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Population
All organisms of one species in a habitat/area/place/at one time;
Community
A collection of different populations of species living in the same ecosystem
Ecosystem
The community and the abiotic/physical environment;
Ecological Niche
The niche of an organism refers to its biotic role (what it feeds on and what may feed on it) and its abiotic role (where it lives in the habitat in terms of the abiotic conditions it requires).
Transect
Line (through habitat) along which organisms are sampled/ measurements are taken; Should be used when there is a change in the habitat/environment;
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How ATP is produced using light in LDP
1 Light (energy) excites/raises energy level of electrons in chlorophyll; 2 Electrons pass down electron transfer chain; 3 (Electrons) reduce carriers/passage involves redox reactions; 4 Electron transfer chain / role of chain associated with chloroplast membranes / in thylakoids / grana; 5 Energy released / carriers at decreasing energy levels; 6 ATP generated from ADP and phosphate/Pi / phosphorylation of ATP;
Why does the inhibition of electron transfer decrease the uptake of carbon dioxide?
No ATP produced during electron transfer chain No reduced NADP produced
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when electrons (from transport chain) and H+ combine with NADP; H+ from photolysis
Explain why the increase in dry mass is less than the amount of 6 Carbon sugars
1 2 3 4 some hexose/biomass/eq. used in respiration; CO2 produced (is lost to air); some parts of the plant are eaten; some parts lost to decomposers / in leaf fall;
Suggest why the deep ocean has a lower productivity than the shallow sea.
Fewer nutrients available; Limited light penetration; No organic matter from terrestrial sources; Other limiting factor explained
Reasons for the difference in the amount of light energy reaching the plants, and the energy in biomass of these plants
light reflected; light misses chlorophyll/chloroplast/transmission through leaf; wrong wavelength; respiration (by primary producer); inefficiency of photosynthesis;
Why is the concentration of carbon dioxide different at different heights above ground in a forest?
High concentration of carbon dioxide linked darkness No photosynthesis in the dark Plants still respire in the dark In light, the rate of photosynthesis is greater than that of respiration Decrease in carbon dioxide concentration with light At ground level, fewer leaves/ less photosynthesising tissue/ less light
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Why is ATP useful in many biological processes??
Releases energy in small manageable amounts Broken down in one step Makes energy available rapidly Adds phosphate Makes phosphorylated substances more reactive Can be reformed
Link Reaction
Pyruvate reacts with coenzyme A; To produce acetyl coenzyme A / acetyl coA; And carbon dioxide produced; NAD is reduced / reduced NAD is formed; Acetylcoenzyme A combines with a 4C molecule (in the Krebs cycle);
Electrons transferred down the electron transport chain Provides energy to take protons into space between membranes Protons pass back through membrane into the matrix Energy used to combine ADP with phosphate to form ATP
Measuring rate of respiration using a respirometer, why does the coloured liquid move?
Oxygen taken up by organism Carbon dioxide absorbed by substance (named) Decrease in pressure
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Why efficiency of energy transfer changes between trophic levels
1 Some light energy fails to strike/is reflected/not of appropriate wavelength; 2 Efficiency of photosynthesis in plants is low/approximately 2% efficient; 3 Respiratory loss / excretion / faeces / not eaten; 4 Loss as heat; 5 Efficiency of transfer to consumers greater than transfer to producers/approximately 10%; 6 Efficiency lower in older animals/herbivores/ primary consumers/warm blooded animals/homoiotherms; 7 Carnivores use more of their food than herbivores;
Why is the energy efficiency of single celled producers to primary consumers sometimes higher than usual?
Single celled organisms are more digestible as they contain less cellulose All of producer eater/ parts of a plant are not eaten Less energy is lost from the producer by heat or respiration compared to a plant
limiting factor
Bioaccumulation
pest take in pesticide; predators eat large numbers of pests; bioaccumulation idea / pesticide cannot be excreted / remains in the body / stored in fat / not broken down
Nitrogen fixing bacteria / named e.g.; in root nodules (of legumes); convert nitrogen to ammonium / organic compounds (in legume); released on decomposition; and converted to nitrates; less need for fertiliser;
Why would there be a higher total yield if you grow many different species, rather than one species??
Different species have different root lengths; Mineral ions/water can be obtained from different depths; Shade plants grow as well as those needing full sunlight; Pests tend to be specific; Pests will not destroy total crop in mixed system; Less fallow time;
Describe and explain the effect of adding fertiliser on the biomass of the plants.
increase growth of both weeds and cereal crop; inorganic nutrients/minerals not a limiting factor / more inorganic nutrients available
Explain why the application of herbicide increases the final yield of the crop.
