\li1130\sb2\sl-310\slmult0\fi0\tx8858 \up0 \expndtw-5\
charscalex100 1 .3 - Determination of Ascorbic acid\tab \up0 \expndtw-5\charscal

ex100 8\par\pard\li1130\sb5\sl-310\slmult0\fi0\tx8858 \up0 \expndtw-5\charscalex

100 1.3 A - Alternate method for Determination of Ascorbic acid\tab \up0 \expndt
w-5\charscalex100 11\par\pard\li1130\sb4\sl-310\slmult0\fi0\tx8858 \up0 \expndtw
-5\charscalex100 1.4 Determination of Total Phosphorous\tab \up0 \expndtw-5\char
scalex100 11\par\pard\li1130\sb4\sl-310\slmult0\fi0\tx8858 \up0 \expndtw-5\chars
calex100 1.5 Test for presence of Polyphosphates\tab \up0 \expndtw-5\charscalex1
00 15\par\pard\li1130\sb2\sl-310\slmult0\fi0\tx8858 \up0 \expndtw-5\charscalex10
0 1.6 - Determination of Glucono - delta - lactone\tab \up0 \expndtw-5\charscale
x100 18\par\pard\li1130\sb5\sl-310\slmult0\fi45\tx8858 \up0 \expndtw-5\charscale
x100 2..0 - Additional tests\tab \up0 \expndtw-5\charscalex100 21\par\pard\li113
0\sb2\sl-310\slmult0\fi0\tx8858 \up0 \expndtw-5\charscalex100 2.1-Total Fat\tab
\up0 \expndtw-5\charscalex100 21\par\pard\li1130\sb2\sl-310\slmult0\fi0\tx8858 \
up0 \expndtw-5\charscalex100 2.2 - Total Protein\tab \up0 \expndtw-5\charscalex1
00 22\par\pard\li1130\sb0\sl-310\slmult0\par\pard\li1130\sb9\sl-310\slmult0\fi0
\up0 \expndtw-5\charscalex100 \ul0\nosupersub\cf14\f15\fs27 Fish and Fish Produc
ts\par\pard\li1130\sb0\sl-310\slmult0\par\pard\li1130\sb35\sl-310\slmult0\fi9\tx
8896 \up0 \expndtw-5\charscalex100 \ul0\nosupersub\cf1\f2\fs27 1 .0 -\ul0\nosupe
rsub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Frozen Fish\tab \up0 \expndtw-5\
charscalex100 24\par\pard\li1130\sb2\sl-310\slmult0\fi9\tx8896 \up0 \expndtw-5\c
harscalex100 1 .1 -\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Pr
eparation of Sample\tab \up0 \expndtw-5\charscalex100 24\par\pard\li1130\sb4\sl310\slmult0\fi9\tx8896 \up0 \expndtw-5\charscalex100 1 .2 -\ul0\nosupersub\cf15\
f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Determination of Total Volatile Bases\tab
\up0 \expndtw-5\charscalex100 25\par\pard\li1130\sb2\sl-310\slmult0\fi9\tx8896 \
up0 \expndtw-5\charscalex100 1 .3 -\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersu
b\cf1\f2\fs27 Determination of Histamine\tab \up0 \expndtw-5\charscalex100 26\pa
r\pard\li1130\sb2\sl-310\slmult0\fi9\tx8927 \up0 \expndtw-5\charscalex100 1.3 .A
- Alternate method for Determination of Histamine\tab \up0 \expndtw-5\charscale
x100 28\par\pard\li1130\sb7\sl-310\slmult0\fi9\tx8920 \up0 \expndtw-5\charscalex
100 2.0 - Dried Fish\tab \up0 \expndtw-5\charscalex100 33\par\pard\li1130\sb2\sl
-310\slmult0\fi9\tx8913 \up0 \expndtw-5\charscalex100 2.1-Sampling\tab \up0 \exp
ndtw-5\charscalex100 33\par\pard\li1130\sb26\sl-310\slmult0\fi0\tx8917 \up0 \exp
ndtw-5\charscalex100 2.2\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs
27 - Determination of Moisture\tab \up0 \expndtw-5\charscalex100 34\par\pard\li1
130\sb4\sl-310\slmult0\fi0\tx8918 \up0 \expndtw-5\charscalex100 2.3\ul0\nosupers
ub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 - Determination of Sodium Chloride
\tab \up0 \expndtw-5\charscalex100 34\par\pard\li1130\sb2\sl-310\slmult0\fi0\tx8
918 \up0 \expndtw-5\charscalex100 2.4\ul0\nosupersub\cf15\f16\fs27 \ul0\nosuper
sub\cf1\f2\fs27 - Determination of Ash insoluble in dil Hydrochloric acid\tab \u
p0 \expndtw-5\charscalex100 36\par\pard\li1130\sb5\sl-310\slmult0\fi0\tx8978 \up
0 \expndtw-5\charscalex100 3.0 -Canned Fish\tab \up0 \expndtw-5\charscalex100 37
\par\pard\li1130\sb26\sl-310\slmult0\fi9\tx8939 \up0 \expndtw-5\charscalex100 3
.1 -\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Preparation of sa
mple\tab \up0 \expndtw-5\charscalex100 37\par\pard\li1130\sb2\sl-310\slmult0\fi9
\tx8887 \up0 \expndtw-5\charscalex100 3 .2 -\ul0\nosupersub\cf15\f16\fs27 \ul0\
nosupersub\cf1\f2\fs27 Determination of Acidity of Brine\tab \up0 \expndtw-5\cha
rscalex100 37\par\pard\li1130\sb4\sl-310\slmult0\fi9\tx8927 \up0 \expndtw-5\char
scalex100 3 .3 -\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Modif
ied Starches in packing medium\tab \up0 \expndtw-5\charscalex100 38\par\pard\li1
130\sb2\sl-310\slmult0\fi9\tx8952 \up0 \expndtw-5\charscalex100 3.4-\ul0\nosuper
sub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Determination of Ascorbic acid\t
ab \up0 \expndtw-5\charscalex100 39\par\pard\li1130\sb4\sl-310\slmult0\fi9\tx894
9 \up0 \expndtw-5\charscalex100 3 .5 -\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupe
rsub\cf1\f2\fs27 Determination of Polyphosphates\tab \up0 \expndtw-5\charscalex1
00 39\par\pard\li1130\sb5\sl-310\slmult0\fi9\tx8978 \up0 \expndtw-5\charscalex10
0 3 .6 -\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Determination
of Sulphur Dioxide\tab \up0 \expndtw-5\charscalex100 39\par\pard\ql \li5918\sb0
\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb0\s
l-218\slmult0 \par\pard\ql\li5918\sb182\sl-218\slmult0 \up0 \expndtw-2\charscale
x100 \ul0\nosupersub\cf10\f11\fs19 1

{\shp {\*\shpinst\shpleft1104\shptop2128\shpright10828\shpbottom2485\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 357}}
{\sp{\sn pVerticies}{\sv 8;4;(0,357);(9724,357);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1130\shptop2449\shpright9902\shpbottom2480\shpfhdr0\shp
bxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz52\shplid1
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8772}}{\sp{\sn geoBottom}{\sv 31}}
{\sp{\sn pVerticies}{\sv 8;4;(0,31);(8772,31);(8772,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 0}}{\s
p{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv 1}}{\s
p{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop2485\shpright10828\shpbottom2845\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz62\shplid2
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 360}}
{\sp{\sn pVerticies}{\sv 8;4;(0,360);(9724,360);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft3746\shptop2807\shpright8184\shpbottom2838\shpfhdr0\shp
bxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz76\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 4438}}{\sp{\sn geoBottom}{\sv 31}}
{\sp{\sn pVerticies}{\sv 8;4;(0,31);(4438,31);(4438,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 0}}{\s
p{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv 1}}{\s
p{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop2845\shpright10828\shpbottom3224\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz85\shplid4
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 379}}
{\sp{\sn pVerticies}{\sv 8;4;(0,379);(9724,379);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft3434\shptop3224\shpright10828\shpbottom3604\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz95\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 7394}}{\sp{\sn geoBottom}{\sv 380}}
{\sp{\sn pVerticies}{\sv 8;4;(0,380);(7394,380);(7394,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1106\shptop3604\shpright10828\shpbottom3920\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz114\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9722}}{\sp{\sn geoBottom}{\sv 316}}
{\sp{\sn pVerticies}{\sv 8;4;(0,316);(9722,316);(9722,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1106\shptop3920\shpright10828\shpbottom4232\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz124\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9722}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9722,312);(9722,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop4578\shpright10828\shpbottom4890\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz159\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9724,312);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop4890\shpright10828\shpbottom5204\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz177\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9724,314);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1104\shptop5204\shpright10828\shpbottom5521\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz197\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 317}}
{\sp{\sn pVerticies}{\sv 8;4;(0,317);(9724,317);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop5521\shpright10828\shpbottom5836\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz232\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 315}}
{\sp{\sn pVerticies}{\sv 8;4;(0,315);(9724,315);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop5836\shpright10828\shpbottom6150\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz268\shplid12
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9724,314);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop6150\shpright10828\shpbottom6464\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz308\shplid13
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9724,314);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop6464\shpright10828\shpbottom6776\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz331\shplid14
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9724,312);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1104\shptop6776\shpright10828\shpbottom7088\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz353\shplid15
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9724,312);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop7088\shpright10828\shpbottom7403\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz400\shplid16
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 315}}
{\sp{\sn pVerticies}{\sv 8;4;(0,315);(9724,315);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop7403\shpright10828\shpbottom7715\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz435\shplid17
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9724,312);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop7715\shpright10828\shpbottom8032\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz464\shplid18
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 317}}
{\sp{\sn pVerticies}{\sv 8;4;(0,317);(9724,317);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop8032\shpright10828\shpbottom8344\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz494\shplid19
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9724,312);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1104\shptop8344\shpright10828\shpbottom8656\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz502\shplid20
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9724,312);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop8999\shpright10828\shpbottom9311\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9717}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9717,312);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1111\shptop9311\shpright10828\shpbottom9623\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz564\shplid22
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9717,312);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1111\shptop9623\shpright10828\shpbottom9937\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft1111\shptop9937\shpright10828\shpbottom10249\shpfhdr0\s
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9717,312);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1111\shptop10249\shpright10828\shpbottom10566\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz687\shplid25
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9717}}{\sp{\sn geoBottom}{\sv 317}}
{\sp{\sn pVerticies}{\sv 8;4;(0,317);(9717,317);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1111\shptop10566\shpright10828\shpbottom10878\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9717,312);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1111\shptop10878\shpright10828\shpbottom11192\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9717,314);(9717,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop11214\shpright10828\shpbottom11528\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz772\shplid28
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9724,314);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1104\shptop11528\shpright10828\shpbottom11840\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9724,312);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1104\shptop11840\shpright10828\shpbottom12155\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz845\shplid30
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 315}}
{\sp{\sn pVerticies}{\sv 8;4;(0,315);(9724,315);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1104\shptop12155\shpright10828\shpbottom12467\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9724,312);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop12491\shpright10828\shpbottom12803\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9717,312);(9717,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop12803\shpright10828\shpbottom13117\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz951\shplid33
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9717}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9717,314);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop13117\shpright10828\shpbottom13429\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz988\shplid34
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9717}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9717,312);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1111\shptop13429\shpright10828\shpbottom13741\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz1029\shplid35
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9717}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9717,312);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop13741\shpright10828\shpbottom14058\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,317);(9717,317);(9717,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1111\shptop14058\shpright10828\shpbottom14370\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz1103\shplid37
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9717,312);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g3}{\bkmkend Pg3}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex10
0 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUCTS
\par\pard\ql \li1130\sb0\sl-356\slmult0 \par\pard\ql\li1130\sb0\sl-356\slmult0
\par\pard\ql\li1130\sb202\sl-356\slmult0 \up0 \expndtw0\charscalex96 \ul0\nosupe
rsub\cf7\f8\fs31 MANUAL FOR ANALYSIS OF MEAT AND MEAT PRODUCTS \par\pard\ql \li3
746\sb4\sl-356\slmult0 \up0 \expndtw0\charscalex89 AND FISH AND FISH PRODUCTS \p
ar\pard\qj \li1130\sb0\sl-473\slmult0 \par\pard\qj\li1130\sb0\sl-473\slmult0 \pa
r\pard\qj\li1130\sb0\sl-473\slmult0 \par\pard\qj\li1130\sb0\sl-473\slmult0 \par\
pard\qj\li1130\ri882\sb96\sl-473\slmult0 \up0 \expndtw0\charscalex100 \ul0\nosup
ersub\cf1\f2\fs27 Meat, fish and their products are important components of diet
of a large majority of \line \up0 \expndtw0\charscalex103 people. Their nutriti
ve value and palatability are widely appreciated. Standards for \line \up0 \expn
dtw-3\charscalex100 meat and meat products and fish and fish products are laid d
own in item A 34 and A 35 \line \up0 \expndtw-4\charscalex100 respectively of
Appendix B to P.F.A Rules \up0 \expndtw-4\charscalex100 (1955). These stan
dards contain \par\pard\qj \li1130\ri862\sb18\sl-460\slmult0 \up0 \expndtw-2\ch
arscalex100 microbiological requirements in addition to chemical requirements. A
separate manual \up0 \expndtw-3\charscalex100 has been prepared for microbiolog
ical examination of these products \par\pard\ql \li1149\sb0\sl-356\slmult0 \par\
pard\ql\li1149\sb0\sl-356\slmult0 \par\pard\ql\li1149\sb94\sl-356\slmult0 \up0 \
expndtw0\charscalex91 \ul0\nosupersub\cf7\f8\fs31 1.0 Meat and Meat Products \pa

r\pard\ql \li1149\sb0\sl-356\slmult0 \par\pard\ql\li1149\sb0\sl-356\slmult0 \par

\pard\ql\li1149\sb72\sl-356\slmult0 \up0 \expndtw0\charscalex90 1.1 Preparation
of sample: -\par\pard\qj \li1276\ri1015\sb210\sl-470\slmult0 \up0 \expndtw-1\cha
rscalex100 \ul0\nosupersub\cf1\f2\fs27 To prevent loss of moisture during prepar
ation and subsequent handling, do not use \up0 \expndtw-1\charscalex100 small te
st samples. Keep ground material in glass or similar containers with air and \up
0 \expndtw-4\charscalex100 water tight covers \par\pard\ql \li1276\sb0\sl-310\sl
mult0 \par\pard\ql\li1276\sb293\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul
0\nosupersub\cf14\f15\fs27 (a) Fresh and frozen meat, cured meats, smoked meats
etc \par\pard\qj \li1272\ri1019\sb470\sl-480\slmult0 \up0 \expndtw-1\charscalex1
00 \ul0\nosupersub\cf1\f2\fs27 Separate as completely as possible from any bone,
pass rapidly three times through \line \up0 \expndtw-4\charscalex100 food chopp
er with plate opening equal to 1/8th\ul0\nosupersub\cf16\f17\fs27 \ul0\nosupers
ub\cf1\f2\fs27 inch ( 3 mm), mixing thoroughly after \par\pard\ql \li5918\sb0\sl
-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-2
18\slmult0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb25\sl-21
8\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf10\f11\fs19 1
{\shp {\*\shpinst\shpleft2654\shptop2128\shpright10828\shpbottom2528\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,400);(8174,400);(8174,0);(0,0)}}
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}}
{\shp {\*\shpinst\shpleft1104\shptop2528\shpright10828\shpbottom2888\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,360);(9724,360);(9724,0);(0,0)}}
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}}
{\shp {\*\shpinst\shpleft1130\shptop2852\shpright9902\shpbottom2881\shpfhdr0\shp
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 0}}{\s
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}}
{\shp {\*\shpinst\shpleft1104\shptop2888\shpright10828\shpbottom3246\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1104\shptop4480\shpright10828\shpbottom5713\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1248\shptop12575\shpright10828\shpbottom12887\shpfhdr0\
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{\shp {\*\shpinst\shpleft1243\shptop13384\shpright10694\shpbottom13849\shpfhdr0\
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{\shp {\*\shpinst\shpleft1243\shptop13849\shpright10694\shpbottom14317\shpfhdr0\
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g4}{\bkmkend Pg4}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex10
0 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUCTS
\par\pard\qj \li1272\sb0\sl-465\slmult0 \par\pard\qj\li1272\ri1019\sb59\sl-465\
slmult0 \up0 \expndtw0\charscalex106 \ul0\nosupersub\cf1\f2\fs27 each grinding a
nd begin all determinations promptly. If any delay occurs chill \up0 \expndtw0\c
harscalex105 sample to inhibit decomposition In case of cured meats, mix thoroug
hly with a \up0 \expndtw0\charscalex106 spatula or pass through a food chopper o
r mix in a homogeniser / blender to a \up0 \expndtw0\charscalex107 uniform mass
as appropriate. Transfer to a wide mouth glass or other suitable \up0 \expndtw4\charscalex100 container with a airtight stopper. Carry out analysis as soon as
possible \par\pard\ql \li1144\sb0\sl-310\slmult0 \par\pard\ql\li1144\sb14\sl-31
0\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 (b) Canned
meats \par\pard\qj \li1144\sb0\sl-480\slmult0 \par\pard\qj\li1144\ri945\sb150\s
l-480\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Pass ent
ire contents of the can through the food chopper or blender to obtain a uniform
\up0 \expndtw-5\charscalex100 mass. \par\pard\ql \li1130\sb0\sl-468\slmult0 \par
\pard\ql\li1130\ri1137\sb2\sl-468\slmult0 \up0 \expndtw-4\charscalex100 Dry port
ions of (a) and (b) not needed for immediate analysis either in vacuo at less \l
ine \up0 \expndtw-3\charscalex100 than 60 C or by evaporating on steam bath 2 -3
times with alcohol. Extract fat from \up0 \expndtw-2\charscalex100 dried produc
t with petroleum ether (b. p. less than 60 C) and let petroleum ether \line \up0
\expndtw-5\charscalex100 evaporate spontaneously, finally expelling last traces
by heating short time on steam \line \up0 \expndtw-4\charscalex100 bath. Do not
heat test sample or separated fat longer than necessary because of \line \up0 \
expndtw-5\charscalex100 tendency to decompose. \par\pard\qj \li1140\sb0\sl-480\s
lmult0 \par\pard\qj\li1140\sb0\sl-480\slmult0 \par\pard\qj\li1140\sb0\sl-480\slm
ult0 \par\pard\qj\li1140\sb0\sl-480\slmult0 \par\pard\qj\li1140\ri889\sb403\sl-4
80\slmult0 \up0 \expndtw-1\charscalex100 (Ref:- A.O.A.C 17\ul0\super\cf17\f18\fs
26 th\ul0\nosupersub\cf1\f2\fs27 edition,2000, 983.18 Meat and Meat Products, P
reparation of test \up0 \expndtw-3\charscalex100 sample (a)and (b)) \par\pard\ql
\li1154\sb0\sl-356\slmult0 \par\pard\ql\li1154\sb0\sl-356\slmult0 \par\pard\ql\
li1154\sb11\sl-356\slmult0 \up0 \expndtw0\charscalex96 \ul0\nosupersub\cf7\f8\fs
31 1.2 Determination of Nitrite (rule 55) \par\pard\ql \li5918\sb0\sl-218\slmult
0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\slmult0
\par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb26\sl-218\slmult0 \
up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf10\f11\fs19 2
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{\shp {\*\shpinst\shpleft1116\shptop4463\shpright10828\shpbottom4777\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz144\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

\sn geoRight}{\sv 9712}}{\sp{\sn geoBottom}{\sv 314}}

{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9712,314);(9712,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1116\shptop4777\shpright10828\shpbottom5245\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz153\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9712}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9712,468);(9712,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1116\shptop5737\shpright10816\shpbottom6208\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz168\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9700}}{\sp{\sn geoBottom}{\sv 471}}
{\sp{\sn pVerticies}{\sv 8;4;(0,471);(9700,471);(9700,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1116\shptop6208\shpright10816\shpbottom6683\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz187\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9700}}{\sp{\sn geoBottom}{\sv 475}}
{\sp{\sn pVerticies}{\sv 8;4;(0,475);(9700,475);(9700,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop7156\shpright10828\shpbottom7621\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz202\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9724,465);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop7621\shpright10828\shpbottom8089\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz213\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}

{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop8089\shpright10828\shpbottom8557\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz239\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop8557\shpright10828\shpbottom9023\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz265\shplid12
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9724,466);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop9023\shpright10828\shpbottom9491\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz279\shplid13
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop9491\shpright10828\shpbottom9959\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz293\shplid14
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop10897\shpright10821\shpbottom11365\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz312\shplid15
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

\sn geoRight}{\sv 9710}}{\sp{\sn geoBottom}{\sv 468}}

{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9710,468);(9710,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop11365\shpright10821\shpbottom12772\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz322\shplid16
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9710}}{\sp{\sn geoBottom}{\sv 1407}}
{\sp{\sn pVerticies}{\sv 8;4;(0,1407);(9710,1407);(9710,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop12772\shpright10821\shpbottom13237\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz371\shplid17
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9710}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9710,465);(9710,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1125\shptop13621\shpright9520\shpbottom14406\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz385\shplid18
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8395}}{\sp{\sn geoBottom}{\sv 785}}
{\sp{\sn pVerticies}{\sv 8;4;(0,785);(8395,785);(8395,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g5}{\bkmkend Pg5}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex10
0 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUCTS
\par\pard\ql \li1154\sb0\sl-310\slmult0 \par\pard\ql\li1154\sb0\sl-310\slmult0
\par\pard\ql\li1154\sb272\sl-310\slmult0 \up0 \expndtw0\charscalex104 \ul0\nosup
ersub\cf14\f15\fs27 1.2.1 Reagents:-\par\pard\li1135\sb0\sl-310\slmult0\par\pard
\li1135\sb0\sl-310\slmult0\par\pard\li1135\sb10\sl-310\slmult0\fi0\tx3516\tx3927
\tx5204\tx5867\tx7142\tx7690 \up0 \expndtw0\charscalex104 \ul0\nosupersub\cf1\f2
\fs27 (a)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 NED reagen
t\tab \up0 \expndtw0\charscalex104 -\tab \up0 \expndtw0\charscalex104 Dissolve\t
ab \up0 \expndtw0\charscalex104 0.2\tab \up0 \expndtw0\charscalex104 gm N-\tab
\up0 \expndtw0\charscalex104 (1\tab \up0 \expndtw0\charscalex104 Napthyl) eth
ylenediamine\par\pard\li1135\sb166\sl-310\slmult0\fi0\tx3552\tx4780\tx5512 \up0
\expndtw0\charscalex104 dihydrochloride in\tab \up0 \expndtw0\charscalex104 150

ml,\tab \up0 \expndtw0\charscalex104 15%\tab \up0 \expndtw0\charscalex104 (v/v

) acetic acid. Filter if necessary and\par\pard\ql \li1135\sb154\sl-310\sl
mult0 \up0 \expndtw-4\charscalex100 store in a glass stoppered brown glass bottl
e. \par\pard\qj \li1135\sb0\sl-470\slmult0 \par\pard\qj\li1135\ri2813\sb28\sl-47
0\slmult0 \up0 \expndtw-6\charscalex100 (b)\ul0\nosupersub\cf15\f16\fs27 \ul0\n
osupersub\cf1\f2\fs27 Sulphanilamide reagent- Dissolve 0.5gm sulphanilamide in 1
50 ml \up0 \expndtw-4\charscalex100 15% acetic acid (v/v). Filter, if necessary
and store in a glass stoppered \up0 \expndtw-5\charscalex100 brown bottle. \par\
pard\ql \li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb173\sl-310\slmult0 \up0
\expndtw-4\charscalex100 (c) Nitrite standard solution-\par\pard\qj \li1144\sb0
\sl-480\slmult0 \par\pard\qj\li1144\ri2751\sb10\sl-480\slmult0 \up0 \expndtw-5\c
harscalex100 (i) Stock solution - 1000 ppm. NaNO\ul0\sub\cf17\f18\fs26 2\ul0\nos
upersub\cf1\f2\fs27 - Dissolve l.000 gm pure NaNO\ul0\sub\cf17\f18\fs26 2 \up0
\expndtw-5\charscalex100 \ul0\nosupersub\cf1\f2\fs27 in water and make upto 1 li
tre. \par\pard\qj \li1140\ri2543\sb0\sl-480\slmult0 \up0 \expndtw-3\charscalex10
0 (ii) Intermediate solution - 100 ppm. - Dilute 100ml of stock solution to \up0
\expndtw-6\charscalex100 1 litre with water. \par\pard\qj \li1135\ri2711\sb0\sl
-480\slmult0\fi4 \up0 \expndtw-4\charscalex100 (iii) Working solution- lppm - Di
lute 10ml of intermediate sol to 1 litre \up0 \expndtw-5\charscalex100 with wate
r. \par\pard\ql \li1130\ri870\sb432\sl-466\slmult0 \up0 \expndtw-2\charscalex100
(d) Filter paper - Test for nitrite contamination by analyzing 3-4 sheets at ra
ndom. Filter \up0 \expndtw-4\charscalex100 approx 40ml water through each sheet.
Add 4ml of Sulphanilamide reagent, mix, let \line \up0 \expndtw-3\charscalex100
stand 5 minutes, add 4ml of NED reagent, mix and wait for 15 minutes. If any sh
eets are \up0 \expndtw-4\charscalex100 positive do not use them. \par\pard\ql \l
i1144\sb0\sl-310\slmult0 \par\pard\ql\li1144\sb293\sl-310\slmult0 \up0 \expndtw4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.2.2 Procedure:-\par\pard\ql \li5
918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li591
8\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\
sb0\sl-218\slmult0 \par\pard\ql\li5918\sb156\sl-218\slmult0 \up0 \expndtw-2\char
scalex100 \ul0\nosupersub\cf10\f11\fs19 3
{\shp {\*\shpinst\shpleft1125\shptop2128\shpright7298\shpbottom2912\shpfhdr0\shp
bxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 6173}}{\sp{\sn geoBottom}{\sv 784}}
{\sp{\sn pVerticies}{\sv 8;4;(0,784);(6173,784);(6173,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1106\shptop3306\shpright10828\shpbottom3776\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9722}}{\sp{\sn geoBottom}{\sv 470}}
{\sp{\sn pVerticies}{\sv 8;4;(0,470);(9722,470);(9722,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1106\shptop3776\shpright10828\shpbottom4249\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9722}}{\sp{\sn geoBottom}{\sv 473}}

{\sp{\sn pVerticies}{\sv 8;4;(0,473);(9722,473);(9722,0);(0,0)}}

{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1106\shptop4249\shpright10828\shpbottom4720\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz143\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9722}}{\sp{\sn geoBottom}{\sv 471}}
{\sp{\sn pVerticies}{\sv 8;4;(0,471);(9722,471);(9722,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1106\shptop4720\shpright10828\shpbottom5192\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz161\shplid4
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9722}}{\sp{\sn geoBottom}{\sv 472}}
{\sp{\sn pVerticies}{\sv 8;4;(0,472);(9722,472);(9722,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft1106\shptop5192\shpright10828\shpbottom5665\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz169\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9722}}{\sp{\sn geoBottom}{\sv 473}}
{\sp{\sn pVerticies}{\sv 8;4;(0,473);(9722,473);(9722,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft1106\shptop5665\shpright10828\shpbottom6136\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,471);(9722,471);(9722,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1106\shptop6136\shpright10828\shpbottom6608\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz221\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,472);(9722,472);(9722,0);(0,0)}}

{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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}}
{\shp {\*\shpinst\shpleft1104\shptop6608\shpright10828\shpbottom7079\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz235\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,471);(9724,471);(9724,0);(0,0)}}
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{\shp {\*\shpinst\shpleft1104\shptop7079\shpright10828\shpbottom7396\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz243\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,317);(9724,317);(9724,0);(0,0)}}
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{\shp {\*\shpinst\shpleft1116\shptop7895\shpright10828\shpbottom8363\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz266\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1116\shptop8363\shpright10828\shpbottom8833\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz320\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,470);(9712,470);(9712,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1111\shptop8838\shpright10828\shpbottom9306\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz336\shplid12
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9717,468);(9717,0);(0,0)}}

{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1135\shptop9306\shpright10828\shpbottom9774\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz368\shplid13
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9693,468);(9693,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1111\shptop9779\shpright10828\shpbottom10244\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz384\shplid14
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9717,465);(9717,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1106\shptop10249\shpright10828\shpbottom10717\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz416\shplid15
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9722,468);(9722,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1104\shptop11195\shpright10828\shpbottom11660\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9724,465);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1104\shptop11660\shpright10828\shpbottom12128\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}

