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[1] HIV nephropathy can result from direct kidney infection with HIV or from the adverse effects of antiretroviral drugs (see the image below).[2, 3] Further, patients with HIV disease are at risk for developing prerenal azotemia due to volume depletion resulting from salt wasting, poor nutrition, nausea, or vomiting.
Types of electrolyte abnormalities observed with some of the drugs used to treat opportunistic infections in patients with human immunodeficiency virus (HIV). ARF stands for acute renal failure. HIV-associated nephropathy (HIVAN), formerly known as AIDS-associated nephropathy, is characterized by the following findings:
Nephrotic range proteinuria Azotemia Normal to large kidneys on ultrasound images Normal pressure Focal segmental glomerulosclerosis (FSGS) on renal biopsy findings
Although FSGS is the predominant glomerular lesion in HIVAN, other reported glomerular lesions in patients with HIV include IgA nephropathy, cryoglobulinemia, amyloidosis, and a lupuslike immune complex glomerulopathy. In the preantiretroviral therapy era, HIVAN was characterized by rapid progression to renal failure and end-stage renal disease (ESRD) leading to the need for dialysis. Highly active antiretroviral therapy (HAART) has changed the natural course of this disease, increasing the importance of prompt diagnosis and proper care. Baseline estimated glomerular filtration should be obtained and renal function monitored during HAART.[4] Physicians must consider HIVAN in patients who develop proteinuria. For other discussions on management of HIV infection, see HIV Disease, Pediatric HIV Infection, and Antiretroviral Therapy for HIV Infection. For patient education information, see the Immune System Center and Sexually Transmitted Diseases Center, as well as HIV/AIDS and Rapid Oral HIV Test.
Epidemiology
According to the US Renal Data System (USRDS), HIV-associated nephropathy (HIVAN) accounts for approximately 1% of new end-stage renal disease (ESRD) cases in the United States. HIVAN is observed in patients regardless of the route by which HIV was contracted. HIVAN is observed predominantly among African Americans and is the third leading cause of ESRD among blacks aged 20-64 years.[5, 6] Most patients with HIVAN are young black men, and approximately 50% of patients with HIVAN are intravenous drug abusers.[7, 8] Overall, HIVAN is observed more often in men than in women, with a male-to-female ratio of 10:1. The mean age of persons with HIVAN is 33 years. HIVAN may occur in children.[9]
Pathophysiology
Experiments using transgenic mice have provided perhaps the strongest evidence for a direct role by HIV type 1 (HIV-1) in the development of HIV-associated nephropathy (HIVAN). Researchers created transgenic mice by inserting HIV DNA constructs into the mice's genomes. The mice developed proteinuria and had a histologic picture similar to that observed in patients with HIVAN. A genetic or environmental cofactor that has not yet been identified is required for patients to develop this disease. This unidentified factor might explain the predilection for HIVAN among black persons.[5] The cellular target in the development of HIVAN is probably the renal glomerular and tubular epithelium. Using in situ hybridization and polymerase chain reaction assays to detect HIV-1 DNA and messenger ribonucleic acid (mRNA), investigators have shown that renal glomerular and tubular epithelial cells are productively infected by HIV-1 in patients with HIVAN; this argues strongly for localized replication of HIV-1 in the kidney and for the existence of a renal viral reservoir. Further, circularized viral DNA, a marker of recent nuclear import of full-length, reversetranscribed RNA, has been detected in kidney biopsy samples from patients with HIVAN, suggesting active replication in renal tissue.[10] However, the mechanisms of virus-induced renal injury remain undetermined. Peculiar histopathologic features of HIVAN are the enhanced proliferation and the loss of differentiation markers of glomerular epithelial cells. In one study, HIV-1 infection was shown to kill renal tubular epithelial cells in vitro by triggering an apoptotic pathway involving caspase activation and Fas up-regulation, suggesting that apoptosis of nonlymphoid cells can be directly induced by HIV-1. The net and long-standing glomerular and tubular epithelial cell damage leads to proteinuria, glomerulosclerosis, and tubulointerstitial scarring. The role of cytokines has not been established, and although their presence is not essential for the development of HIVAN, cytokines may modify the progression of infection or a patient's susceptibility to infection. The levels of cytokines are increased in renal biopsy samples from patients with HIVAN.
In one study, mesangial and tubular cell production of interleukin-6 and tumor necrosis factor alpha was shown to be a potent stimulus for HIV-1 expression in HIV-1infected monocytes.[11] Viral replication in response to cytokines may play an important role in the pathogenesis of HIVAN.
