Biotechnology Questions, Paper - 01

Questions
1. The term cistorn, muton and recon were introduced by (A) Watson and Crick (B) S. Benzer (C) Meselson (D) Morgan 2. Extranuclear genetic material is found in (A) Plastid and nucleus (B) Mitochondria and plastids (C) Nucleus and cytoplasm (D) Mitochondria and nucleus 3. The molecular formulae of deoxyribose sugar and ribose sugar respectively are

Answers
Answer: (B)

Answer: (B)

Answer: (B)

(A) C5 H10 O4 and C5H10O6 (B) C5 H10 O4 and C5H10O5 (C) C5 H10 O5 and C5H10O4 (D) C5 H10 O5 and C6H10O4
4. The nitrogen bases which pair with two hydrogen bonds are (A) Adenine and thymine (B) Adenine and Cytosine (C) Cytosine and guanine (D) Cytosine and adenine 5. DNA differs from RNA in (A) Presence of deoxyribose sugar (B) Presence of thymine base (C) Property of replication (D) All the above 6. DNA molecules makes a complete turn after every (A) 20 (B) 34 (B) 3.4 (D) 10 base pairs 7. The distance between two successive nitrogenous base pairs is (A) 34 (B) 36 (C) 20 (D) 3.4 8. In nucleoside, nitrogen base is attached to pentose sugar at (A) Carbon 1 of pentose sugar (B) Carbon 2 of pentose sugar (C) Carbon 4 of pentose sugar (D) Carbon 5 of pentose sugar 9. If the strand of DNA has 35 nucleotide how many phosphodiester bonds would exist (A) 34 (B) 35 (C) 24 (D) 70 10. In eukaryotic DNA replication, lagging strand is formed by Answer: (A)

Answer: (D)

Answer: (D)

Answer: (D)

Answer: (A)

Answer: (A)

Answer: (B)

(A) RNA fragments (B) Okazaki fragments (C) DNA fragments (D) Nucleotide fragments 11. The enzyme DNA polymerase can work only in Answer: (B)

(A) 3 5 direction (B) 5 3 direction (C) Both the direction (D) 5 5 direction
12. Enzyme required for removing RNA primer during DNA replication is (A) DNA primase (B) DNA ligase (C) DNA polymerase I (D) DNA polymerase III 13. During DNA replication, the reunion or recoiling of separated DNA strand is prevented by (A) Helix destabilizing protein (B) Single strnad binding protein (C) Rep protein (D) Both (A) and (B) 14. The enzyme that cuts the bonds of DNA molecule at the origin of replication is (A) Endonuclease (B) DNA polymerase (C) DNA gyrase (D) DNA ligase 15. Which of the following enzyme is required to release the tension imposed by uncoiling of strands? (A) Endonuclease (B) DNA ligase (C) DNA gyrase (D) DNA helicase 16. The cellular composition of m-RNA is (A) 5-10% (B) 3-5% (C) 10-20 % (D) 70-80% 17. Formation of mRNA from DNA is called (A) Transformation (B) Transduction (C) Traslation (D) Transcription 18. The ratio of purines and pyrimidines in mRNA is not 1:1 because the nitrogenous bases are (A) Unpaired (B) Paired (C) Paired only in loops (D) Paired in stems 19. The codons which may present at 3 end of mRNA (A) UAA (B) UAG (C) UGA (D) Any one of these 20. Which of the following is not tool of genetic engineering? Answer: (C)

Answer: (D)

Answer: (A)

Answer: (C)

Answer: (B)

Answer: (D)

Answer: (A)

Answer: (D)

Answer: (D)