{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1104\shptop12128\shpright10828\shpbottom12596\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1104\shptop12596\shpright10828\shpbottom13062\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9724,466);(9724,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft1116\shptop13660\shpright10828\shpbottom13974\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz514\shplid20
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9712,314);(9712,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g6}{\bkmkend Pg6}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex10
0 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUCTS
\par\pard\qj \li1130\sb0\sl-466\slmult0 \par\pard\qj\li1130\ri882\sb58\sl-466\s
lmult0 \up0 \expndtw0\charscalex103 \ul0\nosupersub\cf1\f2\fs27 Weigh 5 gms prep
ared sample in a 50 ml beaker. Add about 40ml of water heated to \line \up0 \exp
ndtw0\charscalex106 80C. Mix thoroughly with glass rod taking care to break all l
umps and transfer to \line \up0 \expndtw0\charscalex100 500ml volumetric flask
. Thoroughly wash beaker and glass rod with successive \line \up0 \expnd
tw-2\charscalex100 portions of hot water adding all washings to flask. Add enoug
h hot water to bring vol to \line \up0 \expndtw0\charscalex102 about 300ml. Tran
sfer flask to steam bath and let stand 2 hours shaking occasionally. \line \up0
\expndtw0\charscalex103 Cool to room temperature, dilute to volume with water an
d remix. Filter. If turbidity \line \up0 \expndtw-1\charscalex100 remains after
filtration, centrifuging will usually clear the solution. Add \up0 \exp

ndtw0\charscalex103 2.5ml of \par\pard\qj \li1130\ri869\sb0\sl-470\slmult0 \up0

\expndtw0\charscalex103 sulphanilamide sol to aliquot containing 5-50 ug NaNO2 i
n 50 ml vol flask and mix. \line \up0 \expndtw0\charscalex100 After 5 minutes ad
d 2.5 ml NED reagent, mix dilute to vol, mix and let colour develop \line \up0 \
expndtw-2\charscalex100 15 minutes. Transfer portion of solution to photometer c
ell and determine absorbance at \line \up0 \expndtw0\charscalex100 540 nm agains
t blank of 45ml water and 2.5ml of sulphanilamide reagent and 2.5ml of \line \up
0 \expndtw-1\charscalex100 NED reagent. \par\pard\qj \li1135\ri2528\sb0\sl-460\s
lmult0 \up0 \expndtw-3\charscalex100 Determine Nitrite present by comparison wit
h standard curve prepared as \up0 \expndtw-4\charscalex100 follows:-\par\pard\ql
\li1135\sb0\sl-470\slmult0 \par\pard\ql\li1135\ri1495\sb12\sl-470\slmult0\fi4 \
up0 \expndtw-3\charscalex100 Add 10, 20, 30, 40 ml of nitrite working solution t
o 50ml vol flasks. Add 2.5 ml of \up0 \expndtw-3\charscalex100 sulphanilamide re
agent and after 5 minutes add 2.5ml of NED \line \up0 \expndtw-3\charscalex100 r
eagent and proceed as above. \par\pard\ql \li1149\sb123\sl-310\slmult0 \up0 \exp
ndtw-3\charscalex100 Standard curve is straight line upto 1 ppm Na NO\ul0\sub\cf
17\f18\fs26 2\ul0\nosupersub\cf1\f2\fs27 in final solution \par\pard\qj \li1135
\sb0\sl-460\slmult0 \par\pard\qj\li1135\sb0\sl-460\slmult0 \par\pard\qj\li1135\s
b0\sl-460\slmult0 \par\pard\qj\li1135\ri1216\sb87\sl-460\slmult0 \up0 \expndtw0\
charscalex100 (Ref :- A.O.A.C Official method 17\ul0\super\cf17\f18\fs26 th\ul0\
nosupersub\cf1\f2\fs27 edition 2000, 973.31 Nitrites in cured meats -\line \up0
\expndtw0\charscalex100 Colorimetric method, Adopted as Codex Reference method
(Type II)) \par\pard\ql \li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb0\sl-31
0\slmult0 \par\pard\ql\li1149\sb4\sl-310\slmult0 \up0 \expndtw-6\charscalex100 \
ul0\nosupersub\cf14\f15\fs27 1.2 A Alternate method for determination of Nitrite
\par\pard\ql \li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\slmult0
\par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \p
ar\pard\ql\li5918\sb34\sl-218\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosuper
sub\cf10\f11\fs19 4
{\shp {\*\shpinst\shpleft1104\shptop2128\shpright10828\shpbottom2596\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1104\shptop2596\shpright10828\shpbottom3061\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9724,465);(9724,0);(0,0)}}
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{\shp {\*\shpinst\shpleft1104\shptop3061\shpright10828\shpbottom3529\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1104\shptop3529\shpright10828\shpbottom3997\shpfhdr0\sh
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}}
{\shp {\*\shpinst\shpleft1104\shptop3997\shpright10828\shpbottom4463\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1104\shptop4463\shpright10828\shpbottom4931\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1104\shptop4931\shpright10828\shpbottom5399\shpfhdr0\sh
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g7}{\bkmkend Pg7}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex10
0 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUCTS
\par\pard\ql \li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb0\sl-310\slmult0
\par\pard\ql\li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb82\sl-310\slmult0 \
up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.2.1. A Principle \p
ar\pard\ql \li1130\sb0\sl-466\slmult0 \par\pard\ql\li1130\ri1172\sb36\sl-466\slm
ult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Extraction of a t
est portion in hot water, precipitation of the proteins and filtration. In \up0
\expndtw-2\charscalex100 the presence of nitrite development of a red colour by
the addition of sulphanilamide \up0 \expndtw-2\charscalex100 and N - napthylethy
lenediamine dihydrochloride to the filtrate and photometric \line \up0 \expndtw3\charscalex100 measurement at 538 nm \par\pard\ql \li1149\sb0\sl-310\slmult0 \p
ar\pard\ql\li1149\sb293\sl-310\slmult0 \up0 \expndtw-5\charscalex100 \ul0\nosupe
rsub\cf14\f15\fs27 1.2.2 A\ul0\nosupersub\cf1\f2\fs27 \ul0\nosupersub\cf14\f15\
fs27 Reagents \par\pard\ql \li1140\sb0\sl-310\slmult0 \par\pard\ql\li1140\sb0\sl
-310\slmult0 \par\pard\ql\li1140\sb10\sl-310\slmult0 \up0 \expndtw-4\charscalex1
00 \ul0\nosupersub\cf1\f2\fs27 (a) Solutions for precipitation of proteins \par\
pard\qj \li1845\ri2602\sb0\sl-480\slmult0\tx2224 \up0 \expndtw-5\charscalex100 (
1)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Dissolve 106 gm of
Potassium ferrocyanide trihydrate in water \line\tab \up0 \expndtw-5\charscalex1
00 and dilute to 1000 ml \par\pard\qj \li1845\ri2874\sb0\sl-460\slmult0\tx2224 \
up0 \expndtw-5\charscalex100 (2)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\c
f1\f2\fs27 Dissolve 220 gm of Zinc acetate dihydrate and 30 ml glacial \line\tab
\up0 \expndtw-5\charscalex100 acetic acid in water and dilute to 1000 ml \par\p
ard\qj \li1845\ri2509\sb0\sl-480\slmult0\tx2224 \up0 \expndtw-5\charscalex100 (3
)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Dissolve 50 gm of di
sodium tetraborate decahydrate in 1000 ml \line\tab \up0 \expndtw-5\charscalex10
0 of tepid water and cool to room temperature \par\pard\li1135\sb0\sl-310\slmult
0\par\pard\li1135\sb285\sl-310\slmult0\fi0\tx6033\tx7668 \up0 \expndtw0\charscal
ex104 (b) Standard Sodium nitrite solution\tab \up0 \expndtw0\charscalex10
4 - Dissolve\tab \up0 \expndtw0\charscalex104 1.000 gm pure sodium\par\p
ard\li1135\sb155\sl-310\slmult0\fi364\tx5318\tx10193 \up0 \expndtw0\charscalex10
4 nitrite in water and dilute to\tab \up0 \expndtw0\charscalex104 100 ml
in a volumetric flask. Pipette\tab \up0 \expndtw0\charscalex104 5 ml\par\pa
rd\ql \li1500\sb142\sl-310\slmult0 \up0 \expndtw-4\charscalex100 of the solution
into a 1000 ml volumetric flask and make upto volume. \par\pard\ql \li1130\sb1
70\sl-310\slmult0 \up0 \expndtw-2\charscalex100 Prepare a series of standard sol
utions by pipetting 5 ml, 10 and 20 ml of the solution \par\pard\ql \li1130\ri94
0\sb42\sl-466\slmult0 \up0 \expndtw-4\charscalex100 into 100 ml volumetric flask
s and diluting to mark with water. These standard solutions \line \up0 \expndtw4\charscalex100 contain 2.5 g, 5.0 g, and 10 g sodium nitrite respectively. The sta
ndard solutions \line \up0 \expndtw-5\charscalex100 and the 0.05 gm /1 solution
from which they are prepared shall be made on the day of \line \up0 \expndtw-6\c
harscalex100 the use. \par\pard\ql \li5918\sb0\sl-218\slmult0 \par\pard\ql\li591
8\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\
sb0\sl-218\slmult0 \par\pard\ql\li5918\sb147\sl-218\slmult0 \up0 \expndtw-2\char
scalex100 \ul0\nosupersub\cf10\f11\fs19 5
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}}
{\shp {\*\shpinst\shpleft1104\shptop4837\shpright10828\shpbottom5305\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1120\shptop5903\shpright10828\shpbottom6215\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1111\shptop6836\shpright10828\shpbottom7151\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1816\shptop7151\shpright10828\shpbottom7621\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1816\shptop8094\shpright10828\shpbottom8567\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1816\shptop9505\shpright10828\shpbottom9971\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1106\shptop10448\shpright10828\shpbottom10916\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft1106\shptop10916\shpright10828\shpbottom11382\shpfhdr0\
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{\shp {\*\shpinst\shpleft1106\shptop11382\shpright10828\shpbottom11850\shpfhdr0\
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{\shp {\*\shpinst\shpleft1104\shptop11860\shpright10828\shpbottom12330\shpfhdr0\
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{\shp {\*\shpinst\shpleft1104\shptop12330\shpright10828\shpbottom12796\shpfhdr0\
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{\shp {\*\shpinst\shpleft1104\shptop12796\shpright10828\shpbottom13264\shpfhdr0\
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{\shp {\*\shpinst\shpleft1104\shptop13264\shpright10828\shpbottom13732\shpfhdr0\
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{\shp {\*\shpinst\shpleft1104\shptop13732\shpright10828\shpbottom14197\shpfhdr0\
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{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g8}{\bkmkend Pg8}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex10
0 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUCTS
\par\pard\ql \li1135\sb0\sl-310\slmult0 \par\pard\ql\li1135\sb202\sl-310\slmult
0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf1\f2\fs27 (c) Solution for col
our development \par\pard\li1130\sb0\sl-310\slmult0\par\pard\li1130\sb0\sl-310\s
lmult0\par\pard\li1130\sb26\sl-310\slmult0\fi0\tx7206 \up0 \expndtw0\charscalex1
11 (1)Dissolve by heating on a water bath,\tab \up0 \expndtw0\charsc
alex111 2 gm of sulphanilamide in\par\pard\li1130\sb160\sl-310\slmult0\f
i0\tx8615 \up0 \expndtw0\charscalex111 800 ml water. Cool, filter if
necessary and add\tab \up0 \expndtw0\charscalex111 100 ml of cone\par
\pard\ql \li1130\sb144\sl-310\slmult0 \up0 \expndtw-5\charscalex100 HC1 while st
irring. Dilute to 1000 ml with water. \par\pard\qj \li1130\ri868\sb47\sl-460\slm
ult0 \up0 \expndtw-2\charscalex100 (2) Dissolve 0.25gm of N - napthyl ethylenedi
amine dihydrochloride in water. Dilute to \line \up0 \expndtw-1\charscalex100 25
0 ml with water Store in a stoppered brown bottle in a refrigerator for not more
than \up0 \expndtw-2\charscalex100 one week \par\pard\ql \li1130\sb0\sl-310\slm
ult0 \par\pard\ql\li1130\sb174\sl-310\slmult0 \up0 \expndtw-3\charscalex100 (3)
Dilute 445 ml of Concentrated HCl (sp.gr 1.19) to 1000 ml with water. \par\pard\
ql \li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb200\sl-310\slmult0 \up0 \exp
ndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.2.3 A Apparatus \par\pard\
qj \li1845\ri3129\sb470\sl-480\slmult0\tx2224 \up0 \expndtw-5\charscalex100 \ul0
\nosupersub\cf1\f2\fs27 (1)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2
\fs27 Meat mincer - fitted with a perforated plate with holes not \line\tab \up0
\expndtw-5\charscalex100 greater than 4 mm in diameter. \par\pard\ql \li1845\sb
141\sl-310\slmult0 \up0 \expndtw-4\charscalex100 (2)\ul0\nosupersub\cf15\f16\fs2
7 \ul0\nosupersub\cf1\f2\fs27 Analytical Balance \par\pard\ql \li1845\sb150\sl310\slmult0 \up0 \expndtw-6\charscalex100 (3)\ul0\nosupersub\cf15\f16\fs27 \ul0
\nosupersub\cf1\f2\fs27 Volumetic flasks - 100 ml, 250 and 1000 ml \par\pard\ql
\li1845\sb150\sl-310\slmult0 \up0 \expndtw-5\charscalex100 (4)\ul0\nosupersub\cf
15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Pipette 10 ml \par\pard\ql \li1845\sb17
0\sl-310\slmult0 \up0 \expndtw-5\charscalex100 (5)\ul0\nosupersub\cf15\f16\fs27
\ul0\nosupersub\cf1\f2\fs27 Conical flask \par\pard\ql \li1845\sb150\sl-310\slm
ult0 \up0 \expndtw-5\charscalex100 (6)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupe
rsub\cf1\f2\fs27 Boiling water bath \par\pard\ql \li1845\sb170\sl-310\slmult0 \u
p0 \expndtw-5\charscalex100 (7)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf
1\f2\fs27 Fluted filter paper \par\pard\ql \li1845\sb170\sl-310\slmult0 \up0 \ex
pndtw-4\charscalex100 (8)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\f
s27 Photoelectric colorimeter or spectrophotometer. \par\pard\ql \li1149\sb0\sl310\slmult0 \par\pard\ql\li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb0\sl-31
0\slmult0 \par\pard\ql\li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb290\sl-31
0\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.2.4. A P
rocedure \par\pard\ql \li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\
slmult0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\sl
mult0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\slmu
lt0 \par\pard\ql\li5918\sb18\sl-218\slmult0 \up0 \expndtw-2\charscalex100 \ul0\n
osupersub\cf10\f11\fs19 6
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1104\shptop3880\shpright11071\shpbottom4348\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz105\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1104\shptop4348\shpright10828\shpbottom4813\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft1104\shptop4813\shpright10828\shpbottom5281\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1104\shptop5281\shpright10828\shpbottom5749\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft1104\shptop5749\shpright10828\shpbottom6215\shpfhdr0\sh
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1104\shptop6529\shpright10828\shpbottom7472\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz269\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,943);(9724,943);(9724,0);(0,0)}}
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{\shp {\*\shpinst\shpleft1816\shptop7847\shpright10324\shpbottom8312\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1816\shptop8312\shpright10324\shpbottom8780\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz336\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8508,468);(8508,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1816\shptop8785\shpright10828\shpbottom9253\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz350\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9012,468);(9012,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1816\shptop9253\shpright10828\shpbottom9724\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz384\shplid12
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft1816\shptop9724\shpright10828\shpbottom10192\shpfhdr0\s
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1816\shptop10192\shpright10828\shpbottom10667\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft1816\shptop10667\shpright10828\shpbottom11132\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

\sn geoRight}{\sv 9012}}{\sp{\sn geoBottom}{\sv 465}}

{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9012,465);(9012,0);(0,0)}}
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{\shp {\*\shpinst\shpleft1816\shptop11132\shpright10828\shpbottom11605\shpfhdr0\
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}}
{\shp {\*\shpinst\shpleft1816\shptop11605\shpright10828\shpbottom12076\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz542\shplid17
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{\shp {\*\shpinst\shpleft1120\shptop12676\shpright10828\shpbottom12988\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft1120\shptop12988\shpright10828\shpbottom13900\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g9}{\bkmkend Pg9}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex10

0 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUCTS
\par\pard\ql \li1130\sb0\sl-465\slmult0 \par\pard\ql\li1130\ri861\sb59\sl-465\s
lmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Weigh to the ne
arest 0.00 l gm, about 10 gm of the test sample, transfer quantitatively to \up0
\expndtw-2\charscalex100 a 300 ml conical flask and add successively 5 ml of sa
turated borax solution and 100 ml \up0 \expndtw-3\charscalex100 water at a tempe
rature not below 70 C Heat the flask for 15 minutes on the boiling \line \up0 \ex
pndtw-2\charscalex100 water bath and shake repeatedly. Allow the flask and its c
ontents to cool to room \line \up0 \expndtw-2\charscalex100 temperature and add
successively 2 ml of Pot ferrocyanide followed by 2 ml of zinc \line \up0 \expnd
tw-3\charscalex100 acetate. Mix thoroughly after each addition. Transfer the con
tents to a 200 ml \line \up0 \expndtw-3\charscalex100 volumetric flask. Dilute t
o mark with water and mix. Allow the flask to stand for 30 \line \up0 \expndtw-3
\charscalex100 minutes at room temperature. Carefully decant the supernatant liq
uid and filter it \line \up0 \expndtw-4\charscalex100 through fluted filter pape
r to obtain clear solution. \par\pard\ql \li1130\sb0\sl-466\slmult0 \par\pard\ql
\li1130\ri759\sb9\sl-466\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\c
f18\f19\fs27 Colour Development - Pipette an aliquot of the filtrate ( v ml ) no
t more than 25 ml into \line \up0 \expndtw-4\charscalex100 a 100 ml volumetric f
lask and add water to make upto 60 ml. Add 10 ml of \line \up0 \expndtw-4\charsc
alex100 sulphanilamide solution followed by 6 ml of cone HC1 and leave the solut
ion in the dark \line \up0 \expndtw-3\charscalex100 for 5 minutes. Add 2 ml of N
-Napthylethylenediamine solution and leave for 5- 10 \line \up0 \expndtw-4\char
scalex100 minutes in the dark. Dilute to mark with water. Measure the absorbance
of the solution in \line \up0 \expndtw-4\charscalex100 a 1 cm cell using a phot
oelectric colorimeter or spectrophotometer at a wave length of \line \up0 \expnd
tw-5\charscalex100 about 538 nm \par\pard\qj \li1130\sb0\sl-460\slmult0 \par\par
d\qj\li1130\ri846\sb39\sl-460\slmult0 \up0 \expndtw-2\charscalex100 Prepare a ca
libration curve by taking 10ml water in 4 separate volumetric flasks, adding \up
0 \expndtw0\charscalex105 10 ml each of the standard sodium nitrite solution con
taining 2.5, 5.0 and 10 g of \up0 \expndtw-4\charscalex100 nitrite / ml, developi
ng the colour and measuring as above \par\pard\ql \li1130\sb0\sl-310\slmult0 \pa
r\pard\ql\li1130\sb294\sl-310\slmult0 \up0 \expndtw-5\charscalex100 \ul0\nosuper
sub\cf19\f20\fs27 1.2.5 A\ul0\nosupersub\cf18\f19\fs27 \ul0\nosupersub\cf19\f20
\fs27 Calculation \par\pard\ql \li1130\sb170\sl-310\slmult0 \up0 \expndtw-6\char
scalex100 \ul0\nosupersub\cf18\f19\fs27 Nitrite content expressed as NaNO \ul0\s
ub\cf20\f21\fs26 2\ul0\nosupersub\cf18\f19\fs27 \ul0\super\cf20\f21\fs26 =\ul0\
nosupersub\cf18\f19\fs27 c x 2000 \par\pard\li2301\sb166\sl-310\slmult0\fi3074
\up0 \expndtw-8\charscalex100 M x V\par\pard\li2301\sb163\sl-310\slmult0\fi0 \up
0 \expndtw-8\charscalex100 Where\par\pard\ql \li5918\sb0\sl-218\slmult0 \par\par
d\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\
ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb65\sl-218\slmult0 \up0 \expnd
tw-2\charscalex100 \ul0\nosupersub\cf10\f11\fs19 7
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{\shp {\*\shpinst\shpleft1104\shptop3529\shpright10828\shpbottom3997\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1104\shptop13830\shpright10828\shpbottom14305\shpfhdr0\
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g10}{\bkmkend Pg10}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\s
b0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\qj \li3247\sb0\sl-460\slmult0 \par\pard\qj\li3247\ri1349\sb68\sl-46
0\slmult0 \up0 \expndtw-6\charscalex100 \ul0\nosupersub\cf18\f19\fs27 V = volume
in ml of aliquot portion of filterate taken for test M = \up0 \expndtw-6\charsc
alex100 mass in gm of sample taken \par\pard\ql \li3256\sb144\sl-310\slmult0 \up
0 \expndtw-3\charscalex100 c = concentration of sodium nitrite in ug / ml read f
rom the \par\pard\qj \li3256\ri808\sb27\sl-460\slmult0 \up0 \expndtw-5\charscale
x100 calibration curve that corresponds with the absorbance of the solution \up0
\expndtw-5\charscalex100 prepared from the sample \par\pard\qj \li1130\sb0\sl-4
80\slmult0 \par\pard\qj\li1130\ri995\sb464\sl-480\slmult0 \up0 \expndtw-2\charsc
alex100 ( Ref:- I.S 5960 ( Part VII): 1996 / I.S.O 2918 : 1975 Meat and Meat Pro
ducts Methods \up0 \expndtw-4\charscalex100 of Test - Determination of Nitrite c
ontent) \par\pard\ql \li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb0\sl-310\s
lmult0 \par\pard\ql\li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb131\sl-310\s
lmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.3 Determina
tion of Ascorbic acid ( Rule 72): \par\pard\ql \li1130\sb0\sl-310\slmult0 \par\p
ard\ql\li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb270\sl-310\slmult0 \up0 \
expndtw-7\charscalex100 1.3.1Principle:-\par\pard\ql \li1130\sb0\sl-465\slmult0
\par\pard\ql\li1130\ri915\sb37\sl-465\slmult0 \up0 \expndtw-3\charscalex100 \ul0
\nosupersub\cf1\f2\fs27 Ascorbic acid reduces oxidation-reduction indicator dye
2, 6 dichlorophenol indophenol \line \up0 \expndtw-4\charscalex100 to colorless
solution. At end point excess unreacted dye is rose pink in acid solution. \line
\up0 \expndtw-4\charscalex100 Vitamin is extracted and titration performed in p
resence of metaphosphoric acid-acetic \line \up0 \expndtw-2\charscalex100 acid s
olution to maintain proper acidity and avoid auto oxidation of ascorbic acid at
\line \up0 \expndtw-4\charscalex100 high pH \par\pard\ql \li1192\sb0\sl-310\slmu
lt0 \par\pard\ql\li1192\sb0\sl-310\slmult0 \par\pard\ql\li1192\sb4\sl-310\slmult
0 \up0 \expndtw-6\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.3.2 Reagents:-\p
ar\pard\ql \li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb0\sl-310\slmult0 \pa
r\pard\ql\li1130\sb30\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupers
ub\cf1\f2\fs27 (a) Extracting solution -\par\pard\qj \li1130\ri1017\sb47\sl-460\
slmult0 \up0 \expndtw-4\charscalex100 Metaphosphoric acid-acetic acid solution-D
issolve with shaking 15gm HPO\ul0\sub\cf17\f18\fs26 3\ul0\nosupersub\cf1\f2\fs27
pellets or \line \up0 \expndtw-4\charscalex100 freshly pulverized sticks in 40
ml acetic acid and 200ml water. Dilute to 500ml. Filter \par\pard\ql \li5918\sb0
\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb164
\sl-218\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf10\f11\fs19 8
{\shp {\*\shpinst\shpleft3218\shptop2128\shpright10828\shpbottom2600\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 7610}}{\sp{\sn geoBottom}{\sv 472}}
{\sp{\sn pVerticies}{\sv 8;4;(0,472);(7610,472);(7610,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft3218\shptop2600\shpright10828\shpbottom3071\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz53\shplid1
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 7610}}{\sp{\sn geoBottom}{\sv 471}}
{\sp{\sn pVerticies}{\sv 8;4;(0,471);(7610,471);(7610,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft3228\shptop3071\shpright10828\shpbottom3539\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz70\shplid2
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 7600}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(7600,468);(7600,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft3228\shptop3539\shpright10828\shpbottom4007\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz81\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 7600}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(7600,468);(7600,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft3228\shptop4007\shpright10828\shpbottom4472\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz95\shplid4
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 7600}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(7600,465);(7600,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop5413\shpright10828\shpbottom5884\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz116\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 471}}
{\sp{\sn pVerticies}{\sv 8;4;(0,471);(9724,471);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1104\shptop5884\shpright10828\shpbottom6356\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz139\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 472}}
{\sp{\sn pVerticies}{\sv 8;4;(0,472);(9724,472);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop6356\shpright10828\shpbottom7770\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz164\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 1414}}
{\sp{\sn pVerticies}{\sv 8;4;(0,1414);(9724,1414);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop7770\shpright10828\shpbottom7991\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz188\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9717}}{\sp{\sn geoBottom}{\sv 221}}
{\sp{\sn pVerticies}{\sv 8;4;(0,221);(9717,221);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop8622\shpright10828\shpbottom8936\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz202\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9724,314);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop8936\shpright10828\shpbottom9899\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz227\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 963}}
{\sp{\sn pVerticies}{\sv 8;4;(0,963);(9724,963);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1104\shptop9899\shpright10828\shpbottom10367\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz250\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop10367\shpright10828\shpbottom10835\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz264\shplid12
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop10835\shpright10828\shpbottom11300\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz282\shplid13
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9724,465);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop11300\shpright10828\shpbottom11768\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz304\shplid14
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop11768\shpright10828\shpbottom12731\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz319\shplid15
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 963}}
{\sp{\sn pVerticies}{\sv 8;4;(0,963);(9724,963);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1104\shptop12731\shpright10828\shpbottom13664\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz352\shplid16
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 933}}
{\sp{\sn pVerticies}{\sv 8;4;(0,933);(9724,933);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop13664\shpright10828\shpbottom14166\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz380\shplid17
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 502}}
{\sp{\sn pVerticies}{\sv 8;4;(0,502);(9724,502);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop14166\shpright10828\shpbottom14632\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz420\shplid18
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9724,466);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g11}{\bkmkend Pg11}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\s
b0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\qj \li1130\sb0\sl-460\slmult0 \par\pard\qj\li1130\ri1156\sb68\sl-46
0\slmult0 \up0 \expndtw-1\charscalex100 \ul0\nosupersub\cf1\f2\fs27 rapidly thro
ugh fluted filter paper into a glass stoppered bottle. Store in a refrigerator \
up0 \expndtw-1\charscalex100 to prevent change to H\ul0\sub\cf17\f18\fs26 3\ul0\
nosupersub\cf1\f2\fs27 PO\ul0\sub\cf17\f18\fs26 4\ul0\nosupersub\cf1\f2\fs27 . S
olution remains satisfactory for 7-10 days. \par\pard\ql \li1130\sb0\sl-466\slmu
lt0 \par\pard\ql\li1130\ri2520\sb10\sl-466\slmult0 \up0 \expndtw-4\charscalex100
(b) Ascorbic acid standard solution (1 mg/ ml) - Accurately weigh 50mg \up0 \ex
pndtw-3\charscalex100 USP Ascorbic acid reference standard that has been stored
in a dessicator \up0 \expndtw-5\charscalex100 away from sunlight. Transfer to 50
ml vol flask. Dilute to vol with \line \up0 \expndtw-5\charscalex100 metaphospho
ric- acetic acid extracting solution before use. \par\pard\qj \li1130\ri1406\sb4
69\sl-473\slmult0 \up0 \expndtw-3\charscalex100 (c ) Indophenol standard solutio
n:-Dissolve 50mg 2 ,6 dichlorophenol indophenol \line \up0 \expndtw-3\charscalex
100 sodium salt in 50ml of water to which have been added 42 mg of NaHCO\ul0\sub
\cf17\f18\fs26 3\ul0\nosupersub\cf1\f2\fs27 . Shake \line \up0 \expndtw-3\charsc
alex100 vigorously and when dye dissolves dilute to 200ml with water. Filter thr
ough fluted \line \up0 \expndtw-3\charscalex100 filter in an amber colored glass
bottle. Keep stoppered and store in a refrigerator. \par\pard\ql \li1130\sb0\sl

