Large Area Imaging from Carl Zeiss

ATLAS TM
Large Area Imaging for Unrivaled Productivity

The task of imaging tissue samples in histopathology is still widely based on the use of transmission electron microscopes (TEM). However, while the theoretically achievable resolution of these is usually not required in order to resolve the relevant structures in embedded and heavy metal stained material, its use certainly also limits the potential for process automation and hence productivity enhancement. With ATLASTM, Carl Zeiss is presenting a novel approach to address the challenge of acquiring a large number of tissue images for routine use in a highly automated and thus cost-effective fashion. Given suitable samples, unattended operation can be performed even over a period of days. Based on Carl Zeiss scanning electron microscopy technology, ATLASTM allows the acquisition of images of up to 32 k x 32 k pixels at nanometer resolution. This corresponds to a field of view of several tens of microns in an individual image or 256 images acquired on a TEM 2 k x 2 k camera at the same resolution. With the in-built tiling mechanism a large number of such images can be automatically acquired to cover an even larger sample area. Consequently ATLASTM provides the possibility to transfer the time-consuming process of identifying regions of interest and analyzing their high resolution information from the microscope to an off-line PC. During this time the scanning electron microscope can continue to acquire data effectively, therefore increasing the productivity of your electron microscopy lab.

We make it visible.

Large Area Imaging from Carl Zeiss

Key Benefits Full flexibility in choice of detectors for imaging including the STEM and BSE detectors for TEM-like images Highly automated, multi-site image acquisition process with automated stage motion, focus, stigmation, brightness and contrast adjustment as required In-built image tiling mechanism for acquiring multiple images at multiple sites to cover large sample areas at highest resolution Freely configurable individual image size from 1 k x 1 k to 32 k x 32 k pixels Viewer software tailored to efficiently handle multi-gigabyte images and image mosaics generated by ATLASTM including semi-automated stitching routines

Maximum Information Maximum Insight More than 160 years of experience in optics has laid the foundation for pioneering electron and ion beam microscopes from Carl Zeiss. Superior integration of imaging and analytical capabilities provides information beyond resolution, unlocking the best kept secrets of your sample. With a broad technology portfolio Carl Zeiss provides instruments both tailored to your requirements and adaptable to your evolving needs. With our highly versatile application solutions we endeavor to be your partner of choice. Superbly equipped, regional demo centers provide you with access to our applications expertise developed in collaboration with world-class partners in industry and academia. Global customer support is provided by the Carl Zeiss Group together with an extensive network of authorized dealers. Our mission at all times: Maximum Information Maximum Insight.

STEM-in-SEM images of rat hippocampus acquired on a ZEISS SUPRA 40 equipped with ATLASTM. After an electro-physiological experiment, the rat was glutaraldehyde perfused and the hippocampus was sectioned with vibrating knife to 70 m thick. The section was osmicated with potassium ferrocyanide en bloc, dehydrated in ethanol, embedded in Ladd LX112 epoxy via propylene oxide, ultrasectioned at 45 nm thick, post-section stained with uranyl acetate and lead citrate. (A) Low magnification overview image of a single mosaic site consisting of 6 x 2 tiles, each 49 m x 49 m, acquired using the GEMINI STEM detection system in a SUPRA FE-SEM. Each image tile was acquired at 2 nm per pixel. (B) A single 49 m STEM-in-SEM image, acquired at 2 nm per pixel. The inset square illustrates the comparative size of a TEM image acquired on a 4 k x 4 k camera, also at 2 nm per pixel. (C) Detail from an approximately 9 m x 9 m region of Image B. Note the image quality is comparable to results obtained by conventional TEM. At full resolution, key organelles are readily resolved including (D) post synaptic densities at synapses, (E) polyribosomes and (F) cross-sectioned microtubules suitable for serial tracing and dense reconstruction. Results courtesy of John Mendenhall, Center for Learning and Memory, University of Texas at Austin.

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Due to a policy of continuous development, we reserve the right to change specifications without notice. Version 11-11 Z-ATLASF by Carl Zeiss NTS, Oberkochen