(A) Vectors (B) Enzymes (C) Foreign DNA (D) GMO 21. In recombinant DNA technology a plasmid vector is cleaved by (A) Modified DNA ligase (B) A heated alkaline solution (C) The same enzyme that cleave the donor DNA (D) The different enzyme other than that cleave the donor DNA 22. The most common plasmid vector used in genetic engineering is (A) PBR 328 (B) PBR 322 (C) PBR 325 (D) PBR 330 23. Nif gene for nitrogen fixation is cereal crops like wheat, jowar etc. is introduced by cloning (A) Rhizobium meliloti (B) Bacillus thuringiensis (C) Rhizopus (D) Rhizophora 24. Eco RI is an (A) Ligase (B) Polymerase (C) Restriction enzyme (D) Gyrase 25. The transgenic plant flavr savr tomato carries an artificial gene for (A) Delay ripening process (B) Longer shell life (C) Added flavours (D) All of these Answer: (C)

Answer: (B)

Answer: (A)

Answer: (C)

Answer: (D)

Biotechnology Questions, Paper - 02

Questions
1. Hirudin is obtained from the transgenic plant (A) Brassica napus (B) Hibiscus rosasinesis (C) Raphanus sativus (D) Vinca rosea 2. Bt Cotton is (A) Cloned plant (B) Transgenic plant (C) Hybrid plant (D) Mutated plant 3. Dolly sheep was genetically similar to (A) The mother from which nucleated fertilized egg was taken (B) The mother from which nuclear DNA of udder cell was taken

Answers

Answer: (A)

Answer: (B)

Answer: (B)

(C) The surrogate mother (D) Both surrogate mother and nuclear donor mother 4. Genome is (A) Genes on nuclear DNA (B) Nuclear DNA + mitochondrial DNA (C) Nuclear DNA + chloroplast DNA (D) Nuclear DNA + Mitochondrial DNA + Chloroplast DNA 5. A technique of using very small metal particles coated with desired gene in the gene transfer is called (A) Electroporation (B) Microinjection (C) Liposome (D) Biolistics 6. The complete set of chromosomal and extrachromosomal genes of an organisms is called (A) Genome (B) Gene pool (C) Gene bank (D) Gene library 7. The study of all the proteins coded by the genome is called (A) Proteome (B) Proteomics (C) Genome (D) Protein formation 8. Sequencing of genomic DNA is included under (A) Structural genomics (B) Functional genomics (C) Proteomics (D) Transgenesis 9. Gene expression, regulation and phenotype production are studied in second phase of genome analysis called (A) Structural genomics (B) Functional genomics (C) Proteomics (D) Transmeiosis 10. A flowering plant lily have ______ more DNA than humans (A) 10 times (B) 15 times (C) 18 times (D) 13 times 11. In forensic science which of the following is used? (A) Bacterial cloning (B) DNA foot printing (C) DNA fingerprinting (D) DNA cloning 12. DNA fingerprinting is based on (A) Occurance of VNTRs (B) Knowledge of human karyotype (C) Cloned DNA (D) Recombinant DNA 13. VNTRs represnets(A) New terminal regions in DNA

Answer: (D)

Answer: (D)

Answer: (A)

Answer: (B)

Answer: (A)

Answer: (B)

Answer: (C)

Answer: (C)

Answer: (A)

Answer: (D)

(B) Functional genes in the DNA (C) Split genes in the sample DNA (D) Specific non-coding sequences with unique tandem repeats 14. Which ones produce androgenic haploids in anther cultures? (A) Anther wall (B) Tapetal layer of anther wall (C) Connective tissue (D) Young pollengrains 15. Variations observed during tissue culture of some plants are known as (A) Clonal variations (B) Somatic variations (C) Somaclonal variations (D) Tissue culture variations 16. Virus free plants can be obtained through (A) Anitibiotic treatment (B) Bordeaux micture (C) Root tip culture (D) Shoot tip culture 17. To raising of plants from a small tissue in culture is known as (A) Macroproduction (B) Micropropagation (C) Tissue culture (D) Mass production 18. Callus is (A) Tissue that forms embryo (B) an insoluble carbohydrate (C) Unorganised actively dividing mass of cells maintained in culture (D) Tissue that growth to form embryoid 19. Biopatents are ______. (A) Right to use invention (B) Right to use biological entities (C) Right to use products (D) Right to use process 20. African plant Pentadiplandra is used as______. (A) Low calories sweetner (B) 2000 times sweeter agent (C) Sweetner for diabetic patients (D) All of these 21. Which organism was used as bioweapon derived from______. (A) Clostridium (B) Yerstsinia pestis (C) Fusarium species (D) Green algae 22. A set standards used to regulate own or community activity in relation to biological world is (A) Biopotency (B) Biopiracy (C) Biowar (D) Bioethics 23. Biopiracy means (A) Use of biopatents

Answer: (D)

Answer: (C)

Answer: (D)

Answer: (B)

Answer: (C)

Answer: (B)

Answer: (D)

Answer: (C)

Answer: (D)

Answer: (D)

(B) Thefts of plants and animals (C) Stealing of bioresources (D) Exploitation of bioresources without authentic permission 24. Bioethcs is related to (A) Preventing biopiracy (B) Regulation of unethical activities likegene cloning in animals (C) Preventing theft of living materials (D) Moral guidance to the problems in biology 25. Three dimensional shape of tRNA is (A) L-shaped (B) Clover leaf-like (C) X-shaped (D) Y-shaped

Answer: (B)

Answer: (B)

Biotechnology Questions, Paper - 03

Sr. No.
1.

Questions
A DNA nucleotide chain has AGCTTCGA sequence of other chain would be (a) TCGAAGCT (b) GCTAAGCT (c) TAGCATAT (d) GATCCTAG A nucleoside is formed of (a) Pentose sugar, phosphate and nitrogen base (b) phosphate and nitrogen base (c) Pentose sugar and phosphate (d) Pentose sugar and nitrogen base A nucleotide is formed of (a) Purine, Pyrimidine and phosphate (b) Purine, Sugar and phosphate (c) Nitrogen base, Sugar and phosphate (d) Pyrimidine, Sugar and phosphate A riboside is (a) Base + phosphate (b) Ribose + phosphate (c) Ribose + phosphate + base (d) Ribose + base A segment of DNA has 120 adenine and 120 cytosine bases. The total number of nucleotides present in the segment is (a) 120 (b) 240 (c) 60 (d) 480 A Strand of DNA has base sequence CATGACTAG. The base sequence on the other strand would be (a) CAT TAG GAC

Answers
Answer: (a)

2.

Answer: (d)

3.

Answer: (c)

4.

Answer: (d)

5.

Answer: (d)

6.

Answer: (b)

(b) GTA CTG ATC (c) GAT GTC ATC (d) TAC ACT GCT 7. A totipotent cell means (a) An undifferentiated cell capable of developing into a system or entire plant (b) An undifferentiated cell capable of developing into an organ (c) An undifferentiated cell capable of developing into complete embryo (d) Cell which lacks the capability differentiate into an organ or system Adenine is (a) Purine (b) Pyrimidine (c) Nucleoside (d) Nucleotide Amino acid binding site of tRNA is (a) 5end (b) Anticodon loop (c) DHU loop (d) -CCA 3end Anticodon occurs in (a) tRNA (b) mRNA (c) mtRNA (d) rRNA Bacterial plasmid contains (a) RNA (b) RNA + protein (c) DNA (d) Photosynthetic structures Base pairs present in one turn of DNA are (a) 12 (b) 11 (c) 10 (d) 9 Best method to determine paternity is (a) Protein analysis (b) Chromosome counting (c) Gene counting (d) DNA finger printing Callus is (a) Tissue that forms embryo (b) An insoluble carbohdrate (c) Tissue that grows to form embryoid (d) Unorganised actively dividing mass of cells maintained in culture Chemical Knives/ molecular scissors of DNA are (a) Restriction endonucleases (b) Polymerases (c) Ligases (d)Transcriptases Answer: (a)

8.

Answer: (a)

9.

Answer: (d)

10.

Answer: (a)

11.

Answer: (c)

12.

