UV-Visible Spectroscopy

LECTURE 4 ORGANIC CHEMISTRY 1 FS AY 2010-2011 West Kristian D Paraiso, RPh

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Electromagnetic radiation
Spectroscopy
Study of the interaction between matter and electromagnetic radiation

Electromagnetic radiation
Radiant energy having the properties of both a particle and a wave

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Electromagnetic radiation

UV Visible

180-400 nm 400-720 nm

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Electromagnetic radiation

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Electronic transitions

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Spectrophotometer

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UV Spectrum of acetone

max wavelength of maximum absorbance

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Chromophores and auxochromes

Chromophore part of the molecule which absorbs UV/visible light

Auxochrome substituent which alters max and absorption intensity when attached to a chromophore

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Red shift and blue shift

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Red shift and blue shift

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Beers Law

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Beers Law
A solution of 4-methyl-3-penten-2-one in ethanol shows an absorbance of 0.52 at 236 nm in a cell with a 1-cm light path. Its molar absorptivity in ethanol at that wavelength is 12,600. What is the concentration of the compound?

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Effect of conjugation on max

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Effect of conjugation on max

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Exercises
How could one use UV spectroscopy to distinguish between the compounds in each of the following pairs?

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Calculations of max
To the base number,
1. + 30 for each extra conjugated double bond 2. + 5 if a conjugated double bond is an exocyclic double bond 3. + 36 for conjugated double bonds that are frozen in the s-cis conformation 4. + 5 for each alkyl group or halogen bonded to the conjugated system of a diene 5. + 10 for an -substituent of a conjugated aldehyde / ketone 6. + 12 for a -substituent of a conjugated aldehyde / ketone
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Calculations of max
Compute for the max of the following:

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Visible spectrum and color

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Visible spectrum and color

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Visible spectrum and color

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