-466\slmult0 \par\pard\ql\li1130\ri1018\sb27\sl-466\slmult0 \up0 \expndtw-3\char

scalex100 (Note:-Decomposition products that make end point indistinct occur in
some batches of \up0 \expndtw-3\charscalex100 dry indophenol and also devolop wi
th time in stock solution. Add 5ml of extracting \up0 \expndtw-3\charscalex100 s
olution with excess ascorbic acid to 15ml of dye solution. If reduced solution i
s not \up0 \expndtw-4\charscalex100 practically colorless discard and prepare ne
w stock solution.) \par\pard\ql \li1130\sb0\sl-465\slmult0 \par\pard\ql\li1130\r
i1053\sb10\sl-465\slmult0 \up0 \expndtw-2\charscalex100 Standardisation of Indop
henol solution - Transfer 3 aliquots of 2.0 ml Ascorbic acid \line \up0 \expndtw
-4\charscalex100 standard sol to 3 conical flasks containing 5ml of metaphosphor
ic- acetic acid \line \up0 \expndtw-4\charscalex100 extracting solution. Titrate
rapidly with indophenol dye from 50ml burette until a light \line \up0 \expndtw
-3\charscalex100 distinct rose pink remains for 5 seconds. Each titration should
require about 15ml \line \up0 \expndtw-3\charscalex100 indophenol solution and
differ from each other by 0.1 ml. Similarly titrate 3 blanks \line \up0 \expndtw
-3\charscalex100 composed of 7ml of HPO\ul0\sub\cf17\f18\fs26 3\ul0\nosupersub\c
f1\f2\fs27 - CH\ul0\sub\cf17\f18\fs26 3\ul0\nosupersub\cf1\f2\fs27 COOH + wate
r equal to the vol of indolphenol sol \line \up0 \expndtw-3\charscalex100 used i
n earlier titration. Titrate with indolphenol. Titre for blank should be approx
0.1 \line \up0 \expndtw-4\charscalex100 ml. Substract blank from earlier titrati
on and calculate concentration of indophenol \par\pard\ql \li5918\sb0\sl-218\slm
ult0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb0\sl-218\slmul
t0 \par\pard\ql\li5918\sb0\sl-218\slmult0 \par\pard\ql\li5918\sb28\sl-218\slmult
0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf10\f11\fs19 9
{\shp {\*\shpinst\shpleft1104\shptop2128\shpright10828\shpbottom2596\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop2596\shpright10828\shpbottom3061\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz53\shplid1
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9724,465);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop3061\shpright10828\shpbottom4007\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz97\shplid2
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 946}}
{\sp{\sn pVerticies}{\sv 8;4;(0,946);(9724,946);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1104\shptop4007\shpright10828\shpbottom4472\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz128\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9724,465);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop4472\shpright10828\shpbottom4940\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz146\shplid4
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop4940\shpright10828\shpbottom5408\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz160\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop5408\shpright10828\shpbottom6352\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz185\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 944}}
{\sp{\sn pVerticies}{\sv 8;4;(0,944);(9724,944);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop6352\shpright10828\shpbottom6822\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz216\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 470}}
{\sp{\sn pVerticies}{\sv 8;4;(0,470);(9724,470);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1104\shptop6822\shpright10828\shpbottom7297\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz250\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 475}}
{\sp{\sn pVerticies}{\sv 8;4;(0,475);(9724,475);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop7297\shpright10828\shpbottom7770\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz260\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 473}}
{\sp{\sn pVerticies}{\sv 8;4;(0,473);(9724,473);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop8250\shpright10797\shpbottom8716\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz278\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9693}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9693,466);(9693,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop8716\shpright10797\shpbottom9181\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz299\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9693}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9693,465);(9693,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop9181\shpright10797\shpbottom9649\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz313\shplid12
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9693}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9693,468);(9693,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1104\shptop9649\shpright10797\shpbottom10117\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz331\shplid13
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9693}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9693,468);(9693,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop10588\shpright10828\shpbottom11056\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz350\shplid14
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop11056\shpright10828\shpbottom11524\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz381\shplid15
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop11524\shpright10828\shpbottom11989\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz399\shplid16
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9724,465);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop11989\shpright10828\shpbottom12457\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz421\shplid17
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1104\shptop12457\shpright10828\shpbottom12925\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz431\shplid18
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop12925\shpright10828\shpbottom13391\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz449\shplid19
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9724,466);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop13391\shpright10828\shpbottom13859\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz491\shplid20
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop13859\shpright10828\shpbottom14327\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz509\shplid21
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g12}{\bkmkend Pg12}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\s
b0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb0\sl-310\slmult
0 \par\pard\ql\li1130\sb32\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nos
upersub\cf1\f2\fs27 solution as mg ascorbic acid equivalent to 1 ml of solution.
\par\pard\qj \li1144\sb0\sl-460\slmult0 \par\pard\qj\li1144\ri1540\sb47\sl-460\
slmult0 \up0 \expndtw-4\charscalex100 Standardise indophenol solution daily with
freshly prepared ascorbic acid standard \up0 \expndtw-5\charscalex100 solution.
\par\pard\ql \li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb14\sl-310\slmult0

\up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.3.3 Procedure \pa

r\pard\ql \li1130\sb0\sl-466\slmult0 \par\pard\ql\li1130\ri930\sb36\sl-466\slmul
t0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Take about 25-50 gm
of prepared sample (containing 100 to 200mg ascorbic acid). \line \up0 \expndtw
-2\charscalex100 Dilute with 100ml of metaphosphoric-acetic acid solution and mi
x thoroughly in a \up0 \expndtw-2\charscalex100 laboratory homogeniser. Centrifu
ge and decant the supernatant liquid through an acid \up0 \expndtw-2\charscalex1
00 washed filter paper (Whatman 541).Take 3 sample aliquots containing about 2.0
mg of \up0 \expndtw-2\charscalex100 ascorbic acid, add 5ml of metaphosphoric-ace
tic acid solution. Make a blank using 7ml \up0 \expndtw-2\charscalex100 of extra
cting solution Titrate with indophenol solution Titrate blank also by diluting i
t \up0 \expndtw-3\charscalex100 with water to the extent of the indophenol sol u
sed in the sample. \par\pard\ql \li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\s
b293\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27
1.3.4 Calculation \par\pard\ql \li1195\sb0\sl-310\slmult0 \par\pard\ql\li1195\sb
0\sl-310\slmult0 \par\pard\ql\li1195\sb10\sl-310\slmult0 \up0 \expndtw-3\charsca
lex100 \ul0\nosupersub\cf1\f2\fs27 Ascorbic acid mg/100 gm = \par\pard\ql \li221
0\sb0\sl-310\slmult0 \par\pard\ql\li2210\sb0\sl-310\slmult0 \par\pard\ql\li2210\
sb10\sl-310\slmult0 \up0 \expndtw-4\charscalex100 Sample titre - blank x mg asco
rbic acid/ml x Vol made x l00 \par\pard\ql \li3650\sb170\sl-310\slmult0 \up0 \ex
pndtw-4\charscalex100 Aliquot taken x wt of sample \par\pard\qj \li1130\sb0\sl-4
80\slmult0 \par\pard\qj\li1130\sb0\sl-480\slmult0 \par\pard\qj\li1130\ri785\sb47
0\sl-480\slmult0 \up0 \expndtw-4\charscalex100 (Reference:- A.O.A.C 17\ul0\super
\cf17\f18\fs26 th\ul0\nosupersub\cf1\f2\fs27 edition,2000, Official method 967.
21 Ascorbic acid in vitamin \up0 \expndtw-6\charscalex100 preparation and juices
) \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0
\par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \
par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb67\sl-218\slmult0 \u
p0 \expndtw-2\charscalex100 \ul0\nosupersub\cf10\f11\fs19 10
{\shp {\*\shpinst\shpleft1104\shptop2128\shpright10828\shpbottom2596\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1116\shptop3076\shpright10828\shpbottom3544\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz58\shplid1
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9712}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9712,468);(9712,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1116\shptop3544\shpright10828\shpbottom4011\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz76\shplid2
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9712}}{\sp{\sn geoBottom}{\sv 467}}
{\sp{\sn pVerticies}{\sv 8;4;(0,467);(9712,467);(9712,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}

}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1120\shptop4011\shpright10828\shpbottom4324\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz90\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9708}}{\sp{\sn geoBottom}{\sv 313}}
{\sp{\sn pVerticies}{\sv 8;4;(0,313);(9708,313);(9708,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1120\shptop4324\shpright10828\shpbottom4636\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz99\shplid4
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9708}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9708,312);(9708,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop5137\shpright10828\shpbottom5603\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz125\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9724,466);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop5603\shpright10828\shpbottom6071\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz148\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop6071\shpright10828\shpbottom6539\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz169\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}

}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop6539\shpright10828\shpbottom7004\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz183\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9724,465);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop7004\shpright10828\shpbottom7472\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz197\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop7472\shpright10828\shpbottom7940\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz226\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop7940\shpright10828\shpbottom8406\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz244\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9724,466);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1120\shptop8500\shpright10828\shpbottom9440\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz263\shplid12
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9708}}{\sp{\sn geoBottom}{\sv 940}}
{\sp{\sn pVerticies}{\sv 8;4;(0,940);(9708,940);(9708,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}

}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1168\shptop9440\shpright10828\shpbottom10379\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz290\shplid13
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9660}}{\sp{\sn geoBottom}{\sv 939}}
{\sp{\sn pVerticies}{\sv 8;4;(0,939);(9660,939);(9660,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2181\shptop10379\shpright10828\shpbottom11320\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz306\shplid14
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8647}}{\sp{\sn geoBottom}{\sv 941}}
{\sp{\sn pVerticies}{\sv 8;4;(0,941);(8647,941);(8647,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2210\shptop11161\shpright8812\shpbottom11181\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz339\shplid15
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 6602}}{\sp{\sn geoBottom}{\sv 20}}
{\sp{\sn pVerticies}{\sv 8;4;(0,20);(6602,20);(6602,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 0}}{\s
p{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv 1}}{\s
p{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop11353\shpright10828\shpbottom11665\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz349\shplid16
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9724,312);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop11665\shpright10828\shpbottom12628\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz368\shplid17
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 963}}
{\sp{\sn pVerticies}{\sv 8;4;(0,963);(9724,963);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}

}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop12628\shpright10828\shpbottom13590\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz378\shplid18
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 962}}
{\sp{\sn pVerticies}{\sv 8;4;(0,962);(9724,962);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop13590\shpright10828\shpbottom14058\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz417\shplid19
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g13}{\bkmkend Pg13}\par\pard\li1144\sb0\sl-184\slmult0\par\pard\li1144\sb0\sl-18
4\slmult0\par\pard\li1144\sb0\sl-184\slmult0\par\pard\li1144\sb0\sl-184\slmult0\
par\pard\li1144\sb0\sl-184\slmult0\par\pard\li1144\sb0\sl-184\slmult0\par\pard\l
i1144\sb95\sl-184\slmult0\fi5085 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\c
f9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUCTS\par\pard\li1144\sb
0\sl-310\slmult0\par\pard\li1144\sb206\sl-310\slmult0\fi0 \up0 \expndtw-4\charsc
alex100 \ul0\nosupersub\cf14\f15\fs27 1. 3. A \ul0\nosupersub\cf1\f2\fs27 - \ul0
\nosupersub\cf14\f15\fs27 Alternate method for Ascorbic acid\par\pard\ql \li1500
\sb0\sl-470\slmult0 \par\pard\ql\li1500\ri847\sb13\sl-470\slmult0 \up0 \expndtw2\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Extract 50 gm prepared sample with 1
00 ml meta phosphoric acid - acetic acid \line \up0 \expndtw-3\charscalex100 mix
ture prepared by dissolving 30 gm metaphosphoric acid in 1000 ml water \line \up
0 \expndtw-3\charscalex100 containing 80 ml glacial acetic acid. Mix thoroughly
in a laboratory \line \up0 \expndtw-3\charscalex100 homogenizer. Centrifuge and
decant the supernatant through an acid washed filter \up0 \expndtw-1\charscalex1
00 paper( Whatman 541). Titrate 2 ml of the extract with a solution of 2 , 6 - d
ichloro -\line \up0 \expndtw0\charscalex100 N - p -hydroxy phenyl- p - benzoquin
one monoamine (30 gm in 200 ml water) \up0 \expndtw-1\charscalex100 until a perm
anent pink colour persists for at least 1 minute. \par\pard\ql \li1860\sb123\sl310\slmult0 \up0 \expndtw-4\charscalex100 0.1 ml = 7 mg of total ascorbate \par\
pard\ql \li1504\sb0\sl-310\slmult0 \par\pard\ql\li1504\sb0\sl-310\slmult0 \par\p
ard\ql\li1504\sb10\sl-310\slmult0 \up0 \expndtw-3\charscalex100 (Ref:- Pearsons
Composition and Analysis of Foods 9\ul0\super\cf17\f18\fs26 th\ul0\nosupersub\cf
1\f2\fs27 edn1991 , page 500 ) \par\pard\ql \li1154\sb0\sl-310\slmult0 \par\par
d\ql\li1154\sb0\sl-310\slmult0 \par\pard\ql\li1154\sb0\sl-310\slmult0 \par\pard\
ql\li1154\sb180\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf1
4\f15\fs27 1.4 Determination of Total Phosphorous content ( rule 72) :-\par\pard
\ql \li1154\sb0\sl-310\slmult0 \par\pard\ql\li1154\sb0\sl-310\slmult0 \par\pard\
ql\li1154\sb10\sl-310\slmult0 \up0 \expndtw-4\charscalex100 1.4.1 Principle \par
\pard\ql \li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb0\sl-310\slmult0 \par\
pard\ql\li1130\sb10\sl-310\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub

\cf1\f2\fs27 Mineralization of a test portion (wet digestion) with sulphuric and

nitric acid, \par\pard\ql \li1130\ri932\sb22\sl-466\slmult0 \up0 \expndtw-1\cha
rscalex100 precipitation of phosphorous as quinoline phosphomolybdate and drying
and weighing \up0 \expndtw-3\charscalex100 of the precipitate. Alternatively th
e sample can be ashed and ash taken up in 15 ml cone \up0 \expndtw-2\charscalex1
00 nitric acid in a conical flask adding water to make upto 75 ml, heating on a
boiling \line \up0 \expndtw-3\charscalex100 water bath for 30 minutes , cooling
and making upto a known volume. \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\par
d\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\
ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql
\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\l
i5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5
867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li586
7\sb41\sl-218\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf10\f11\fs1
9 11
{\shp {\*\shpinst\shpleft1116\shptop2128\shpright10828\shpbottom2440\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
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{\shp {\*\shpinst\shpleft1471\shptop2936\shpright10828\shpbottom3407\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1471\shptop3407\shpright10828\shpbottom3880\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1471\shptop3880\shpright10828\shpbottom4352\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1831\shptop6224\shpright10828\shpbottom6695\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft1476\shptop7292\shpright10828\shpbottom7604\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1125\shptop8704\shpright10828\shpbottom9016\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1125\shptop9016\shpright10828\shpbottom9947\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1104\shptop10446\shpright10828\shpbottom10914\shpfhdr0\
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{\shp {\*\shpinst\shpleft1104\shptop10914\shpright10828\shpbottom11380\shpfhdr0\
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{\shp {\*\shpinst\shpleft1125\shptop13852\shpright10828\shpbottom14166\shpfhdr0\
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g14}{\bkmkend Pg14}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\s
b0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li1154\sb0\sl-310\slmult0 \par\pard\ql\li1154\sb222\sl-310\slmu
lt0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.4.2 Reagents \
par\pard\ql \li1135\sb0\sl-310\slmult0 \par\pard\ql\li1135\sb300\sl-310\slmult0
\up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf1\f2\fs27 (1)\ul0\nosupersub\cf1

5\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Cone. Sulphuric acid - 1.84 gm / ml \par

\pard\ql \li1135\sb150\sl-310\slmult0 \up0 \expndtw-2\charscalex100 (2)\ul0\nosu
persub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Cone. Nitric acid - 1.40 gm /
ml \par\pard\qj \li1130\ri882\sb0\sl-468\slmult0 \up0 \expndtw0\charscalex103 (3
) Precipitating reagent - Dissolve 70 gm of Sod. Molybdate dehydrate in 150 m
l \up0 \expndtw0\charscalex103 water. Dissolve 60 gm of Citric acid monohydrate
in 150 ml water and add 85 ml of \up0 \expndtw-1\charscalex100 cone nitric acid.
Mix the two solutions and stir slowly. To another 100 ml water add 25 \up0 \exp
ndtw0\charscalex104 ml nitric acid and 5 ml of distilled quinoline. Gradually ad
d this solution to the first \up0 \expndtw0\charscalex104 solution while stirrin
g. Leave for 24 hrs at room temperature. Store the reagent in a \up0 \expndtw-3\
charscalex100 stoppered plastic bottle in the dark \par\pard\ql \li1149\sb0\sl-3
10\slmult0 \par\pard\ql\li1149\sb293\sl-310\slmult0 \up0 \expndtw-4\charscalex10
0 \ul0\nosupersub\cf14\f15\fs27 1.4.3 Apparatus \par\pard\qj \li1500\sb0\sl-460\
slmult0 \par\pard\qj\li1500\ri2633\sb47\sl-460\slmult0\tx1879 \up0 \expndtw-4\ch
arscalex100 \ul0\nosupersub\cf1\f2\fs27 (1) Mechanical meat mincer - fitted with
a plate with holes of dia not \line\tab \up0 \expndtw-5\charscalex100 exceeding
4 mm. \par\pard\ql \li1500\sb144\sl-310\slmult0 \up0 \expndtw-3\charscalex100 (
2) Analytical balance \par\pard\ql \li1495\sb150\sl-310\slmult0 \up0 \expndtw-4
\charscalex100 (3)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Kje
ldahl flask \par\pard\ql \li1495\ri2008\sb47\sl-460\slmult0\tx1874\tx1874 \up0 \
expndtw-3\charscalex100 (4)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2
\fs27 Heating device on which the flask can be heated in an inclined position \l
ine\tab \up0 \expndtw-2\charscalex100 in such a way that the source of heat only
touches the wall of the flask \line \tab \up0 \expndtw-3\charscalex100 which is
below the level of the liquid. \par\pard\ql \li1495\sb144\sl-310\slmult0 \up0 \
expndtw-3\charscalex100 (5)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2
\fs27 Suction device to remove the acid fumes formed during the digestion. \par\
pard\ql \li1495\sb150\sl-310\slmult0 \up0 \expndtw-2\charscalex100 (6)\ul0\nosup
ersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Fritted glass filter - pore dia
meter 5 -1 5 mm. \par\pard\ql \li1495\sb170\sl-310\slmult0 \up0 \expndtw-5\chars
calex100 (7)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Drying ov
en capable of being adjusted to 260 \u8212? 20 C \par\pard\ql \li1495\sb150\sl-3
10\slmult0 \up0 \expndtw-4\charscalex100 (8)\ul0\nosupersub\cf15\f16\fs27 \ul0\
nosupersub\cf1\f2\fs27 Conical suction flask \par\pard\ql \li1495\sb170\sl-310\s
lmult0 \up0 \expndtw-4\charscalex100 (9)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosu
persub\cf1\f2\fs27 Dessicator \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\
ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql
\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\l
i5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5
867\sb40\sl-218\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf10\f11\f
s19 12
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45140051451401ffd9}}}}}\pard\sb0\sl-240{\bkmkstart Pg15}{\bkmkend Pg15}\par\pard
\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\
ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql
\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\l
i6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf9\f10\
fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUCTS \par\pard\ql \li1149\sb0\
sl-310\slmult0 \par\pard\ql\li1149\sb202\sl-310\slmult0 \up0 \expndtw-4\charscal
ex100 \ul0\nosupersub\cf14\f15\fs27 1.4.4 Procedure \par\pard\ql \li1135\sb0\sl470\slmult0 \par\pard\ql\li1135\ri1013\sb28\sl-470\slmult0\fi9 \up0 \expndtw0\ch
arscalex102 \ul0\nosupersub\cf1\f2\fs27 Make the sample homogeneous by passing i
t at least twice through the meat mincer \up0 \expndtw0\charscalex101 and mixing
. Keep the homogenisd sample in a completely filled \line \up0 \expndtw-3\charsc
alex100 airtight closed container and store it in such a way that deterioration
and change in \line \up0 \expndtw-3\charscalex100 composition is prevented. Anal
yse the sample as soon as possible, but in any case \line \up0 \expndtw0\charsca
lex100 within 24 hrs. If the sample is not immediately analysed after passage th
rough the \line \up0 \expndtw-1\charscalex100 mincer, liquid separation may occu
r. Therefore homogenize the sample thoroughly \line \up0 \expndtw-4\charscalex10
0 immediately before testing. \par\pard\ql \li1130\sb0\sl-310\slmult0 \par\pard\
ql\li1130\sb293\sl-310\slmult0 \up0 \expndtw-2\charscalex100 Weigh to the neares
t 0.001 gm about 3 gm of sample into the flask , add 20 ml nitric \par\pard\ql \
li1130\ri881\sb20\sl-468\slmult0 \up0 \expndtw-2\charscalex100 acid and some gla
ss beads. Place the flask in an inclined position on the heating device \line \u
p0 \expndtw-3\charscalex100 and heat for 5 minutes. Cool and then add 5 ml of su
lphuric acid. Heat the flask gently \line \up0 \expndtw-2\charscalex100 until th
e foaming has ceased, then heat more strongly. Add more nitric acid and \line \u
p0 \expndtw-1\charscalex100 continue heating. Repeat the operation until evoluti
on of brown fumes has ceased. \line \up0 \expndtw0\charscalex100 Finally when th
e liquid has become colourless heat until white fumes appear. Cool \line \up0 \e
xpndtw-1\charscalex100 add 15 ml water and boil gently. Transfer the liquid to a
250 ml beaker or conical flask \line \up0 \expndtw-2\charscalex100 rinsing the
flask with water. Add 10 ml nitric acid. The total volume should then be \line \
up0 \expndtw-3\charscalex100 50ml. \par\pard\ql \li1149\sb0\sl-310\slmult0 \par\
pard\ql\li1149\sb293\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersu
b\cf14\f15\fs27 1.4.4.1 Determination \par\pard\qj \li1130\ri1134\sb358\sl-470\s
lmult0 \up0 \expndtw1\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Add 50 ml of the
precipitating reagent to the liquid in the beaker or conical flask. \line \up0
\expndtw1\charscalex100 Cover with watch glass and boil for 1 minute on a hot pl
ate. Allow to cool to room \line \up0 \expndtw-1\charscalex100 temperature, duri
ng cooling swirl the contents three or four times. Filter under the \line \up0 \
expndtw0\charscalex104 suction through the fritted glass filter which has been p
reviously heated for 30 \line \up0 \expndtw-5\charscalex100 minutes at a tempera
ture of 260 \up0 \expndtw-4\charscalex100 20 C, cooled in a dessicator and weigh

ed to the \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218

\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb45\sl-218\
slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf10\f11\fs19 13 \par\pard
\sect\sectd\fs24\paperw11900\paperh16840{\shp
{\*\shpinst\shpleft0\shptop0\shpright11900\shpbottom16840\shpwr3\shpfblwtxt0\shp
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0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a

0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a
0028a28a0028a28a00f9fbf6a5fd98be11fed91f02fc6ffb397c75d33c41acfc2df886ba0af89f4d
f0bf8cfc57e00d6ae8786fc4ba3f8b74a5b6f14782f57d0fc43631a6b5a169d2ddc16ba8c76fa8da
24fa76a10dcd85d5cdbcbf8e5ff10c17fc1227fe895fc70ffc4adfda2bff009e257f41d45007f3e3
ff0010c17fc1227fe895fc70ff00c4adfda2bff9e255ab1ff83637fe0921a7ded9dfdafc2ef8e71d
cd8dd5bde5bc91fed65fb48412473db4a9344e935b7c4982e61759115965b79a19e3203c52c72057
1fd0251400514514005145140051451400514514005145140051451401f87bf19ffe0dddff00825f
fc7cf8b7f12fe377c49f871f19752f883f16bc71e26f889e35bfd3ff0069df8ffa3e9d79e28f176a
f75ae6b771a768ba7fc40874cd134e9350bdb86b0d1349b6b4d1f47b430e9ba4595969b6b6b690f9
7a7fc1b77fb127c2fd5bc33f133f63bf1c7ed01fb287ed19f0ff00c51a1f8afe1c7c73d33e2cf8d7
e365c7846ff4bbc47d42193e197c6cf1078bfe18f8a6cf5dd21eff0042bfb1f15f86f57b0fb1ea13
b9b39245515fd0951401f08fecadfb11eaff00b397880f8efc63fb5ffed85fb4b78ff55f0c6a5a27
8ba5f8dff19f5ad6fe17eadaceb3ab69facea3e2bf0e7c13b5587e1ffc3dd4d2e6c0dae8b65e10b2
b0d27c39a3df6a1a2e8b6769a65c2db45f7751450014514500145145001451450014514500145145
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001451450014514500145145007fffd9}}}}}\pard\sb0\sl-240{\bkmkstart Pg16}{\bkmkend
Pg16}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmu
lt0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult
0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0
\par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosup
ersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUCTS \par\pard\q
l \li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb202\sl-310\slmult0 \up0 \expn
dtw-1\charscalex100 \ul0\nosupersub\cf1\f2\fs27 nearest 1 mg. Wash the ppt on th
e filter five times with 25 ml water using the same \par\pard\ql \li1130\ri832\s
b42\sl-466\slmult0 \up0 \expndtw-1\charscalex100 water to wash away any remainin
g ppt from the conical flask onto the filter. Dry in the \up0 \expndtw-3\charsca
lex100 oven at 260 \up0 \expndtw-3\charscalex100 20 \ul0\super\cf17\f18\fs26 0\u
l0\nosupersub\cf1\f2\fs27 C for 1 hr. Cool in a dessicator and weigh to the nea
rest 1 mg. \line \up0 \expndtw-4\charscalex100 Carry out a blank test using same
procedure and same quantity of reagents but omitting \line \up0 \expndtw-5\char
scalex100 the test portion \par\pard\ql \li1154\sb0\sl-310\slmult0 \par\pard\ql\
li1154\sb0\sl-310\slmult0 \par\pard\ql\li1154\sb23\sl-310\slmult0 \up0 \expndtw5\charscalex100 \ul0\nosupersub\cf14\f15\fs27 Calculation \par\pard\ql \li1135\s
b0\sl-310\slmult0 \par\pard\ql\li1135\sb0\sl-310\slmult0 \par\pard\ql\li1135\sb5
0\sl-310\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Phosp
horous (%) as P\ul0\sub\cf17\f18\fs26 2\ul0\nosupersub\cf1\f2\fs27 O\ul0\sub\cf1
7\f18\fs26 5\ul0\nosupersub\cf1\f2\fs27 = 0.03207 x m, x 100
or = 3.207 x m\
ul0\sub\cf17\f18\fs26 \ul0\nosupersub\cf21\f22\fs13 1 \par\pard\li6144\sb137\sl
-310\slmult0\fi0\tx8124 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf1\f2\fs2
7 m\ul0\sub\cf17\f18\fs26 0\tab \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf
1\f2\fs27 m\ul0\sub\cf17\f18\fs26 0\par\pard\ql \li1144\sb0\sl-310\slmult0 \par\
pard\ql\li1144\sb293\sl-310\slmult0 \up0 \expndtw-5\charscalex100 \ul0\nosupersu
b\cf1\f2\fs27 Where \par\pard\ql \li2551\sb170\sl-310\slmult0 \up0 \expndtw-3\ch
arscalex100 m \ul0\sub\cf17\f18\fs26 0\ul0\nosupersub\cf1\f2\fs27 = mass in gm
of the test portion \par\pard\ql \li2587\sb150\sl-310\slmult0 \up0 \expndtw-6\ch
arscalex100 m \ul0\sub\cf17\f18\fs26 1\ul0\nosupersub\cf1\f2\fs27 = mass in gm
of the quinoline phosphomolybdate precipitate. \par\pard\ql \li1135\sb0\sl-310\s
lmult0 \par\pard\ql\li1135\sb0\sl-310\slmult0 \par\pard\ql\li1135\sb10\sl-310\sl

