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OWLS APPLICATION NOTES NO-003

LABEL-FREE IMMUNOSENSOR
FOR ESCHERICHIA COLI DETECTION
using OPTICAL WAVEGUIDE LIGHTMODE SPECTROSCOPY (OWLS) detection

Abstract
Escherichia Coli is a Gram-negative non-spore forming rod and a representative microorganism in the enteric bacteria. It is also
considered as index microorganism in the food industry. OWLS immunosensor offers a real time label-free detection of E.Coli
bacteria For biosensing monoclonal antibodies raised against E.Coli bacteria. To form regenerable sensor surface, the
waveguide sensor surface was modified with amino group and sensitized by immobilizing antibody/antigen molecules covalently
to the surface.

Application of OWLS sensors


as immunosensor for the detection of E.Coli Calibration curve for E.Coli sensor
Concentration dependent response of E.Coli by
E. coli OW LS
sample
monoclonal antibody

100 - 150 nm

Laser light waveguide

EM field glass
distribution support

Surface Chemistry
• Amino fictionalization of waveguide surface by
(γ-aminopropyl)triethoxysilane
• Immobilization of biomolecules on the amino
surface of the OW LS sensors by
glutaraldehyde.

Sensitization of the sensor surface Conclusion


with monochlonal anti-E.Coli The OW LS Sensor is a rapi d and cost-effective tool
for Escherichia Coli detection.
650 anti-E.Coli. 100x
W ith the pres ented immunosensor the lowest
detectable amount of E. Coli is 3x103 CFU/ml.
S e nsor re sponse (a. u.)

550 HCl, 10 mM

450 GA TRIS

350
References
250
(d.w. 1. Vörös, J. J. Ramsden, G. Csucs, I. Szendrõ,
150 S . M . D e P a u l , M . T e xt o r , N . D . S p e n c e r
0 10 20 30 40 50 “Optical Grating Coupler Biosensors”
Time (min) Biomaterials 23 (2002) 3699-3710

2. Adányi N. Váradi M., Kim N., Szendrı I.


“Development of new immunosensors for
Regeneration cycles of the measurement determination of contaminants in food” Current
Antibodies are covalently attached to the surface Applied Physics ( in press)
a n d l i f e t i m e o f s e n s o r s we r e e x a m i n e d a n d
optimized. 3. w w w . o wl s - s e n s o r s . c o m

560 3E+7
CFU/ml
540
3E+6
(a. u.)

520
response unit)

500
3E+5
3E+3
Mass (arbitrary

480

460
Sensor

440

420

400

380

60 80 100 120

Time (min)

MicroVacuum Ltd. H-1147, Kerékgyártó u. 10., Budapest, Hungary Phone: + 36 1 252 1991; +36 1 467 0108 Fax: +36 1 221 7996
Web: http://www.owls-sensors.com/; E-mail: info@owls-sensors.com Copyright @ Microvacuum, 2006

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