IIANEHil:HIMION 0EI:I:AAIAI:

TMHMA BIOXHMEIAI: & BIOTEXNOAOrJAE

HAOYT!!NOE 26
41221 AAPIEA
THAIFAX. (2410) 565277/90
Email: dkouret @uth.gr


UNIVERSITY OF THESSAL Y
BIOCHEMISTRY & BIOTECHNOLOGY DEPT
PLOUTONOS 26
LARISSA, 41221-GREECE
TEL/FAX. (302410) 565277/90
Email: dkouret@uth.gr
Demetrios Kouretas
Professor
CERTIFICATE OF ANALYSIS AND CLASSIFICATION OF
AGRINIO
COMPANY: ATMOS LAB
COMMERCIAL NAME OF THE PRODUCT: AGRINIO
TOTAL NUMBER: 01/AGRINIO 112
ANALYSIS
(Gas Chromatography -Mass Spectrometry and Liquid Chromatography-Mass Spectrometry)
RESULTS
table 1.
percentage
Limit of analysis
substance
(%w/v)
(LOQ) classification
( m)
propylene glycol 3,21
0,198
no
glycerol 83,59
1,187
no
water II ,6997
Acute toxicity 1
nicotine 1,5 0,1
Acute toxicity 3
Chronic toxicity 2 in
aquatic organisms
total 99,9997
Classification of the liquid Agrinio
According to Greek regulation ( FEK 1214/B'/19.9.2002), classification of particular ingredients
of the mixture and according to EU Regulation 1272/2008 this product with 99.9991%
composition, is classified as non hazardous for human health, environment and according to its
physicochemical properties.
NYTROZAMINE ANALYSIS
(Liquid Chromatography -Mass Spectrometry)
table 2.
name
4-(Methylnitrosamino )-1-(3-
pyridyl)-1-butanol
4'-(nitrosomethylamino )-1-(3-
pyridyl)-1-butanone
N-nitrosoanatabine
N-nitrosoanabasine
RESULTS
concentration
(ppm)
ND*
ND
ND
ND
(LOQ)
(ppm)
0,01
0,01
0,05
0,01
POLYCYCLIC AROMATIC HYDROCARBONS (PAHs) ANALYSIS
(Gas Chromatography -Mass Spectrometry)
RESULTS
table 3.
name Concentration
(LOQ)
(ppm)
(ppm)
acenaphtylene ND* 0,005
fluorene ND 0,010
phenanthrene ND 0,005
anthracene ND 0,006
pyrene ND 0,003
benzo(a)anthracene ND 0,003
chrysene ND 0,003
benzo(k)fluoranthene ND 0,060
benzo(b )fluoranthene ND 0,006
benzo(g,h,i)perylene ND 0,014
dibenzo(a,h)anthracene ND 0,021
indeno( 1 ,2,3-CD)pyrene ND 0,022
Demetrios Kouretas, PhD
Professor
Biochemical Toxicology
DANEnn:ntMION 0EI:I:AAIAI:
TMHMA BIOXHMEIAI: & BIOTEXNOAOrlAI:
DAOYTflNOI: 26
41221 AAPII:A
THA/FAX. (2410) 565277/90
Email: dkouret @uth.gr