Answer: (c)

13.

Answer: (d)

14.

Answer: (d)

15.

Answer: (a)

16.

Chemofusion and electrofusion are employed in (a) Eugenics (b) Protoplast fusion (c) Cloning (d) Mutations Choose the correct statement (a) DNA is hereditary material (b) RNA is hereditary material (c) DNA is hereditary material but where it is absent RNA can function as hereditary material (d) Both DNA and RNA are hereditary materials Development of shoot and root in tissue culture is determined by (a) Cytokinin and auxin ratio (b) Enzymes (c) Temperature (d) Plant nutrients Distance between two base pairs of DNA is (a) 34 nm (b) 3.4 nm (c) 0.68 nm (d) 0.34 nm Distance between two strands of DNA is (a) 34 (b) 20 (c) 3.4 (d) 340

Answer: (b)

17.

Answer: (c)

18.

Answer: (a)

19.

Answer: (d)

20.

Answer: (b)

Biotechnology Questions, Paper - 04

Sr. No.
1.

Questions
Distance between two successive nitrogenous bases or base pairs of DNA is (a) 34 (b) 3.4 (c) 10 (d) 5 DNA and RNA are similar in having (a) Similar nucleotides (b) Similar pyrimidines (c) Similar purines (d) Similar sugars DNA and RNA show similarity in having (a) Polymers of nucleotides (b) Similar pyrimidines (c) Double strands (d) Similar sugars DNA does not occur in

Answers
Answer: (b)

2.

Answer: (c)

3.

Answer: (a)

4.

Answer: (b)

(a) Nucleus (b) Ribosomes (c) Mitochondria (d) Plastids 5. DNA duplex shows (a) Right handed coiling and parallel (b) Right handed coiling and antiparallel (c) Left handed coiling and antiparallel (d) Left handed coiling and parallel DNA is composed of repeating units of (a) Ribonucleosides (b) Deoxyribonucleosides (c) Ribonucleotides (d) Deoxyribonucleotides DNA replication in eucaryotes commences (a) From both ends of a chromosome simultaneously (b) Several sites along DNA of a chromosome simultaneously (c) From centromere to either end (d) From one end of chromosome to the other DNA replication is (a) Conservative and discontinuous (b) Semiconservative and semidiscontinuous (c) Semiconservative and discontinuous (d) Conservative DNA resembles RNA as both have (a) Polymers of nucleotides (b) Similar sugars (c) Similar pyrimidine bases (d) Ability to replicate DNA sequence is ATG. What would be the sequence of bases in anticodon of tRNA (a) ATG (b) AUG (c) UAC (d) TAC DNA sequence is TAG. What shall be the sequence in anticodon of tRNA (a) UAG (b) ATC (c) ATG (d) UAC DNA sequence of ATTCGATG is transcribed as (a) AUUCGAUG (b) UAAGCUAC (c) CAUCGAAU (d) GUAGCUUA DNA strand with nitrogen base sequence ATTGCC will have sequence in mRNA (a) UAACGC (b) ATCGCC (c) ATTGCA (d) AGGACC Answer: (b)

6.

Answer: (d)

7.

Answer: (b)

8.

Answer: (c)

9.

Answer: (a)

10.

Answer: (b)

11.

Answer: (a)

12.

Answer: (b)

13.

Answer: (a)

14.

Double chained DNA strand is made radioactive in both its chains. It is allowed to replicate twice in non-radioactive medium. The result would be (a) All strands have radioactivity (b) Half the strands have radioactivity (c) Three strands have radioactivity (d) Radioactivity is absent in all strands Double helical structure of DNA was proposed by (a) Kornberg (b) Nirenberg (c) Watson and Crick (d) Wilkins and Franklin Functional unit of gene that specifies synthesis of one polypeptide is (a) Codon (b) Cistron (c) Recon (d) Muton Gene is segment of (a) RNA (b) DNA (c) RNA or DNA (d) Both DNA and RNA Genetically engineered bacteria are being used in commercial production of (a) Melatonin (b) Testoteron (c) Human insuline (d) Thyroxine Haploid plant cultures are got from (a) Leaves (b) Root tip (c) Pollen grain (d) Buds Hydrogen bonds present between cytosine and guanosine are (a) 2 (b) 3 (c) 1 (d) 4

Answer: (b)

15.

