PLP 3002/5005 Fundamentals of Plant Pathology/General Plant Pathology (Fall 2011)

COURSE AND LABORATORY INSTRUCTOR: Dr. Brantlee Spakes Richter Office: 2519 Fifield Hall Phone: 352-273-2014 Email: bsr@ufl.edu Office Hours: Monday & Thursday 2:00-4:00pm, or by appointment TEACHING ASSISTANT: Office: 2509 Fifield Hall Phone: 352-870-5577 Email: alinascampbell@ufl.edu Office Hours: by appointment COURSE(S): Fundamentals of Plant Pathology (PLP 3002C, sections 3931 and 3933) and General Plant Pathology (PLP 5005C, sections 3937 and 3938) are taught concurrently. Undergraduates are encouraged to enroll in PLP 3002, whereas Plant Pathology graduate students and DPM students are encouraged to enroll in PLP 5005 so that they can receive graduate credits for the class. Students enrolled in PLP 5005 will be required to complete two additional projects beyond the requirements of PLP3002 (described below). PREREQUISITE: BOT 2010 or BSC 2010 CREDITS: 4 CLASS TIME/LOCATION: Lectures: 2316 Fifield Hall, Tuesday and Thursday period 4 @ 10:40-11:30 am Laboratories: 2306 Fifield Hall, Tuesday (sec. 3931/3937) or Wednesday (sec. 3938/6959) Periods 6-8 @ 12:50-3:50 pm INTRODUCTION: Plant pathology is the science of plant diseases, the microorganisms that cause them, and their interactions. The ultimate goal of plant pathologists is to reduce the losses caused by plant diseases, thereby increasing plant quality and quantity. Plant diseases are caused by many of the same types of organisms that cause diseases in animals and humans and, as such, many of the principles that apply to animal and human medicine apply to plant diseases. The objective of this course is to introduce students to the many different types of plant pathogens, their basic biology, examples of the types of disease they cause, and the basic principles and concepts of their development, spread, and management. TEXT: Plant Pathology 5th Ed. (2005) by G. N. Agrios, Elsevier Academic Press, Inc. (The 4 edition of this book is also acceptable; published by Academic Press, Inc. NY.) ATTENDANCE: Attendance is not mandatory, but is highly advisable. You are expected to participate in every class and laboratory, and there will be daily quizzes and/or class activities which will contribute to your grade in the class. Missed activity grades may only be made up for excused absences, at the discretion of the instructor. Absences due to personal planning (ie, planning to be somewhere other than class during class time) will not be excused, and missed points may not be made up. There will be no make-up lab sessions . LABORATORY: The laboratory will emphasize principles and concepts of plant pathology through demonstrations and hands-on exercises using living organisms and prepared specimens. Labs will typically include an introduction to the lab exercise, work with demonstration materials, and a deliverable
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to be handed in either at the end of the session or the beginning of the subsequent session. It is advised that you keep a lab notebook for all labs. Your lab notebook will not be graded, but a well-kept notebook will be highly beneficial to you in preparing lab assignments and studying for exams. PLP 5005 Requirements: Students taking the class for graduate credit will have one individual and one group assignment, in addition to the set of requirements for the undergraduate course: 1. Each student will be required to select a set of 2-3 journal articles on a single topic, and to write a critique of the selected articles. Articles must be published in international peer-reviewed journals, and should be selected to present contrasting approaches to a single research question. Your critique will focus on the differing approaches that each research lab takes, discuss the advantages and disadvantages of each set of methods used, and make recommendations for future research on the topic. 2. Graduate students as a group will conduct an experiment on the topic of their choosing (subject to instructor approval). All students may work together, or you may split into no more than two groups, to accommodate schedules and topical areas of interest. There will be three deliverables associated with the group project: (1) a research proposal, (2) a journal-style article, and (3) an in-class presentation. Topics selected for individual assignments may be used as a starting point for the group experiment(s). Further details on both assignments will be discussed in class. EXAMS AND GRADING: There will be three lecture exams (two mid-terms and one final) and one laboratory exam. The final lecture examination (Exam Group 12B) is scheduled for 10:00 AM 12:00 PM, Monday, December 12, in room 2316 Fifield. The lab exam will be held during the last regularly scheduled laboratory session, December 6 and 7. Grading for PLP3002: There will be a total of 500 possible points earned for PLP3002 students, with 100 points from each of the exams (3 lecture and 1 lab), and 50 points each from class and lab participation assignments/quizzes. Grading for PLP5005: Graduate students will be responsible for the same 500 points, plus two additional assignments worth 150 points, for a total of 650 possible points. Component Exam 1 Exam 2 Final Exam Class Participation Lab Assignments Lab Exam Critique Paper Group Project Points 100 100 100 50 50 100 50 100 PLP3002 20% 20% 20% 10% 10% 20% PLP5005 15.4% 15.4% 15.4% 7.7% 7.7% 15.4% 7.7% 15.4%

