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    Mixing in fermentation broths is important to dissolve solids, disperse air bubbles, improve heat transfer, and allow for even distribution of nutrients and substrates. This provides an environment conducive for cell suspension growth. Common mixing equipment includes impellers, turbines, and propellers. Factors that affect the selection of mixing equipment include cell morphology, susceptibility to shearing, viscosity, density, and power requirements. Power requirements are a function of viscosity, density, suspended solids, agitator speed, impeller type, and orientation. For ungassed and Newtonian systems, relationships between these variables are expressed using dimensionless numbers like power, Reynolds, and Froude numbers. For non-Newtonian fluids,

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    Mixing in fermentation broths is important to dissolve solids, disperse air bubbles, improve heat transfer, and allow for even distribution of nutrients and substrates. This provides an environment conducive for cell suspension growth. Common mixing equipment includes impellers, turbines, and propellers. Factors that affect the selection of mixing equipment include cell morphology, susceptibility to shearing, viscosity, density, and power requirements. Power requirements are a function of viscosity, density, suspended solids, agitator speed, impeller type, and orientation. For ungassed and Newtonian systems, relationships between these variables are expressed using dimensionless numbers like power, Reynolds, and Froude numbers. For non-Newtonian fluids,

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    Attribution Non-Commercial (BY-NC)
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    85 views18 pages

    Mixing in Fermentation Broth

    Uploaded by

    taniadey01
    Mixing in fermentation broths is important to dissolve solids, disperse air bubbles, improve heat transfer, and allow for even distribution of nutrients and substrates. This provides an environment conducive for cell suspension growth. Common mixing equipment includes impellers, turbines, and propellers. Factors that affect the selection of mixing equipment include cell morphology, susceptibility to shearing, viscosity, density, and power requirements. Power requirements are a function of viscosity, density, suspended solids, agitator speed, impeller type, and orientation. For ungassed and Newtonian systems, relationships between these variables are expressed using dimensionless numbers like power, Reynolds, and Froude numbers. For non-Newtonian fluids,

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    Mixing in FERMENTATION BROTH

    Tania Dey B090918Ch

    What is mixing ?
    Mixing, a physical process which aims at reducing non-uniformities in fluids by eliminating gradients of concentration, temperature, and other properties.

    What is fermentation broth ?


    Medium Gas bubbles Substrate

    Nutrients

    Cells in suspension

    Importance..
    Dissolve solids in liquids Dispersing air in form of bubbles Improve heat transfer Consumption of oxygen Increases interfacial area Equilibrate the conc of nutrients and substrate Overall provides conductive environment for cell suspention to interact with the sustrate in a better manner.

    Mixing equipment (Standard mixing tank)


    Impeller Turbine Propeller

    Various types of Agitator

    Factors affecting selection of mixing equipment


    Cell morphology Susceptibilty of cell to shearing Viscosity Density Interphase (gas-liquid/ liquid -liquid) Power requirement

    Power requirement for mixing


    Electrical power required to drive the motor connected to agitator. Is a function of
    a) b) c) d) e) f) Viscosity Density Presence of suspended solids Speed of agitator Type of impeller Orientation of vanes

    Ungassed Newtonian fluids


    The relationship between variables affecting power required is usually expressed in terms of dimensionless numberspower number(Npo) Impeller Reynolds number(NRei) Froude number(NFr) Po - power required Ni - impeller speed di - impeller diameter

    Froude number takes care of gravitational effect Only significant for unbaffled fermentor and cases where NRei <300 Power number gets modified to-

    NRei versus Npo for various impellers

    Zone 1
    LAMINAR ZONE Npo falls as NRei increases

    Zone 2
    TRANSITION ZONE In which the plots are curved

    Zone 3

    TURBULENT ZONE Npo is constant as NRei increases

    Ungassed Non Newtonian fluids


    Difficult as viscosity changes due to shear rate Seldom reach turbulent zone Apparent viscosity [a] is used in Nrei a = K-1

    Nrei(non newtonian)= (Nidi2)/(K-1)

    Ungassed tank with ruston turbine

    Gassed system
    Power required in gasses is always less than ungassed .

    Pg power required for gassed system Fg volumetric gas flow rate V liquid volume Wi width of the impeller

    Na is aeration factor given by Na = Fg/(Nidi3)

    Thank you!!!

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