Genetics
The reason behind the increased predilection among black persons for the development of HIVassociated nephropathy is not clear.[5] In general, black persons have a higher incidence of other renal diseases (eg, diabetic nephropathy, lupus); therefore, they may have an underlying genetic predisposition to severe renal disease, regardless of the etiology. The type of host response to the HIV infection itself may be what determines whether or not nephropathy develops in a specific individual. Kopp et al studied genetic variants predisposing to idiopathic and HIV-1associated focal segmental glomerulosclerosis (FSGS), and they concluded that genetic variation at the MYH9 locus substantially explains the increased burden of FSGS and hypertensive kidney disease among African Americans. They carried out admixture-mapping linkage-disequilibrium genome scanning on 190 African American individuals with FSGS and 222 controls and identified a chromosome-22 region centered on MYH9, a functional candidate gene expressed in kidney podocytes.[12]
Prognosis
In one study, the rate of progression from the initial presentation to ESRD was 2.5 months in the pre-HAART (highly active antiretroviral therapy) era. With the introduction of HAART in 19961997, the traditional natural history of rapid progression of HIV-associated nephropathy (HIVAN) has been slowed significantly. HAART has been shown to retard the progression of renal disease in persons with HIVAN, and treatment with angiotensin-converting enzyme inhibitors may be beneficial. Among patients with HIV infection and end-stage renal disease receiving hemodialysis, survival has improved significantly compared with the uniformly dismal outcomes in the 1980s.[13]
Clinical Presentation
Patients with HIV-associated nephropathy (HIVAN) typically present with a nephrotic syndrome consisting of nephrotic-range proteinuria (>3.5 g/d), azotemia, hypoalbuminemia, and hyperlipidemia. Edema is uncommon in HIVAN, yet many authors think that this is a characteristic of HIVAN. The salt-losing propensity and high oncotic pressure contributed by marked hypergammaglobulinemia in these patients have been suggested as possible explanations for this puzzling observation.
The CD4+ T-cell count in patients with HIVAN is usually depressed below 200 cells/L, but HIVAN has been reported in patients with higher CD4 counts. The prognosis for renal survival is worse in patients with AIDS, especially if their CD4 count is less than 50 cells/L.
Urinalysis
Routine urinalysis may occasionally reveal findings of nonnephrotic proteinuria in patients being evaluated for other medical conditions. The urinalysis reveals microhematuria, leukocytes, hyaline casts, and oval fat bodies, but no cellular casts. Serum complement levels are normal.
SIADH
Electrolyte abnormalities, such as hyponatremia and hyperkalemia, may be observed in patients with HIVAN and may reflect an increase in total body water (from nephrotic syndrome or syndrome of inappropriate secretion of antidiuretic hormone [SIADH]) or from hyporeninemic hypoaldosteronism, respectively. SIADH may result from concomitant pulmonary infection or from persistent nausea from medications or gastrointestinal disease. Hyporeninemic hypoaldosteronism, a cause of type IV renal tubular acidosis manifesting as hyperkalemia with normal anion gap metabolic acidosis, is much more common when renal insufficiency is present.
Renal Biopsy
The decision to obtain a biopsy sample is somewhat controversial in the general medical community. Even if a patient presents with the classic clinical features of HIV-associated neuropathy (HIVAN), clinical consideration is predictive of the biopsy diagnosis in only 55-60% of patients. Therefore, to distinguish HIVAN from other forms of renal disease (eg, immune complex glomerulonephritis, immunoglobulin-A nephropathy), patients who are seropositive for HIV require a renal biopsy. The typical practice is to obtain a renal biopsy specimen if the patient's daily protein excretion is greater than 1 g.
Histology
Findings from light microscopy of kidney biopsy tissue are diagnostic in most cases. The most common histologic light microscopy finding is a collapsing form of focal segmental
glomerulosclerosis.[14] The glomerular capillary tuft is collapsed (see the first image below) and may be segmentally or globally sclerosed. Visceral epithelial cells are hypertrophied and form a characteristic pseudocrescent in the Bowman space. Tubulointerstitial scarring, atrophy, and marked dilatation of the tubules (microcystic dilatations) are usually present (see the second image below).
Light microscopy with trichrome staining showing the collapse of the glomerular tuft, with segmental glomerular and interstitial sclerosis (bluish staining). The
renal tubules are dilated and filled with proteinaceous material. Light microscopy showing prominent microcystic dilatation of renal tubules filled with proteinaceous material; this finding is characteristic of human immunodeficiency virus (HIV) associated nephropathy, although it may also be observed in chronic glomerulonephritis. Immunofluorescent microscopy helps to identify positive staining for albumin and immunoglobulin G in epithelial cells and for immunoglobulin M, C3, and, occasionally, A in mesangial or sclerotic areas. Electron microscopy reveals wrinkling of the basement membranes, epithelial cell proliferation, and focal foot process effacement. Tubuloreticular structures in the glomerular endothelial cells (consisting of ribonucleoprotein and membrane, the synthesis of which is stimulated by alpha interferon) is highly predictive of HIVAN (see the image below).
Electron microscopy showing a segment of the glomerular basement membrane; foot process effacement (black arrow) and prominent tubuloreticular inclusions (red arrow) are present.
Dose adjustment should be made in patients receiving nucleoside reverse transcriptase inhibitors (NRTI) when the glomerular filtration rate falls below 50 mL/min. Patients receiving nonnucleoside reverse transcriptase inhibitors (NNRTIs) may also receive a dose adjustment when the glomerular filtration rate falls below 50 mL/min. No dose adjustment is required for patients taking protease inhibitors. Some drugs used to treat opportunistic infections in HIV disease may also cause nephrotoxicity or electrolyte abnormalities (see the image below).
Types of electrolyte abnormalities observed with some of the drugs used to treat opportunistic infections in patients with human immunodeficiency virus (HIV). ARF stands for acute renal failure.
Pharmacologic Therapy
Although there are no guiding clinical studies, some experts recommend consideration of therapy in all patients with HIV-associated nephritis (HIVAN). Initiation or adjustment of antiretroviral therapy may be indicated. See Antiretroviral Therapy for HIV Infection for specific information, including recommendations for dosage adjustments for renal insufficiency. Angiotensin-converting enzyme (ACE) inhibitors and corticosteroids have been studied for use in HIVAN. Some reports on pediatric populations suggest that cyclosporine can be effective in reducing proteinuria in persons with HIVAN. The usefulness of cyclosporine therapy for HIVAN warrants further study. Researchers are pursuing several promising therapeutic strategies. Patients who progress to end-stage renal disease (ESRD) require dialysis and consideration of renal transplantation in carefully selected cases.
antiproliferative effect mediated in part by the inhibition of transforming growth factor beta. Use ACE inhibitors if patients do not have hyperkalemia.
Corticosteroids
A number of case reports have suggested that corticosteroids offer some short-term benefit in HIVAN.[19] In one report, results from a pretreatment and posttreatment kidney biopsy suggested that an improvement in renal function was associated with a reduced number of lymphocytes and macrophages infiltrating the interstitium. In another report, of 20 patients who were treated with prednisone, 60 mg/day for 2-11 weeks, followed by a slow taper,[20] 8 patients required maintenance dialysis, 11 died from complications, and 7 were alive and no longer had ESRD after a follow-up of 44 weeks.
Anecdotal reports drawn from small samples of this selected group of patients with HIVAN suggest no extra risk of opportunistic infections. Until larger studies are performed, however, transplantation in persons with HIVAN should be focused on stable patients. In one study, 1- and 2-year actuarial patient survival was 85% and 82%, respectively, and graft survival was 75% and 71%, respectively. Plasma HIV-1 RNA remained undetectable, and CD4 counts remained in excess of 400 cells/L with no evidence of AIDS for up to 2 years. These results were comparable to other high-risk populations receiving kidney transplantation. In a prospective study of kidney transplantation in 150 HIV-infected patients, Stock et al reported 1- and 3-year graft survival rates of 90.4% and 73.7%, respectively. The rejection rate was higher than expected, however, with 1- and 3-year estimates of 31% and 41%, respectively. Living-donor transplants were protective. Before transplantation, all patients had CD4 counts of at least 200/L and undetectable plasma HIV-1 RNA levels while being treated with a stable antiretroviral regimen, and HIV remained well controlled after transplantation.[25] In an analysis of the United Network for Organ Sharing (UNOS) database, Locke et al evaluated 39,501 patients undergoing renal transplantation between January 1, 2004, and June 30, 2006 and found no difference in patient survival among HIV-positive patients versus HIV-negative patients (95.4% vs 96.2%, respectively).[13] However, death-censored 1-year graft survival was significantly lower among HIV-positive patients (87.9% vs 94.6%). Locke et al concluded that with proper donor selection and transplant recipient management, including the avoidance of prolonged cold ischemic time, use of living donors, and determination of optimal immunosuppression dosing before transplant, long-term graft survival comparable to that in HIV-negative patients can be achieved.