removal of competition by weeds
What factors do you need to consider when doing an experiment about crops
Yield Animals Cost of fertiliser Method/frequency of application
Give one advantage of using natural fertiliser rather than artificial fertiliser
Contains a wider range of elements Production of artificial fertiliser energy-consuming Less leaching/slow release of nutrients
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How does deforestation and burning vegetation affect carbon dioxide concentration
Carbon dioxide concentration increases Less vegetation so less photosynthesis Less carbon dioxide removed Combustion releases carbon dioxide
Difference between the way detritivores and decomposers obtain their nutrients
Decomposers secrete enzymes / onto organic matter/ food/ extracellular breakdown; Detritivores ingest / eat/ take in organic matter/food first;
Eutrophication
growth of algae / plants increased; death of submerged algae / plants; more bacteria / decomposers / decomposition; respiration uses oxygen; aerobic animals die
Used to synthesise amino acids Advantanges of Inorganic Fertilisers easy to handle / apply / transport / store; known chemical content / can supply specific needs; easy to control mass that is added / less mass needed; releases ions / nutrients quickly / soluble Disadvantages of Inorganic fertilisers expensive / more readily leads to eutrophication / environmentally damaging / uses resources to make it / does not add to soil structure / lacks some nutrients
How does less ploughing mean more carbon is stored in the soil
Less oxygen can enter the soil (from the air); For saprophytes / soil microorganisms / bacteria / fungi / decomposers / correctly named soil organisms; For use in aerobic respiration; Less breakdown of organic matter / humus / dead plants / dead animals / other e.g.; Less carbon dioxide released / formed;
Ploughing increases the activity of nitrifying bacteria in the soil. Why? And how does this benefit plants?
Oxygen enters the soil / use of oxygen; Nitrifying bacteria are aerobic; Ammonia / ammonium ions to nitrite; Nitrite to nitrate; Nitrate is absorbed / used by plants; To make named organic-N e.g. protein / amino acids / DNA / ATP / NAD(P) / chlorophyll; Increased yield / growth;
Why do plants grow more slowly, getting nitrogen from nitrogen-fixing enzymes, rather than from fertiliser??
ATP is required for nitrogen fixing/ reducing nitrogen Less ATP is available for growth/protein synthesis So greater rate of respiration required to make up for this
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Succession
change in community over time; either due to environmental / abiotic factors / named abiotic factor; or conditions change due to species present
Climax Community
stable community / no further succession / final community
Description of Succession
1. 2. 3. 4. 5. 6. 7. 8. Colonisation/pioneering Microscopic plants at start Death/decomposition Named change in environment e.g. increase in organic matter/stabilisation New species colonise once there is a change Increase in biodiversity Increase in total biomass/more niches Increase in nutrient availability
9. Change from more extreme conditions/ more stability 10. Climax community
Why is it an advantage to have seeds that germinate better when temperature fluctuates?
Bare soil temperature fluctuates So theres more bare soil at the start of succession when there is few plants
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How do you know if 2 alleles are from the same gene??
The frequencies add up to 1
Recessive Allele
Only expressed in phenotype if homozygous
Co-Dominant Allele
Both expressed in phenotype if both are present
Why observed phenotype ratios are not always same as theoretical ratios
chance related to mating; random fusion of gametes; small sample size; differential mortality
Natural Selection
variation between members of population/species; predation/disease/competition results in differential survival; some have adaptations that favour survival; survive to reproduce/have more offspring/ pass on their alleles/genes; produces changes in frequency of allele /gene pool/ genotypes/phenotypes;
B5 Model Answers 9
Kinesis
random movements = 1 mark, e.g. degree of turning / number of turns depends on strength of stimulus / on temperature / allow specific reference to more turning at 35 than at 30 / non-directional stimulus / response
Reflex
rapid response to stimulus; which is automatic / involuntary / not under conscious control;
Importance of reflexes
avoids damage to tissues; role in learning / homeostasis; posture / balance; escape from predators; finding food / mate;
in carotid / aortic bodies / medulla; (more) impulses to cardiac centre / medulla; (more) impulses (from medulla) along sympathetic nerve; to SAN increasing heart rate.