mult0 \up0 \expndtw-2\charscalex100 Report the result to the nearest 0.01 gm of

phosphorous pentoxide / 100 gm \par\pard\qj \li1130\sb0\sl-460\slmult0 \par\pard
\qj\li1130\sb0\sl-460\slmult0 \par\pard\qj\li1130\sb0\sl-460\slmult0 \par\pard\q
j\li1130\ri1030\sb107\sl-460\slmult0 \up0 \expndtw-2\charscalex100 ( Ref:- I.S 5
960 ( Part 9 ) : 1988 / I.S.O 2294 : 1974 Meat and Meat Products-Methods \up0 \e
xpndtw-3\charscalex100 of Test - Determination of Total Phosphorous Content) \pa
r\pard\ql \li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb294\sl-310\slmult0 \u
p0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.5 Test for presence
of Poly phosphates:-\par\pard\ql \li1154\sb0\sl-310\slmult0 \par\pard\ql\li1154\
sb0\sl-310\slmult0 \par\pard\ql\li1154\sb10\sl-310\slmult0 \up0 \expndtw-4\chars
calex100 1.5.1 Principle \par\pard\ql \li1135\sb0\sl-310\slmult0 \par\pard\ql\li
1135\sb0\sl-310\slmult0 \par\pard\ql\li1135\sb10\sl-310\slmult0 \up0 \expndtw-3\
charscalex100 \ul0\nosupersub\cf1\f2\fs27 Extraction of meat or meat product wit
h trichloro acetic acid, cleaning of the serum \par\pard\ql \li5867\sb0\sl-218\s
lmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb70\sl-218\sl
mult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf10\f11\fs19 14 \par\pard\s
ect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart Pg17}{\bkmkend
Pg17}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slm
ult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmul
t0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0
\par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosu
persub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUCTS \par\pard\
qj \li1135\sb0\sl-460\slmult0 \par\pard\qj\li1135\ri986\sb68\sl-460\slmult0 \up0
\expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 obtained with ethanol / di
ethyl ether mixture, separation of the phosphates by thin layer \up0 \expndtw-3\
charscalex100 chromatography and detection of polyphosphates by spraying with re
agents for colour \up0 \expndtw-4\charscalex100 development. \par\pard\ql \li115
4\sb0\sl-310\slmult0 \par\pard\ql\li1154\sb0\sl-310\slmult0 \par\pard\ql\li1154\
sb4\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1
.5.2 Reagents \par\pard\ql \li1860\sb130\sl-310\slmult0 \up0 \expndtw-4\charscal
ex100 \ul0\nosupersub\cf1\f2\fs27 (1) Trichloro acetic acid \par\pard\ql \li1845
\sb130\sl-310\slmult0 \up0 \expndtw-3\charscalex100 (2) diethyl ether \par\pard\
ql \li1845\sb170\sl-310\slmult0 \up0 \expndtw-7\charscalex100 ( 3 )Ethanol 9 5 %
(v /v ) \par\pard\ql \li1845\sb150\sl-310\slmult0 \up0 \expndtw-4\charscalex100
(4)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Cellulose powder
for TLC \par\pard\ql \li1845\sb170\sl-310\slmult0 \up0 \expndtw-5\charscalex100
(5)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Soluble starch \pa
r\pard\ql \li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb0\sl-310\slmult0 \par
\pard\ql\li1130\sb10\sl-310\slmult0 \up0 \expndtw-1\charscalex100 \ul0\nosupersu
b\cf14\f15\fs27 1.5.3\ul0\nosupersub\cf22\f23\fs27 \ul0\nosupersub\cf14\f15\fs2
7 Reference mixture\ul0\nosupersub\cf1\f2\fs27 -\par\pard\qj \li1761\sb0\sl-466
\slmult0 \par\pard\qj\li1761\ri727\sb36\sl-466\slmult0\fi69 \up0 \expndtw-4\char
scalex100 Dissolve in 100 ml water 200 mg of Sodium dihydrogen phosphate monohyd
rate \up0 \expndtw-5\charscalex100 ( Na H\ul0\sub\cf17\f18\fs26 2\ul0\nosupersub
\cf1\f2\fs27 PO\ul0\sub\cf17\f18\fs26 4\ul0\nosupersub\cf1\f2\fs27 , H\ul0\sub\
cf17\f18\fs26 2\ul0\nosupersub\cf1\f2\fs27 O), 300 mg of tetra sodium diphosphat
e decahydrate ( Na \ul0\sub\cf17\f18\fs26 4\ul0\nosupersub\cf1\f2\fs27 P\ul0\su
b\cf17\f18\fs26 2\ul0\nosupersub\cf1\f2\fs27 O\ul0\sub\cf17\f18\fs26 7\ul0\nosup
ersub\cf1\f2\fs27 , 10 \up0 \expndtw-4\charscalex100 H\ul0\sub\cf17\f18\fs26 2\u
l0\nosupersub\cf1\f2\fs27 O), 200 mg of penta sodium triphosphate ( Na \ul0\sub\
cf17\f18\fs26 5\ul0\nosupersub\cf1\f2\fs27 P\ul0\sub\cf17\f18\fs26 3\ul0\nosupe
rsub\cf1\f2\fs27 O \ul0\sub\cf17\f18\fs26 10\ul0\nosupersub\cf1\f2\fs27 ) and
200 mg of sodium \up0 \expndtw-5\charscalex100 hexametaphosphate ( NaPO\ul0\sub\
cf17\f18\fs26 3\ul0\nosupersub\cf1\f2\fs27 )\ul0\sub\cf17\f18\fs26 2 \ul0\nosup
ersub\cf1\f2\fs27 [lx >10] \par\pard\ql \li1845\sb143\sl-310\slmult0 \up0 \expn
dtw-4\charscalex100 The reference mixture is stable at 4 C for at least 4 weeks.
\par\pard\ql \li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb0\sl-310\slmult0
\par\pard\ql\li1130\sb10\sl-310\slmult0 \up0 \expndtw0\charscalex101 \ul0\nosupe
rsub\cf14\f15\fs27 1.5.4 Developing Solvent \par\pard\qj \li1831\ri884\sb18\sl-4
70\slmult0 \up0 \expndtw0\charscalex106 \ul0\nosupersub\cf1\f2\fs27 Mix 140 ml i

sopropyl alcohol, 40 ml of a 135 gm / litre solution of trichlor \up0 \expndtw0\

charscalex100 acetic acid and 0.6 ml of ammonium hydroxide 0.9 gm /ml, about 25
% (m/m) \up0 \expndtw0\charscalex100 solution. \par\pard\ql \li1130\sb0\sl-310\s
lmult0 \par\pard\ql\li1130\sb173\sl-310\slmult0 \up0 \expndtw-7\charscalex100 \u
l0\nosupersub\cf14\f15\fs27 1.5.5 Spray Reagent I \par\pard\ql \li1761\sb0\sl-31
0\slmult0 \par\pard\ql\li1761\sb180\sl-310\slmult0 \up0 \expndtw-6\charscalex100
\ul0\nosupersub\cf1\f2\fs27 Mix equal volume of a 75 gm / litre solution of amm
onium molybdate tetrahydrate \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\q
l\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\
li5867\sb92\sl-218\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf10\f1
1\fs19 15
{\shp {\*\shpinst\shpleft1106\shptop2128\shpright10828\shpbottom2596\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9722}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9722,468);(9722,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1106\shptop2596\shpright10828\shpbottom3061\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz53\shplid1
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9722}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9722,465);(9722,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1106\shptop3061\shpright10828\shpbottom3529\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz63\shplid2
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9722}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9722,468);(9722,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1125\shptop4132\shpright10828\shpbottom4446\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz78\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9703}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9703,314);(9703,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1831\shptop4590\shpright10828\shpbottom4902\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz105\shplid4
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8997}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(8997,312);(8997,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1816\shptop4902\shpright5784\shpbottom5370\shpfhdr0\shp
bxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz128\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 3968}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(3968,468);(3968,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1816\shptop5370\shpright5784\shpbottom5838\shpfhdr0\shp
bxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz142\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 3968}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(3968,468);(3968,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1816\shptop5843\shpright10828\shpbottom6308\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz161\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9012}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9012,465);(9012,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1816\shptop6308\shpright10828\shpbottom6776\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz192\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9012}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9012,468);(9012,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1104\shptop7252\shpright10828\shpbottom7722\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz222\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 470}}
{\sp{\sn pVerticies}{\sv 8;4;(0,470);(9724,470);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1732\shptop8200\shpright10828\shpbottom8665\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz274\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9096}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9096,465);(9096,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1732\shptop8665\shpright10828\shpbottom9133\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz294\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9096}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9096,468);(9096,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1732\shptop9133\shpright10828\shpbottom9601\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz366\shplid12
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9096}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9096,468);(9096,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1732\shptop9601\shpright10828\shpbottom10067\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz427\shplid13
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9096}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9096,466);(9096,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1816\shptop10072\shpright10828\shpbottom10540\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz481\shplid14
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9012}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9012,468);(9012,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop11017\shpright10828\shpbottom11483\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz505\shplid15
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9724,466);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop11483\shpright10828\shpbottom11951\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz532\shplid16
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9024,468);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop11951\shpright10828\shpbottom12419\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz558\shplid17
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9024,468);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop12419\shpright10828\shpbottom12884\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz582\shplid18
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9024,465);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1104\shptop13367\shpright10828\shpbottom13679\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz597\shplid19
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9724,312);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop13679\shpright10828\shpbottom14629\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz620\shplid20
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 950}}
{\sp{\sn pVerticies}{\sv 8;4;(0,950);(9724,950);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g18}{\bkmkend Pg18}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\s
b0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\qj \li1761\ri1004\sb372\sl-480\slmult0\fi69 \up0 \expndtw-7\charsca
lex100 \ul0\nosupersub\cf1\f2\fs27 [(NH\ul0\sub\cf17\f18\fs26 4\ul0\nosupersub\c
f1\f2\fs27 )\ul0\sub\cf17\f18\fs26 6\ul0\nosupersub\cf1\f2\fs27 Mo\ul0\sub\cf17\
f18\fs26 2\ul0\nosupersub\cf1\f2\fs27 )\ul0\sub\cf17\f18\fs26 24\ul0\nosupersub\
cf1\f2\fs27 -4H\ul0\sub\cf17\f18\fs26 2\ul0\nosupersub\cf1\f2\fs27 O] and conc n
itric acid ( 1.4gm / ml) and dissolve 10 gm tartaric \up0 \expndtw-8\charscalex1
00 acid in 100 ml of this mixture \par\pard\ql \li1130\sb0\sl-310\slmult0 \par\p
ard\ql\li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb121\sl-310\slmult0 \up0 \
expndtw0\charscalex102 \ul0\nosupersub\cf14\f15\fs27 1.5.6 \ul0\nosupersub\cf1\f
2\fs27 Spray reagent II \par\pard\ql \li1831\ri908\sb22\sl-465\slmult0 \up0 \exp
ndtw-4\charscalex100 Dissolve 0.5 gm of 1 amino 2 napthol- 4 sulphonic acid in a
mixture of 195 ml of \up0 \expndtw0\charscalex102 a l50 gm /l solution of sodiu
m disulphite ( Sod metabisulphite ) and 5 ml of a \up0 \expndtw-4\charscalex100
200 gm /1 solution of sodium sulphite (Na\ul0\sub\cf17\f18\fs26 2\ul0\nosupersub
\cf1\f2\fs27 S\ul0\sub\cf17\f18\fs26 2\ul0\nosupersub\cf1\f2\fs27 O\ul0\sub\cf17
\f18\fs26 3\ul0\nosupersub\cf1\f2\fs27 ). Dissolve 40 gm of sod acetate \up0 \ex
pndtw-4\charscalex100 trihydrate in the mixture. Store the reagent in a tightly
closed brown bottle in \up0 \expndtw-5\charscalex100 refrigerator, Discard the s
olution after 1 week. \par\pard\ql \li1130\sb0\sl-310\slmult0 \par\pard\ql\li113
0\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb4\sl-310\slmult0 \up0 \expndtw0\chars
calex102 \ul0\nosupersub\cf14\f15\fs27 1.5.6 \ul0\nosupersub\cf1\f2\fs27 Apparat
us \par\pard\ql \li1850\sb150\sl-310\slmult0 \up0 \expndtw-3\charscalex100 (1)\u
l0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Glass plates - 10 cm x
20 cm \par\pard\ql \li1850\sb150\sl-310\slmult0 \up0 \expndtw-4\charscalex100 (2
)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Spreading device for
preparing layers of 0.25 mm thickness \par\pard\ql \li1855\sb30\sl-310\slmult0
\up0 \expndtw-4\charscalex100 (3)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\
cf1\f2\fs27 Laboratory mixer \par\pard\ql \li1855\sb150\sl-310\slmult0 \up0 \exp
ndtw-4\charscalex100 (4)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs
27 Dessicator \par\pard\qj \li1855\ri1792\sb30\sl-480\slmult0 \up0 \expndtw-1\ch
arscalex100 (5)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Mechan
ical meat mincer fitted with a plate with holes
of diameter \up0 \expndtw-4\c

harscalex100 not more than 4 mm. \par\pard\ql \li1855\sb121\sl-310\slmult0 \up0

\expndtw-6\charscalex100 (6)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f
2\fs27 Fluted filter paper, 15 cm dia \par\pard\ql \li1855\sb170\sl-310\slmult0
\up0 \expndtw-4\charscalex100 (7)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\
cf1\f2\fs27 Micropipette - 1 l or micrometer syringe \par\pard\ql \li1855\sb150\s
l-310\slmult0 \up0 \expndtw-4\charscalex100 (8)\ul0\nosupersub\cf15\f16\fs27 \u
l0\nosupersub\cf1\f2\fs27 Paper lined glass tank. \par\pard\ql \li1855\sb170\sl310\slmult0 \up0 \expndtw-4\charscalex100 (9)\ul0\nosupersub\cf15\f16\fs27 \ul0
\nosupersub\cf1\f2\fs27 Hair dryer \par\pard\ql \li1855\sb170\sl-310\slmult0 \up
0 \expndtw-4\charscalex100 (10)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf
1\f2\fs27 Sprayer \par\pard\ql \li1855\sb170\sl-310\slmult0 \up0 \expndtw-4\char
scalex100 (11)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Oven ca
pable of being maintained at 60 C \par\pard\ql \li1149\sb0\sl-310\slmult0 \par\p
ard\ql\li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb10\sl-310\slmult0 \up0 \e
xpndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.5.7 Preparation of TLC pl
ates \par\pard\qj \li1831\ri1287\sb47\sl-460\slmult0 \up0 \expndtw-4\charscalex1
00 \ul0\nosupersub\cf1\f2\fs27 Dissolve 0.3 gm starch in 90 ml boiling water, ad
d 15 gm of cellulose powder \line \up0 \expndtw-4\charscalex100 and homogenize i
n the laboratory mixer for 1 minute. Apply the slurry onto \par\pard\ql \li5867\
sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb
84\sl-218\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf10\f11\fs19 16
{\shp {\*\shpinst\shpleft1732\shptop2128\shpright10828\shpbottom2598\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9096}}{\sp{\sn geoBottom}{\sv 470}}
{\sp{\sn pVerticies}{\sv 8;4;(0,470);(9096,470);(9096,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1732\shptop2598\shpright10828\shpbottom3066\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz123\shplid1
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9096}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9096,468);(9096,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop3548\shpright10828\shpbottom4014\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz137\shplid2
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9724,466);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop4014\shpright10828\shpbottom4482\shpfhdr0\sh

pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz168\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9024,468);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop4482\shpright10828\shpbottom4950\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz203\shplid4
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9024,468);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop4950\shpright10828\shpbottom5416\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz241\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9024,466);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop5416\shpright10828\shpbottom5884\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz291\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9024,468);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop5884\shpright10828\shpbottom6352\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz305\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9024,468);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1104\shptop6832\shpright10828\shpbottom7295\shpfhdr0\sh

pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz320\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9724}}{\sp{\sn geoBottom}{\sv 463}}
{\sp{\sn pVerticies}{\sv 8;4;(0,463);(9724,463);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1821\shptop7295\shpright10828\shpbottom7758\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz348\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9007}}{\sp{\sn geoBottom}{\sv 463}}
{\sp{\sn pVerticies}{\sv 8;4;(0,463);(9007,463);(9007,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1821\shptop7758\shpright10828\shpbottom8219\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz387\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9007}}{\sp{\sn geoBottom}{\sv 461}}
{\sp{\sn pVerticies}{\sv 8;4;(0,461);(9007,461);(9007,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1826\shptop8219\shpright10828\shpbottom8533\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz417\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9002}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9002,314);(9002,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1826\shptop8533\shpright10828\shpbottom8996\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz447\shplid12
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9002}}{\sp{\sn geoBottom}{\sv 463}}
{\sp{\sn pVerticies}{\sv 8;4;(0,463);(9002,463);(9002,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1826\shptop8996\shpright10828\shpbottom9505\shpfhdr0\sh

pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz480\shplid13
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9002}}{\sp{\sn geoBottom}{\sv 509}}
{\sp{\sn pVerticies}{\sv 8;4;(0,509);(9002,509);(9002,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1826\shptop9505\shpright10828\shpbottom9971\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz510\shplid14
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9002}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9002,466);(9002,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1826\shptop9971\shpright10828\shpbottom10444\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz524\shplid15
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9002}}{\sp{\sn geoBottom}{\sv 473}}
{\sp{\sn pVerticies}{\sv 8;4;(0,473);(9002,473);(9002,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1826\shptop10444\shpright10828\shpbottom10916\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz558\shplid16
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9002}}{\sp{\sn geoBottom}{\sv 472}}
{\sp{\sn pVerticies}{\sv 8;4;(0,472);(9002,472);(9002,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1826\shptop10916\shpright10828\shpbottom11389\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz604\shplid17
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9002}}{\sp{\sn geoBottom}{\sv 473}}
{\sp{\sn pVerticies}{\sv 8;4;(0,473);(9002,473);(9002,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1826\shptop11389\shpright10828\shpbottom11857\shpfhdr0\

shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz634\shplid18
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9002}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9002,468);(9002,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1826\shptop11884\shpright10828\shpbottom12349\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz668\shplid19
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9002}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9002,465);(9002,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1826\shptop12349\shpright10828\shpbottom12827\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz699\shplid20
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9002}}{\sp{\sn geoBottom}{\sv 478}}
{\sp{\sn pVerticies}{\sv 8;4;(0,478);(9002,478);(9002,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1120\shptop13297\shpright10828\shpbottom13765\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz729\shplid21
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9708}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9708,468);(9708,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop13770\shpright10816\shpbottom14240\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz759\shplid22
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9012}}{\sp{\sn geoBottom}{\sv 470}}
{\sp{\sn pVerticies}{\sv 8;4;(0,470);(9012,470);(9012,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop14240\shpright10816\shpbottom14706\shpfhdr0\

shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz770\shplid23
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9012}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9012,466);(9012,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g19}{\bkmkend Pg19}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\s
b0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\qj \li1831\sb0\sl-466\slmult0 \par\pard\qj\li1831\ri1091\sb58\sl-46
6\slmult0 \up0 \expndtw0\charscalex100 \ul0\nosupersub\cf1\f2\fs27 glass plates
with the spreading device adjusted to obtain a layer of 0.25 mm. \line \up0 \exp
ndtw0\charscalex104 Air dry the plates at room temperature and heat them finally
for 10 minutes \line \up0 \expndtw-3\charscalex100 at 100 \ul0\super\cf17\f18\f
s26 0\ul0\nosupersub\cf1\f2\fs27 C. Store the plates in a dessicator. Alternati
vely ready to use plates may \line \up0 \expndtw-4\charscalex100 be used. \par\p
ard\ql \li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb273\sl-310\slmult0 \up0
\expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.5.8 Preparation of seru
m \par\pard\ql \li1831\sb0\sl-466\slmult0 \par\pard\ql\li1831\ri860\sb36\sl-466\
slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Macerate 50 gm
of test sample with 15 ml water at 40 - 60 \ul0\super\cf17\f18\fs26 0\ul0\nosup
ersub\cf1\f2\fs27 C in a beaker with a \up0 \expndtw-4\charscalex100 spatula or
flattened stirring rod until a homogeneous mass is obtained taking no \up0 \exp
ndtw-5\charscalex100 more than 5 minutes. Add 10 gm of trichloracetic acid and m
ix again. \line \up0 \expndtw-4\charscalex100 Immediately place in a refrigerato
r for 1 hr and then collect the separated serum \up0 \expndtw-3\charscalex100 by
decanting through the fluted filter paper. If the filterate is turbid shake onc
e \up0 \expndtw-3\charscalex100 with an equal volume of diethyl ether. Remove th
e ether layer with a small pipette \up0 \expndtw-4\charscalex100 and add an equa
l volume of ethanol to the aqueous phase. \par\pard\qj \li1831\ri1838\sb19\sl-46
0\slmult0 \up0 \expndtw-3\charscalex100 Shake for 1 minute. Allow the mixture to
stand for few minutes and filter \up0 \expndtw-4\charscalex100 through a fluted
filter paper. \par\pard\ql \li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb0\s
l-310\slmult0 \par\pard\ql\li1149\sb4\sl-310\slmult0 \up0 \expndtw-4\charscalex1
00 \ul0\nosupersub\cf14\f15\fs27 1.5.9 Chromatographic separation \par\pard\ql \
li1831\sb0\sl-466\slmult0 \par\pard\ql\li1831\ri815\sb16\sl-466\slmult0 \up0 \ex
pndtw-4\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Pour developing solvent in the
developing tank to a depth of 5 - 10 mm and close \line \up0 \expndtw-3\charsca
lex100 the tank with its lid. Allow to stand for at least 30 minutes at ambient
\line \up0 \expndtw-4\charscalex100 temperature. Apply 3 l of serum or 6 l if the
clearing procedure was carried \line \up0 \expndtw-4\charscalex100 out to the ce
llulose layer on a pencil line drawn at about 2 cm from the bottom. \line \up0 \
expndtw-4\charscalex100 Keep the spots small by applying 1 l at a time. Use a war
m air stream from hair \line \up0 \expndtw-3\charscalex100 dryer for drying. In
the same way apply 3 l of reference
mixture to the plate at \line \up0 \expndt
w-3\charscalex100 a distance of 1 - 1.5 cm from the sample spot. Remove the lid
from the tank and \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb
0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\
sl-218\slmult0 \par\pard\ql\li5867\sb67\sl-218\slmult0 \up0 \expndtw-3\charscale
x100 \ul0\nosupersub\cf10\f11\fs19 17
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}}
{\shp {\*\shpinst\shpleft1804\shptop13823\shpright10828\shpbottom14288\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz421\shplid21
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9024,465);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g20}{\bkmkend Pg20}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\s
b0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li1831\sb0\sl-466\slmult0 \par\pard\ql\li1831\ri908\sb58\sl-466
\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf1\f2\fs27 quickly place
the cellulose plate in the tank. Develop the plate until the solvent \line \up0
\expndtw-5\charscalex100 front has ascended to approx 10 cm from the pencil lin
e. Remove the plate, dry \line \up0 \expndtw-4\charscalex100 for 10 minutes in o
ven at 60 \ul0\super\cf17\f18\fs26 0\ul0\nosupersub\cf1\f2\fs27 C or for 30 min
utes at room temperature. Spray the \line \up0 \expndtw-4\charscalex100 plates l
ightly but uniformly with spray reagent No 1. Yellow spots appear \line \up0 \ex
pndtw-4\charscalex100 immediately. Dry the plate in a stream of warm air from a
hair dryer and heat in \line \up0 \expndtw-4\charscalex100 oven for 1 hr at 100
\ul0\super\cf17\f18\fs26 0\ul0\nosupersub\cf1\f2\fs27 C to remove last traces o
f nitric acid. Check that the plate \line \up0 \expndtw-4\charscalex100 is free
from pungent smell of nitric acid. Allow the plate to cool and spray lightly \li
ne \up0 \expndtw-2\charscalex100 with spray reagent no 2. Blue spots appear imme
diately. Spraying with reagent 2 \line \up0 \expndtw-3\charscalex100 is not an a
bsolute necessity but the intense blue spots produced improve the \line \up0 \ex
pndtw-4\charscalex100 detection considerably. \par\pard\ql \li1149\sb0\sl-310\sl
mult0 \par\pard\ql\li1149\sb293\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul
0\nosupersub\cf14\f15\fs27 1.5.10 Interpretation \par\pard\ql \li1831\sb0\sl-473
\slmult0 \par\pard\ql\li1831\ri1099\sb3\sl-473\slmult0 \up0 \expndtw-3\charscale
x100 \ul0\nosupersub\cf1\f2\fs27 Compare the migration distance of the phosphate
spots from the sample and the \up0 \expndtw-4\charscalex100 reference mixture.
An orthophosphate spot is always present. If the sample \up0 \expndtw-4\charscal
ex100 contains condensed phosphates, a diphosphate spot and / or spots of more \
line \up0 \expndtw-5\charscalex100 highly polymerized phosphates are visible. \p
ar\pard\qj \li1831\sb0\sl-460\slmult0 \par\pard\qj\li1831\sb0\sl-460\slmult0 \pa
r\pard\qj\li1831\ri1156\sb38\sl-460\slmult0 \up0 \expndtw-4\charscalex100 ( Ref:
- I.S 5960 ( Part 13) : 1988 / I.S.O 5553 : 1980 Meat and Meat Products -\line \
up0 \expndtw-5\charscalex100 Methods of Test - Detection of Polyphosphates ) \pa
r\pard\ql \li1144\sb0\sl-310\slmult0 \par\pard\ql\li1144\sb0\sl-310\slmult0 \par
\pard\ql\li1144\sb0\sl-310\slmult0 \par\pard\ql\li1144\sb154\sl-310\slmult0 \up0
\expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.6 Determination of Glu
cono- delta- lactone (rule 72) \ul0\nosupersub\cf1\f2\fs27 :-\par\pard\ql \li183
1\sb0\sl-310\slmult0 \par\pard\ql\li1831\sb0\sl-310\slmult0 \par\pard\ql\li1831\
sb10\sl-310\slmult0 \up0 \expndtw-3\charscalex100 This is an enzyme ultraviolet

procedure recommended by I.S.O and B.S.I (I. \par\pard\ql \li5867\sb0\sl-218\slm

ult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmul
t0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb54\sl-218\slmult
0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf10\f11\fs19 18
{\shp {\*\shpinst\shpleft1804\shptop2128\shpright10828\shpbottom2596\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1804\shptop2596\shpright10828\shpbottom3061\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9024,465);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1804\shptop3061\shpright10828\shpbottom3529\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9024,468);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop3529\shpright10828\shpbottom3997\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz95\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9024,468);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop3997\shpright10828\shpbottom4463\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz109\shplid4
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,466);(9024,466);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}

{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772

15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop4463\shpright10828\shpbottom4931\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz123\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9024,468);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop4931\shpright10828\shpbottom5399\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz143\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9024,468);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop5399\shpright10828\shpbottom5864\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz157\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9024,465);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft1804\shptop5864\shpright10828\shpbottom6332\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz171\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9024}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9024,468);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1804\shptop6332\shpright10828\shpbottom6800\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz181\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9024,468);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772

15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft1120\shptop7393\shpright10828\shpbottom7705\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz196\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9708,312);(9708,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1804\shptop8202\shpright10828\shpbottom8672\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz223\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,470);(9024,470);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1804\shptop8672\shpright10828\shpbottom9148\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,476);(9024,476);(9024,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1804\shptop9148\shpright10324\shpbottom9618\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,470);(8520,470);(8520,0);(0,0)}}
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}}
{\shp {\*\shpinst\shpleft1804\shptop9618\shpright10324\shpbottom10091\shpfhdr0\s
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772

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}}
{\shp {\*\shpinst\shpleft1804\shptop11034\shpright10828\shpbottom11502\shpfhdr0\
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{\shp {\*\shpinst\shpleft1804\shptop11502\shpright10828\shpbottom11970\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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}}
{\shp {\*\shpinst\shpleft1116\shptop13043\shpright10828\shpbottom13357\shpfhdr0\
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{\shp {\*\shpinst\shpleft1804\shptop13847\shpright10324\shpbottom14320\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,473);(8520,473);(8520,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g21}{\bkmkend Pg21}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\s
b0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li1831\sb0\sl-310\slmult0 \par\pard\ql\li1831\sb0\sl-310\slmult
0 \par\pard\ql\li1831\sb32\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nos
upersub\cf1\f2\fs27 S.O 4133 and B.S 4401, part 13). A test combination kit is a

vailable. \par\pard\ql \li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb0\sl-310

