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LLTECH 2011

TABLE OF CONTENTS
RESEARCH APPLICATIONS

COSMETOLOGY
CELLS

Page 3
Page 8

DEVELOPMENTAL BIOLOGY Page 13

PLANTS

Page 25

LLTECH 2011

COSMETOLOGY
- In-vivo layer thickness measurement, cell count, epidermis/dermis junction analysis, for ageing and hydration evaluation - Skin model analysis

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LLTECH 2011

En-face skin slicing shows structural and cellular details

Corneocytes

Keratinocyte nuclei

Epidermis

100 m

100 m

Stratum corneum

Stratum spinosum

Melanin papillary caps

Blood vessels

Dermis
Collagen

100 m

100 m

Stratum basale
LLTech 2012 LLTECH 2011

Reticular region
Courtesy of Hopitaux Universitaires de Genve, Genve, Switzerland 4

Vertical reconstructions from en-face slicing: Layer count and measurement

Stack of images -> vertical sections
Keratinocyte nuclei

Skin model
Stratum corneum Stratum spinosum

Dermis
50 m

Human skin (in-vivo)

Evaluation of stratum corneum thickness: 10 m Melanin caps at the epidermis/dermis junction
50 m

Blood vessel
5

LLTECH 2011

Dalimier and Salomon Dermatology 224, 84-92 (2012)

Naevus imaging

Dermal papillae

50 m

500 microns
100 m

Hair follicule

LLTECH 2011

3D views
Epidermis (separated by sodium bromide) Fingerprints (in-vivo)

Sweat duct
Wrinkle Stratum corneum Stratum spinosum
100 m

50 m

Corneocytes

Reconstructed vertical slice

LLTECH 2011

Dalimier and Salomon Dermatology 224, 84-92 (2012) 7

CELL IMAGING
- Cell count in native tissues and scaffolds - Volume and shape analysis

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Adipocytes in human tissue

200 m

200 m

200 m

9 m depth

27 m depth

60 m depth

Reconstructed depth slice

50 m

LLTECH 2011

Effect of fibrosis on adipocyte shape

Reconstructed vertical views of adipocytes in gel No fibrosis 3D modelling of adipocyte

50 m

With fibrosis

50 m

LLTECH 2011

Courtesy of Centre de Recherche des Cordeliers and ESPCI, Paris, France 10

Cell scaffold
Scaffold en-face view

Scaffold reconstructed depth slice

100 m

Hepatocytes nuclei are visible

100 m

LLTECH 2011

Courtesy of University of Toronto, Canada 11

Individual cells
Isolate collagen cells

100 m

LLTECH 2011

Courtesy of Universit de Reims, France 12

DEVELOPMENTAL BIOLOGY
Time series analysis Reconstructed depth slices and 3D imaging

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13

Developmental biology: Drosophila

200 m

LLTECH 2011

Courtesy of ESPCI, Paris, France 14

In vivo imaging of drosophila melanogaster: automated time series over 72 hours

Prepupa (0-2 h)

Transition to pupal stage (24 h)

Pupal stage (48 h)

Advanced pupal phase before eclosion (72 h)

LLTECH 2011

Courtesy of ESPCI, Paris, France 15

In vivo imaging of drosophila melanogaster 4 days

100 um

LLTECH 2011

Courtesy of ESPCI, Paris, France 16

Xenopus Laevis

Cartilage Pigmented cells

Otic vesicle

100 um

LLTECH 2011

Courtesy of Muse National dHistoire Naturelle, Paris, France 17

Xenopus Laevis: back/tail

30 m depth

60 m depth
100 um

Epithelial cells

Myoseptum

Neural cells

LLTECH 2011

Courtesy of Muse National dHistoire Naturelle, Paris, France 18

Xenopus Laevis
Reconstructed vertical slices from stacks of images

50 um

LLTECH 2011

Courtesy of Muse National dHistoire Naturelle, Paris, France 19

Xenopus Laevis eye: en face slices

Surface

10 m depth

20 m depth

30 m depth

40 m depth

50 m depth

60 m depth

70 m depth

80 m depth

140 m depth

180 m depth

230 m depth
200 um
Courtesy of Muse National dHistoire Naturelle, Paris, France 20

LLTECH 2011

Copepod

100 um

LLTECH 2011

Courtesy of Laboratoire de Rgulation des Signaux de Division, EA4479, University of Lille 21

Zebra fish eye

En face slices

100 m

10 m depth

20 m depth

45 m depth

Reconstructed depth slice

100 m

LLTECH 2011

Courtesy of Inserm, Paris, France 22

Zebrafish brain female, 1 year old

Reconstructed view from stack of images

50 um

LLTECH 2011

Courtesy of Laboratoire Hubert Curien, Saint Etienne

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C. Elegans Fast identification of anatomy

15 m depth

30 m depth
ovocytes

Body wall - cuticle

gonad arm

intestine

3D reconstruction
uterus
100 m

100 m

LLTech 2012 LLTECH 2011

Courtesy of ENS, Paris, France 24

PLANTS
- High resolution reconstructed depth slices and 3D imaging

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LLTECH 2011

25

Leaf veins fresh leaf sample

Veins

500 m

Fibers
100 m

26
LLTECH 2011
Courtesy of ESPCI, Paris, France 26

Apple

Wax Skin

Water

Flesh

500 m

100 m

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LLTECH 2011
Courtesy of ESPCI, Paris, France 27

Sorghum : cross section

Wide field en face 14 mm x 15 mm

Courtesy of Plate-forme d'Histocytologie et d'Imagerie Cellulaire Vgtale (PHIV), Montpellier RIO Imaging

LLTECH 2011

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Sorghum cross section: native field view

Reconstructed depth slices 500 m sample thickness

800 m x 800 m en face field

LLTECH 2011

Courtesy of Plate-forme d'Histocytologie et d'Imagerie Cellulaire Vgtale (PHIV) Montpellier RIO Imaging

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Rice: hypocotyle

Wide field en face 5mm x 2mm

Reconstructed depth slice, 200um penetration depth

LLTECH 2011

Courtesy of Plate-forme d'Histocytologie et d'Imagerie Cellulaire Vgtale (PHIV), Montpellier RIO Imaging 30

Contacts

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LLTech Ppinire Paris Sant Cochin 29 rue du Faubourg Saint Jacques 75014 Paris Phone: +33 1 82 72 61 25

www.lltechimaging.com contact@lltech.fr

www.lltechimaging.com LLTECH 2011

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