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Processing Principles

Food Processing and Food Microbiology

High Temperature Low Temperature Reduce Moisture Alter Gaseous Atmosphere Alter pH Packaging

Preservatives Chemical and Biological Irradiation/UV Radiation Sanitation and Handling Combination

Harrison, FDST 4030/6030

Processing and Food Microbiology

High Temperatures
Variety of equipment to achieve this
Immersion cookers, steam injectors, smokehouses, heating tunnels, ovens, etc.

High Temperatures
Smokehouse

Alkar.com

Harrison, FDST 4030/6030

Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

High Temperature
Spiral Cooker Use for fully cooked products Fully cooked items followed by chilling
Harrison, FDST 4030/6030 Processing and Food Microbiology

High Temperature
Immersion cooker

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Processing and Food Microbiology

Processing and Food Microbiology Harrison FDST 4030/6030

High Temperature

High Temperature

Harrison, FDST 4030/6030

Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

Frozen and Refrigerated Foods


Fruits Vegetables Meat Poultry Seafood Etc.

Frozen and Refrigerated Foods


Time to chill to desired temperature Cooling solids vs. liquids Means use to chill
Air chill (including CO2, liquid nitrogen) Liquid chill

Harrison, FDST 4030/6030

Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

Refrigeration

Refrigeration
Hydrochiller

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Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

Processing and Food Microbiology Harrison FDST 4030/6030

Refrigeration
Hydrocoolers

Blanched Foods
Blancher

Used to inactivate naturallyoccurring enzymes Commonly used for vegetables prior to freezing

Harrison, FDST 4030/6030

Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

Frozen Foods
Contact belt freezer

Frozen Foods
Tunnel freezer

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Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

Frozen Foods
Package spiral freezer

Frozen Foods
Spoilage and pathogenic microorganisms may be present
During freezing and frozen storage
Some may be injured Some may be killed
Not an effective, reliable means to reduce pathogen populations Exception might be parasites

While frozen concern would be whether or not they survive and become problems after thawing

Harrison, FDST 4030/6030

Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

Processing and Food Microbiology Harrison FDST 4030/6030

Frozen Foods
Processing
Blanching
Microbial numbers reduced some Typically only common with vegetables

Frozen Foods
Microbial spoilage problems- rare Pathogen problems
Rare Many frozen foods are cooked prior to consumption

Freezing methods

Control of problems
Sanitation Proper blanching and freezing

Harrison, FDST 4030/6030

Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

Dried Foods
Primary preservation principle in these foods may be the reduced water activity
However, typically couple with another hurdle - low pH. low temperature, reduced O2, chemical antimicrobials, etc.

Dried Foods
In the process:
May apply heat May include chemical antimicrobials May allow for pH reduction via fermentation or acidification May package product May store product at low temperature

If done properly
Typically no spoilage problems Typically no pathogen problems

Fungal spoilage can occur if aw is too high


Harrison, FDST 4030/6030 Processing and Food Microbiology Harrison, FDST 4030/6030 Processing and Food Microbiology

Dried Foods
Possible problems
Insufficient heat applied to get any heatrelated lethality Wrong concentration or combination of chemical antimicrobials used, gradients occur Desired pH is not attained and maintained Desired packaging material and gas mixture not used Stored at improper temperature Overall sanitation and handling practices
Harrison, FDST 4030/6030 Processing and Food Microbiology

Beef Jerky Processing


Cut and Trim Meat
May be acidic; May contain salt, etc. 140-160oC for example Wrap or vacuum pack Room temperature; Refrigerator

Marinate

Dry

Package

Store

Harrison, FDST 4030/6030

Processing and Food Microbiology

Processing and Food Microbiology Harrison FDST 4030/6030

Dried Fruit Processing


pH naturally low To prevent discoloration 140-160oC for example Wrap or vacuum pack Room temperature; Refrigerator

E. coli O157:H7, L. monocytogenes and Salmonella on jerky strips treated at 60C

Cut and Trim Fruit


Marinated Non-marinated After Drying <0.60 Pre-tmt After Drying 2.12

Antioxidant Dip
E. coli O157:H7 L. monocytogenes Salmonella

Pre-tmt

After Marin.

Dry

6.38

5.88

6.37

Package

4.50

2.71

<0.60

4.51

1.13

Store

5.84

5.33

1.16

6.19

2.56

Harrison, FDST 4030/6030

Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

Other Food Processing Methods


Radiation Packaging Aseptic packaging Antimicrobials Types and Uses UV light High pressure processing Pulsed electric fields Combinations to achieve Hurdles
Processing and Food Microbiology

Radiation
Ionizing radiation Electron beam sterilization
No radioactive waste is produced cold pasteurized or electronic pasteurized Electron beams are produced with energy ranges between 5-10 electron volts
Lethal effect due to disruption of DNA

Harrison, FDST 4030/6030

Harrison, FDST 4030/6030

Processing and Food Microbiology

Packaging
Plastic Antimicrobials incorporated into package Moisture controllers Oxygen scavengers Retort pouches Contoured cans Aseptic packages

Antimicrobials Types and Uses


Types and combinations Mode of application
Direct application Indirect application With packaging Sprayed Dipped

Harrison, FDST 4030/6030

Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

Processing and Food Microbiology Harrison FDST 4030/6030

UV light
UV at a wavelength of 253.7 nm is effective There is a time-exposure relationship Uses
Juice processors e.g., apple juice/cider Surface treatment for packaged foods e.g., packaged meats