\slmult0 \par\pard\ql\li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb0\sl-310\s
lmult0 \par\pard\ql\li1149\sb290\sl-310\slmult0 \up0 \expndtw-5\charscalex100 \u
l0\nosupersub\cf14\f15\fs27 1.6.1 Reagents \par\pard\qj \li1490\ri2005\sb470\sl480\slmult0\tx1874 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 (1)
\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Perchloric acid - 0.4
M - Dilute 17.3 ml Perchloric acid (70 % m /m) to \line\tab \up0 \expndtw-4\cha
rscalex100 500 ml with water \par\pard\qj \li1490\ri1943\sb0\sl-480\slmult0\tx18
74 \up0 \expndtw-1\charscalex100 (2)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupers
ub\cf1\f2\fs27 Potassium hydroxide - 2 M - Dissolve 56.l gm Pot. Hydroxide in wa
ter \line\tab \up0 \expndtw-2\charscalex100 - Dilute to 500 ml \par\pard\ql \li1
490\ri1818\sb0\sl-470\slmult0\tx1874\tx1874 \up0 \expndtw-2\charscalex100 (3)\ul
0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Buffer Solution - pH 8.0
- Dissolve 2.64 gm glycylglycine and 0.284 gm \line\tab \up0 \expndtw-3\charsca
lex100 magnesium chloride hexahydrate in 150 ml water. Adjust to pH 8 with \line
\tab \up0 \expndtw-3\charscalex100 potassium hydroxide. Dilute to 200 ml with w
ater \par\pard\qj \li1490\ri2210\sb0\sl-460\slmult0\tx1874 \up0 \expndtw-4\chars
calex100 (4)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Nicotinam
ide adenine dinucleotide phosphate ( NADP) - Dissolve 50 \line\tab \up0 \expndtw
-4\charscalex100 mg of NADP disodium salt in 5 ml water. \par\pard\qj \li1490\ri
1996\sb8\sl-460\slmult0\tx1874 \up0 \expndtw-4\charscalex100 (5)\ul0\nosupersub\
cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Adenosine -5- triphosphate(ATP) - Dis
solve250 mg ATP disodium salt \line\tab \up0 \expndtw-4\charscalex100 and 250 mg
sodium hydrogen carbonate in 5 ml water. \par\pard\qj \li1495\ri1741\sb0\sl-480
\slmult0\tx1883 \up0 \expndtw-4\charscalex100 (6)\ul0\nosupersub\cf15\f16\fs27
\ul0\nosupersub\cf1\f2\fs27 6 - Phosphogluconate dehydrogenase (6 PGDH)- Commerc
ial suspension \line\tab \up0 \expndtw-4\charscalex100 containing 2 mg 6- PGDH /
ml from yeast \par\pard\ql \li1495\sb105\sl-310\slmult0 \up0 \expndtw-2\charsca
lex100 (7)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Gluconate k
inase ( GK)- Suspension containingl mg / ml from E.Coli. \par\pard\ql \li1149\sb
0\sl-310\slmult0 \par\pard\ql\li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb30
\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.6.
2 Procedure \par\pard\ql \li1130\ri915\sb422\sl-466\slmult0 \up0 \expndtw-2\char
scalex100 \ul0\nosupersub\cf1\f2\fs27 Weigh 50 gm of prepared sample into a homo
genizer. Add 100 ml of cold (0 C) 0.4 M \line \up0 \expndtw-3\charscalex100 perch
loric acid and homogenize. Transfer the slurry to a 100 ml centrifuge tube. \lin
e \up0 \expndtw-2\charscalex100 Centrifuge at 3000 rpm for 10 minutes. Move the
fat layer. Decant the supernatant \line \up0 \expndtw-2\charscalex100 through a
fluted filter paper into a 200 ml conical flask and discard the first 10 ml. \pa
r\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par
\pard\ql\li5867\sb103\sl-218\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupers
ub\cf10\f11\fs19 19
{\shp {\*\shpinst\shpleft1804\shptop2128\shpright10324\shpbottom2600\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1120\shptop3500\shpright10828\shpbottom4405\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1461\shptop4904\shpright10828\shpbottom5372\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1461\shptop5372\shpright10828\shpbottom5840\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1461\shptop5840\shpright10828\shpbottom6311\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1461\shptop6311\shpright10828\shpbottom6779\shpfhdr0\sh
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{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}

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{\shp {\*\shpinst\shpleft1461\shptop7252\shpright10828\shpbottom7717\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1461\shptop7717\shpright10828\shpbottom8185\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1461\shptop8185\shpright10828\shpbottom8656\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1461\shptop8656\shpright10828\shpbottom9124\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1461\shptop9124\shpright10828\shpbottom9596\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1461\shptop9596\shpright10828\shpbottom10062\shpfhdr0\s
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{\shp {\*\shpinst\shpleft1466\shptop10062\shpright10828\shpbottom10530\shpfhdr0\
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{\shp {\*\shpinst\shpleft1466\shptop11003\shpright10828\shpbottom11468\shpfhdr0\
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{\shp {\*\shpinst\shpleft1104\shptop14216\shpright10828\shpbottom14684\shpfhdr0\
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g22}{\bkmkend Pg22}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\s
b0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li1130\sb0\sl-464\slmult0 \par\pard\ql\li1130\ri1009\sb61\sl-46
4\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Transfer 50
ml of the filterate to a 100 ml beaker. Adjust to pH 10 with Pot. Hydroxide \lin
e \up0 \expndtw-2\charscalex100 and make upto 100 ml in a volumetric flask with
water. Cool in ice for 20 minutes. \line \up0 \expndtw-2\charscalex100 Filter th
rough a fluted filter paper. Discard the first 10 ml. Pipette 25 ml of the filtr
ate \line \up0 \expndtw-2\charscalex100 (V ml) into a 250 ml volumetric flask. D
ilute to mark with water ( maximum \line \up0 \expndtw-2\charscalex100 concentra
tion of D (+) gluconate is 400 mg /1). this is the prepared extract.\ul0\nosuper
sub\cf10\f11\fs19 \ul0\nosupersub\cf1\f2\fs27 Pipette into \line \up0 \expndtw2\charscalex100 each of 2 photometric cells - 2.5 ml of pH 8 buffer, 0.1 ml NADP
, 0.1 ml ATP. \par\pard\ql \li1130\ri959\sb435\sl-466\slmult0 \up0 \expndtw-2\ch
arscalex100 Into one of the cells pipette 0.2 ml extract, into the other 0.20 ml
water. Pipette 0.05 ml \up0 \expndtw-3\charscalex100 of 6 - PGDH suspension on
to a plastic spatula, mix with the contents of one of the \up0 \expndtw-2\charsc
alex100 cells. Repeat the operation with the second cell. Read the absorbance of
each cell \line \up0 \expndtw-3\charscalex100 against air at 365 nm after 5 min
utes. Retain the cells for reaction \par\pard\qj \li2551\ri4620\sb463\sl-480\slm
ult0 \up0 \expndtw-4\charscalex100 Al = absorbance of test solution A AIB = \up0
\expndtw-4\charscalex100 absorbance of blank \par\pard\ql \li1135\ri1055\sb0\sl
-475\slmult0 \up0 \expndtw-2\charscalex100 Pipette 0.01 ml of GK suspension on t
o the plastic spatula. Mix with the contents of \up0 \expndtw-3\charscalex100 on
e of the cells. Repeat the operation with the other cell. Read the absorbance of
each \up0 \expndtw-3\charscalex100 cell at 365 nm after 10 minutes and again af
ter 2 minutes until a constant rate of \line \up0 \expndtw-3\charscalex100 absor
bance is obtained. Plot the absorbance against time and extrapolate the linear p
art \up0 \expndtw-4\charscalex100 of the curve back to zero time. \par\pard\ql \
li2551\sb0\sl-310\slmult0 \par\pard\ql\li2551\sb257\sl-310\slmult0 \up0 \expndtw
-3\charscalex100 A2 = Absorbance (T = 0) of the test solution \par\pard\ql \li25
51\sb0\sl-310\slmult0 \par\pard\ql\li2551\sb0\sl-310\slmult0 \par\pard\ql\li2551
\sb10\sl-310\slmult0 \up0 \expndtw-5\charscalex100 A2 B = Absorbance (T = 0) of
the blank solution \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\s
b0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0
\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\s
l-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-21
8\slmult0 \par\pard\ql\li5867\sb206\sl-218\slmult0 \up0 \expndtw-2\charscalex100
\ul0\nosupersub\cf10\f11\fs19 20
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{\shp {\*\shpinst\shpleft1104\shptop3529\shpright10828\shpbottom3997\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1104\shptop4463\shpright10828\shpbottom4931\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1104\shptop6294\shpright10828\shpbottom6762\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft2522\shptop7691\shpright7298\shpbottom8164\shpfhdr0\shp
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}}
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g23}{\bkmkend Pg23}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\s
b0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb0\sl-310\slmult
0 \par\pard\ql\li1149\sb0\sl-310\slmult0 \par\pard\ql\li1149\sb82\sl-310\slmult0
\up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.6.3 Calculation \
par\pard\ql \li1850\sb0\sl-310\slmult0 \par\pard\ql\li1850\sb0\sl-310\slmult0 \p
ar\pard\ql\li1850\sb30\sl-310\slmult0 \up0 \expndtw0\charscalex101 \ul0\nosupers
ub\cf1\f2\fs27 ? A = (A2 - Al) - (A2B - A1B) \par\pard\ql \li1144\sb0\sl-310\slm

ult0 \par\pard\ql\li1144\sb300\sl-310\slmult0 \up0 \expndtw-4\charscalex100 Gluc

ona - delta lactone % by mass = 15058 x ? A (100 + M x m) \par\pard\li5640\sb1
71\sl-310\slmult0\fi0\tx7713 \up0 \expndtw0\charscalex102 V x m\tab \up0 \expndt
w0\charscalex102 (100 )\par\pard\ql \li1850\sb0\sl-310\slmult0 \par\pard\ql\li18
50\sb0\sl-310\slmult0 \par\pard\ql\li1850\sb9\sl-310\slmult0 \up0 \expndtw-5\cha
rscalex100 Where V = volume in ml of filtrate to make prepared extract M = \par\
pard\qj \li2616\ri2237\sb27\sl-460\slmult0 \up0 \expndtw-4\charscalex100 moistur
e content of prepared sample percent m/m m = mass in \up0 \expndtw-4\charscalex1
00 gm of test sample. \par\pard\ql \li1144\sb0\sl-310\slmult0 \par\pard\ql\li114
4\sb294\sl-310\slmult0 \up0 \expndtw-3\charscalex100 (Ref:- Pearsons Composition
and Analysis of Foods 9\ul0\super\cf17\f18\fs26 th\ul0\nosupersub\cf1\f2\fs27
edn1991 , page 502) \par\pard\ql \li1140\sb0\sl-310\slmult0 \par\pard\ql\li1140\
sb0\sl-310\slmult0 \par\pard\ql\li1140\sb0\sl-310\slmult0 \par\pard\ql\li1140\sb
0\sl-310\slmult0 \par\pard\ql\li1140\sb0\sl-310\slmult0 \par\pard\ql\li1140\sb0\
sl-310\slmult0 \par\pard\ql\li1140\sb30\sl-310\slmult0 \up0 \expndtw-5\charscale
x100 \ul0\nosupersub\cf14\f15\fs27 2.0 Additional tests as per label declaration
if necessary \par\pard\ql \li1140\sb0\sl-310\slmult0 \par\pard\ql\li1140\sb140\
sl-310\slmult0 \up0 \expndtw-4\charscalex100 2.1Total Fat \ul0\nosupersub\cf1\f2
\fs27 :-\par\pard\ql \li1130\ri893\sb420\sl-468\slmult0 \up0 \expndtw-4\charscal
ex100 Weigh accurately 3-4gms of well mixed sample in a 100ml beaker. Add a few
drops of \line \up0 \expndtw-4\charscalex100 NH\ul0\sub\cf17\f18\fs26 4\ul0\nosu
persub\cf1\f2\fs27 OH and warm on a steam bath. Add 10ml of cone HCl and boil f
or approx 30 \line \up0 \expndtw-2\charscalex100 minutes. Cool, filter through a
wetted filter paper. Wash filter paper with hot water. \line \up0 \expndtw-3\ch
arscalex100 Dry the filter paper containing the residue of the sample, roll and
insert in an \line \up0 \expndtw-2\charscalex100 extraction thimble and extract
fat in a soxhlet apparatus using ethyl ether or petroleum \line \up0 \expndtw-2\
charscalex100 ether, transfer to another flask. Remove solvent. Keep flask in an
air oven maintained \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867
\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\s
b0\sl-218\slmult0 \par\pard\ql\li5867\sb67\sl-218\slmult0 \up0 \expndtw-3\charsc
alex100 \ul0\nosupersub\cf10\f11\fs19 21
{\shp {\*\shpinst\shpleft1120\shptop2128\shpright10828\shpbottom3066\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,938);(9708,938);(9708,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1821\shptop3066\shpright10828\shpbottom4007\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz57\shplid1
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9007}}{\sp{\sn geoBottom}{\sv 941}}
{\sp{\sn pVerticies}{\sv 8;4;(0,941);(9007,941);(9007,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1116\shptop4007\shpright10828\shpbottom4945\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz101\shplid2
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

\sn geoRight}{\sv 9712}}{\sp{\sn geoBottom}{\sv 938}}

{\sp{\sn pVerticies}{\sv 8;4;(0,938);(9712,938);(9712,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft5359\shptop4789\shpright6715\shpbottom4809\shpfhdr0\shp
bxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz130\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 1356}}{\sp{\sn geoBottom}{\sv 20}}
{\sp{\sn pVerticies}{\sv 8;4;(0,20);(1356,20);(1356,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 0}}{\s
p{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv 1}}{\s
p{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft7675\shptop4789\shpright8515\shpbottom4809\shpfhdr0\shp
bxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz158\shplid4
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 840}}{\sp{\sn geoBottom}{\sv 20}}
{\sp{\sn pVerticies}{\sv 8;4;(0,20);(840,20);(840,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 0}}{\s
p{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv 1}}{\s
p{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft5611\shptop4974\shpright10828\shpbottom5286\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz168\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 5217}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(5217,312);(5217,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1821\shptop5785\shpright9597\shpbottom6256\shpfhdr0\shp
bxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz219\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 7776}}{\sp{\sn geoBottom}{\sv 471}}
{\sp{\sn pVerticies}{\sv 8;4;(0,471);(7776,471);(7776,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1821\shptop6256\shpright9597\shpbottom6724\shpfhdr0\shp
bxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz234\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

\sn geoRight}{\sv 7776}}{\sp{\sn geoBottom}{\sv 468}}

{\sp{\sn pVerticies}{\sv 8;4;(0,468);(7776,468);(7776,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1821\shptop6724\shpright9597\shpbottom7189\shpfhdr0\shp
bxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz248\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 7776}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(7776,465);(7776,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1116\shptop7787\shpright10828\shpbottom8101\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz263\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9712}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9712,314);(9712,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop8101\shpright10828\shpbottom8413\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz303\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9717}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9717,312);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop8413\shpright10828\shpbottom8728\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz312\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9717}}{\sp{\sn geoBottom}{\sv 315}}
{\sp{\sn pVerticies}{\sv 8;4;(0,315);(9717,315);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop8728\shpright10828\shpbottom9040\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz320\shplid12
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

\sn geoRight}{\sv 9717}}{\sp{\sn geoBottom}{\sv 312}}

{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9717,312);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop9040\shpright10828\shpbottom9354\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz328\shplid13
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9717}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9717,314);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop9354\shpright10828\shpbottom9666\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz336\shplid14
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9717}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9717,312);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop9666\shpright10828\shpbottom9980\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz344\shplid15
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9717}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9717,314);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop9980\shpright10828\shpbottom10295\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz352\shplid16
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,315);(9717,315);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1111\shptop10295\shpright10828\shpbottom10518\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz367\shplid17
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

\sn geoRight}{\sv 9717}}{\sp{\sn geoBottom}{\sv 223}}

{\sp{\sn pVerticies}{\sv 8;4;(0,223);(9717,223);(9717,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1111\shptop10518\shpright10828\shpbottom10741\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz375\shplid18
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,223);(9717,223);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1111\shptop10741\shpright10828\shpbottom11056\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz383\shplid19
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,315);(9717,315);(9717,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1104\shptop11480\shpright10828\shpbottom11948\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz407\shplid20
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9724,468);(9724,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1104\shptop11948\shpright10828\shpbottom12416\shpfhdr0\
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{\shp {\*\shpinst\shpleft1104\shptop12416\shpright10828\shpbottom12882\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

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{\shp {\*\shpinst\shpleft1104\shptop12882\shpright10828\shpbottom13350\shpfhdr0\
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{\shp {\*\shpinst\shpleft1104\shptop13350\shpright10828\shpbottom13818\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1104\shptop13818\shpright10828\shpbottom14284\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g24}{\bkmkend Pg24}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\s
b0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\qj \li1130\sb0\sl-460\slmult0 \par\pard\qj\li1130\ri1847\sb68\sl-46
0\slmult0 \up0 \expndtw1\charscalex100 \ul0\nosupersub\cf1\f2\fs27 at 100C for 30
minutes to remove residual solvent if any. Transfer flask to a \up0 \expndtw-2\
charscalex100 desiccator to allow it to cool. Weigh the residue and calculate to
tal fat. \par\pard\qj \li1130\sb0\sl-480\slmult0 \par\pard\qj\li1130\sb0\sl-480\
slmult0 \par\pard\qj\li1130\ri1705\sb444\sl-480\slmult0 \up0 \expndtw-2\charscal
ex100 (Ref:- ISO 1443- 1973 Codex approved method - Extraction / gravimetry Type
I \up0 \expndtw-3\charscalex100 method) \par\pard\qj \li1130\ri1346\sb17\sl-460
\slmult0 \up0 \expndtw0\charscalex103 PI also see I.S: 5960 (Part 3) 1970 Method
s of test for meat and meat products -\line \up0 \expndtw-2\charscalex100 Determ
ination of total fat content \par\pard\ql \li1135\sb0\sl-310\slmult0 \par\pard\q

l\li1135\sb294\sl-310\slmult0 \up0 \expndtw-5\charscalex100 \ul0\nosupersub\cf14

\f15\fs27 2.2Total Protein (Kjeldahl method) :-2.2.1 \par\pard\ql \li1135\sb0\sl
-310\slmult0 \par\pard\ql\li1135\sb0\sl-310\slmult0 \par\pard\ql\li1135\sb10\sl310\slmult0 \up0 \expndtw-6\charscalex100 Reagents \ul0\nosupersub\cf1\f2\fs27 :
-\par\pard\qj \li1845\sb0\sl-460\slmult0 \par\pard\qj\li1845\ri2618\sb47\sl-460\
slmult0 \up0 \expndtw-3\charscalex100 Kjeldahl catalyst:- 15gm Pot. Sulphate + 0
.5gm Copper sulphate \up0 \expndtw-3\charscalex100 Sulphuric Acid - Concentrated
\par\pard\ql \li1845\sb124\sl-310\slmult0 \up0 \expndtw-5\charscalex100 NaOH so
lution- 50% (1+1). Let stand until clear \par\pard\ql \li2966\sb130\sl-310\slmul
t0 \up0 \expndtw-4\charscalex100 Standard NaOHsolution-0.1 N=0.1 M (4.00gm/litre
) \par\pard\qj \li1845\ri1886\sb47\sl-460\slmult0 \up0 \expndtw-4\charscalex100
Standard acid solution- Prepare either HCl or H\ul0\sub\cf17\f18\fs26 2\ul0\nosu
persub\cf1\f2\fs27 SO\ul0\sub\cf17\f18\fs26 4\ul0\nosupersub\cf1\f2\fs27 soluti
on HCl sol-0.1 \up0 \expndtw-5\charscalex100 N= 0.1 M (3.646gm/litre) \par\pard\
ql \li1845\sb124\sl-310\slmult0 \up0 \expndtw-4\charscalex100 H\ul0\sub\cf17\f18
\fs26 2\ul0\nosupersub\cf1\f2\fs27 SO\ul0\sub\cf17\f18\fs26 4\ul0\nosupersub\cf1
\f2\fs27 sol - 0.1N=0.05 M (4.9gm/litre) \par\pard\ql \li1845\sb170\sl-310\slmu
lt0 \up0 \expndtw-5\charscalex100 Methyl Red Indicator - 0.5gm in 100ml ethanol
\par\pard\ql \li1140\sb0\sl-310\slmult0 \par\pard\ql\li1140\sb300\sl-310\slmult0
\up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 2.2.2 Procedure \ul
0\nosupersub\cf1\f2\fs27 :-\par\pard\ql \li1130\sb0\sl-310\slmult0 \par\pard\ql\
li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb10\sl-310\slmult0 \up0 \expndtw3\charscalex100 Weigh 1-1.5 gm of prepared sample and transfer to a kjeldahl dig
estion flask. Add \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb
0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\
sl-218\slmult0 \par\pard\ql\li5867\sb94\sl-218\slmult0 \up0 \expndtw-3\charscale
x100 \ul0\nosupersub\cf10\f11\fs19 22
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15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g25}{\bkmkend Pg25}\par\pard\ql \li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\s
b0\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0
\sl-184\slmult0 \par\pard\ql\li6230\sb0\sl-184\slmult0 \par\pard\ql\li6230\sb0\s
l-184\slmult0 \par\pard\ql\li6230\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li1130\sb0\sl-466\slmult0 \par\pard\ql\li1130\ri848\sb58\sl-466
\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf1\f2\fs27 15gm of Pot s
ulphate, 0.5gm of copper sulphate and 25-40ml of Sulphuric acid. Heat \line \up0
\expndtw-1\charscalex100 the flask gently in an inclined position until froathi
ng ceases then boil briskly for 2 \line \up0 \expndtw-1\charscalex100 hours. All
ow to cool. Add approx 200ml of water and 25ml of Sod thiosulphate \line \up0 \e
xpndtw-2\charscalex100 solution (80gm/l) and mix. Add a piece of granulated Zinc
or anti bump granules and \line \up0 \expndtw-2\charscalex100 carefully pour do
wn the side of the flask sufficient Sodium Hydroxide sol (1+1) to \line \up0 \ex
pndtw-3\charscalex100 make the contents strongly alkaline (about 110ml). Before
mixing the acid and alkaline \up0 \expndtw-2\charscalex100 layers connect the fl
ask to a distillation apparatus incorporating an efficient splash head \up0 \exp
ndtw-3\charscalex100 and condenser. To the condenser fit a delivery tube which d
ips just below the surface of \up0 \expndtw-2\charscalex100 a pipetted vol of th
e digestion flask and boil until about 150ml of the distillate has been \up0 \ex
pndtw-2\charscalex100 collected. Add 5 drops of methyl red indicator and titrate
with 0.1N NaOH. Carry out a \up0 \expndtw-3\charscalex100 blank, lml of 0.1 HCl
or H\ul0\sub\cf17\f18\fs26 2\ul0\nosupersub\cf1\f2\fs27 SO\ul0\sub\cf17\f18\fs2
6 4\ul0\nosupersub\cf1\f2\fs27 is equivalent to 0.0014 of N. \par\pard\ql \li11
30\sb0\sl-310\slmult0 \par\pard\ql\li1130\sb0\sl-310\slmult0 \par\pard\ql\li1130
\sb23\sl-310\slmult0 \up0 \expndtw-4\charscalex100 Total protein is equal to N X
6.25. \par\pard\qj \li1850\sb0\sl-460\slmult0 \par\pard\qj\li1850\sb0\sl-460\sl
mult0 \par\pard\qj\li1850\sb0\sl-460\slmult0 \par\pard\qj\li1850\ri1571\sb67\sl460\slmult0 \up0 \expndtw-4\charscalex100 (Ref:- A.O.A.C 17\ul0\super\cf17\f18\f
s26 th\ul0\nosupersub\cf1\f2\fs27 edition,2000, Official Method 928.08 Nitrogen
in Meat \up0 \expndtw-5\charscalex100 (Alternative II)) \par\pard\qj \li1850\ri
1842\sb464\sl-480\slmult0 \up0 \expndtw0\charscalex104 PI also see I.S-5960 (Par
t 1) 1996 / I.S.O 937-1978 Meat and Meat \up0 \expndtw-4\charscalex100 Products
- determination of Nitrogen Content \par\pard\ql \li5867\sb0\sl-218\slmult0 \par
\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\p
ard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\par
d\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\
ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql
\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\l
i5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5
867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb165\sl-218\slmult0 \up0 \expndtw-3\
charscalex100 \ul0\nosupersub\cf10\f11\fs19 23
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g26}{\bkmkend Pg26}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
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l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li3696\sb0\sl-402\slmult0 \par\pard\ql\li3696\sb0\sl-402\slmult
0 \par\pard\ql\li3696\sb92\sl-402\slmult0 \up0 \expndtw0\charscalex102 \ul0\nosu
persub\cf5\f6\fs35 FISH AND FISH PRODUCTS \par\pard\ql \li1430\sb0\sl-356\slmult
0 \par\pard\ql\li1430\sb0\sl-356\slmult0 \par\pard\ql\li1430\sb0\sl-356\slmult0
\par\pard\ql\li1430\sb8\sl-356\slmult0 \up0 \expndtw0\charscalex92 \ul0\nosupers
ub\cf7\f8\fs31 1.0 FROZEN FISH \par\pard\ql \li2111\sb0\sl-310\slmult0 \par\pard
\ql\li2111\sb0\sl-310\slmult0 \par\pard\ql\li2111\sb82\sl-310\slmult0 \up0 \expn
dtw0\charscalex102 \ul0\nosupersub\cf1\f2\fs27 Fish stored in ice spoils as a re
sult of bacterial and enzyme action \par\pard\ql \li2111\sb150\sl-310\slmult0 \u
p0 \expndtw-4\charscalex100 which results in the formation of volatile bases, in
particular \par\pard\ql \li2111\sb170\sl-310\slmult0 \up0 \expndtw-5\charscalex
100 trimethylamine (TMA), dimethylamine (DMA) and ammonia. \par\pard\ql \li2111\
ri1470\sb22\sl-466\slmult0 \up0 \expndtw-4\charscalex100 Trimethylamine is the r
eduction product of Trimethylamine oxide during \line \up0 \expndtw-3\charscalex
100 spoilage while ammonia is formed mainly as the end product of protein \line
\up0 \expndtw-4\charscalex100 breakdown. The amounts of trimethylamine(TMA) and
total volatile \line \up0 \expndtw-3\charscalex100 nitrogen (TVN) present in fis
h are commonly used as indices of spoilage. \par\pard\ql \li1430\sb0\sl-379\slmu
lt0 \par\pard\ql\li1430\sb207\sl-379\slmult0 \up0 \expndtw0\charscalex91 \ul0\no
supersub\cf12\f13\fs33 1.1 Preparation of Sample \par\pard\ql \li1430\sb0\sl-310
\slmult0 \par\pard\ql\li1430\sb0\sl-310\slmult0 \par\pard\ql\li1430\sb98\sl-310\
slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.1.1 Frozen
Fish \par\pard\ql \li2111\sb0\sl-470\slmult0 \par\pard\ql\li2111\ri1620\sb8\sl470\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Place froz
en fish in a plastic bag and thaw by immersion in cold water at \up0 \expndtw-3\
charscalex100 a temperature not exceeding 4 C. Judge the completion of thawing by
\up0 \expndtw-4\charscalex100 gently squeezing the bag until no core or ice cry
stals can be felt. \line \up0 \expndtw-2\charscalex100 Transfer to a blender or
homogenizer to mince the sample. Ensure that \up0 \expndtw-3\charscalex100 the p
roduct remains at 4 C by pre cooling the blender. Analyse the \line \up0 \expndt
w-5\charscalex100 sample as quickly as possible. When storage is necessary keep
at a \line \up0 \expndtw-5\charscalex100 temperature not exceeding 4\ul0\super\c
f17\f18\fs26 0\ul0\nosupersub\cf1\f2\fs27 C \par\pard\ql \li5867\sb0\sl-218\slmu
lt0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult

