You are on page 1of 42

1

Atsuo Yanagisawa, M.D., Ph.D.


Japanese College of IV Therapy
Effect of Vitamin C and anti-
oxidative nutrition on radiation-
induced gene expression in
Fukushima nuclear plant workers
=20
=80
There are many scientific studies
demonstrate protective effects of
Vitamin C upon radiation-induced
cellular injury .
!"#$%&'()*+,A nuc|ear power p|ant acc|dent
occurred |n 1999 |n Iapan. anents who were exposed
to h|gh doses of rad|anon deve|oped severe bone
marrow fa||ure, and thereaher underwent stem ce||
transp|antanon. nowever, they deve|oped severe GI
damage w|th d|arrhea and b|eed|ng, and eventua||y
d|ed from mu|np|e organ fa||ure.
-'./'.#/0.*/123/4156$('73$15$3+1-'.8.*/61
9./4#:1;#6/'(3*/.6<*#:1=>*+'(0.13*1?3$.1
@.$.383*&1#1?#6638.150()*/1(A1@#+3#<(*B1
!"#$%%
&'(#)'*%+,-,).,%/,012'*%3#**,4,%5%%
6"#7)0%8,*-9%+,-,).,%!#"2,%1)%:';')%%
<'$'$#=#%,=%'*>%:%?'01'=%?,.%CDED@ABCBDA9BA>%
#1: V|tam|n C |s a protector aga|nst externa| rad|anon
T. Yamamoto et al. 148
al after 14 Gy WBI. We preliminarily studied dose response
curve of treatment with ascorbic acid in the irradiated mice.
The mice were pretreated with 1.5, 15, 150, and 1,500 mg/
kg/day of ascorbic acid for 3 days before 14 Gy WBI. They
then received BMT 24 h after radiation. Pretreatment with
150 mg/kg/day of ascorbic acid rescued some subjected
mice, although pretreatment with other doses of ascorbic
acid rescued no mice (data not shown), thereby studying 150
mg/kg/day of ascorbic acid in the following experiments.
Oral intake of 1,500 mg/kg/day ascorbic acid induced potent
Fig. 1. Mouse survival after whole body radiation (A). Mice were irradiated at 6, 8, 10, 12, and 14 Gy. The
effect of bone marrow transplantation on mouse survival after radiation (B). Mice were irradiated at 10, 12,
and 14 Gy. Mice received BMT 24 h after radiation. N = 12 in each group. *P < 0.01 vs. other groups.
Fig. 2. Radiation-induced damage to the bone marrow and small intestine in mice 7 days after radiation. The mice were irradiated at 0, 8,
and 14 Gy. Seven days after whole body radiation, they were euthanized to remove the bone marrow and small intestine, and radiation-
induced damage to these organs was examined. Arrows indicate severe radiation-induced degeneration of the small intestine (C-b). Images
shown are representative of each mouse group (n = 5).
Mouse cou|d surv|ve |ess than than 8 Gy who|e body |rrad|anon
(W8I). 8one marrow transp|antanon (8M1) 24 h aher |rrad|anon
rescued m|ce rece|v|ng |ess than 12 Gy. nowever, no m|ce rece|v|ng
14 Gy-W8I surv|ved, because of rad|anon-|nduced GI syndrome.
amamoto et a|. I kad|at kes 2010,S1:14S-1S6.
Prevention of Radiation GI Syndrome by Ascorbic Acid 149
diuretic effect in mice, leading to severe dehydration and
thereby exacerbating mortality. Next, we examined the effect
of pretreatment or post-treatment with ascorbic acid on the
irradiated mice. Mice were treated with 150 mg/kg/day of
ascorbic acid for 3 days before or after 14 Gy WBI and they