UNIVERSITY OF THESSAL Y
BIOCHEMISTRY & BIOTECHNOLOGY DEPT
PLOUTONOS 26
LARISSA, 41221-GREECE
TEL/FAX. (302410) 565277/90
Email: dkouret@uth.gr
Demetrios Kouretas
Professor
CERIFICATE OF ANALYSIS AND CLASSIFICATIONS
OF APACHE
COMPANY: ATMOS LAB
COMMERCIAL NAME OF THE PRODUCT: APACHE
TOTAL NUMBER: 011 APACHE 112
ANALYSIS
(Gas Chromatography -Mass Spectrometry and Liquid Chromatography-Mass Spectrometry)
RESULTS
table 1.
substance
persentage
Limit of
analysis (LOQ)
( m)
propylene glycol
glycerol
water
nicotine
total
Classification of the liquid Apache
(%w/v)
4,05
84,93
9,7144
1,3
99,9944
0,198
I ,187
0,1
classification
no
no
Acute toxicity 1
Acute toxicity 3
Chronic toxicity 2 in
aquatic organisms
According to Greek regulation ( FEK 1214/B'/19.9.2002), classification of particular ingredients
of the mixture and according to EU Regulation 1272/2008 this product with 99.9991 %
composition, is classified as non hazardous for human health, environment and according to its
physicochemical properties.
NYTROZAMINE ANALYSIS
(Liquid Chromatography -Mass Spectrometry)
table 2.
name
4-(Methylnitrosamino )-1-(3-
pyridyl)-1-butanol
4'-(nitrosomethylarnino )-1-(3-
pyridyl)-1-butanone
N -nitrosoanatabine
N -nitrosoanabasine
RESULTS
concentration
(ppm)
ND*
ND
ND
ND
(LOQ)
(ppm)
0,01
0,01
0,05
0,01
POLYCYCLIC AROMATIC HYDROCARBONS (PAHs) ANALYSIS
(Gas Chromatography -Mass Spectrometry)
RESULTS
table 3.
name conentration
(LOQ)
(ppm)
(ppm)
acenaphtylene ND* 0,005
fluorene ND 0,010
phenanthrene ND 0,005
anthracene ND 0,006
pyrene ND 0,003
benzo(a)anthracene ND 0,003
chrysene ND 0,003
benzo(k)fluoranthene ND 0,060
benzo(b )fluoranthene ND 0,006
benzo(g,h,i)perylene ND 0,014
dibenzo(a,h)anthracene ND 0,021
indeno( 1 ,2,3-CD )pyrene ND 0,022
Demetrios Kouretas, PhD
Professor
Biochemical Toxicology
nANEnt:!:::TIIMION 0EI:I:AAIAI:
TMHMA BIOXHMEIAI: & BIOTEXNOAOriAI:
nAOYT!!NOI: 26
41221 AAPII:A
TIIAIFAX. (2410) 565277/90
Email: dkourct@uth.gr


UNIVERSITY OF THESSAL Y
BIOCHEMISTRY & BIOTECHNOLOGY DEPT
PLOUTONOS 26
LARISSA, 41221-GREECE
TEL/FAX. (302410) 565277/90
Email: dkourct @uth.gr
Demctrios Kouretas
Professor
CERTIFICATE OF ANALYSIS AND CLASSIFICATON
OF BARBARA
COMPANY: ATMOS LAB
COMMERCIAL NAME OF THE PRODUCT: BARBARA
TOTAL NUMBER: OJ/BARBARA /12
ANALYSIS
(Gas Chromatography -Mass Spectrometry and Liquid Chromatography-Mass Spectrometry)
table 1.
substance
propylene glycol
glycerol
water
nicotine
total
RESULTS
percentage
(%w/v)
3,67
80,19
15,0376
1,1
99,9976
Limit of analysis
(LOQ)
( m)
0,198
I ,187
0,1
Classification of the liquid Barbara
classification
no
no
Acute toxicity 1
Acute toxicity 3
Chronic toxicity 2 in
aquatic organisms
According to Greek regulation ( FEK 1214/B'/19.9.2002) , classification of particular ingredients
of the mixture and according to EU Regulation 1272/2008 this product with 99.9991%
composition, is classified as non hazardous for human health, environment and according to its
ph ysicochernical properties.
NYTROZAMINE ANALYSIS
(Liquid Chromatography -Mass Spectrometry)
RESULTS
table 2.
name concentration (LOQ)
(ppm) (ppm)
4-(Methylnitrosamino )-1-(3-
pyridyl)-1-butanol ND* 0,01
4'-(nitrosomethylamino )-1-(3-
pyridyl)-1-butanone ND 0,01
N-ni trosoanatabine ND 0,05
N -nitrosoanabasine ND 0,01
POLYCYCLIC AROMATIC HYDROCARBONS (PAHs) ANALYSIS
(Gas Chromatography -Mass Spectrometry)
RESULTS
Table 3.
name concentration
(LOQ)
(ppm)
(ppm)
acenaphtylene ND* 0,005
fluorene ND 0,010
phenanthrene ND 0,005
anthracene ND 0,006
pyrene ND 0,003
benzo(a)anthracene ND 0,003
chrysene ND 0,003
benzo(k)fluoranthene ND 0,060
benzo(b )fluoranthene ND 0,006
benzo(g,h,i)perylene ND 0,014
dibenzo( a,h )anthracene ND 0,021
indeno( 1 ,2,3-CD)pyrene ND 0,022
Demetrios Kouretas, PhD
Professor
Biochemical Toxicology
fiANEOJI:TIIMION 0EI:I:AAIAI:
TMHMA BIOXHMEIAI: & BIOTEXNOAOriAI:
fiAOYT!!NOI: 26
41221 AAPII:A
THA/FAX. (2410) 565277/90
Email: dkouret@uth.gr