0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0
\par\pard\ql\li5867\sb189\sl-218\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosu
persub\cf10\f11\fs19 24
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{\shp {\*\shpinst\shpleft1401\shptop2509\shpright10550\shpbottom2910\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1401\shptop2910\shpright10550\shpbottom4336\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft2083\shptop4914\shpright10550\shpbottom5380\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft2083\shptop5380\shpright10550\shpbottom5848\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft2083\shptop5848\shpright10550\shpbottom6313\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft2083\shptop6313\shpright10550\shpbottom6781\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1401\shptop8766\shpright10550\shpbottom9148\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1401\shptop9148\shpright10550\shpbottom10168\shpfhdr0\s
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{\shp {\*\shpinst\shpleft2083\shptop10664\shpright10195\shpbottom11130\shpfhdr0\
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{\shp {\*\shpinst\shpleft2083\shptop11598\shpright10195\shpbottom12066\shpfhdr0\
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{\shp {\*\shpinst\shpleft2083\shptop13000\shpright10195\shpbottom13468\shpfhdr0\
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{\shp {\*\shpinst\shpleft2083\shptop13468\shpright10195\shpbottom13933\shpfhdr0\
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g27}{\bkmkend Pg27}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
b0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0
\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\s
l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li2111\sb0\sl-310\slmult0 \par\pard\ql\li2111\sb202\sl-310\slmu
lt0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 (Ref:- Pearsons Co
mposition and Analysis of Foods 9\ul0\super\cf17\f18\fs26 th\ul0\nosupersub\cf1\
f2\fs27 edn,1991, page509) \par\pard\ql \li1411\sb0\sl-356\slmult0 \par\pard\ql

\li1411\sb0\sl-356\slmult0 \par\pard\ql\li1411\sb120\sl-356\slmult0 \up0 \expndt

w0\charscalex95 \ul0\nosupersub\cf7\f8\fs31 1.2 Determination of Total Volatile
Bases \par\pard\ql \li1411\sb0\sl-310\slmult0 \par\pard\ql\li1411\sb0\sl-310\slm
ult0 \par\pard\ql\li1411\sb22\sl-310\slmult0 \up0 \expndtw-7\charscalex100 \ul0\
nosupersub\cf14\f15\fs27 1.2.1 Principle \par\pard\qj \li2102\sb0\sl-470\slmult0
\par\pard\qj\li2102\ri1765\sb28\sl-470\slmult0 \up0 \expndtw-3\charscalex100 \u
l0\nosupersub\cf1\f2\fs27 The method is based on a semi micro distillation proce
dure. Extracts or \line \up0 \expndtw-4\charscalex100 solutions are made alkalin
e with sodium hydroxide and bases are steam \line \up0 \expndtw-5\charscalex100
distilled into standard acid and back titrated with standard alkali. \par\pard\q
l \li1411\sb0\sl-310\slmult0 \par\pard\ql\li1411\sb293\sl-310\slmult0 \up0 \expn
dtw-5\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.2.2 Apparatus \par\pard\ql \
li2102\sb0\sl-310\slmult0 \par\pard\ql\li2102\sb300\sl-310\slmult0 \up0 \expndtw
-5\charscalex100 \ul0\nosupersub\cf1\f2\fs27 (1)\ul0\nosupersub\cf15\f16\fs27 \
ul0\nosupersub\cf1\f2\fs27 Blender \par\pard\ql \li2102\sb170\sl-310\slmult0 \up
0 \expndtw-4\charscalex100 (2)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1
\f2\fs27 Semi micro distillation apparatus \par\pard\ql \li2102\sb150\sl-310\slm
ult0 \up0 \expndtw-5\charscalex100 (3)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupe
rsub\cf1\f2\fs27 Burette, Pipette, Conical flask \par\pard\ql \li1411\sb0\sl-310
\slmult0 \par\pard\ql\li1411\sb0\sl-310\slmult0 \par\pard\ql\li1411\sb10\sl-310\
slmult0 \up0 \expndtw-6\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.2.3 Reagen
ts \par\pard\ql \li2107\sb0\sl-310\slmult0 \par\pard\ql\li2107\sb0\sl-310\slmult
0 \par\pard\ql\li2107\sb10\sl-310\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nos
upersub\cf1\f2\fs27 (l)Trichloro acetic acid - 5 % \par\pard\ql \li2107\sb150\sl
-310\slmult0 \up0 \expndtw-5\charscalex100 (2) Sodium Hydroxide - 2 N \par\pard\
ql \li2107\sb170\sl-310\slmult0 \up0 \expndtw-5\charscalex100 (3)Hydrochloric ac
id - 0.01N \par\pard\ql \li2102\sb150\sl-310\slmult0 \up0 \expndtw-3\charscalex1
00 (4)Rosolic acid indicator - 1 % in 10 % ethanol (v /v) \par\pard\ql \li2102\s
b170\sl-310\slmult0 \up0 \expndtw-2\charscalex100 (5)Sodium Hydroxide - 0.01N \p
ar\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \pa
r\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\
pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pa
rd\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard
\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\q
l\li5867\sb26\sl-218\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf10\
f11\fs19 25
{\shp {\*\shpinst\shpleft1668\shptop2128\shpright10550\shpbottom2440\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8882}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(8882,312);(8882,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1668\shptop2440\shpright10550\shpbottom3258\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz82\shplid1
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8882}}{\sp{\sn geoBottom}{\sv 818}}
{\sp{\sn pVerticies}{\sv 8;4;(0,818);(8882,818);(8882,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1382\shptop3258\shpright10550\shpbottom3616\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz90\shplid2
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9168}}{\sp{\sn geoBottom}{\sv 358}}
{\sp{\sn pVerticies}{\sv 8;4;(0,358);(9168,358);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1382\shptop3616\shpright10550\shpbottom4580\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz106\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9168}}{\sp{\sn geoBottom}{\sv 964}}
{\sp{\sn pVerticies}{\sv 8;4;(0,964);(9168,964);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2073\shptop5075\shpright10550\shpbottom5543\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz130\shplid4
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8477}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8477,468);(8477,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2073\shptop5543\shpright10550\shpbottom6011\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz141\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8477}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8477,468);(8477,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2073\shptop6011\shpright10550\shpbottom6476\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz155\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8477}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(8477,465);(8477,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1382\shptop7076\shpright10550\shpbottom7388\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz178\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9168}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9168,312);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2073\shptop7873\shpright10550\shpbottom8346\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz202\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8477}}{\sp{\sn geoBottom}{\sv 473}}
{\sp{\sn pVerticies}{\sv 8;4;(0,473);(8477,473);(8477,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2073\shptop8346\shpright10550\shpbottom8819\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz233\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,473);(8477,473);(8477,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2073\shptop8819\shpright10550\shpbottom9289\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz263\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,470);(8477,470);(8477,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1382\shptop9880\shpright10550\shpbottom10192\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz293\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9168}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9168,312);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft2078\shptop10686\shpright10550\shpbottom11154\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz317\shplid12
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8472,468);(8472,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2078\shptop11154\shpright10550\shpbottom11622\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8472,468);(8472,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2078\shptop11622\shpright10550\shpbottom12097\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz372\shplid14
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,475);(8472,475);(8472,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2073\shptop12097\shpright10550\shpbottom12565\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft2073\shptop12565\shpright10550\shpbottom13033\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz428\shplid16
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8477,468);(8477,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft1382\shptop13631\shpright10550\shpbottom13943\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz456\shplid17
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9168,312);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840{\shp
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{\sp{\sn fBehindDocument}{\sv 1}}
{\sp{\sn pib}{\sv {\pict\jpegblip
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14514005145140051451400514514005145140051451401fffd9}}}}}\pard\sb0\sl-240{\bkmks
tart Pg28}{\bkmkend Pg28}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li
5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li59
49\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949
\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\char
scalex100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH
PRODUCTS \par\pard\ql \li1411\sb0\sl-310\slmult0 \par\pard\ql\li1411\sb202\sl-31
0\slmult0 \up0 \expndtw-6\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.2.4 Proc
edure \par\pard\ql \li2786\sb0\sl-310\slmult0 \par\pard\ql\li2786\sb300\sl-310\s
lmult0\tx4276 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Weigh 10
0 \tab \up0 \expndtw-4\charscalex100 0.5 gm prepared sample into a homogenizer w
ith 300 ml \par\pard\ql \li2111\ri1181\sb17\sl-472\slmult0 \up0 \expndtw-4\chars
calex100 of Trichloracetic acid. Run the homogenizer to obtain a uniform\ul0\nos
upersub\cf10\f11\fs19 \ul0\nosupersub\cf1\f2\fs27 slurry. Filter \up0 \expndtw3\charscalex100 or centrifuge to obtain a clear extract. Pipette 5 ml of the ext
ract into a semi \up0 \expndtw-2\charscalex100 micro distillation apparatus. Add
5 ml of 2 N NaOH. Steam distill. Collect \up0 \expndtw-1\charscalex100 distilla

te in 15 ml of 0.01 N standard hydrochloric acid. Add indicator \line \up0 \expn

dtw-1\charscalex100 (Rosolic acid). Titrate the liberated acid to a pale pink en
d point with 0.01 \up0 \expndtw-2\charscalex100 N sodium hydroxide. Do a blank d
etermination. \par\pard\ql \li1411\sb0\sl-310\slmult0 \par\pard\ql\li1411\sb272\
sl-310\slmult0 \up0 \expndtw-6\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.2.5
Calculation \par\pard\ql \li2136\sb310\sl-310\slmult0 \up0 \expndtw-4\charscale
x100 \ul0\nosupersub\cf1\f2\fs27 TVBN (mg / 100 gm) = (N) 14 (300 + W) x V \ul0\
sub\cf17\f18\fs26 1 \par\pard\ql \li6266\sb0\sl-299\slmult0 \up0 \expndtw-9\char
scalex100 \ul0\nosupersub\cf1\f2\fs27 500 \par\pard\ql \li2136\sb0\sl-310\slmult
0 \par\pard\ql\li2136\sb2\sl-310\slmult0 \up0 \expndtw-4\charscalex100 Where \pa
r\pard\qj \li2841\ri4074\sb147\sl-580\slmult0 \up0 \expndtw-2\charscalex100 V\ul
0\nosupersub\cf17\f18\fs18 I\ul0\super\cf1\f2\fs40 = Volume of standard acid co
nsumed \line \up0 \expndtw-6\charscalex100 W = water content of sample (gm / 100
gm) \par\pard\ql \li2042\sb0\sl-310\slmult0 \par\pard\ql\li2042\sb0\sl-310\slmu
lt0 \par\pard\ql\li2042\sb124\sl-310\slmult0 \up0 \expndtw-3\charscalex100 (Ref:
- Pearson\u8217?s Composition and Analysis of Foods 9\ul0\super\cf17\f18\fs26 th
\ul0\nosupersub\cf1\f2\fs27 edn,1991, page510) \par\pard\ql \li1435\sb0\sl-310\
slmult0 \par\pard\ql\li1435\sb0\sl-310\slmult0 \par\pard\ql\li1435\sb0\sl-310\sl
mult0 \par\pard\ql\li1435\sb160\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul
0\nosupersub\cf14\f15\fs27 1.3 Determination of Histamine \par\pard\ql \li1411\s
b0\sl-310\slmult0 \par\pard\ql\li1411\sb0\sl-310\slmult0 \par\pard\ql\li1411\sb1
0\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf1\f2\fs27 The p
resence of Histamine is also an indicator of decomposition and has been \par\par
d\qj \li1411\ri1362\sb10\sl-480\slmult0 \up0 \expndtw-2\charscalex100 linked to
scomboid poisoning. The natural level in fresh fish is less than 5 mg per \line
\up0 \expndtw-4\charscalex100 100 gm and the higher values in decomposing fish a
re due to decarboxylation of \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\q
l\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\
li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb45\sl-218\slmult0 \up0 \expndtw3\charscalex100 \ul0\nosupersub\cf10\f11\fs19 26 \par\pard\sect\sectd\fs24\paper
w11900\paperh16840\pard\sb0\sl-240{\bkmkstart Pg29}{\bkmkend Pg29}\par\pard\ql \
li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li
5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li59
49\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949
\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf9\f10\fs16
MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUCTS \par\pard\qj \li1411\sb0\sl-46
0\slmult0 \par\pard\qj\li1411\ri1626\sb68\sl-460\slmult0 \up0 \expndtw-2\charsca
lex100 \ul0\nosupersub\cf1\f2\fs27 histidine. Histamine is produced during the s
torage of pelagic species e.g tuna, \up0 \expndtw-3\charscalex100 mackerels, car
angidaie species at elevated temperatures. \par\pard\ql \li1425\sb0\sl-310\slmul
t0 \par\pard\ql\li1425\sb14\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\no
supersub\cf14\f15\fs27 1.3.1 Principle \par\pard\ql \li1411\sb0\sl-310\slmult0 \
par\pard\ql\li1411\sb300\sl-310\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosup
ersub\cf1\f2\fs27 Bacterial enzyme decarboxylase free histidine in the muscle to
histamine \par\pard\ql \li1411\sb170\sl-310\slmult0 \up0 \expndtw-2\charscalex1
00 The concentration of histamine is an indicator of bacterial spoilage. Free \p
ar\pard\qj \li1411\ri1172\sb10\sl-480\slmult0 \up0 \expndtw-2\charscalex100 hist
amine is extracted from fish with methanol. The extract is chromatographed on \u
p0 \expndtw-3\charscalex100 silica gel plates. Histamine is visualized with ninh
ydrin \par\pard\ql \li1425\sb0\sl-310\slmult0 \par\pard\ql\li1425\sb291\sl-310\s
lmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.3.2 Apparat
us \par\pard\ql \li2121\sb0\sl-310\slmult0 \par\pard\ql\li2121\sb300\sl-310\slmu
lt0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf1\f2\fs27 (1)\ul0\nosupersub
\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Chromatographic tank \par\pard\ql \l
i2121\sb170\sl-310\slmult0 \up0 \expndtw-4\charscalex100 (2)\ul0\nosupersub\cf15
\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Silica gel thin layer (TLC) plates or rea
dy coated plates \par\pard\ql \li1411\sb0\sl-310\slmult0 \par\pard\ql\li1411\sb3
00\sl-310\slmult0 \up0 \expndtw-6\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.
3.3Reagents \par\pard\qj \li2116\ri2293\sb0\sl-480\slmult0 \up0 \expndtw-5\chars
calex100 \ul0\nosupersub\cf1\f2\fs27 (1)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosu

persub\cf1\f2\fs27 Histamine standard (0.2 mg / ml) - Dissolve 16.4 mg histamine

\up0 \expndtw-5\charscalex100 dihydrochloride in 50 ml methanol \par\pard\ql \l
i2116\sb111\sl-310\slmult0 \up0 \expndtw-1\charscalex100 (2)\ul0\nosupersub\cf15
\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Solvent system - Methanol: Cone ammonia (
95 : 5) \par\pard\qj \li2116\ri2258\sb47\sl-460\slmult0 \up0 \expndtw-4\charscal
ex100 (3)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Ninhydrin sp
ray reagent - Dissolve 0.3 gm ninhydrin in 100 ml \up0 \expndtw-4\charscalex100
n - butanol and add 3 ml glacial acetic acid \par\pard\ql \li1425\sb0\sl-310\slm
ult0 \par\pard\ql\li1425\sb294\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0
\nosupersub\cf14\f15\fs27 1.3.4 Procedure \par\pard\ql \li1411\sb0\sl-470\slmult
0 \par\pard\ql\li1411\ri1256\sb28\sl-470\slmult0 \up0 \expndtw-2\charscalex100 \
ul0\nosupersub\cf1\f2\fs27 Homogenise 10 gm fish with 50 ml methanol and transfe
r with methanol rinsings \line \up0 \expndtw-3\charscalex100 to a 100 ml volumet
ric flask. Immerse stopppered flask in a water bath at 60 C \line \up0 \expndtw3\charscalex100 for 15 minutes. Cool, make upto 100 ml with methanol and centrif
uge a portion to \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0
\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb105
\sl-218\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf10\f11\fs19 27
{\shp {\*\shpinst\shpleft1382\shptop2128\shpright10550\shpbottom2596\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9168,468);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1382\shptop2596\shpright10550\shpbottom3061\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz49\shplid1
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9168,465);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1396\shptop3061\shpright10550\shpbottom3376\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz67\shplid2
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,315);(9154,315);(9154,0);(0,0)}}
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{\shp {\*\shpinst\shpleft1396\shptop3376\shpright10550\shpbottom3688\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz76\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9154,312);(9154,0);(0,0)}}

{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}

}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1382\shptop4175\shpright10550\shpbottom4645\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz102\shplid4
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,470);(9168,470);(9168,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1382\shptop4645\shpright10550\shpbottom5118\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz133\shplid5
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}}
{\shp {\*\shpinst\shpleft1382\shptop5118\shpright10550\shpbottom5588\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz143\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,470);(9168,470);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1382\shptop5588\shpright10550\shpbottom6064\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz157\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,476);(9168,476);(9168,0);(0,0)}}
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{\shp {\*\shpinst\shpleft1396\shptop6652\shpright10550\shpbottom6966\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz172\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9154,314);(9154,0);(0,0)}}

{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}

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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft2092\shptop7588\shpright10550\shpbottom7900\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz196\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,312);(8458,312);(8458,0);(0,0)}}
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{\shp {\*\shpinst\shpleft2092\shptop7900\shpright10550\shpbottom8368\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz227\shplid10
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{\shp {\*\shpinst\shpleft1382\shptop8521\shpright10550\shpbottom8833\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1382\shptop8833\shpright10550\shpbottom9301\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft2088\shptop9301\shpright10550\shpbottom9769\shpfhdr0\sh
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8462,468);(8462,0);(0,0)}}

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{\shp {\*\shpinst\shpleft2088\shptop9769\shpright10550\shpbottom10235\shpfhdr0\s
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{\shp {\*\shpinst\shpleft2088\shptop10703\shpright10550\shpbottom11176\shpfhdr0\
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{\shp {\*\shpinst\shpleft2088\shptop11176\shpright10550\shpbottom11641\shpfhdr0\
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{\shp {\*\shpinst\shpleft1382\shptop13988\shpright10550\shpbottom14454\shpfhdr0\
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g30}{\bkmkend Pg30}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
b0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0
\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\s
l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li1411\sb0\sl-310\slmult0 \par\pard\ql\li1411\sb0\sl-310\slmult
0 \par\pard\ql\li1411\sb32\sl-310\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nos
upersub\cf1\f2\fs27 produce clear extract for TLC \par\pard\ql \li1416\ri1222\sb
22\sl-465\slmult0\fi14 \up0 \expndtw-3\charscalex100 Spot extract and histamine
solution on TLC plate. A useful spotting regime is 1, 5, \up0 \expndtw-2\charsca
lex100 10 ul of extract and 0.5, 2, 5, and 10 ul of histamine solution (eqvt to
0.1 , 0.4 , 1 \up0 \expndtw-2\charscalex100 and 2 ug standard.). Develop plates
in the solvent mixture. Thoroughly dry the \up0 \expndtw-3\charscalex100 plate w
ith a hair dryer (residual ammonia will react with spray reagent) and spray \up0
\expndtw-2\charscalex100 with ninhydrin reagent. Dry and gently warm plate with
a hair dryer to accelerate \up0 \expndtw-1\charscalex100 colour development. Es
timate histamine level in the extract (ug / ul) by \line \up0 \expndtw-2\charsca
lex100 comparison of spot size and intensities with those of standards. Rerun pl

ate with \up0 \expndtw-3\charscalex100 different quantities of sample extract an

d standard if necessary. \par\pard\ql \li1430\sb0\sl-310\slmult0 \par\pard\ql\li
1430\sb0\sl-310\slmult0 \par\pard\ql\li1430\sb4\sl-310\slmult0 \up0 \expndtw-4\c
harscalex100 \ul0\nosupersub\cf14\f15\fs27 1.3.5 Calculation \par\pard\ql \li155
0\sb0\sl-310\slmult0 \par\pard\ql\li1550\sb300\sl-310\slmult0 \up0 \expndtw-2\ch
arscalex100 \ul0\nosupersub\cf1\f2\fs27 Histamine in fish (mg / 100 gm) = Histam
ine in extract ( ug / ul x 1000) \par\pard\qj \li1411\sb0\sl-460\slmult0 \par\pa
rd\qj\li1411\sb0\sl-460\slmult0 \par\pard\qj\li1411\ri2694\sb67\sl-460\slmult0 \
up0 \expndtw-3\charscalex100 (Ref:- Lieber, E.R and Taylor, S.L 1978, TLC screen
ing methods for \up0 \expndtw-3\charscalex100 histamine in tuna fish - J . Chrom
atgr 153 , 143 - 52) \par\pard\ql \li1430\sb0\sl-310\slmult0 \par\pard\ql\li1430
\sb0\sl-310\slmult0 \par\pard\ql\li1430\sb0\sl-310\slmult0 \par\pard\ql\li1430\s
b154\sl-310\slmult0 \up0 \expndtw-5\charscalex100 \ul0\nosupersub\cf14\f15\fs27
1.3.0 A Determination of Histamine - Alternate method \par\pard\ql \li1430\sb0\s
l-310\slmult0 \par\pard\ql\li1430\sb0\sl-310\slmult0 \par\pard\ql\li1430\sb0\sl310\slmult0 \par\pard\ql\li1430\sb260\sl-310\slmult0 \up0 \expndtw-2\charscalex1
00 1.3.0 A.I Reagents \par\pard\ql \li2131\sb0\sl-310\slmult0 \par\pard\ql\li213
1\sb300\sl-310\slmult0 \up0 \expndtw0\charscalex100 \ul0\nosupersub\cf1\f2\fs27
(a)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Benzene - n - buta
nol mixture -3 + 2 (v/v) \par\pard\ql \li2131\sb170\sl-310\slmult0 \up0 \expndtw
-5\charscalex100 (b)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 C
otton acid succinate - Dissolve 5 gm anhydrous sodium acetate \par\pard\ql \li58
67\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867
\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb192\sl-218\slmult0 \up0 \expndtw-2\cha
rscalex100 \ul0\nosupersub\cf10\f11\fs19 28
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g31}{\bkmkend Pg31}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
b0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0
\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\s
l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li2505\sb0\sl-472\slmult0 \par\pard\ql\li2505\ri1131\sb47\sl-47
2\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 fused just b
efore use, and 40 gm succinic anhydride in 300 ml acetic acid \up0 \expndtw-3\ch
arscalex100 in 500 Erlenmeyer flask. Immerse 10 gm absorbent cotton, cut into st
rips \up0 \expndtw-3\charscalex100 in solution, attach drying tube containing dr
ying agent and heat 48 hrs at \line \up0 \expndtw-4\charscalex100 100 \ul0\super

\cf17\f18\fs26 0\ul0\nosupersub\cf1\f2\fs27 C.( flask may be immersed to neck i

n active steam bath). Filter, \line \up0 \expndtw-3\charscalex100 wash well with
water, HCl (1+9), water and finally with alcohol. Dry in \line \up0 \expndtw-4\
charscalex100 vaccum at 100 \ul0\super\cf17\f18\fs26 0\ul0\nosupersub\cf1\f2\fs2
7 C \par\pard\ql \li2111\sb122\sl-310\slmult0 \up0 \expndtw-2\charscalex100 (c)
\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Diazonium reagent - D
issolve 0.1 gm p - nitroaniline \par\pard\qj \li2481\ri1895\sb38\sl-470\slmult0
\up0 \expndtw-5\charscalex100 recrystallised from hot water and dilute to 100 ml
with 0.1 N HCL. \line \up0 \expndtw-4\charscalex100 Store in refrigerator. Diss
olve 4 gm of NaNO\ul0\sub\cf17\f18\fs26 2\ul0\nosupersub\cf1\f2\fs27 in water a
nd dilute \line \up0 \expndtw-4\charscalex100 to 100 ml. Store in refrigerator.
Just before use place 10 ml p -\par\pard\qj \li2481\ri1950\sb0\sl-460\slmult0 \u
p0 \expndtw-5\charscalex100 nitroaniline solution in ice bath for 25 minutes, ad
d 1 ml of NaNo\ul0\sub\cf17\f18\fs26 2 \up0 \expndtw-5\charscalex100 \ul0\nosupe
rsub\cf1\f2\fs27 solution, mix and let stand in bath 5 minutes before use. \par\
pard\ql \li2111\ri2226\sb11\sl-470\slmult0\tx2481\tx2481 \up0 \expndtw-5\charsca
lex100 (d)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Coupling bu
ffer - Dissolve 7. 15 gm sodium metaborate and 5.7 \line\tab \up0 \expndtw-4\cha
rscalex100 gm sod.carbonate in water and dilute to 100 ml. Store in \line \tab \
up0 \expndtw-4\charscalex100 polyethylene bottle \par\pard\ql \li2111\ri1891\sb0
\sl-468\slmult0\tx2481\tx2481\tx2481\tx2481\tx2481 \up0 \expndtw-3\charscalex100
(e)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Barbital buffer Dissolve 10 gm of sodium barbital in 1 litre water \line\tab \up0 \expndtw-3\ch
arscalex100 and adjust to pH 7.7 with acetic acid ( 1+ 15) ( about 25 - 30 ml),
\line \tab \up0 \expndtw-4\charscalex100 using pH meter. Store in refrigerator t
o prevent mould growth. \line \tab \up0 \expndtw-4\charscalex100 Dissolve any pp
t by warming before use ( 50 - 250 ml bottle of the \line \tab \up0 \expndtw-3\c
harscalex100 buffer may be kept at room temperature and replenished from \line \
tab \up0 \expndtw-3\charscalex100 main supply when mould growth is apparent) \pa
r\pard\ql \li2111\ri2011\sb7\sl-465\slmult0\tx2481\tx2481\tx2481\tx2481 \up0 \ex
pndtw-3\charscalex100 (f)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\f
s27 Histamine standard solution - Dry Histamine dihydrochloride 2 \line\tab \up0
\expndtw-3\charscalex100 hrs over H\ul0\sub\cf17\f18\fs26 2\ul0\nosupersub\cf1\
f2\fs27 SO\ul0\sub\cf17\f18\fs26 4\ul0\nosupersub\cf1\f2\fs27 . Dissolve 0. 1656
gm dried histamine 2 HCl in \line \tab \up0 \expndtw-5\charscalex100 water and
dilute to 100 ml (1 ml = 1 mg histamine ). Dilute 10 ml \line \tab \up0 \expndtw
-4\charscalex100 of this stock solution to 100 ml with water ( 1 ml = 100 ug \li
ne \tab \up0 \expndtw-4\charscalex100 histamine. Dilute 5 ml of this dilute stan
dard solution and 5 ml of \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\l
i5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5
867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb28\sl-218\slmult0 \up0 \expndtw-2\c
harscalex100 \ul0\nosupersub\cf10\f11\fs19 29
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{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2083\shptop11521\shpright10550\shpbottom11987\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(8467,466);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop11987\shpright10550\shpbottom12460\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz501\shplid21
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 473}}
{\sp{\sn pVerticies}{\sv 8;4;(0,473);(8467,473);(8467,0);(0,0)}}

{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}

}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft2083\shptop12460\shpright10550\shpbottom12928\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz537\shplid22
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2083\shptop12928\shpright10550\shpbottom13393\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz577\shplid23
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(8467,465);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2083\shptop13393\shpright10550\shpbottom13861\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft2083\shptop13861\shpright10550\shpbottom14329\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz609\shplid25
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g32}{\bkmkend Pg32}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
b0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0
\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\s
l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\qj \li2481\sb0\sl-460\slmult0 \par\pard\qj\li2481\ri2167\sb68\sl-46