ACADEMIC HONESTY In 1995 the UF student body enacted a new honor code and voluntarily committed itself to the highest standards of honesty and integrity. When students enroll at the university, they commit themselves to the standard drafted and enacted by students. In adopting this honor code, the students of the University of Florida recognize that academic honesty and integrity are fundamental values of the university community. Students who enroll at the university commit to holding themselves and their peers to the high standard of honor required by the honor code. Any individual who becomes aware of a violation of the honor code is bound by honor to take corrective action. The quality of a University of Florida education is dependent upon community acceptance and enforcement of the honor code. The Honor Code: We, the members of the University of Florida community, pledge to hold ourselves and our peers to the highest standards of honesty and integrity. On all work submitted for credit by students

at the university, the following pledge is either required or implied: On my honor, I have neither given nor received unauthorized aid in doing this assignment. The university requires all members of its community to be honest in all endeavors. A fundamental principle is that the whole process of learning and pursuit of knowledge is diminished by cheating, plagiarism and other acts of academic dishonesty. In addition, every dishonest act in the academic environment affects other students adversely, from the skewing of the grading curve to giving unfair advantage for honors or for professional or graduate school admission. Therefore, the university will take severe action against dishonest students. Similarly, measures will be taken against faculty, staff and administrators who practice dishonest or demeaning behavior. Students should report any condition that facilitates dishonesty to the instructor, department chair, college dean or Student Honor Court. (Source: 2007-2008 Undergraduate Catalog) It is assumed all work will be completed independently unless the assignment is defined as a group project, in writing by the instructor. This policy will be vigorously upheld at all times in this course. SOFTWARE USE All faculty, staff and students of the university are required and expected to obey the laws and legal agreements governing software use. Failure to do so can lead to monetary damages and/or criminal penalties for the individual violator. Because such violations are also against university policies and rules, disciplinary action will be taken as appropriate. CAMPUS HELPING RESOURCES Students experiencing crises or personal problems that interfere with their general wellbeing are encouraged to utilize the universitys counseling resources. Both the Counseling Center and Student Mental Health Services provide confidential counseling services at no cost for currently enrolled students. Resources are available on campus for students having personal problems or lacking clear career or academic goals, which interfere with their academic performance. The Counseling Center is located at 301 Peabody Hall (next to Criser Hall). Student Mental Health Services is located on the second floor of the Student Health Care Center in the Infirmary. University Counseling Center, 301 Peabody Hall, 392-1575,www.counsel.ufl.edu Career Resource Center, CR-100 JWRU, 392-1602, www.crc.ufl.edu/ Student Mental Health Services, Rm. 245 Student Health Care Center, 392-1171, www.shcc.ufl.edu/smhs/ - Alcohol and Substance Abuse Program (ASAP) - Center for Sexual Assault / Abuse Recovery & Education (CARE) - Eating Disorders Program - Employee Assistance Program - Suicide Prevention Program STUDENTS WITH DISABILITIES The Disability Resource Center coordinates the needed accommodations of students with disabilities. This includes registering disabilities, recommending academic accommodations within the classroom, accessing special adaptive computer equipment, providing interpretation services and mediating facultystudent disability related issues. 0001 Reid Hall, 392-8565, www.dso.ufl.edu/drc/ THE INSTRUCTOR RESERVES THE RIGHT TO CHANGE OR MODIFY INFORMATION PROVIDED IN THE SYLLABUS. CLASS ANNOUNCEMENTS SUPERSEDE SYLLABUS STATEMENTS

COURSE LECTURE SCHEDULE Fall 2011

(Numbers refer to chapter and page no. of the reading assignment th in Agrios 5 edition text)

Date Aug 23: Aug 23: Aug 25: Aug 30: Sept 01: Sept 06: Sept 08: Sept 13: Sept 15: Sept 20: Sept 22: Sept 27: Sept 29: Oct 04: Oct 06: Oct 11:

