2. A housewife made fruit pickles using unripe mango.

During the preparation, she placed the mango slice in water and later placed them in sugar solution. When the mango slice were in water, it was found that, the slice become turgid and their sizes increased. But when they were placed in the sugar solution, the slices become soft and shrunken. Based on the above situation, plan a laboratory experiment to determine the concentration of sucrose which is isotonic to the cell sap of the mango. The planning of the experiment should cover the following aspects : Problem statement Aim of investigation Hypothesis Variables List of apparatus and materials Technique used Experimental procedure or method Presentation of data Conclusion (17 marks)

Question 2 (Scheme) Score 3 1. Problem statement Explanation Able to state the problem statement correctly. Sample answer : 1. How does the difference concentration of sucrose affect the mass/length of mango tissue? 2. What is the concentration of sucrose solution which is isotonic to the cell sap of mango tissue? Able to state the problem statement of identified problem but incorrect Sample answer : 1. What is the effect of concentration of sucrose solution? 2. What is the mass/length of mango tissue? Able to state the idea only Sample answer : Sucrose solution affect the mass/length of mango tissue? Remarks Tick MV-concentration of sucrose solution RV-final mass/length MV @ RV only

No response or wrong response

2. Aim of study Sample answer Able to state the objective of investigate correctly. 1. To study the concentration of sucrose solution which is isotonic to the cell sap of mango tissue. 2. To study effect of difference concentration of sucrose to the mass/length of mango tissue

Remarks Must use any solution/plant cell

3. Making Hypothesis Score Explanation 3 Able to state the hypothesis correctly by relating two variables correctly Sample answer : 1. When concentration of sucrose solution which is isotonic to the cell sap of mango tissue, there is no gain in size/mass/length 2. The higher the concentration of sucrose solution, the less the size/mass/length of mango tissue Able to state the hypothesis slightly incorrect. Sample answer; 1. The isotonic solution has no effect on the plant cell sap on the mass/length of mango tissue. 2. The difference of concentration of sucrose solution influence the final mass/length of mango tissue. Able to state the idea only Sample answer: 1. The concentration of sucrose solution affect the plant cell sap No response or wrong response

Remarks Tick

Must have MV, RV and H

MV, RV MV, H RV, H

4. Variables Sample answer

Remarks

All three variables must be correct: - Manipulated variable : Concentration of sucrose solution that used - Responding variable : final mass/length of mango tissue - Constant variable : initial mass/length of mango tissue, volume of solution/time 5. Listing of materials and apparatus Score Explanation 3 Able to state all the materials and apparatus: Sample answer : - Materials : mango, distilled water and sucrose solution - Apparatus : test tube, knife, forceps, cork borer, filter paper, stop watch, ruler/electronic balance. 2 1 0 Able to state 3 of the materials and 4 apparatus Able to state 2 of the materials and 2 apparatus No response or wrong response

Tick

Remarks Tick

6. Technique Sample answer Measuring the mass/length of mango/potato strips with electronic balance/ruler

Remark Bonus 1 Tick

7. Method/ procedure of investigation Score 3 Explanation Able to list down the complete procedures/steps used based on the following five criteria P1, P2, P3, P4 and P5 K1 : Procedures of assembling the apparatus and materials (step 1-5) Remarks Tick

K2 : Method to operating manipulated variables (step 3) K3 : Method to determine responding variable (step 6) K4 : Method of controlling variable (step 3.4.5) K5 : Method to take precaution steps (step 6) Sample answer: 1. 7 test tube are labeled as A, B, C, D, E, F and G 2. Test tube A is filled with 25ml distilled water. 3. Each test tube is filled with 25ml of difference solution as shown below: Test tube B:0.1M sucrose solution Test tube C:0.1M sucrose solution Test tube D:0.1M sucrose solution Test tube E:0.1M sucrose solution Test tube F:0.1M sucrose solution Test tube G:0.1M sucrose solution 4. A cork borer is used to bore 7 mango strips. Each strip is cut to a length of 5cm/ measure the mass as 10g. 5. 1 strip are placed in each test tube and left for an hour. 6. After an hour, the strips are removed. The mango strips are wiped dry and the mass/length of each strip is measured. 7. The measurements are recorded. 8. A graph of the change in mass/length of the mango against the concentration of sucrose solution is plotted.

2 1 0

Able to state 3-4 criteria Able to state at least 2 creteria No response or wrong response

8. Data presentation // presentation of result Sample answer Remarks

Able to construct a table based on these criteria 1) Concentration of sucrose solution 2) the initial and final mass/length of mango strips 3)the change in the mass/length of mango strips
Concentration of sucrose solution 0(distilled water) 0.1 M 0.2M 0.3M 0.4M 0.5M 0.6M Initial mass/length(g/cm) 1 2 3 Average 1 final mass/length(g/cm) 2 3 Average

Bonus 2 tick (without data)

Predicted graph Graph of change in mass/length of mango strip against concentration of sucrose solution
change in mass/length (g/cm)

0
0.1 of sucrose solution,M 0.2 0.3 0.4 0.5 0.6 0.7Concentration

9. Conclusion Sample answer 1. When concentration of sucrose solution which is isotonic to the cell sap of mango tissue, there is no gain in size/mass/length 2. The higher the concentration of sucrose solution, the less the size/mass/length of mango tissue Remarks Bonus 1 Tick

Aspect 3 : Planning 8-9 Tick = 3 M 4- 7 Tick = 2 M 1-3 Tick = 1 M

Summary 1. Problem statement 2. Hypothesis 3. Planning 4. Procedures 5. Apparatus and materials 6. Bonus Totals = 17 marks = 3 marks = 3 marks = 3 marks = 3 marks = 3 marks = 2 marks