also received BMT at 24 h after radiation. Pretreatment with
ascorbic acid before 14 Gy WBI dramatically improved
mouse survival (42% survival), whereas treatment with
ascorbic acid after radiation did not affect survival (no mice
survived) (Fig. 3). When the mice did not receive BMT fol-
lowing 14 Gy WBI, pretreatment with ascorbic acid rescued
no subjected mice (Fig. 3). The rescued mice that were pre-
treated with ascorbic acid and received BMT after 14 Gy
WBI were healthy 30 days after radiation, although they
showed marked radiation-induced depigmentation in the fur
(data not shown).
Fig. 3. The effect of treatment with ascorbic acid on mouse survival after radiation. The mice were irradi-
ated at 14 Gy and received BMT 24 h after radiation. They received ascorbic acid p.o. for 3 days either before
or after radiation, or received distilled water only before radiation. The mice pretreated with ascorbic acid
were also irradiated at 14 Gy without BMT. N = 12 in each group, *P < 0.01 vs other groups.
Fig. 4. The effect of pretreatment with ascorbic acid or BMT following WBI on the bone marrow or small intestine in the irradiated mice.
The mice were pretreated with ascorbic acid p.o. or distilled water for 3 days and then irradiated at 14 Gy. One day after radiation, they
received BMT or did not. The mice were euthanized 7 days after radiation to assess radiation-induced damage to the bone marrow and small
intestine. Data shown are representative of each mouse group (n = 5).
V|tam|n C FEGD10&H%&H+#>I1was ora||y adm|n|stered for 3 days,
and then the m|ce underwent 14 Gy |rrad|anon. retreatment
w|th ascorb|c ac|d marked|y |mproved rad|anon-|nduced
|ntesnna| damage, thereby rescu|ng m|ce rece|v|ng W8I at 14
Gy |n comb|nanon w|th 8M1.
12
J*1?#'$41EKL1CDEEL1/4.1'.6$).1/.#01(A1M#<(*#:1=.:AN
O.A.*6.1P('$.1/((%1QJR5?JM1S124.*1/4.>12.'.113*1
/4.1P)%)6430#1M)$:.#'1-:#*/B1
"When we undertake the rescue of v|cnms from a
rad|anon-contam|nated area [ust aher a rad|anon
acc|dent or terror|sm, |t |s |mportant for rescue team
members to T'(0T/:>1/#%.1Q3/#03*1S1ora||y."
5)/4('61(A1M#<(*#:1O.A.*6.1
?.+3$#:1S(::.&.1U1;'()*+11
=.:AN1O.A.*6.1P('$.13*1V#T#*1
$(00.*/.+13*1/4361#'<$:.L1
Intra-tesncu|ar |n[ecnon of Iod|ne-131 decreased sperm
head surv|va| |n m|ce and |s known as an exper|menta|
mode| of |nterna| rad|anon |n[ury.
retreatment of |ntra-tesncu|ar |n[ecnon of V|tam|n C or
ora| |ntake of V|tam|n C |ncreased sperm head surv|va| aher
the |n[ecnon of Iod|ne-131 |n m|ce.
1hus, v|tam|n C |s a rad|oprotector when rad|onuc||des are
|ncorporated |n the body and de||ver the dose |n a chron|c
fash|on.
Q3/#03*1S1#61#1@#+3(T'(/.$/('11
11111115&#3*6/1J(+3*.NEKE1J*1Q38(1
M#''#1Q@1./1#:B1V1M)$J?.+1EWWKXKYZ[K\N[YD
#2: V|tam|n C |s a protector aga|nst |nterna| rad|anon
8|ood samp|es were taken before and 1 h aher breakfast
w|th ora| |ngesnon of v|tam|n C (3S mg]kg).
Samp|es were |mmed|ate|y |rrad|ated us|ng a Coba|t-60
hotspot and DNA damage was measured us|ng the 'comet'
assay.
V|tam|n C |ngesnon showed a s|gn|hcant reducnon |n DNA