0\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 methanol to

100 ml with water ( 1 ml = 5 ug histamine. Store in \up0 \expndtw-3\charscalex10
0 cold. Prepare fresh standards weekly. \par\pard\qj \li2111\ri1410\sb16\sl-466\
slmult0 \up0 \expndtw-1\charscalex100 (g) 4 - methyl - 2 pentanone ( methyl isob
utyl ketone). To recover used \line \up0 \expndtw-4\charscalex100 ketone
wash
once with saturated sodium bicarbonate solution and 3 times \line \up0 \expndtw
-3\charscalex100 with water, distill retaining fraction boiling at 115-118 C and
check A at \line \up0 \expndtw-6\charscalex100 475 nm. \par\pard\ql \li2116\sb1
23\sl-310\slmult0 \up0 \expndtw-3\charscalex100 (h) Benzaldehyde - chlorine free
\par\pard\ql \li2116\sb170\sl-310\slmult0 \up0 \expndtw-2\charscalex100 (i) Dil
ute sulphuric acid - 0.01 M accurately standardized. \par\pard\ql \li1411\sb0\sl
-310\slmult0 \par\pard\ql\li1411\sb0\sl-310\slmult0 \par\pard\ql\li1411\sb10\sl310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 1.3.0A 2
Preparation of CAS column \par\pard\ql \li2176\sb0\sl-465\slmult0 \par\pard\ql\
li2176\ri1203\sb37\sl-465\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\
cf1\f2\fs27 Prepare column by firmly lacing small plug of cotton acid succinate
(CAS \up0 \expndtw-3\charscalex100 ca 50 mg) in column by cutting off or blowing
out bottom of 15 ml \line \up0 \expndtw-4\charscalex100 centrifuge tube. Wash p
lug with 15 ml portions of water and two 3 ml \line \up0 \expndtw-3\charscalex10
0 portions of alcohol. Let solvents drip through CAS syringing out column by \up
0 \expndtw-3\charscalex100 blowing out last portion of each solvent, using 10 ml
syringe with needle \line \up0 \expndtw-4\charscalex100 inserted through rubber
stopper. CAS plugs may be used for months by \line \up0 \expndtw-2\charscalex10
0 washing shortly after use with water and alcohol as above and protecting \line
\up0 \expndtw-3\charscalex100 from dust with inverted beaker \par\pard\ql \li14
20\sb0\sl-310\slmult0 \par\pard\ql\li1420\sb294\sl-310\slmult0 \up0 \expndtw-4\c
harscalex100 \ul0\nosupersub\cf14\f15\fs27 1.3.0A 3 Determination \par\pard\qj \
li2116\sb0\sl-466\slmult0 \par\pard\qj\li2116\ri1436\sb16\sl-466\slmult0 \up0 \e
xpndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Transfer 10 gm prepared sampl
e to semi micro container of high speed \line \up0 \expndtw-3\charscalex100 blen
der, add about 50 ml methanol and blend about 2 minutes. Transfer to \line \up0
\expndtw-2\charscalex100 100 ml glass stoppered volumetric flask. Rinsing lid an
d blender jar with \line \up0 \expndtw-2\charscalex100 methanol and adding rinsi
ngs to flask, Heat in water bath to 60 C and let \par\pard\ql \li5867\sb0\sl-218
\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\s
lmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb67\sl-218\sl
mult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf10\f11\fs19 30
{\shp {\*\shpinst\shpleft2083\shptop2128\shpright10550\shpbottom2596\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
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}}
{\shp {\*\shpinst\shpleft2083\shptop2596\shpright10550\shpbottom3061\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz53\shplid1
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(8467,465);(8467,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}

}}
{\shp {\*\shpinst\shpleft2083\shptop3061\shpright10550\shpbottom3534\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz71\shplid2
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,473);(8467,473);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop3534\shpright10550\shpbottom4002\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz101\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop4002\shpright10550\shpbottom4468\shpfhdr0\sh
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}}
{\shp {\*\shpinst\shpleft2083\shptop4468\shpright10550\shpbottom4936\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2088\shptop4940\shpright10550\shpbottom5408\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz156\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8462}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8462,468);(8462,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}

}}
{\shp {\*\shpinst\shpleft2088\shptop5408\shpright10550\shpbottom5874\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz184\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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}}
{\shp {\*\shpinst\shpleft1382\shptop6476\shpright10550\shpbottom6791\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz208\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,315);(9168,315);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2150\shptop7285\shpright10550\shpbottom7753\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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}}
{\shp {\*\shpinst\shpleft2150\shptop7753\shpright10550\shpbottom8219\shpfhdr0\sh
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}}
{\shp {\*\shpinst\shpleft2150\shptop8219\shpright10550\shpbottom8687\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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}}
{\shp {\*\shpinst\shpleft2150\shptop8687\shpright10550\shpbottom9155\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft2150\shptop9620\shpright10550\shpbottom10088\shpfhdr0\s
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2150\shptop10088\shpright10550\shpbottom10556\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8400,468);(8400,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2150\shptop10556\shpright10550\shpbottom11022\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,466);(8400,466);(8400,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}

}}
{\shp {\*\shpinst\shpleft1392\shptop11627\shpright10550\shpbottom11939\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft5858\shptop11939\shpright10550\shpbottom11963\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz342\shplid18
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,24);(4692,24);(4692,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2088\shptop12416\shpright10550\shpbottom12882\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2088\shptop12882\shpright10550\shpbottom13350\shpfhdr0\
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8462,468);(8462,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft2088\shptop13350\shpright10550\shpbottom13818\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8462,468);(8462,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft2088\shptop13818\shpright10550\shpbottom14284\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz407\shplid22
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,466);(8462,466);(8462,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g33}{\bkmkend Pg33}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
b0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0
\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\s
l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li2116\sb0\sl-466\slmult0 \par\pard\ql\li2116\ri1371\sb58\sl-46
6\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 stand 15 min
utes at this temperature. Cool to 25 \ul0\super\cf17\f18\fs26 0\ul0\nosupersub\c
f1\f2\fs27 C , dilute to volume with \up0 \expndtw-3\charscalex100 methanol and
filter through folded filter paper. Alcohol filterate may be \up0 \expndtw-4\ch
arscalex100 stored in refrigerator for several weeks. (Light powdery ppt separat
ing on \up0 \expndtw-5\charscalex100 storage may be ignored) \par\pard\ql \li211
1\ri1150\sb330\sl-472\slmult0 \up0 \expndtw-2\charscalex100 Dilute 5 ml of filtr
ate to 100 ml with water (disregard turbidity). Pipette 5 ml \line \up0 \expndtw
-3\charscalex100 aliquot into 16 x 150 mm glass stoppered test tube and add 1 dr
op \line \up0 \expndtw-2\charscalex100 benzaldehyde and 0.2 ml 20 % (w/v) NaOH (
pH after adding alkali should \line \up0 \expndtw-3\charscalex100 be about 12.4
- 12.5). Shake vigorously about 25 times. Let stand 5 minutes \line \up0 \expnd
tw-2\charscalex100 and add 5 ml benzene -n - butanol mixture. Shake vigorously a
bout 25 \line \up0 \expndtw-1\charscalex100 times and let stand 5 minutes to sep
arate. If emulsion forms centrifuge. \par\pard\ql \li2111\sb0\sl-310\slmult0 \pa
r\pard\ql\li2111\sb292\sl-310\slmult0 \up0 \expndtw-2\charscalex100 Transfer upp
er layer with fine tipped tube equipped with rubber bulb to \par\pard\ql \li2111
\sb170\sl-310\slmult0 \up0 \expndtw-3\charscalex100 previously prepared CAS colu
mn, avoiding transfer of any aqueous phase. \par\pard\ql \li2111\ri1141\sb18\sl470\slmult0 \up0 \expndtw-2\charscalex100 Re extract aqueous solution with 5 ml
of benzene butanol mixture as before, \up0 \expndtw-2\charscalex100 shaking , le
tting stand 5 minutes and transferring upper layer to column. \line \up0 \expndt
w-2\charscalex100 Rinse lip and sides of column with fine stream of alcohol from
wash bottle \up0 \expndtw-3\charscalex100 syringing out CAS. Wash column with t
wo 3 ml portions water and syringe \up0 \expndtw-4\charscalex100 out. Discard so
lvents and washes. \par\pard\ql \li2111\sb0\sl-310\slmult0 \par\pard\ql\li2111\s
b0\sl-310\slmult0 \par\pard\ql\li2111\sb123\sl-310\slmult0 \up0 \expndtw-4\chars
calex100 Elute histamine from CAS into 25 ml glass stoppered Erlenmeyer by \par\
pard\ql \li2111\sb150\sl-310\slmult0 \up0 \expndtw-3\charscalex100 washing down
sides of tube with 2 ml 0.01M H\ul0\sub\cf17\f18\fs26 2\ul0\nosupersub\cf1\f2\fs
27 SO\ul0\sub\cf17\f18\fs26 4\ul0\nosupersub\cf1\f2\fs27 ( volume and \par\pard
\qj \li2111\ri1355\sb10\sl-480\slmult0 \up0 \expndtw-3\charscalex100 concentrati
on of acid are critical) followed by 3 ml water. Syringe out after \up0 \expndtw
-4\charscalex100 dripping ceases. \par\pard\ql \li2111\sb0\sl-310\slmult0 \par\p
ard\ql\li2111\sb291\sl-310\slmult0 \up0 \expndtw-3\charscalex100 Cool eluate in
ice bath, weighting flask with clamp to prevent tipping and let \par\pard\ql \li
5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li58
67\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867
\sb34\sl-218\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf10\f11\fs19
31

{\shp {\*\shpinst\shpleft2088\shptop2128\shpright10550\shpbottom2596\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8462,468);(8462,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2088\shptop2596\shpright10550\shpbottom3061\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2088\shptop3061\shpright10550\shpbottom3529\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2088\shptop3529\shpright10550\shpbottom3997\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop4468\shpright10550\shpbottom4938\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft2083\shptop4938\shpright10550\shpbottom5411\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,473);(8467,473);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop5411\shpright10550\shpbottom5879\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz141\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop5879\shpright10550\shpbottom6349\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop6349\shpright10550\shpbottom6817\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop6817\shpright10550\shpbottom7288\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,471);(8467,471);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft2083\shptop7288\shpright10550\shpbottom8236\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz230\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,948);(8467,948);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop8236\shpright10550\shpbottom8706\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,470);(8467,470);(8467,0);(0,0)}}
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{\shp {\*\shpinst\shpleft2083\shptop8706\shpright10550\shpbottom9176\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,470);(8467,470);(8467,0);(0,0)}}
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{\shp {\*\shpinst\shpleft2083\shptop10117\shpright10550\shpbottom10585\shpfhdr0\
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{\shp {\*\shpinst\shpleft2083\shptop12930\shpright10550\shpbottom13396\shpfhdr0\
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{\shp {\*\shpinst\shpleft2083\shptop13864\shpright10466\shpbottom14336\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g34}{\bkmkend Pg34}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
b0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0
\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\s
l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\qj \li2111\sb0\sl-460\slmult0 \par\pard\qj\li2111\ri1320\sb68\sl-46
0\slmult0 \up0 \expndtw0\charscalex100 \ul0\nosupersub\cf1\f2\fs27 stand 5-10 mi
nutes. Add 0.5 ml of cooled diazonium reagent and let stand \up0 \expndtw-3\char
scalex100 5 minutes in ice bath. Add 0.5 ml coupling buffer \par\pard\ql \li2111
\ri1140\sb14\sl-468\slmult0 \up0 \expndtw-3\charscalex100 (volume is critical, o
stwald pipette is convenient) with continuous shaking or \line \up0 \expndtw-3\c
harscalex100 swirling to avoid localized alkalinity ( pH after addition of coupl
ing buffer \line \up0 \expndtw-2\charscalex100 5-6). Let stand 5 minutes in ice
bath, saturated solution with about 0.25 gm \line \up0 \expndtw-3\charscalex100
powdered Na\ul0\sub\cf17\f18\fs26 2\ul0\nosupersub\cf1\f2\fs27 B\ul0\sub\cf17\f1
8\fs26 4\ul0\nosupersub\cf1\f2\fs27 O\ul0\sub\cf17\f18\fs26 7\ul0\nosupersub\cf1
\f2\fs27 , 10 H\ul0\sub\cf17\f18\fs26 2\ul0\nosupersub\cf1\f2\fs27 O added in on
e portion. Shake solution \line \up0 \expndtw-4\charscalex100 immediately and co
ntinuously about 30 seconds to ensure rapid and \line \up0 \expndtw-3\charscalex
100 complete saturation (final pH about 8.6). Let stand 15 minutes in ice bath.
\par\pard\ql \li2111\sb0\sl-310\slmult0 \par\pard\ql\li2111\sb293\sl-310\slmult0
\up0 \expndtw-5\charscalex100 Pipette in 5 ml methyl isobutyl ketone and shake
vigorously 25 times. \par\pard\ql \li2111\ri1158\sb18\sl-470\slmult0 \up0 \expnd
tw-2\charscalex100 Immediately transfer both layers to 16 x 150 mm test tube and
let stand 10 \line \up0 \expndtw-3\charscalex100 minutes at room temperature to
separate and warm up. Transfer upper layer \line \up0 \expndtw-3\charscalex100
with fine tip dropper to second 18 x 150 mm glass stoppered test tube \line \up0
\expndtw-2\charscalex100 containing 5 ml barbital buffer. Avoid transferring aq
ueous and solid phases \up0 \expndtw-2\charscalex100 if present (transfer need n
ot be quantitative). Shake vigorously about 25 \line \up0 \expndtw-3\charscalex1
00 times ( pH of barbital buffer after washing about 8.3- 8.4). Let stand 10 \li
ne \up0 \expndtw-4\charscalex100 minutes to separate. \par\pard\ql \li2111\sb0\s
l-310\slmult0 \par\pard\ql\li2111\sb273\sl-310\slmult0 \up0 \expndtw-4\charscale
x100 Transfer upper layer with fine tip dropper to 1 cm cell and determine A at
\par\pard\ql \li2111\sb170\sl-310\slmult0 \up0 \expndtw-5\charscalex100 475 nm a
gainst methyl isobutyl ketone. Repeat determination on samples \par\pard\qj \li2
111\ri1193\sb18\sl-470\slmult0 \up0 \expndtw-2\charscalex100 yielding A values >

25 ug standard by diluting 1 ml methanol filtrate to 100 \up0 \expndtw-2\charsc

alex100 ml with water. Alternatively, aqueous solutions may be diluted 1+ 4 or m
ore \up0 \expndtw-3\charscalex100 with water. \par\pard\qj \li2111\sb0\sl-460\sl
mult0 \par\pard\qj\li2111\ri1193\sb19\sl-460\slmult0 \up0 \expndtw-3\charscalex1
00 Conduct standard and blank determinations as follows. Pipette 5 ml of 5 ug /
\line \up0 \expndtw-3\charscalex100 ml histamine standard solution into 16 x 150
mm glass stoppered test tube. \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard
\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\q
l\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb48\sl-218\slmult0 \up0 \expndt
w-2\charscalex100 \ul0\nosupersub\cf10\f11\fs19 32
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{\shp {\*\shpinst\shpleft2083\shptop11968\shpright10550\shpbottom12433\shpfhdr0\

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{\shp {\*\shpinst\shpleft2083\shptop12901\shpright10550\shpbottom13840\shpfhdr0\
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{\shp {\*\shpinst\shpleft2083\shptop13840\shpright10550\shpbottom14308\shpfhdr0\
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15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g35}{\bkmkend Pg35}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
b0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0
\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\s
l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li2111\sb0\sl-310\slmult0 \par\pard\ql\li2111\sb0\sl-310\slmult
0 \par\pard\ql\li2111\sb32\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nos
upersub\cf1\f2\fs27 Pipette 5 ml of 5% methanol into a similar tube for blank. A
dd 1 drop \par\pard\ql \li2111\ri1497\sb22\sl-465\slmult0 \up0 \expndtw-4\charsc
alex100 benzaldehyde and 0.2 ml of 20 %\ul0\nosupersub\cf10\f11\fs19 \ul0\nosup
ersub\cf1\f2\fs27 NaOH. Shake vigorously 25 times. Let \up0 \expndtw-4\charscale
x100 stand 2 minutes and add 5 ml benzene - n - butanol mixture. Follow \line \u
p0 \expndtw-4\charscalex100 procedure mentioned above beginning " transfer upper
layer with fine tip \up0 \expndtw-3\charscalex100 tube equipped with rubber bul

b to previously prepared CAS column \line \up0 \expndtw-3\charscalex100 avoiding

transfer of any aqueous phase" \par\pard\qj \li2111\sb0\sl-460\slmult0 \par\par
d\qj\li2111\ri1756\sb40\sl-460\slmult0 \up0 \expndtw-4\charscalex100 Substract b
lank A from A of standard (A) and sample (A) and calculate \up0 \expndtw-5\chars
calex100 histamine as under \par\pard\ql \li2111\sb0\sl-310\slmult0 \par\pard\ql
\li2111\sb0\sl-310\slmult0 \par\pard\ql\li2111\sb144\sl-310\slmult0 \up0 \expndt
w-1\charscalex100 Histamine , mg = ? Ax 25 \par\pard\ql \li3813\sb0\sl-299\slmul
t0 \up0 \expndtw-5\charscalex100 ? A \par\pard\qj \li2111\sb0\sl-320\slmult0 \pa
r\pard\qj\li2111\sb0\sl-320\slmult0 \par\pard\qj\li2111\ri1550\sb304\sl-320\slmu
lt0 \up0 \expndtw-4\charscalex100 (Ref: - A.O.A.C 17\ul0\super\cf17\f18\fs26 th\
ul0\nosupersub\cf1\f2\fs27 edn ,2000 Official method 957.07 Histamine in sea \u
p0 \expndtw-4\charscalex100 food - chemical) \par\pard\qj \li2111\ri2176\sb300\s
l-320\slmult0 \up0 \expndtw-4\charscalex100 (A Fluorimetric method - A.O.A.C Off
icial method 977. 13 is also \up0 \expndtw-4\charscalex100 available as another
alternative) \par\pard\ql \li1420\sb0\sl-356\slmult0 \par\pard\ql\li1420\sb275\s
l-356\slmult0 \up0 \expndtw0\charscalex94 \ul0\nosupersub\cf7\f8\fs31 2.0\ul0\no
supersub\cf24\f25\fs31 \ul0\nosupersub\cf7\f8\fs31 Dried Fish \par\pard\ql \li1
420\sb302\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\
fs27 2.1\ul0\nosupersub\cf22\f23\fs27 \ul0\nosupersub\cf14\f15\fs27 Sampling \p
ar\pard\qj \li2111\ri1867\sb310\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul
0\nosupersub\cf1\f2\fs27 Cut large pieces into small size and mix. Grind the pie
ces to obtain a \line \up0 \expndtw-4\charscalex100 homogeneous mass. Transfer t
o an airtight container to prevent loss of \up0 \expndtw-5\charscalex100 moistur
e \par\pard\ql \li2551\sb0\sl-310\slmult0 \par\pard\ql\li2551\sb0\sl-310\slmult0
\par\pard\ql\li2551\sb310\sl-310\slmult0 \up0 \expndtw-4\charscalex100 (Ref:- I
.S 14950 : 2001 Fish Dry and dry salted) \par\pard\ql \li5867\sb0\sl-218\slmult0
\par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \
par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb34\sl-218\slmult0 \u
p0 \expndtw-3\charscalex100 \ul0\nosupersub\cf10\f11\fs19 33
{\shp {\*\shpinst\shpleft2083\shptop2128\shpright10550\shpbottom2596\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft2083\shptop3061\shpright10550\shpbottom3529\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft2083\shptop3997\shpright10550\shpbottom4463\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft2083\shptop5408\shpright10044\shpbottom5874\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft1392\shptop7093\shpright10550\shpbottom7408\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft4190\shptop7372\shpright5073\shpbottom7392\shpfhdr0\shp
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft2083\shptop9287\shpright10550\shpbottom9908\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft2083\shptop9908\shpright10550\shpbottom10228\shpfhdr0\s
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{\shp {\*\shpinst\shpleft1392\shptop10852\shpright10550\shpbottom11209\shpfhdr0\
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{\shp {\*\shpinst\shpleft2083\shptop12131\shpright10550\shpbottom12443\shpfhdr0\
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft2083\shptop12443\shpright10550\shpbottom12757\shpfhdr0\
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{\shp {\*\shpinst\shpleft2083\shptop12757\shpright10550\shpbottom13072\shpfhdr0\
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{\shp {\*\shpinst\shpleft1401\shptop14005\shpright10550\shpbottom14320\shpfhdr0\
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{\shp {\*\shpinst\shpleft1392\shptop14320\shpright10550\shpbottom14632\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g36}{\bkmkend Pg36}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
b0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0
\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\s
l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li1420\sb0\sl-310\slmult0 \par\pard\ql\li1420\sb0\sl-310\slmult
0 \par\pard\ql\li1420\sb212\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\no

supersub\cf14\f15\fs27 2.2 Determination of moisture \par\pard\qj \li1411\ri1271

\sb170\sl-480\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27
Weigh accurately about 5 gm of the prepared sample in a moisture dish with slip
\line \up0 \expndtw-4\charscalex100 on cover. Dry in an air oven at 100 \ul0\nos
upersub\cf16\f17\fs27 \u8212? \up0 \expndtw-5\charscalex100 \ul0\nosupersub\cf1\
f2\fs27 1 C for 5 hours. Place lid on the dish and \par\pard\qj \li1411\ri1252\s
b0\sl-470\slmult0 \up0 \expndtw-4\charscalex100 cool in a dessicator. Quickly we
igh the dish. Return the dish with the cover to the \up0 \expndtw-3\charscalex10
0 oven and dry for another 1/2 hour. Cool in the dessicator and weigh again. Rep
eat \up0 \expndtw-4\charscalex100 until successive weights do not differ by more
than 1 mg. \par\pard\ql \li1420\sb0\sl-310\slmult0 \par\pard\ql\li1420\sb122\sl
-310\slmult0 \up0 \expndtw-5\charscalex100 Calculate moisture as under \par\pard
\ql \li1411\sb310\sl-310\slmult0 \up0 \expndtw0\charscalex103 Moisture = M\ul0\s
ub\cf17\f18\fs26 1\ul0\nosupersub\cf1\f2\fs27 x100 \par\pard\ql \li3232\sb58\sl253\slmult0 \up0 \expndtw-3\charscalex100 M\ul0\sub\cf17\f18\fs26 2 \par\pard\ql
\li1420\sb0\sl-310\slmult0 \par\pard\ql\li1420\sb10\sl-310\slmult0 \up0 \expndt
w-4\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Where \par\pard\ql \li2827\sb10\sl
-310\slmult0 \up0 \expndtw-5\charscalex100 M \ul0\sub\cf17\f18\fs26 i\ul0\nosupe
rsub\cf1\f2\fs27 = Loss in gm in the mass of sample \par\pard\ql \li2812\sb0\sl
-299\slmult0 \up0 \expndtw-5\charscalex100 M \ul0\sub\cf17\f18\fs26 2\ul0\nosupe
rsub\cf1\f2\fs27 \ul0\super\cf17\f18\fs26 =\ul0\nosupersub\cf1\f2\fs27 Mass in
gm of the sample taken for test \par\pard\ql \li2111\sb0\sl-310\slmult0 \par\pa
rd\ql\li2111\sb0\sl-310\slmult0 \par\pard\ql\li2111\sb0\sl-310\slmult0 \par\pard
\ql\li2111\sb0\sl-310\slmult0 \par\pard\ql\li2111\sb12\sl-310\slmult0 \up0 \expn
dtw-4\charscalex100 (Ref:- I.S 14950 : 2001 Fish Dry and dry salted) \par\pard\q
l \li1420\sb0\sl-310\slmult0 \par\pard\ql\li1420\sb0\sl-310\slmult0 \par\pard\ql
\li1420\sb0\sl-310\slmult0 \par\pard\ql\li1420\sb20\sl-310\slmult0 \up0 \expndtw
-5\charscalex100 \ul0\nosupersub\cf14\f15\fs27 2.3 Determination of Sodium Chlor
ide \par\pard\ql \li1420\sb310\sl-310\slmult0 \up0 \expndtw-4\charscalex100 2.3.
1 Reagents \par\pard\ql \li2136\sb310\sl-310\slmult0 \up0 \expndtw-4\charscalex1
00 \ul0\nosupersub\cf1\f2\fs27 (1)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub
\cf1\f2\fs27 Standard Silver Nitrate - 0.1 N \par\pard\ql \li2136\sb70\sl-310\sl
mult0 \up0 \expndtw-4\charscalex100 (2)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosup
ersub\cf1\f2\fs27 Dilute Nitric acid - 1 + 4 \par\pard\qj \li2136\ri2660\sb0\sl320\slmult0\tx2517 \up0 \expndtw-6\charscalex100 (3)\ul0\nosupersub\cf15\f16\fs2
7 \ul0\nosupersub\cf1\f2\fs27 Ferric alum indicator - Prepare a saturated solut
ion of Ferric \line\tab \up0 \expndtw-6\charscalex100 ammonium sulphate \par\par
d\ql \li2136\sb0\sl-274\slmult0 \up0 \expndtw-4\charscalex100 (4)\ul0\nosupersub
\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Standard Potassium thiocyanate solut
ion - 0.1 N \par\pard\ql \li1411\sb0\sl-310\slmult0 \par\pard\ql\li1411\sb27\sl310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 2.3.2 Pr
ocedure \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\s
lmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slm
ult0 \par\pard\ql\li5867\sb134\sl-218\slmult0 \up0 \expndtw-3\charscalex100 \ul0
\nosupersub\cf10\f11\fs19 34
{\shp {\*\shpinst\shpleft1392\shptop2128\shpright10550\shpbottom2440\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9158,312);(9158,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1392\shptop2440\shpright10550\shpbottom2754\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

\sn geoRight}{\sv 9158}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9158,314);(9158,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1382\shptop2941\shpright10550\shpbottom3407\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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}}
{\shp {\*\shpinst\shpleft1382\shptop3407\shpright10550\shpbottom3875\shpfhdr0\sh
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}}
{\shp {\*\shpinst\shpleft1382\shptop3875\shpright10550\shpbottom4343\shpfhdr0\sh
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{\sp{\sn pVerticies}{\sv 8;4;(0,465);(9168,465);(9168,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft1382\shptop4808\shpright10550\shpbottom5276\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(9168,468);(9168,0);(0,0)}}
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{\shp {\*\shpinst\shpleft1392\shptop5716\shpright10550\shpbottom6028\shpfhdr0\sh
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{\shp {\*\shpinst\shpleft3153\shptop6620\shpright3741\shpbottom6640\shpfhdr0\shp
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft2107\shptop12340\shpright10550\shpbottom12649\shpfhdr0\
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{\shp {\*\shpinst\shpleft1382\shptop13888\shpright10550\shpbottom14200\shpfhdr0\
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}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g37}{\bkmkend Pg37}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
b0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0
\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\s
l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li2107\sb0\sl-465\slmult0 \par\pard\ql\li2107\ri1193\sb59\sl-46
5\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Take 1-2 gm
of the dried material (obtained after determination of moisture) \up0 \expndtw-1
\charscalex100 in a 250 ml beaker and add 50 ml of distilled water free from chl
oride and \up0 \expndtw1\charscalex100 heat on a water bath till all the Sod.Chl
oride goes into solution. Filter in a \up0 \expndtw-2\charscalex100 250 ml conic
al flask and wash with distilled water till the washings are free \up0 \expndtw5\charscalex100 from chloride. Add 20 ml of dilute nitric acid and a known volum
e of \line \up0 \expndtw-1\charscalex100 standard silver nitrate sufficient to p
recipitate all the chloride. Add 1 ml of \up0 \expndtw-2\charscalex100 ferric al
um indicator and titrate with standard Potassium thiocyanate \line \up0 \expndtw
-2\charscalex100 solution until a permanent light brown colour appears \par\pard
\ql \li2116\sb0\sl-310\slmult0 \par\pard\ql\li2116\sb134\sl-310\slmult0 \up0 \ex
pndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 2.3.3 Calculation \par\pard\
ql \li2121\sb0\sl-310\slmult0 \par\pard\ql\li2121\sb0\sl-310\slmult0 \par\pard\q
l\li2121\sb0\sl-310\slmult0 \par\pard\ql\li2121\sb0\sl-310\slmult0 \par\pard\ql\
li2121\sb30\sl-310\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\
fs27 Sodium Chloride \par\pard\ql \li2822\sb170\sl-310\slmult0 \up0 \expndtw0\ch
arscalex103 (on dry basis) m / m = 5.85 x (V\ul0\sub\cf17\f18\fs26 1\ul0\nosuper
sub\cf1\f2\fs27 N\ul0\sub\cf17\f18\fs26 1\ul0\nosupersub\cf1\f2\fs27 -V\ul0\sub\
cf17\f18\fs26 2\ul0\nosupersub\cf1\f2\fs27 N\ul0\sub\cf17\f18\fs26 2\ul0\nosuper
sub\cf1\f2\fs27 ) \par\pard\ql \li7017\sb170\sl-310\slmult0 \up0 \expndtw-4\char
scalex100 M \par\pard\ql \li2121\sb0\sl-310\slmult0 \par\pard\ql\li2121\sb0\sl-3
10\slmult0 \par\pard\ql\li2121\sb10\sl-310\slmult0 \up0 \expndtw-5\charscalex100
Where \par\pard\ql \li3523\sb190\sl-310\slmult0 \up0 \expndtw-3\charscalex100 V
\ul0\nosupersub\cf17\f18\fs18 I\ul0\nosupersub\cf1\f2\fs27 = Vol of standard so
lution of silver nitrate \par\pard\qj \li3523\ri1470\sb43\sl-440\slmult0 \up0 \e
xpndtw-4\charscalex100 N\ul0\nosupersub\cf17\f18\fs18 l\ul0\super\cf1\f2\fs40 =
Normality of standard silver nitrate solution V\ul0\nosupersub\cf17\f18\fs18 2\
ul0\super\cf1\f2\fs40 = Vol of \up0 \expndtw-4\charscalex100 standard Pot. Thio
cyanate solution \par\pard\qj \li3513\ri2865\sb4\sl-460\slmult0\fi9 \up0 \expndt
w-5\charscalex100 N\ul0\sub\cf17\f18\fs26 2\ul0\nosupersub\cf1\f2\fs27 = Normal
ity of standard Pot. Thiocyanate sol \up0 \expndtw-5\charscalex100 M = Mass of d
ried material taken for test \par\pard\ql \li2812\sb0\sl-310\slmult0 \par\pard\q
l\li2812\sb0\sl-310\slmult0 \par\pard\ql\li2812\sb0\sl-310\slmult0 \par\pard\ql\
li2812\sb294\sl-310\slmult0 \up0 \expndtw-2\charscalex100 (Ref:- I.S 14950 : 200
1 Fish Dry and dry salted) \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\
li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li
5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li58
67\sb156\sl-218\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf10\f11\f
s19 35
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{\shp {\*\shpinst\shpleft2784\shptop11984\shpright10550\shpbottom12452\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz433\shplid27
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 7766}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(7766,468);(7766,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2097\shptop12896\shpright10550\shpbottom13208\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz452\shplid28
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8453}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(8453,312);(8453,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2097\shptop13208\shpright10550\shpbottom13967\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz465\shplid29
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8453}}{\sp{\sn geoBottom}{\sv 759}}
{\sp{\sn pVerticies}{\sv 8;4;(0,759);(8453,759);(8453,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft1396\shptop13967\shpright10550\shpbottom14588\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz486\shplid30
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9154}}{\sp{\sn geoBottom}{\sv 621}}