Topic Introduction to Plant Pathology, terminology Chapt 1: 3-27, Chapt 1: 71-75 (12.50-1.30 pm) Abiotic diseases, Chapt 10: 357-383 Introduction to Plant Pathology, diagnosis Separate handout Introduction to Plant Pathology, history Chapt 1: 29-53 and 65-69 Introduction to Plant Pathology, disease development and cycles Chapt 2: 77-89 and 96-102 Plant Pathogenic Fungi/Diseases caused by fungi Chapt 11: 383-404 Plant Pathogenic Fungi/Diseases caused by fungi Chapt 11: 383-404 Plant Pathogenic Fungi/Diseases caused by fungi Chapt 11: 383-404 Plant Pathogenic Bacteria/Diseases caused by bacteria Chapt 12: 615-627 (Jones) Plant Pathogenic Bacteria/Diseases caused by bacteria Chapt 12: 615-627 (Jones) Plant Pathogenic Bacteria/Diseases caused by bacteria Chapt 12: 615-627 (Jones) Plant Pathogenic Viruses/Diseases caused by viruses Chapt 14: 724-756 Plant Pathogenic Viruses/Diseases caused by viruses Chapt 14: 724-756 Plant Pathogenic Viruses/Diseases caused by viruses Chapt 14: 724-756 Plant Pathogenic Nematodes Chapt 15: 826-836 (Crow) Plant Pathogenic Nematodes Chapt 15: 826-836 (Crow)

Oct 13: Oct 18: Oct 20: Oct 25: Oct 27: Nov 1: Nov 3:

Effects of pathogens on plant physiology Chapt. 3: 106-121 How pathogens attack plants Chapt 5:176-203 Genetics of Plant disease Chapt 4: 125-174 Genetics of Plant disease Chapt 4: 125-174 Plant defenses - structural Chapt 6:210-217 Plant defenses - biochemical Chapt 6:217-236 Midterm exam Environmental factors that affect infectious diseases Chapt 7: 249-265 Plant disease epidemiology Chapt 8: 266-289 Chemical control of plant diseases Chapt 9: 295-348 Cultural control of plant diseases Chapt 9: 295-348 Forum discussion about careers in Plant Pathology Chapt 1: 60-65 Biocontrol of plant diseases Chapt 9: 295-348 No class (Thanksgiving Week) Integrated approach to vegetable disease management Presentations of class projects by grad students last class period 10:00AM-12:00PM final exam in 2316

Nov 8: Nov 10: Nov 15: Nov 17: Nov 22: Nov 24: Nov 29: Dec 01: Dec 06: Dec 12:

PLP 3002/5005 LABORATORY SCHEDULE Fall 2011

Aug 23-24: Abiotic diseases lecture Introduction to PLP Lab and literature Tour on campus, microscope use, moist chamber Symptoms and Signs of Plant Diseases (397-402) Aug 30-31: Isolations of bacteria and fungi Going through a simple key

(Numbers in parentheses are pages in Agrios 5 edition) McGovern van Bruggen

th

van Bruggen Rollins, Benny

Sept 6-7: Soilborne diseases by fungi (404-421; 522-552; 593-603): Microscopic observations Oomycota and Myxomycota ( Phytophthora, Pythium, Plasmodiophora) Ascomycetes and Basidiomycetes (Fusarium, Verticillium, Sclerotium, Rhizoctonia) Sept 13-14: Foliar diseases by fungi (439-445; 448-472; 483-500; 501-519) Macroscopic and microscopic observations Oomycota ( Phytophthora, Peronospora) Ascomycetes ( Venturia, Guignardia, Colletotrichum, Botrytis, Erisyphe; Alternaria) Sept 20-21: Bacterial diseases (616-675) Microcope (streaming, Gram staining, KOH) (Erwinia, Pseudomonas, Xanthomonas, Agrobacterium, Ralstonia) Sept 27-28: Foliar diseases by fungi (445-447; 562-593) Macroscopic and microscopic observations Basidiomycota (Puccinia,Ustilago, Tilletia, Taphrina) Oct 4-5: Plant Viruses inoculation of plant viruses I. Mechanical inoculation II. Insect inoculation aphid inoculation of potyviruses Oct 11-12: Nematodes (overview 826-837, 838-870) Microscopic observations, demonstration of isolation techniques (Meloidogyne, Heterodera,Pratylenchus, Radopholus) (Trichodorus, Aphelenchoides) Oct 18-19: Plant Viruses virus symptoms I. Examine results from previous laboratory II. Role of symptoms in diagnosis of viral diseases Oct 25-26 : Intro molecular techniques 1 (protein-protein interaction assays) Nov 1-2 Intro molecular techniques 2 (polymerase chain reaction, rolling circle amplification)

Rollins, Benny

Jones

Rollins

Polston, Capobianco

Crow

Polston, Capobioanco

Song Song

Nov 9-10 Postharvest diseases (434-438; 507-510; 546-561; 656-662) Macroscopic and microscopic observations (Rhizopus, Mucor, Monilia, Aspergillus, Penicillium, Sclerotinia, Erwinia) Nov 15-16: Last lab period Nov 22-23: No lab (Thanksgiving week) Nov 29-30: Lab review Dec 6: Lab exam in Room 2306, 12:50 AM to 3.50 PM Dec 7: Lab exam in Room 2306, 12:50 AM to 3.50 PM

Bartz, Spiceland