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FY2011 Obama administration proposes around $159.3 billion for the Iraq and Afghanistan wars (wikipedia)
Study Design
Cohort Overweight women (12-15, obese sedentary, 100 < glucose < 128 mg/dL )
14 week diet* and exercise intervention Weight management and exercise (4 days/week, 30-40 min)
*same diet for all subjects = same background metabolites due to diet!
Sean Adams
Questions:
1. how does X affect metabolite baselines and excursion profiles 2. what are the independent metabolic markers for X
* Analyses I will talk about
Data Properties:
Structure
15 samples x 3210 (48, 150) 321 metabolites x 10 (3210) 2 endpoints 5 time-points (10)
Univariate Statistics:
risk = 1-(1-p.value)tests
Multivariate Analyses
219021
glucose (GC/TOF)
219021
Loadings on the first latent variable (x-axis) can be used to interpret the multivariate changes in metabolites which are correlated with time during the OGTT
Determine in-sample (Q2) and outof-sample error (RMSEP) and compare to a random model permutation tests training/testing
Model Training/Testing
Data set selection/splitting is not trivial
Where should these guys go?
Use networks to interpret statistical and multivariate results within a biochemical and mathematical context
Basic anetwork ingredients Make adjacency matrix (what is connected)
product/precursor (KEGG rpairs) chemical similarity (Tanimoto distances) Dependency (partial correlation)
pink () cyan ()
Future Goals
ExCytR: Excel + Cytoscape + R = Awesome GUI for generating mapped networks Devium Dynamic Multivariate Data Analysis and Visualization Platform GUI for multivariate analysis of Omics data Successor to imDEV Fork me on GitHub: https://github.com/dgrapov/devium
The stack: