Procedure: Introduction:

ANALYSIS OF MOUTHWASH

Part I: Commercial mouthwashes are mixtures of water, alcohol, dyes, flavorings, and other compounds. Prepare standard solutions of alcohol content of thethe following listbe l. This experiment will allow the ethanol according to mouthwash to using determined. If fifty ethanol. Be careful to make accurate measurements! Pipette the the 95% milliliters each of alcohol and water are mixed, the total volume of mixture will not equal and add to a 100mL volumetric flask. Then, carefully fill to the alcohol, one hundred milliliters. This reduction in volume is due the flask to attractive forces between the alcohol and water. Since the volumes of alcohol and water the 100mL line with distilled water. are not additive when mixed, a calibration curve must first be prepared. It will provide the correction needed for alcohol-water mixtures. The calibration curve will be prepared by % Volume Alcohol 95% alcohol (mL/100mL) placing a known amount of alcohol in an alcohol-water mixture. The observed percentage 5% 5.26mL of alcohol will be plotted against the true percentage of alcohol. 10% 10.53mL 20% 21.05mL 30% 31.58mL Once the calibration data is collected and plotted, samples of mouthwashes will be analyzed using the gas chromatograph. The observed percentage of alcohol will be determined and the following conditions from the edit menu calibration graph. These true percentage will be found using the by selecting 2. Set values may then be compared with the values reported by the manufacturer. channels then selecting channel 1 and temperature change. Start 140 C Hold 10 minutes Ramp 0 degrees/min Until the Purpose: temperature is 140 C 3. Obtain a experiment is to determine the amount of alcohol with the sample The purpose of this microliter syringe. Rinse the syringe several timesin a brand of to mouthwash. be tested. Then obtain 2 microliters of the sample in the syringe. 4. Insert the needle into the GC. Press the plunger, and start the run and Equipment/Materials: the same time. 5. After a peak is recorded, press the end button on the computer. ethanol distilled water 6. 100To obtain the area under the curve, or buret edit drop down menu and ml, volumetric flasks pipet select the option. Select gaschoose the Manual integration10 L syringe the rubber band icon found on chromatograph the bottom left of the graphed results. Left click and drag across the bottom mouthwash samples . of the curve to create a baseline. This will allow the program to calculate a Safety: more accurate area. 7. Select goggles then Results to obtain area data. Record results in the An apron andView and should be worn in the laboratory at all times. data table below. Complete steps this experiment should pose no significant safety 20%, The chemicals used in 3 through 5 for the following standards: 5%, 10%, hazards. and 30% alcohol. Good laboratory procedure should be followed at all times. Percent Alcohol v/v 5% 10% 20% 30% Area Due to Alcohol

9. Prepare a graph with the area on the x - axis, and the percentage of alcohol on the y - axis. Draw a "best fit" line connecting the points on your graph.

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Science theMotion 2. What was the AIR GC of ANALYSISsyringeALCOHOLS before purpose SEPARATION OF several times rinsing in OF inserting your sample into the GC?TEACHER MOUTHWASH Materials List TEACHER NOTES NOTES Lab Time: 40-50 minutes 3. How did the accepted values match the percentage of alcohol in your Lab mouthwash samples? Explain your results. Part II:Time: 45 minutes Considerations: Lab: Air GC Separation samples. 1. Obtain mouthwash of Alcohols In order to reduce the time of this lab, the alcohol standards could be prepared ahead of time. The lab could also be done not using the standards at all and just determine Number of Lab Groups Prepared: Answersinprocedure 3 the % of 2. Followto Question:through 5 from Part I with your mouthwash samples. Be alcohol the mouthwash by the area under the peaks. sure to rinse the syringe several times with the mouthwash sample you are Hardware running before you insert the needle into the GC. Equipment perimportant to run known below: of possible components in Returned 1. WhyEnterit labresults onPacked samples 3. was your group the table Sample data is included below: Air GC, Area due to Alcohol LaptopBrand with Peak Simple the unknown mixture? Gas Chromatograph - Alcohol Calibration Curve Cords: serial cable, Extensions: (v/v) Percent power cord and Area Due retention computerAlcoholwas necessary to determine theto Alcohol times in order to This adaptor 30 0.306 This software activity Copy ofthe20 can be done with vanilla extract. identify components in the unknown mixture. 0.198 Printer (optional) 10 0.125 Safety Goggles 5 0.0607 2. If a supposedly pure sample was properly injected into the gas chromatograph Brand Area Due to % Alcohol Accepted Value Consumables Analysis of Alcohol and several peaks were observed, what can be concluded about the sample? Materials Packed Returned List. Cool Mint .255 24.7 21.6 10 microliter syringefor the mouthwash brands on 1. area Weis Red Find the contained some volatile impurities. your standards graph. With a .156 14.9 kimwipes It ruler, draw a straight line, parallel to the y - axis, until you intersect the Cepacol .148 14.1 14 Unknown samples: graph. Draw a line straight down, parallel to the x - axis, to standards line Rite Aid leastfind the percentage of alcohol sample in the mouthwash sample. Enter .076 6.97 7.5 At Rinse three Green Mint this value onto the data table below. 18.9 Known Samples: .217 3.Red is it important to clean the syringe between samples? Why Lavoris 2. Look at the back panel of your6.72 .0734 Methanol mouthwash samples. Find the alcohol Acetone content, and record the accepted value on the data table below. Commercial 1-proponal brands of mouthwash vary widely in their alcohol contents. The may percentage of alcohol may beprevious substance will30%. If possible,the gas less than 10% to over be Acceptedby select detected Value Brand 2-proponal Any trace of the% Alcohol brands so that this range may be observed. Others TBA chromatograph and produce an additional peak Lab Handouts 4. Why is it important to wipe the needle before injecting the sample into the instrument? Question If any sample is on the outside of the needle it will be vaporized and 1. Whythrough the columngraph usingsample is injected, thus producing travel did you prepare a before the standards with known alcohol contents? What Law did this demonstrate? double peaks.