pH Meter Calibration

NOTE: ALWAYS RINSE THE PROBE WITH DI WATER IN BETWEEN EACH MEASUREMENT. Use the squirt bottle to rinse and the plastic beaker to collect the water. Two buffer calibration should be done before any measurements are taken. One buffer calibration should be done every two hours. 1. ALWAYS start with the yellow pH 7 buffer as the first calibration buffer. 2. Use fresh (< 1 week), room temperature buffers. They are stored in the refrigerator and should be taken out in time to reach room temperature. The pH meter does display temperature, which should be taken into account when using the buffers. 3. The buffers should be stirred using a stir plate and magnetic stirrer while they are being measured for calibration. 4. Use the two buffers that bracket the expected range of measurements. For example, if your samples are expected to be acidic, use the pH 7 and pH 4 buffers to calibrate. If they are expected to be basic use the pH 7 and pH 10 buffers. 5. Rinse the electrode and place into the first buffer. 6. Press the 2nd then the cal keys. CALIBRATION is displayed above the main field and the time and date of the last calibration are displayed. After a few seconds, P1 is displayed in lower field. P1 indicates that the meter is ready for the first buffer and a value has not yet been entered. When READY is displayed, indicating electrode stability, the reading begins to flash, press the yes key. The display will remain frozen for two seconds. Then P2 will be displayed in the lower field indicating that the meter is ready for the second buffer. 7. Rinse the electrode and place into the second buffer. When READY is displayed, press the yes key. Press the measure key to end calibration. SLP appears in the lower field with the actual electrode slope, in percent, in the field. 8. The meter automatically advances to the measure mode. MEASURE is displayed above the main field. 9. Rinse the electrode and place into sample. Record pH directly from the meter display and temperature from the lower field. ONE Buffer Calibration 1. Select one buffer that closely approximates the expected sample pH. 2. Repeat steps 5 and 6 from above. 3. Press the measure key. SLP will be displayed in the lower field and the electrode slope in memory in the main field. If necessary, enter the correct electrode slope determined by the two point calibration and press the yes key. If slope value is unknown, enter 100.0 or perform a two buffer calibration. 4. Rinse the electrodes and place into sample. Read the pH directly from the main display and temperature from the lower field.

pH Electrode
Storage When not in use, the pH electrode should always be stored in a storage solution of 1 gram of KCl per 200ml of either the Buffer 4 or 7. Cleaning 1. soak electrode in 0.1 M HCl ofr 15-20 minutes. 2. rinse with DI H2O 3. Recondition by allowing to soak in either a 4 or 7 buffer for 30 minutes