UNIVERSITY OF THESSAL Y
BIOCHEMISTRY & BIOTECHNOLOGY DEPT
PLOUTONOS 26
LARISSA, 41221-GREECE
TEL/FAX. (302410) 565277/90
Email: dkouret@uth.gr
Demetrios Kouretas
Professor
CERTIFICATE OF ANALYSIS AND CLASSIFICATION
OFBEBECA
COMPANY: ATMOS LAB
COMMERCIAL NAME OF PRODUCT: BEBECA
TOTAL NUMBER: 01/BEBECA/12
ANALYSIS
(Gas Chromatography -Mass Spectrometry and Liquid Chromatography-Mass Spectrometry)
table 1.
substance
methyl cyclopentenolone
ethyl maltol
ethyl vanillin
propylene glycol
glycerol
water
nicotine
total
RESULTS
Percentage
(%w/v)
0,503
1,130
0,004
9,35
85,86
1,6522
1,5
99,9992
Limit of analysis
(LOQ)
( m)
0,121
0, 135
0,032
0,198
1,187
0,1
classification
no
Oral acute toxicity group 4
Skin irritation group 2
Eye irritation group 2
Special toxicity in target
organs group 3
no
no
Acute toxicity 1
Acute toxicity 3
Chronic toxicity 2 in
aquatic organisms
Classification of the liquid Bebeca
According to Greek regulation ( FEK 1214/B'/19.9.2002), classification of particular ingredients
of the mixture and according to EU Regulation 1272/2008 this product with 99.9991%
composition, is classified as non hazardous for human health, environment and according to its
physicochemical properties
NYTROZAMINE ANALYSIS
(Liquid Chromatography -Mass Spectrometry)
table 2.
name
4-(Methylnitrosamino )-1-(3-
pyridyl)-1-butanol
4'-(nitrosomethylamino )-1-(3-
pyridyl)-1-butanone
N -nitrosoanatabine
N -nitrosoanabasine
RESULTS
concentration
(ppm)
ND*
ND
ND
ND
(LOQ)
(ppm)
0,01
0,01
0,05
0,01
POLYCYCLIC AROMATIC HYDROCARBONS (PAHs) ANALYSIS
(Gas Chromatography -Mass Spectrometry)
RESULTS
table 3.
name concentration
(LOQ)
(ppm)
(ppm)
acenaphtylene ND* 0,005
fluorene ND 0,010
phenanthrene ND 0,005
anthracene ND 0,006
pyrene ND 0,003
benzo(a)anthracene ND 0,003
chrysene ND 0,003
benzo(k)fluoranthene ND 0,060
benzo(b )fluoranthene ND 0,006
benzo(g,h,i )perylene ND 0,014
dibenzo(a,h)anthracene ND 0,021
indeno( 1 ,2,3-CD )pyrene ND 0,022
Demetrios Kouretas, PhD
Professor
Biochemical Toxicology
nANEniETHMION 0EEEAAIAE
TMHMA BIOXHMEIAE & BIOTEXNOAOrtAE
nAOYT!!NOE 26
41221 AAPIEA
THAIFAX. (2410) 565277/90
Email: dkouret@uth.gr