Answer: (c)

16.

Answer: (b)

17.

Answer: (b)

18.

Answer: (c)

19.

Answer: (c)

20.

Answer: (b)

Biotechnology Questions, Paper - 05

Sr. No.
1.

Questions
In a DNA molecule cytosine is 18%. Percentage of adenine would be (a) 32% (b) 64% (c) 36% (d) 18% In AGCT of DNA hydrogen bonds and base pairings occur between

Answers
Answer: (a)

2.

Answer: (d)

(a) A-U, C-G (b) A-C, G-T (c) A-G,C-T (d) A-T,C-G 3. In callus culture, roots can be induced by the supply of (a) Auxin and no cytokinin (b) Higher concentration of auxin and lower concentration of cytokinin (c) Higher concentration of cytokinin and lower concentration of auxin (d) Both auxin and cytokinin in equal proportions. In DNA, adenine pairs with (a) Guanine (b) Thymine (c) Cytosine (d) Uracil In DNA, guanine lies opposite (a) Uracil (b) Cytosine (c) Adenine (d) thymine In double helix of DNA, the two DNA strands are (a) Coiled around a common axis (b) coiled around each other (c) coiled differently (d) Colied over protein sheath In RNA, thymine is replaced by (a) Adenine (b) Guanine (c) Cytosine (d) Uracil In tissue / bacterial culture glassware and nutrients are sterilized through (a) Water bath at 200 C (b) Dry air oven at 200 C (c) Dehumidifire (d) Autoclave In tissue culture, callus can be induced to form shoot or root by altering the ratio of (a) Auxin to cytokinin (b) Cytokinin to ethylene (c) Auxin to gibberellin (d) Gibberellin to cytokinin Initiation codon of protein synthesis (in eucaryotes) is (a) GUA (b) GCA (c) CCA (d) AUG Introduction of foreign genes for improving genotype is (a) Tissue culture (b) Immunisation (c) Biotechnology (d) Genetic engineering It is now possible to breed plants and animals with desired characters through Answer: (b)

4.

Answer: (b)

5.

Answer: (b)

6.

Answer: (a)

7.

Answer: (d)

8.

Answer: (d)

9.

Answer: (a)

10.

Answer: (d)

11.

Answer: (b)

12.

Answer: (a)

(a) Genetic engineering (b) Chromosome engineering (c) Ikebana technique (d) Bonsia technique 13. Most abundant RNA of the cell of (a) tRNA (b) rRNA (c) mRNA (d) tRNA Nitrogen bases of DNA are (a) ATUC (b) UTGC (c) ATGC (d) AUGC Nonsense codon takes part in (a) Terminating message of gene controlled protein synthesis (b) Formation of unspecified amino acids (c) Conversion of sense DNA into non-sense one (d) Releasing tRNA from polypeptide chain Nucleosides are (a) Sugar + Phosphoric acid (b) Purine / Pyrimidine + Sugar + Phosphate (c) Purine / Pyrimidine + Phosphoric acid (d) Purine / Pyrimidine + Sugar Nucleotides present in one turn of DNA helix (a) 4 (b) 8 (c) 10 (d) 9 Okazaki segments are formed during (a) Transduction (b) Transcription (c) Replication (d) Translation Plants developed in vitro culture from pollen grains are (a) Androgenic plants (b) Pollen plants (c) Male plants (d) Sterile plants Plasmids are vectors for gene cloning because they (a) Self replicate in bacterial cells (b) Replicate freely outside bacterial cells (c) Can be multiplied in culture (d) Can be multiplied in laboratories using enzymes Answer: (b)

14.