damage due to |rrad|anon and |ts peak eect was 4 hour
aher |ntake of v|tam|n C.
]^.$/1(A1+3./1#*+183/#03*1S1(*1OM516/'#*+1
7'.#%#&.13*1A'.64:>N36(:#/.+14)0#*1243/.1
7:((+1$.::6B1
;'..*1?_91./1#:B1?)/#<(*1@.6.#'$41KE[FEWWYIWENEDC
#3: Cra| |ntake of v|tam|n C prevent rad|anon-
|nduced DNA damage |n humans.
Cra| |ntake of a|pha-||po|c ac|d and v|tam|n L
reduced ur|nary rad|oacnv|ty and ox|danve
stress |n |rrad|ated ch||dren |n Chernoby|.
1here are numerous sc|ennhc reports about
nutr|non as a rad|o-protector such as V|tam|n A,
C, L, k, Se|en|um, G|utath|one, Me|aton|n,
prob|oncs, kesveratro|, etc.
Ann-ox|danve reserve of the body |s a key ro|e
for prevennng rad|anon |n[ury.
kad|anon |n[ury can be protected
by ann-ox|danve nutr|nons
`('%3*&1;'()T1(A1R4.1=/#/.0.*/1
5/6)(1a#*#&36#2#1?BOB 1 1?#6#6431b2#7)L1?BOB1
")'/(*1]B1")'%6(*L1?BOB11 1"'#+A('+1=B1`..%6L1?BOB11
@(*#:+1_)**3*&4#%.L1?BOB1 1=/.8.*1_3$%.>L1-4BOB1
R4(0#619.8>L1?BOB1
It is our strongest recommendation that those
living in the effected areas regularly take
antioxidant supplements such as vitamin C to
counteract the negative consequences of long-
term low dose radiation exposure as well as to
protect the health of coming generations.
Cmc|a| Statetment of ICI1 about
"Lnv|ronmenta| rad|oacnv|ty and hea|th"
|ssued |n March 29, 2011.
Dr. kon nunn|nghake Dr. Steve n|ckeyDr. Atsuo anag|sawa
Dr. 1homas L. Levy Dr. 8urton M. 8erkson Dr. 8radford S. Weeks Dr.Masash| Uwabu
-.(T:.124(14#8.1#1T(6637:.13*/.'*#:1#*+H('1
.c/.'*#:1'#+3#<(*1.cT(6)'.164():+1/#%.1#*<N
(c3+#<8.16)TT:.0.*/61/(10#3*/#3*1(T<0#:1#*<N
(c3+#<8.1'.6.'8.1(*1/4.3'1(2*17(+>B1
V|tam|n C 1-3g 3-4 nmes a day
or L|posoma| V|tam|n C 1-2 g tw|ce a day
A|pha-||po|c ac|d 100-300 mg tw|ce a day
Se|en|um S0-200g tw|ce a day
V|tam|n L 100-200 mg tw|ce a day
w|th other essenna| v|tam|ns and m|nera|s.
ICI1 - kecommendanon
(1) V|tam|n C 2Sg |.v. before work|ng at nuc|ear p|ant.
rad|anon-contam|nated area.
Ster||e water 2S0mL S0 v|tam|n C S0mL
20 Mg-ch|or|de SmL 1 812 1mL
8-comp|ex 100 1mL 10 86 1mL
2SDexpantheno| 1mL
(2) Da||y supp|ements at work
L|posoma| V|tam|n C 2 g < three nmes a day
A|pha-||po|c ac|d 300 mg < tw|ce a day
Se|en|um 200 g < tw|ce a day
V|tam|n L 200 mg < tw|ce a day
w|th other essenna| v|tam|ns.
ICI1 recommendanon for peop|e who works at
rad|anon-contam|nated area |n the Iuk|sh|ma
Nuc|ear |ant.
Name|ess
Iace|ess
neroes
Japanese College of
Intravenous Therapy
(JCIT)
We are 400 members: 379 physicians
12 dentists
9 veterinarians
More than 10 educational seminars every year.
IV Vitamin C, IV Lipoic acid, IV Chelation,
Myers Cocktail, Ukrain, Nutrition therapy, etc.
24
23
The 1
st
JCIT Symposium of IVC for Cancer Patients
Oct 17, 2010, Tokyo, Japan
26
More Lhan 170 !Cl1 member
cllnlcs oer radlo-proLecuve
medlcal consulLauon Lo
Lhe !apanese cluzen along
Lhe !Cl1 sLaLemenL.
27
Effect of Vitamin C and anti-
oxidative nutrition on radiation-
induced gene expression in
Fukushima nuclear plant workers
- A pilot study
Atsuo YANAGISAWA, M.D., Ph.D.
(1)