UNIVERSITY OF THESSAL Y
BIOCHEMISTRY & BIOTECHNOLOGY DEPT
PLOUTONOS 26
LARISSA, 41221-GREECE
TEL/FAX. (302410) 565277/90
Email: dkouret@uth.gr
Demetrios Kouretas
Professor
CERTIFICATE OF ANALYSIS AND CLASSIFICATION
OFCAMILA
COMPANY: ATMOS LAB
COMMERCIAL NAME OF PRODUCT: CAMll..A
TOTAL NUMBER: 011 CAMILA 112
ANALYSIS
(Gas Chromatography -Mass Spectrometry and Liquid Chromatography-Mass Spectrometry)
RESULTS
Table 1.
Percentage
Limit of analysis
substance
(%w/v)
(LOQ) classification
( m)
Oral acute toxicity group 4
Skin irritation group 2
ethyl vanillin 0,001 0,032
Eye irritation group 2
Special toxicity in target
organ group 3
propylene glycol 15,52
0,198
no
80,70
1,187
no
water 2,2773
Acute toxicity 1
nicotine 1,5 0,1
Acute toxicity 3
Chronic toxicity 2 in
aguatic organisms
total 99,9983
Classification of the liquid CAMILA
According to Greek regulation ( FEK 1214/B'/19.9.2002), classification of particular ingredients
of the mixture and according to EU Regulation 1272/2008 this product with 99.9991%
composition, is classified as non hazardous for human health, environment and according to its
physicochemical properties.
NYTROZAMINE ANALYSIS
(Liquid Chromatography -Mass Spectrometry)
table 2.
name
4-(Methylnitrosamino )-1-(3-
pyridyl)-1-butanol
4'-(nitrosomethylarnino )-1-(3-
pyridyl)-1-butanone
N -nitrosoanatabine
N -nitrosoanabasine
RESULTS
concentration
(ppm)
ND*
ND
ND
ND
(LOQ)
(ppm)
0,01
0,01
0,05
0,01
POLYCYCLIC AROMATIC HYDROCARBONS (PAHs) ANALYSIS
(Gas Chromatography -Mass Spectrometry)
RESULTS
total 3.
name concentration
(LOQ)
(ppm)
(ppm)
acenaphtylene ND* 0,005
fluorene ND 0,010
phenanthrene ND 0,005
anthracene ND 0,006
pyrene ND 0,003
benzo(a)anthracene ND 0,003
chrysene ND 0,003
benzo(k)fluoranthene ND 0,060
benzo(b )fluoranthene ND 0,006
benzo(g,h,i )pery lene ND 0,014
dibenzo(a,h)anthracene ND 0,021
indeno( 1 ,2,3-CD )pyrene ND 0,022
Demetrios Kouretas, PhD
Professor
Biochemical Toxicology
flANEllll:THMION 0EI:I:AAIAI:
TMHMA BIOXHMEIAI: & BIOTEXNOAOriAI:
nAOYT!!NOI: 26
41221 AAPII:A
THAIFAX. (2410) 565277/90
Email: dkouret@uth.gr


UNIVERSITY OF THESSAL Y
BIOCHEMISTRY & BIOTECHNOLOGY DEPT
PLOUTONOS 26
LARISSA, 41221-GREECE
TEL/FAX. (302410) 565277/90
Email: dkouret@uth.gr
Demetrios Kouretas
Professor
CERTIFICATE OF ANALYSIS AND CLASSIFICATION
OFKAPNOS
COMPANY: ATMOS LAB
COMMERCIAL NAME OF THE PRODUCT: KAPNOS
TOTAL NUMBER: 0 1/KAPNOS/ 12
ANALYSIS
(Gas Chromatography -Mass Spectrometry and Liquid Chromatography-Mass Spectrometry)
table 1.
substance
propylene glycol
glycerol
water
nicotine
total
RESULTS
percentage
(%w/v)
3,28
89,61
5,6996
1,4
99,9896
Limit of analysis
(LOQ)
( m)
0,198
1, 187
0,1
Classification of the liquid KAPNOS
classification
no
no
Acute toxicity 1
Acute toxicity 3
Chronic toxicity 2 in
aquatic organisms
According to Greek regulation ( FEK 1214/B '119 .9 .2002) , classification of particular ingredients
of the mixture and according to EU Regulation 1272/2008 this product with 99.9991 %
composition, is classified as non hazardous for human health, environment and according to its
physicochemical properties.
NYTROZAMINE ANALYSIS
(Liquid Chromatography -Mass Spectrometry)
RESULTS
table 2.
name concentration
(LOQ)
(ppm)
(ppm)
4-(Methylnitrosamino )-1-(3-
pyridyl)-1-butanol ND* 0,01
4'-(nitrosomethylamino )-1-(3-
pyridyl)-1-butanone ND 0,01
N -nitrosoanatabine ND 0,05
N -nitrosoanabasine ND 0,01
POLYCYCLIC AROMATIC HYDROCARBONS (PAHs) ANALYSIS
(Gas Chromatography -Mass Spectrometry)
RESULTS
table 3.
name concentration
(LOQ)
(ppm)
(ppm)
acenaphtylene ND* 0,005
fluorene ND 0,010
phenanthrene ND 0,005
anthracene ND 0,006
pyrene ND 0,003
benzo(a)anthracene ND 0,003
chrysene ND 0,003
benzo(k)fluoranthene ND 0,060
benzo(b )fluoranthene ND 0,006
benzo(g,h,i )perylene ND 0,014
dibenzo(a,h)anthracene ND 0,021
indeno( 1 ,2,3-CD)pyrene ND 0,022
Demetrios Kouretas, PhD
Professor
Biochemical Toxicology