Answer: (c)

15.

Answer: (a)

16.

Answer: (d)

17.

Answer: (c)

18.

Answer: (c)

19.

Answer: (a)

20.

Answer: (a)

Biotechnology Questions, Paper - 06

Sr. No.
1.

Questions
Pomato is somatic (a) Poppy and Potato (b) Potato and tomato (c) Poppy and tamarind (d) Poppy and Tomato Preserving germplasm in frozen state is (a) Cryopreservation (b) Cold storage (c) In situ preservation (d) Vernalisation Protein helping in opening of DNA double helix in front of replication fork is (a) DNA gyrase (b) DNA Polymerase I (c) DNA ligase (d) DNA topoisomerase Pyrimidine base present in RNA in place of thymine of DNA is (a) Uracil (b) Adenine (c) Cytosine (d) Guanine Restriction endonuclease is employed for cutting (a) A single stranded DNA (b) Double stranded DNA (c) RNA fragment (d) mRNA Restriction endonucleases are (a) Used in genetic engineering for uniting two DNA molecules (b) Used for in vitro DNA synthesis (c) Present in mammalian cells for degeneration of DNA of dead cells (d) Synthesised by bacteria for their defence Restriction enzymes are used in genetic engineering because they (a) Can join DNA fragment (b) Cut DNA at specific base sequence (c) Cut DNA at variable sites (d) Are proteolytic enzymes which degrade harmful proteins Reverse transcriptase is (a) RNA dependent RNA polymerase (b) DNA dependent RNA polymerase (c) DNA dependent DNA polymerase (d) RNA dependent DNA polymerase RNA contains (a) Hexose (b) Ribose (c) Fructose

Answers
Answer: (b)

2.

Answer: (a)

3.

Answer: (a)

4.

Answer: (a)

5.

Answer: (b)

6.

Answer: (d)

7.

Answer: (d)

8.

Answer: (d)

9.

Answer: (b)

(d) Glucose 10. RNA does not possess (a) Uracil (b) Thymine (c) Adenine (d) Cytosine RNA that picks up specific amino acid from amino acid pool of cytoplasm to carry it to ribosome during protein synthesis is (a) tRNA (b) mRNA (c) rRNA (d) gRNA Semiconservative DNA / Chromosome replication using 14N was demonstrated by (a) Messelson (b) Tylor (c) Messelson and stahl (d) Hershey and Chase Similarity between DNA and RNA is that both have (a) Similar sugars (b) Similar mode of replication (c) Similar pyrimidines (d) Polymers of nucleotides Structure of DNA was given by (a) Kornberg (b) Nirenberg (c) Watson and Crick (d) Holley and Nirenberg Successive nucleotides are covalently linked through (a) Glycosidic bonds (b) Phosphodiester bonds (c) Hydrogen bonds (d) Nitrogen bonds Termination of polypeptide chain is brought about by (a) UUG, UAG and UCG (b) UAA, UAG and UGA (c) UUG, UGC and UCA (d) UCG, GCG and ACC The basic uniot of a nucleic acid is (a) Pentose sugar (b) Nucleoid (c) Nucleoside (d) Nucleotide The codon for anticodon 3 UUUA - 5 is (a) 5 AAAU- 3 (b) 5 UUUA- 3 (c) 3 UAAD- 5 (d) 3 AUUU- 5 Answer: (b)

11.

Answer: (a)

12.

Answer: (c)

13.

Answer: (d)

14.

Answer: (c)

15.

Answer: (b)

16.

Answer: (b)

17.

Answer: (d)

18.

Answer: (a)

19.

The common feature amongst nucleus, chloroplast and mitochondria is (a) Lamellae (b) DNA (c) Cristae (d) All the above The enzyme taking part in joining two ends of DNA is (a) Ligase (b) Polymerase (c) Gyrase (d) Helicase

Answer: (b)

20.

Answer: (a)

CHAPTER 9 1. Genetic engineering is the manipulation of ________ for practical purpose.