UV light
Advantages:
No chemicals used Effective on a range of microorganisms Rapid, economical, simple operation

Disadvantages:
Low penetration power Shadowing

Harrison, FDST 4030/6030

Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

High pressure processing


Exposed to pressures of 200-1,000 Mpa Nonthermal Pressure applied uniformly to food Lethal effect by interruption of cellular functions Applications high value, sensitive products
e.g., guacamole

Pulsed electric fields


Application of short pulses of high electric fields
Food placed between 2 electrodes Pulses applied for microseconds Nonthermal Lethal effect - probably due to disruption of cell membrane and electroporation

Batch and continuous processing


Harrison, FDST 4030/6030 Processing and Food Microbiology Harrison, FDST 4030/6030 Processing and Food Microbiology

Canned Foods
Preserved by heat in hermetically sealed containers Cans or glass or flexible packages Commercial Sterility of Thermally Processed Foods
Defined in the Code of Federal Regulations Title 21, Part 113.3(e)

Commercial Sterility Testing of Canned Foods


Test at least 1 can/retort run Incubate can Visually inspect container and contents, measure container vacuum, check odor and pH If necessary: Microscopic examination, then subculture Duplicate tubes/plates inoculated to rule out lab contamination
Harrison, FDST 4030/6030 Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

Processing and Food Microbiology Harrison FDST 4030/6030

Low Acid Canned Foods


Vegetables (most), meat, poultry, seafood pH > 4.6 Commercially sterile products Primary concern - Clostridium botulinum
Concern - home canned vs. commercially canned

Appearance of Unopened Spoiled Cans


Flat - can ends normal or slightly concave Flipper - one end made convex by striking or heating can Springer - one end pushed in and the other pops out Soft swell - bulged ends can be dented by pushing one in Hard swell - both ends bulged and cannot push in
Harrison, FDST 4030/6030 Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

Spoilage in Canned Foods


Non-microbial spoilage
Incipient spoilage Hydrogen swells Non-enzymatic browning (Maillard reaction) Formulation errors and mishandling (e.g., freezing of cans) Enzymatic changes
Liquefaction, off-flavors, curdling, discoloration In some UHT and HTST products
Harrison, FDST 4030/6030 Processing and Food Microbiology

Microbiological Spoilage of Low Acid Canned Foods


Insufficient processing
Pure cultures of heat resistant sporeformers Could include C. botulinum

Post-processing problems - Container leakage Thermophilic spoilage due to inadequate cooling after processing or improper storage/distribution temperatures
Flat Sour Spoilage Thermophilic Anaerobe (TA) Sulfide Stinker
Harrison, FDST 4030/6030 Processing and Food Microbiology

Thermophilic spoilage of Low Acid Canned Foods


Flat Sour Spoilage

Microbiological Spoilage of Acid Canned Foods


pH < 4.6 Fruits, Acidified foods Spoilage
Combined effect of proper time/temperature treatment and pH yields stable product

Container not swollen; Product pH is lowered Sporeformers - e.g., Geobacillus stearothermophilus (formerly Bacillus stearothermophilus)

Thermophilic Anaerobe (TA)


Container swells - may burst; Product may have cheesy odor Thermoanaerobacterium thermosaccharolyticum (formerly Clostridium thermosaccharolyticum)

Pathogens
Almost none

Sulfide Stinker
Flat can; Contents are darkened; Contents have the odor of rotten eggs Clostridium nigrificans (formerly Desulfotomaculum nigrificans)
Harrison, FDST 4030/6030 Processing and Food Microbiology Harrison, FDST 4030/6030

Processing and Food Microbiology

Processing and Food Microbiology Harrison FDST 4030/6030

Microbiological Spoilage of Acid or Acidified Canned Foods


Insufficient processing
Butyric acid anaerobes - Butyric acid, CO2, H2 produced e.g., Clostridium pasteurianum Aciduric flat sours e.g., Bacillus coagulans in tomato products e.g., Alicyclobacillus spp. in fruit juices Heat resistant molds e.g., Byssochlamys fulva, Neosartorya fischeri, Talaromyces flavus Moldy taste and odor Fruit juices and concentrates Variety yeasts and asporogenous bacteria
Harrison, FDST 4030/6030 Processing and Food Microbiology

Microbiological Spoilage of Acid or Acidified Canned Foods


Thermophilic spoilage
Particularly in tomato products

Container leakage
Mixed and varied microflora possible Container may be flat of swollen

Harrison, FDST 4030/6030

Processing and Food Microbiology

Hurdles

Minimally Processed Foods


Fresh and fresh-cut produce for example

Combinations to achieve Hurdles Often used with minimally processed foods

Harrison, FDST 4030/6030

Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

Ready-to-eat-Foods
Variety of foods included Problem is the consumer typically would not have to heat or otherwise treat the food prior to consumption
No microbial inactivation step on the part of the consumer

Commodities - Outline
Microbiological characteristics of major food groups
Some characteristics of the food Natural microflora Spoilage issues Pathogen issues

Processing techniques commonly used

Harrison, FDST 4030/6030

Processing and Food Microbiology

Harrison, FDST 4030/6030

Processing and Food Microbiology

Processing and Food Microbiology Harrison FDST 4030/6030

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