Mice preparation First I need to collect Ox2Rf/+ mice. Than mating within them.

After some generation I will get Ox2Rf/f mice. Then again I will mat with Ox2Rf/f and Gad 1 Cre mice. After some generation I will get Ox2Rf/+: Gad 1 CreTg/+ mice. Again I need to mating Ox2Rf/+: Gad 1 CreTg/+ mice with Ox2Rf/f or Ox2Rf/+ mice. Finally I will get Ox2Rf/f: Gad 1 CreTg/+ mice.

Ox2Rf/+ ---Orexin 2R Floxed Hetero mice Ox2Rf/f--- Orexin 2R Floxed Homo mice

Ox2Rf/+ X Ox2Rf/+ Ox2Rf/f Ox2Rf/f X Gad 1 cre Ox2Rf/+: Gad 1 CreTg/+ Ox2Rf/+: Gad 1 CreTg/+ X Ox2Rf/f or Ox2Rf/+ Ox2Rf/f: Gad 1 CreTg/+