Genetic bacteria Genetic plant Genetic material Genetic animal

2. The ________ technology has launched an industrial revolution in bio-technology.

DNA transfer DNA recombinant genetic bacteria genetic transferring

3. Recent reproductive cloning intended to introduce ________.

identical plants genetic bacteria identical animals identical sheeps

4. ________ was the first ever cloned animal.

Dolly Tomy Kitty Spike

5. ________ formulated rule to explain the phenomenon of inheritance.

Gregor Johann Mendel Charles Darwin Jean Baptiste de Lamark All of them

6. Each basis inheritable character is controlled by a factor called ________.

Chromosome DNA RNA gene

7. Gene is made up of ________.

Proteins RNA DNA All of them

8. The discovery of ________ was a tremendous stimulus to genetic research.

DNA of bacteria cloned Dolly Role of DNA genetic engineering

9. Methodology was discovered in ________.

1952 1956 1973 1989

10. Methodological methods were referd to as ________.

Recombinant DNA technology Genetic engineering Both All of them

11. ________ is defined as the se of living organisms for the welfare of mankind.

Micro-biology Human biology Bbiotechnology Zoology

12. Genetic engineering usually utilizes ________.

Bacterial cells

Bacterial plasmids Both of them None of them

13. Recombinant DNA technology depends on ________ steps.

3 4 5 8

14. A gene is inserted into a DNA molecule called ________.

vector pasmid both none of them

15. ________ are small, extra circular DNAs molecules found in some bacteria.

vector plasmid chromosome genetic engineered DNA

16. Plasmid replicated ________ of the host cell.

dependent independent mostly dependent mostly independent

17. Plasmids are generally found in ________.

bacteria vertebrates all living organisms bacteriophages

18. The group of enzymes that are used to cut up the DNA molecule are called ________.

restriction enzymes DNA ligase enzyme DNA polymerase enzyme none of them

19. ________ protest bacteria against bacteriophage by cutting viral DNA.

DNA polymerase enzyme restriction enzyme

DNA ligase enzyme all of them

20. The molecular scissors in the bacterial cell are ________.

DNA ligase enzyme vector plasmid restriction enzyme

21. Each restriction enzyme cut the DNA at a special site called ________.

sticky end site of attachment cutting end joining ends

22. ________ is a key enzyme that seals the restriction fragment with sticky ends of vector.

DNA polymerase enzyme DNA ligase enzyme Restriction enzyme A and B

23. ________ are taken as host in DNA recombinant technology.

bacterial cells vectors plasmids bacteriophage

24. The gene carried by recombinant molecule is called ________.

cloned copied multiplied engineered

25. ________ is used to form genetically engineered plants which are resistant towards insects.

genetically engineered bacteria vector plasmid all of them

26. Bacteria which usually live in the roots of corn are transformed for producing ________.

good roots strong barks insect killing foxins

healthy fruits

27. Hormones can also be prepared by ________.

cloned bacteria plasmid vector rDNA

28. Phynylalanine is used to make a sweetener called ________.

phenyl sweets nutra sweets cemilky sweets natural sweets

29. When a foreign gene is inserted to natural plants they are called ________.

Transgenic plants genetically engineered plants both A and B all of them

30. In ________ technique entire organism can be grown from a single call.

tissue culture cloning genetic engineering transfusion

31. When the cell wall of a plant cell is removed it is called ________.

protoplast plant cell changed cell transgenic cell

32. In plant cell the rDNA can be introduced into ________.

Enbryo Protoplast Cell having cell wall Both A and B

33. The only plasmid for transgenic plants is ________.

Pr-plasmid Ti-plasmid Ds-plasmid All of them

34. ________ is the host for the host for the Ti-plasmid.

Bacteriophage Agrobacterium Phenylalamine All bacteria

35. Transgenic plants are able to make use of ________ of the atmosphere so they dont require fertilizers.

Oxygen Nitrogen CO2 All of them

36. ________ is the fastest way of determining amino acid sequences.