Michie IWATA, M.D., Ph.D.
(2)

Shuichi AKIYAMA
(3)
(1) Japanese College of IV Therapy
(2) Iwata Ladies Clinic,
(3) Gene Science Co, Ltd.
Introducnon
In the statement of Environmental Radioactivity and Health
issued in March 29, 2011, we strongly recommended that
People living in the effected areas should regularly take
antioxidant supplements such as vitamin C to counteract the
negative consequences of long-term low dose radiation
exposure as well as to protect the health of coming
generations. However, TEPCO, government and all medias
ignored our statement.
Recently, we examined cancer-related gene expression in 16
men who had worked at severe radiation environment at
Fukushima nuclear plant. We also confirmed the improvement
of cancer risk score by the supplementation of Vitamin C and
other anti-oxidative nutrition.
28
Methods (1) Study sub[ects
Sub[ects:
16 men (32-S9 yrs.) who worked S-6 weeks at
rad|anon contam|nated area of Iukush|ma Nuc|ear
|ant aher March 12, 2011.
A|| sub[ects were temporary worker of sub-sub
contractor company of 1LCC.
Work operanons at contam|nated area:
--> Co||ecnng contam|nated water
--> Measur|ng rad|anon |eve| |n the p|ant area
--> kemova| of debr|s
--> Cperanon of heavy mach|nery
29
Methods (2) Measurement
8|ood samp|e was obta|ned few days aher the
work|ng per|od. In 4 workers, b|ood was obta|ned
at before and aher the work|ng per|od.
Measurement:
(1) Who|e b|ood counts and b|ood chem|stry
(2) |asma |eve|s of free DNA
(3) 47 cancer re|ated gene express|on
(4) Ca|cu|ate "Cancer k|sk Score"
"Cancer k|sk Score" was ca|cu|ated us|ng equanon
formed by the |og|snc ana|ys|s from the va|ue of
47 cancer-re|ated gene express|ons.
30
Methods (3) Intervennon
31
(1) Intravenous V|tam|n C 2Sg prescr|pnon
Ster||e water 2S0mL 12.S MgSC4 SmL
S0 v|tam|n C S0mL (2Sg)
V-81: 120mg V-82: 2mg V-83 : 40mg
V-8S: 2S4mg V-86: 120mg V-812: 1mg
(2) Da||y ora| supp|ements
Lyposoma| V|tam|n C 1 g tw|ce a day
A|pha-||po|c ac|d 300 mg tw|ce a day
Se|en|um 200 g tw|ce a day
V|tam|n L 200 mg tw|ce a day
Mu|n-V|tam|ns tw|ce a day
F Concentration
Cut-off value <25ng/mL
>25ng/mL
Healthy control 17% (n=400)
Cancer patients 38% (n=400)
F
r
e
e

D
N
A

c
o
n
c
e
n
t
r
a
t
i
o
n

(
n
g
/
m
L

p
l
a
s
m
a
)

0
25
50
75
100
125
150
175
200
32
D|str|bunon of Cancer k|sk Score on
nea|thy sub[ects and Cancer anents.
0
5
10
15
20
25
10 20 30 40 50 60 70 80 90 100

0100

33
Cancer 8lsk Score (0-100)
cancer pauenLs
healLhy sub[ecLs
23
20
13
10
3
0

Cancer Risk Score is calculated from the value of 48 cancer-


related gene expression using logistic analysis.
34
Plasma F
ng/mL
|asma Iree DNA and Cancer k|sk Score
|n Iukush|ma Workers
38
0

Cancer Risk Score


23
0
Five workers were treated with IVC twice a month and oral anti-oxidative
nutritional supplements.
35
<0.03
ng/mL
F
Cancer Risk Score
Before 2 M Before 2 M
Lect of ann-ox|danve nutr|nona| |ntervennon on Iree
DNA and Cancer k|sk Score |n Iukush|ma Workers.
38
0
23
0
Four workers took once i.v. Vitamin C (25g) therapy at before they went, and
continuously took anti-oxidative supplements during the working period. No
significant change was seen in both Free DNA and Cancer Risk Score.
36
ng/mL
F
Cancer Risk Score
38
0
23
0
Before After work Before After work
Lect of ann-ox|danve nutr|non on Iree DNA and
Cancer k|sk Score before and aher S-6 weeks of
nuc|ear p|ant work at Iukush|ma.
Conc|us|on
37
(1) We evaluated the effects of radiation exposure on cancer
related gene expression in 17 men who worked 5-6 weeks at
the radiation contaminated area of Fukushima nuclear plant.
(2) After working, plasma free DNA was increased in 2 of 13
workers, and cancer risk score was increased in 3. After the
2 months intervention with intravenous Vitamin C and oral
anti-oxidative nutritional supplements, free DNA returned to
normal level and cancer risk score was significantly
decreased (p<0.05).
(3) No significant change was seen in 4 workers who took once
i.v. Vitamin C at before working and continuous intake of
anti-oxidative supplements during the working period.
(4) Increase in cancer risk by radiation exposure can be
protected by anti-oxidative nutritional intervention.
Fukushima workers in the radiation contaminated area
should take anti-oxidative nutrition therapy immediately.
"Cn|y V|tam|n C and other ann-ox|danve
nutr|nous supp|ements can save the
peop|e's ||fe from rad|anon |n[ury."
- ICI1 work|ng group
54 yrs. female, stomach cancer
3 yrs after IVC (58 yrs)
BW= 40 kg
My roposa|
Ma|nta|n|ng opnma| ann-ox|danon reserve by
tak|ng V|tam|n C and other ann-ox|danve
nutr|non |s essenna| for cancer prevennon and
treatment, ant-ag|ng, rad|o-protecnon and
opnmum hea|th.
42
Thank you !
http://www. iv-therapy.jp

You might also like