{\sp{\sn pVerticies}{\sv 8;4;(0,621);(9154,621);(9154,0);(0,0)}}

{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g38}{\bkmkend Pg38}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
b0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0
\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\s
l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li1425\sb0\sl-310\slmult0 \par\pard\ql\li1425\sb0\sl-310\slmult
0 \par\pard\ql\li1425\sb152\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\no
supersub\cf19\f20\fs27 2.4 Determination of Ash insoluble in dil HCl \par\pard\q
l \li1416\sb310\sl-310\slmult0 \up0 \expndtw-3\charscalex100 2.4.1\ul0\nosupersu
b\cf14\f15\fs27 \ul0\nosupersub\cf19\f20\fs27 Reagent \par\pard\ql \li1411\sb0\
sl-287\slmult0 \par\pard\ql\li1411\sb42\sl-287\slmult0 \up0 \expndtw0\charscalex
103 \ul0\nosupersub\cf25\f26\fs25 Dilute Hydrochloric acid - 1 + 1 \par\pard\ql
\li1416\sb0\sl-310\slmult0 \par\pard\ql\li1416\sb4\sl-310\slmult0 \up0 \expndtw4\charscalex100 \ul0\nosupersub\cf19\f20\fs27 2.4.2\ul0\nosupersub\cf14\f15\fs27
\ul0\nosupersub\cf19\f20\fs27 Procedure \par\pard\ql \li2111\ri1143\sb340\sl-4
68\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf18\f19\fs27 Weigh acc
urately 2 gm of the dried material (obtained after determination of \line \up0 \
expndtw-3\charscalex100 moisture) in a silica or platinum dish. Ignite on a burn
er till all organic \line \up0 \expndtw-3\charscalex100 matter is charred. Trans
fer to a muffle furnace maintained at 550 \ul0\super\cf20\f21\fs26 0\ul0\nosuper
sub\cf18\f19\fs27 C and \line \up0 \expndtw-3\charscalex100 keep for few hours
till grey ash is obtained. Cool in a desiccator. Weigh to \line \up0 \expndtw-2\
charscalex100 determine total ash, if desired. Add 25 - 30 ml of dilute HCl to t
he dish and \line \up0 \expndtw-2\charscalex100 boil it for 10 minutes. Cool and
filter it through Whatman filter paper No 42 \line \up0 \expndtw-3\charscalex10
0 or its equivalent. Wash the residue with water until the washings are free \li
ne \up0 \expndtw-3\charscalex100 from chloride as tested with silver nitrate. Re
turn the filter paper and residue \line \up0 \expndtw-3\charscalex100 to the dis
h. Dry in an air oven for 2 hours and ignite in the muffle furnace \line \up0 \e
xpndtw-4\charscalex100 for 1 hour. Cool and weigh. Return the dish to the furnac
e again for 30 \line \up0 \expndtw-4\charscalex100 minutes, cool and weigh again
. Repeat the process till the difference \line \up0 \expndtw-5\charscalex100 bet
ween two successive weighings is not more than 1 mg. Note the
lowest \line
\up0 \expndtw-6\charscalex100 weight. \par\pard\ql \li1416\sb0\sl-310\slmult0 \p
ar\pard\ql\li1416\sb133\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupe
rsub\cf19\f20\fs27 2.4.3\ul0\nosupersub\cf14\f15\fs27 \ul0\nosupersub\cf19\f20\
fs27 Calculation \par\pard\ql \li2126\sb310\sl-310\slmult0 \up0 \expndtw-5\chars
calex100 \ul0\nosupersub\cf18\f19\fs27 Ash insoluble in dil HCl \par\pard\ql \li
3295\sb10\sl-310\slmult0 \up0 \expndtw-3\charscalex100 (on dry basis) m / m = 10
0 x (M\ul0\sub\cf20\f21\fs26 2\ul0\nosupersub\cf18\f19\fs27 - M) \par\pard\ql \
li6549\sb30\sl-310\slmult0 \up0 \expndtw0\charscalex100 M\ul0\nosupersub\cf20\f2
1\fs17 l\ul0\nosupersub\cf18\f19\fs27 - M Where \par\pard\qj \li2832\ri3332\s
b0\sl-320\slmult0\fi4 \up0 \expndtw-4\charscalex100 M \ul0\sub\cf20\f21\fs26 2\u
l0\nosupersub\cf18\f19\fs27 = Lowest mass of dish with acid insoluble ash \up0
\expndtw-4\charscalex100 M = Mass of empty dish \par\pard\ql \li2832\sb0\sl-274\
slmult0 \up0 \expndtw-3\charscalex100 M \ul0\sub\cf20\f21\fs26 l\ul0\nosupersub\
cf18\f19\fs27 = Mass of dish with the dried material taken for test. \par\pard\
ql \li2846\sb0\sl-310\slmult0 \par\pard\ql\li2846\sb7\sl-310\slmult0 \up0 \expnd
tw-2\charscalex100 \ul0\nosupersub\cf1\f2\fs27 (Ref:- I.S 14950 : 2001 Fish Dry
and dry salted) \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\
sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb12\s
l-218\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf10\f11\fs19 36

{\shp {\*\shpinst\shpleft1396\shptop2128\shpright10550\shpbottom2749\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9154}}{\sp{\sn geoBottom}{\sv 621}}
{\sp{\sn pVerticies}{\sv 8;4;(0,621);(9154,621);(9154,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1387\shptop2749\shpright10550\shpbottom3371\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz60\shplid1
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9163}}{\sp{\sn geoBottom}{\sv 622}}
{\sp{\sn pVerticies}{\sv 8;4;(0,622);(9163,622);(9163,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1382\shptop3376\shpright10550\shpbottom3997\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz90\shplid2
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9168}}{\sp{\sn geoBottom}{\sv 621}}
{\sp{\sn pVerticies}{\sv 8;4;(0,621);(9168,621);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1387\shptop3997\shpright10550\shpbottom4616\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz113\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9163}}{\sp{\sn geoBottom}{\sv 619}}
{\sp{\sn pVerticies}{\sv 8;4;(0,619);(9163,619);(9163,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft2083\shptop4904\shpright10550\shpbottom5375\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz143\shplid4
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 471}}
{\sp{\sn pVerticies}{\sv 8;4;(0,471);(8467,471);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft2083\shptop5375\shpright10550\shpbottom5843\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz158\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft2083\shptop5843\shpright10550\shpbottom6308\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz168\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(8467,465);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop6308\shpright10550\shpbottom6776\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz196\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft2083\shptop6776\shpright10550\shpbottom7244\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz221\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2083\shptop7244\shpright10550\shpbottom7710\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz247\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(8467,466);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}

{\shp {\*\shpinst\shpleft2083\shptop7710\shpright10550\shpbottom8178\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz261\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop8178\shpright10550\shpbottom8646\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz271\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop8646\shpright10550\shpbottom9112\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,466);(8467,466);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop9112\shpright10550\shpbottom9580\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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{\shp {\*\shpinst\shpleft2083\shptop9580\shpright10550\shpbottom10048\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz305\shplid14
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}

{\shp {\*\shpinst\shpleft2083\shptop10048\shpright10550\shpbottom10513\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz319\shplid15
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{\sp{\sn pVerticies}{\sv 8;4;(0,465);(8467,465);(8467,0);(0,0)}}
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{\shp {\*\shpinst\shpleft6501\shptop12637\shpright6835\shpbottom12657\shpfhdr0\s
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{\shp {\*\shpinst\shpleft6924\shptop12637\shpright7490\shpbottom12657\shpfhdr0\s
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{\shp {\*\shpinst\shpleft1392\shptop12668\shpright9035\shpbottom12980\shpfhdr0\s
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft2808\shptop12980\shpright10550\shpbottom13295\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft2803\shptop13295\shpright10550\shpbottom13609\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz529\shplid25
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft2803\shptop13609\shpright10550\shpbottom13921\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft2817\shptop13921\shpright10550\shpbottom14236\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft2817\shptop14236\shpright10550\shpbottom14548\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz597\shplid28
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g39}{\bkmkend Pg39}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
b0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0
\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\s
l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li1411\sb0\sl-356\slmult0 \par\pard\ql\li1411\sb158\sl-356\slmu
lt0 \up0 \expndtw0\charscalex97 \ul0\nosupersub\cf7\f8\fs31 3.0 Canned Fish \par
\pard\ql \li1411\sb0\sl-310\slmult0 \par\pard\ql\li1411\sb12\sl-310\slmult0 \up0
\expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 3.1Preparation of sample
\par\pard\qj \li1416\ri2246\sb154\sl-500\slmult0 \up0 \expndtw-3\charscalex100
\ul0\nosupersub\cf1\f2\fs27 Place entire contents of can (meat and liquid) in a
blender and blend until \up0 \expndtw-4\charscalex100 homogeneous or grind three
times through meat chopper. \par\pard\qj \li1411\sb0\sl-480\slmult0 \par\pard\q

j\li1411\ri1247\sb77\sl-480\slmult0 \up0 \expndtw-4\charscalex100 (Ref: - A,O,A,

C 17\ul0\super\cf17\f18\fs26 th\ul0\nosupersub\cf1\f2\fs27 edn,2000, Official m
ethod 937.07 Fish and Marine Products -\line \up0 \expndtw-5\charscalex100 Treat
ment and Preparation of Sample) \par\pard\ql \li1411\sb0\sl-310\slmult0 \par\par
d\ql\li1411\sb131\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\c
f14\f15\fs27 3.2Determination of Acidity of Brine \par\pard\ql \li1411\sb310\sl310\slmult0 \up0 \expndtw-6\charscalex100 3.2.1Reagents \par\pard\ql \li2131\sb3
10\sl-310\slmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf1\f2\fs27 (1)\
ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\f2\fs27 Standard Sodium Hydrox
ide solution - 0.1 N \par\pard\qj \li2131\ri1894\sb47\sl-460\slmult0\tx2512 \up0
\expndtw-4\charscalex100 (2)\ul0\nosupersub\cf15\f16\fs27 \ul0\nosupersub\cf1\
f2\fs27 Phenolpthalein indicator solution - Dissolve 1 gm phenolphthalein \line\
tab \up0 \expndtw-4\charscalex100 in 100 ml of 95% alcohol \par\pard\ql \li1411\
sb0\sl-310\slmult0 \par\pard\ql\li1411\sb154\sl-310\slmult0 \up0 \expndtw-5\char
scalex100 \ul0\nosupersub\cf14\f15\fs27 3.2.2Procedure \par\pard\ql \li2111\sb31
0\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Take
25- 40 ml of the brine solution,( previously filtered to remove \par\pard\ql \li
2111\ri1466\sb18\sl-470\slmult0 \up0 \expndtw-3\charscalex100 suspended matter i
f any) in a 200 ml flask., add 25- 50 ml water if desired \line \up0 \expndtw-4\
charscalex100 and titrate against standard Sodium hydroxide solution using \line
\up0 \expndtw-3\charscalex100 phenolphthalein as indicator till a faint pink co
lour persists for 15 seconds \par\pard\ql \li1411\sb0\sl-310\slmult0 \par\pard\q
l\li1411\sb133\sl-310\slmult0 \up0 \expndtw-5\charscalex100 \ul0\nosupersub\cf14
\f15\fs27 3.2.3Calculation \par\pard\ql \li2841\sb310\sl-310\slmult0 \up0 \expnd
tw-4\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Acidity as citric acid (m/m) = 0.
0070 x ml 0. IN NaOH x 100 \par\pard\ql \li6386\sb10\sl-310\slmult0 \up0 \expndt
w-3\charscalex100 Volume of brine taken \par\pard\ql \li2121\sb0\sl-310\slmult0
\par\pard\ql\li2121\sb200\sl-310\slmult0 \up0 \expndtw-1\charscalex100 ( Ref:- I
.S.I Handbook of Food Analysis (Part XII) - 1984, page 50 ) \ul0\nosupersub\cf14
\f15\fs27 3.3 \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl
-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-2
18\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb56\sl-21
8\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf10\f11\fs19 37
{\shp {\*\shpinst\shpleft1382\shptop2128\shpright10550\shpbottom2485\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,357);(9168,357);(9168,0);(0,0)}}
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}}
{\shp {\*\shpinst\shpleft1382\shptop2485\shpright10550\shpbottom3109\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft1387\shptop3272\shpright10550\shpbottom3771\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

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{\shp {\*\shpinst\shpleft1387\shptop3771\shpright10550\shpbottom4273\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz91\shplid3
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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}}
{\shp {\*\shpinst\shpleft1382\shptop4842\shpright10550\shpbottom5310\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft1382\shptop5310\shpright10550\shpbottom5776\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz155\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\shp {\*\shpinst\shpleft1382\shptop5776\shpright10550\shpbottom6534\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

\sn geoRight}{\sv 9168}}{\sp{\sn geoBottom}{\sv 622}}
{\sp{\sn pVerticies}{\sv 8;4;(0,622);(9168,622);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2102\shptop7340\shpright10550\shpbottom7808\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz213\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8448}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8448,468);(8448,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2102\shptop7808\shpright10550\shpbottom8284\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz252\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,476);(8448,476);(8448,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2102\shptop8284\shpright10550\shpbottom8749\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz294\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,465);(8448,465);(8448,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1382\shptop9198\shpright10550\shpbottom9512\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz308\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9168}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(9168,314);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2083\shptop9690\shpright10550\shpbottom10156\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz327\shplid12

{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 466}}
{\sp{\sn pVerticies}{\sv 8;4;(0,466);(8467,466);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2083\shptop10156\shpright10550\shpbottom10624\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz346\shplid13
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8467}}{\sp{\sn geoBottom}{\sv 468}}
{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2083\shptop10624\shpright10550\shpbottom11092\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz364\shplid14
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8467,468);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop11092\shpright10550\shpbottom11557\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz378\shplid15
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,465);(8467,465);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1382\shptop11996\shpright10550\shpbottom12311\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz393\shplid16
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,315);(9168,315);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2812\shptop12618\shpright10550\shpbottom12932\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz412\shplid17

{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{

\sn geoRight}{\sv 7738}}{\sp{\sn geoBottom}{\sv 314}}
{\sp{\sn pVerticies}{\sv 8;4;(0,314);(7738,314);(7738,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft6141\shptop12896\shpright9544\shpbottom12916\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz430\shplid18
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,20);(3403,20);(3403,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 0}}{\s
p{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv 1}}{\s
p{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1382\shptop12932\shpright10550\shpbottom13244\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz440\shplid19
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9168}}{\sp{\sn geoBottom}{\sv 312}}
{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9168,312);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2092\shptop13244\shpright10550\shpbottom14188\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz462\shplid20
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8458}}{\sp{\sn geoBottom}{\sv 944}}
{\sp{\sn pVerticies}{\sv 8;4;(0,944);(8458,944);(8458,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g40}{\bkmkend Pg40}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
b0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0
\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\s
l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li2121\sb0\sl-310\slmult0 \par\pard\ql\li2121\sb0\sl-310\slmult
0 \par\pard\ql\li2121\sb0\sl-310\slmult0 \par\pard\ql\li2121\sb102\sl-310\slmult
0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf14\f15\fs27 3.30 Modified Star
ches in the packing medium (Appendix C) \par\pard\ql \li2111\sb0\sl-310\slmult0
\par\pard\ql\li2111\sb0\sl-310\slmult0 \par\pard\ql\li2111\sb10\sl-310\slmult0 \
up0 \expndtw-4\charscalex100 3.3.1Detection \par\pard\ql \li2116\ri1226\sb338\sl
-470\slmult0 \up0 \expndtw-2\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Add a few
drop of iodine solution to a small portion of the packing medium \up0 \expndtw2\charscalex100 in a test tube. Boil for a minute. The presence of dark blue to
red colour \up0 \expndtw-4\charscalex100 indicates presence of starch \par\pard\

qj \li2111\sb0\sl-460\slmult0 \par\pard\qj\li2111\ri1187\sb19\sl-460\slmult0 \up

0 \expndtw-2\charscalex100 (Ref:- Specifications for identity and purity of cert
ain food additives - F.A.O \up0 \expndtw-4\charscalex100 Food and Nutrition Pape
r 49 , page 29 ) \par\pard\ql \li2111\sb0\sl-310\slmult0 \par\pard\ql\li2111\sb1
34\sl-310\slmult0 \up0 \expndtw-5\charscalex100 \ul0\nosupersub\cf14\f15\fs27 3.
3.2 Quantitative Determination \par\pard\ql \li2121\sb0\sl-310\slmult0 \par\pard
\ql\li2121\sb0\sl-310\slmult0 \par\pard\ql\li2121\sb170\sl-310\slmult0 \up0 \exp
ndtw-5\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Take 50 ml of the packing mediu
m. Precipitate starch by adding 95 % \par\pard\ql \li2121\ri1237\sb20\sl-468\sl
mult0 \up0 \expndtw-3\charscalex100 alcohol. Centrifuge, throw away the supernat
ant liquid. Wash residue with \up0 \expndtw-3\charscalex100 water in a boiling f
lask. Add 10 ml of dilute HCl (1+1) and about 50 - 70 \up0 \expndtw-2\charscalex
100 ml of water, mix and reflux for 2 hours. Cool. Make the solution alkaline by
\up0 \expndtw-5\charscalex100 adding cone Sodium hydroxide and sodium carbonate
at the end. Make up \up0 \expndtw-6\charscalex100 to 100 ml Determine reducing
sugars using Lane and Eynon method and \up0 \expndtw-6\charscalex100 convert tot
al sugars to starch by multiplying with 0.9 \par\pard\qj \li2131\sb0\sl-460\slmu
lt0 \par\pard\qj\li2131\ri1746\sb19\sl-460\slmult0 \up0 \expndtw0\charscalex101
(Ref:- A.O.A.C 17\ul0\super\cf17\f18\fs26 th\ul0\nosupersub\cf1\f2\fs27 edn 200
0, Modified Official Method 925.5. for \up0 \expndtw0\charscalex102 Starch in Co
nfectionery) \par\pard\ql \li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-21
8\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\
slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\sl
mult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb138\sl-218\sl
mult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf10\f11\fs19 38
{\shp {\*\shpinst\shpleft2092\shptop2128\shpright10550\shpbottom3071\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8458}}{\sp{\sn geoBottom}{\sv 943}}
{\sp{\sn pVerticies}{\sv 8;4;(0,943);(8458,943);(8458,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft1382\shptop3071\shpright10550\shpbottom4014\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz61\shplid1
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9168}}{\sp{\sn geoBottom}{\sv 943}}
{\sp{\sn pVerticies}{\sv 8;4;(0,943);(9168,943);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2088\shptop4225\shpright10550\shpbottom4698\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz77\shplid2
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8462}}{\sp{\sn geoBottom}{\sv 473}}
{\sp{\sn pVerticies}{\sv 8;4;(0,473);(8462,473);(8462,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv

1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}

}}
{\shp {\*\shpinst\shpleft2088\shptop4698\shpright10550\shpbottom5171\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,473);(8462,473);(8462,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2088\shptop5171\shpright10550\shpbottom5644\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz110\shplid4
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,473);(8462,473);(8462,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop6107\shpright10550\shpbottom6577\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz125\shplid5
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,470);(8467,470);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2083\shptop6577\shpright10550\shpbottom7050\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz151\shplid6
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,473);(8467,473);(8467,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1382\shptop7484\shpright10550\shpbottom7796\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz166\shplid7
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,312);(9168,312);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2092\shptop8452\shpright10550\shpbottom8917\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz182\shplid8
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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}}
{\shp {\*\shpinst\shpleft2092\shptop8917\shpright10550\shpbottom9385\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz209\shplid9
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8458,468);(8458,0);(0,0)}}
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}}
{\shp {\*\shpinst\shpleft2092\shptop9385\shpright10550\shpbottom9853\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz219\shplid10
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2092\shptop9853\shpright10550\shpbottom10319\shpfhdr0\s
hpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz253\shplid11
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,466);(8458,466);(8458,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
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{\shp {\*\shpinst\shpleft2092\shptop10319\shpright10550\shpbottom10787\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv

1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}

}}
{\shp {\*\shpinst\shpleft2092\shptop10787\shpright10550\shpbottom11255\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8458,468);(8458,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
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}}
{\shp {\*\shpinst\shpleft2092\shptop11255\shpright10550\shpbottom11720\shpfhdr0\
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8458}}{\sp{\sn geoBottom}{\sv 465}}
{\sp{\sn pVerticies}{\sv 8;4;(0,465);(8458,465);(8458,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2102\shptop12316\shpright10550\shpbottom12784\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz318\shplid15
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,468);(8448,468);(8448,0);(0,0)}}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2102\shptop12784\shpright10550\shpbottom13254\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz346\shplid16
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8448}}{\sp{\sn geoBottom}{\sv 470}}
{\sp{\sn pVerticies}{\sv 8;4;(0,470);(8448,470);(8448,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv
1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}
}}
{\shp {\*\shpinst\shpleft2102\shptop13254\shpright10550\shpbottom14183\shpfhdr0\
shpbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz360\shplid17
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8448}}{\sp{\sn geoBottom}{\sv 929}}
{\sp{\sn pVerticies}{\sv 8;4;(0,929);(8448,929);(8448,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
}
{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
15}}{\sp{\sn fLine}{\sv 0}}{\sp{\sn lineType}{\sv 0}}{\sp{\sn fArrowheadsOK}{\sv

1}}{\sp{\sn fBehindDocument}{\sv 1}}{\sp{\sn lineColor}{\sv 0}}

}}\par\pard\sect\sectd\fs24\paperw11900\paperh16840\pard\sb0\sl-240{\bkmkstart P
g41}{\bkmkend Pg41}\par\pard\ql \li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\s
b0\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0
\sl-184\slmult0 \par\pard\ql\li5949\sb0\sl-184\slmult0 \par\pard\ql\li5949\sb0\s
l-184\slmult0 \par\pard\ql\li5949\sb84\sl-184\slmult0 \up0 \expndtw-2\charscalex
100 \ul0\nosupersub\cf9\f10\fs16 MEAT AND MEAT PRODUCTS AND FISH AND FISH PRODUC
TS \par\pard\ql \li1831\sb0\sl-310\slmult0 \par\pard\ql\li1831\sb0\sl-310\slmult
0 \par\pard\ql\li1831\sb0\sl-310\slmult0 \par\pard\ql\li1831\sb82\sl-310\slmult0
\up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\fs27 3.4 Determination o
f Ascorbic acid (Appendix C) \par\pard\ql \li2126\sb0\sl-310\slmult0 \par\pard\q
l\li2126\sb0\sl-310\slmult0 \par\pard\ql\li2126\sb30\sl-310\slmult0 \up0 \expndt
w-4\charscalex100 \ul0\nosupersub\cf1\f2\fs27 Follow method given in Meat and Me
at Products Clause 1.2 \par\pard\ql \li1831\sb0\sl-310\slmult0 \par\pard\ql\li18
31\sb300\sl-310\slmult0 \up0 \expndtw-4\charscalex100 \ul0\nosupersub\cf14\f15\f
s27 3.5 Determination \ul0\nosupersub\cf1\f2\fs27 of polyphosphate ( Appendix C
) \par\pard\ql \li2126\sb0\sl-310\slmult0 \par\pard\ql\li2126\sb0\sl-310\slmult0
\par\pard\ql\li2126\sb10\sl-310\slmult0 \up0 \expndtw-4\charscalex100 Follow me
thod given in Meat and Meat Products Clause 1.4 \par\pard\ql \li1804\sb0\sl-310\
slmult0 \par\pard\ql\li1804\sb0\sl-310\slmult0 \par\pard\ql\li1804\sb30\sl-310\s
lmult0 \up0 \expndtw-3\charscalex100 \ul0\nosupersub\cf14\f15\fs27 3.6 Determina
tion of Sulphur Dioxide ( Appendix C \ul0\nosupersub\cf1\f2\fs27 ) \par\pard\ql
\li2121\sb0\sl-310\slmult0 \par\pard\ql\li2121\sb0\sl-310\slmult0 \par\pard\ql\l
i2121\sb10\sl-310\slmult0 \up0 \expndtw-3\charscalex100 Follow method given in P
rocessed Fruits and Vegetables clause 16.7 \par\pard\ql \li5867\sb0\sl-218\slmul
t0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0
\par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \
par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \pa
r\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\
pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pa
rd\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard
\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\q
l\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\
li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li
5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li58
67\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867
\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\s
b0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0
\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\s
l-218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl218\slmult0 \par\pard\ql\li5867\sb0\sl-218\slmult0 \par\pard\ql\li5867\sb0\sl-21
8\slmult0 \par\pard\ql\li5867\sb154\sl-218\slmult0 \up0 \expndtw-2\charscalex100
\ul0\nosupersub\cf10\f11\fs19 39
{\shp {\*\shpinst\shpleft1382\shptop2128\shpright10550\shpbottom3066\shpfhdr0\sh
pbxpage\shpbypage\shpwr3\shpwrk0\shpfblwtxt1\shpz38\shplid0
{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 9168}}{\sp{\sn geoBottom}{\sv 938}}
{\sp{\sn pVerticies}{\sv 8;4;(0,938);(9168,938);(9168,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft2097\shptop3066\shpright10550\shpbottom4007\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
\sn geoRight}{\sv 8453}}{\sp{\sn geoBottom}{\sv 941}}

{\sp{\sn pVerticies}{\sv 8;4;(0,941);(8453,941);(8453,0);(0,0)}}

{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1382\shptop4007\shpright10550\shpbottom4945\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,938);(9168,938);(9168,0);(0,0)}}
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}}
{\shp {\*\shpinst\shpleft2097\shptop4945\shpright10550\shpbottom5884\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,939);(8453,939);(8453,0);(0,0)}}
{\sp{\sn pSegmentInfo}{\sv 2;10;16384;45824;1;45824;1;45824;1;45824;24577;32768}
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{\sp{\sn fFillOK}{\sv 1}}{\sp{\sn fFilled}{\sv 1}}{\sp{\sn fillColor}{\sv 167772
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}}
{\shp {\*\shpinst\shpleft1382\shptop5888\shpright10550\shpbottom6829\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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{\sp{\sn pVerticies}{\sv 8;4;(0,941);(9168,941);(9168,0);(0,0)}}
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}}
{\shp {\*\shpinst\shpleft2092\shptop6829\shpright10550\shpbottom7770\shpfhdr0\sh
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{\sp{\sn shapeType}{\sv 0}}{\sp{\sn fFlipH}{\sv 0}}{\sp{\sn fFlipV}{\sv 0}}{\sp{
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}}\par\pard\sect\sectd\fs24}