DNA finger printing Gene sequencing Genomic library Human genome project

37. ________ provides the location of Restriction site within the gene.

Gene sequencing DNA finger printing Genomic library All of them

38. The similarity in the DNAs of two different organisms can be determined and studied by ________.

DNA finger printing Human genome project Gene sequencing Gene therapy

39. ________ can separate DNA fragments differing in as much as 1 segment in gene sequencing.

Gel electrophoreses DNA electrophoreses Gene electrophoreses All of them

40. ________% of DNA does not code for proteins

20% 30% 40% 50%

41. The difference in DNA electrophoresis patterns among individuals is called ________.

DNA finger prints DNA fragment length DNA length measurement Restriction fragment length polymorphism

42. DNA finger prints is named so due to ________

RFLPs length Sequence of RFLPs is unique for each Both A and B None of the above

43. To produce a DNA finger print ________ would suffice.

A sample of blood A sample of saliva Sample of hair folicle All of them

44. ________ consists of the copy of genetic information or a specre the prefered environment.

Genome library DNA finger prints DNA fragment Gene sequencing

45. Human Genome Project began in ________.

1990 1980 1994 1991

46. Total number of chromosome pairs inhuman is ________.

23 26 44 46

47. ________% of the DNA sequence have determined with 99% accuracy.

89% 95.658% 99.335% 99.99%

48. ________% of the nucleotide sequence in all the hamans is same.

50%

86% 99% 99.9%

49. Medical researches have discovered about ________ genetic diseases.

3000 4000 5000 6000

50. Genetic diseases is mostly related with ________.

Sperms Ova Fertilized egg or zygote All of them

51. There are ________ conditions for the occurance of genetic diseases.

2 3 5 9

52. Is mutation a cause of genetic diseases?

Yes always No May be Not involved at all

53. Genetic diseases fall into ________ main categories.

2 3 5 8

54. Following is the category of genetic diseases.

Chromosomal abnormalities Unifactorial defects Multifactorial defects All of them

55. Huntingtons disease is due to a ________.

Autosomal resesive allele Autosomal dominant allele

Hetyerozygous allele Homozyhous allele

56. In huntingtons disease the symptoms appear by the age of ________.

35-45 40-50 55-65 50-60

57. Progressive mental deterioration and unvoluntary muscle movement is the characteristic of ________.

Chromosomal disorder Chromosomal disjunction Huntingtons disease Cystic fibrosis

58. Is a cure for huntingtons disease possible.

Yes May be Depends on nature No

59. Cystic fibrosis occurs due to ________

Autosomal Recessive Allele A heterotroph Homozygous Chromosome Dominant Allele

60. ________ is a diagnostic process in which man amount of amniotic fluid is taken from the amniotic sac to determine the presence of disease.

Genetic councelling Tissue culture Gene therapy Amniocentesis

61. Through ________ any disease can be determined in the embryonic stage.

Amniocentesis Amniotic tissue test fefune blood test Gene therapy

62. ________ is a test tube method to create and olone various varieties of novel plants.

Cloning Genetic engineering

Gene therapy Tissue culture

63. The cell which contains all the genetic potential of the organism is called ________.

Potitotent Totipotent Sturgid call Complete cell

64. Through cloning the duplicate coples of ________ can be formed.

Genetic material Cells Both of them None of them

65. Artificial cloning is now possible by the pethology ________.

Recombinant DNA technology Gene therapy DNA finger prints Genomic library

66. The copies of individuals formed by clonig are called ________.

Twins Identicals Clones Copies

67. In 1997, a ________ was cloned from cell from its mother.

Cat Dog Fish Sheep

68. The biotechnology is now also being considered dangerous due to

Its useful cloning method DNA finger printing Harmful side effects Artificial Techniques

69. In gene therapy of germ line cells the recombinant DNA is inserted into ________.

Human sex cells Fertilized zygote Somatic cells

All of them