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Why Myelinated faster than non-myelinated
non-myelinated depolarisation occurs along whole length of whole membrane; myelinated depolarisation only occurs at nodes (of ranvier); impulse jumps from node to node / salutatory conduction;
Nervous vs Hormonal
nervous system electrical impulses, hormonal only chemicals; use neurones, use of blood; localised, widespread response; short-lived, long-lasting; rapid, slow response;
Depolarisation / reduced P.D. / 70 to 40 mV; to threshold; by local currents; increased permeability of membrane to sodium ions / sodium gates open; sodium ions enter; by diffusion; positive pd inside / eq via figures; then potassium gates open / permeability to potassium ions increases; potassium ions leave; by diffusion (ONCE only); resting potential re-established;
Automatic;
does not necessarily involve passage to brain / only spinal cord; same pathway used each time; higher brain centres not involved / no thinking;
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Role of Ca2+ in contraction of myofibril
calcium ions bind to / move tropomyosin; to reveal binding sites on actin; allowing myosin (heads) to bind to actin / actinmyosin cross bridge formed; activates ATPase / energy released from ATP;
Role of Phosphocreatine
phosphocreatine allows regeneration of ATP under anaerobic conditions;
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Homeostasis
maintaining a constant internal environment
shunt vessels; diversion of blood to core / specified organ / less blood to skin; muscular contraction / shivering generates heat via respiration; release of thyroxine / adrenaline; increase in metabolic rate / respiration; correct reference to negative feedback mechanisms;
Insulin
increase in blood sugar, (more) insulin secreted; binds to (specific) receptors on (liver / muscle) cells; leads to more glucose entering cells / activates carrier proteins / opens protein channels / increased permeability to glucose; glucose leaves the blood; insulin activates enzymes (in liver); glucose entering cell converted to glycogen / glycogenesis;
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How structure of DNA linked to function
sugar - phosphate backbone gives strength; (coiling gives) compact shape; sequence of bases allows information to be stored; long molecule stores large amount of information; information can be replicated / complementary base pairing; (double helix protects) weak hydrogen bonds / double helix makes molecule stable prevents code being corrupted; chains held together by weak hydrogen bonds; chains can split for replication / transcription
OR OR
tRNA folded versus mRNA straight; tRNA fixed length versus mRNA variable length
Role of tRNA
anticodon complementary to codon / reads message on mRNA; specific amino acid; carried / transferred (to ribosome); correct sequence of amino acids along polypeptide;
Mutagenic Agents
high energy ionized particles/X-rays/ultraviolet light/high energy radiation/uranium/plutonium/gamma rays/tobacco tar/ caffeine/pesticides/mustard gas/base analogues/free radicals;
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Stem Cells cells that can divide to form other types of cell/undifferentiated;
Will replace themselves/keep dividing/replicate;
cells are totipotent / can develop into any cell type; different genes expressed; related to relative concentration of growth regulators; different enzymes / proteins determine tissue formed;
Effect of siRNa
single siRNA strand; binds to mRNA; by complementary pairing; enzyme breaks down mRNA; prevents transcription (of certain genes);
Malignant Tumour
cell division by mitosis; tumour cells growth abnormal / continuous / uncontrolled; tumour cells spread / invade other tissues / form secondary tumours / metastasis; via blood / lymph system;
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How to transfer human gene to plasmid
use of restriction endonuclease/enzyme; same for human and plasmid DNA; sticky ends; ligase used to insert/join human to plasmid DNA;
Using a vector/plasmid/virus; Bacteria reproduce rapidly; Producing many copies of (inserted) gene; Bacteria can be grown on a large scale/ in industrial fermenters; To get a lot of the product of the gene;
Plasmid
Circular loop of DNA; separate from main bacteria DNA; contains only a few genes;
Gene Therapy
introduction of healthy gene / replacement of defective gene
PCR
Heat (DNA) to 95oC, to separate strands/break hydrogen bonds/denature; Cool to 40oC, to allow primers/nucleotides to bind; Primers provide starting point for copying/prevent strands rejoining; Heat to 70oC, optimum for polymerase/enzyme; DNA polymerase then joins nucleotides together;
Uses of PCR
replication of DNA from crime scene / tissue sample / for DNA sequencing / gene cloning
Vector
means of getting new DNA into cell / host / gene carrier
DNA Replication
DNA strands separate / hydrogen bonds broken; Parent strand acts as a template / copied / semi-conservative replication; Nucleotides line up by complementary base pairing; Role of DNA polymerase;
DNA Fingerprinting
1. DNA is cut; 2. using restriction enzyme; 3. use electrophoresis; 4. separates according to length / mass; 5. southern blotting / transfer to (nylon) membrane; 6. make single-stranded; 7. apply probe; 8. radioactive / fluorescent; 9. reference to tandem repeats / VNTRs / minisatellites; 10. autoradiography (if radioactivity used);
Gene Probe
Strand of DNA; Short strand / up to 20 bases long; With base sequence that is complementary to part of target gene; Radioactive labelling / fluorescent labelling;
Why deletion cause more disruption than substitution deletion causes frame shift / alters base sequence (from deletion); changes many amino acids / sequence of amino acids (from deletion); substitution alters one codon / triplet; one amino acid altered / code degenerate / same amino acid coded for; How to make radioactively labelled strands visible after electrophoresis transfer onto nylon / filter sheet; X-ray / photographic film / (auto) radiography; How genes are extracted and then inserted into bacteria 1 (cut out gene using an) endonuclease / restriction enzyme; R. cuts gene 2 reference to specificity / recognition site; 3 sticky ends; 4 use endonuclease / restriction enzyme (to cut plasmid/virus); 5 plasmid/virus; 6 (spliced) by ligase; 7 method of introducing vector e.g. micropipette / shock treatment / / calcium chloride/ions / transformation / tungsten bullet;
Evaluating data
Is there an overlap in ranges? What biotic and abiotic factors are there? What correlation is there? Correlation does not indicate a causal effect