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LE Cell
The LE cell is a neutrophil that has engulfed the antibody-coated nucleus of another neutrophil. LE cells may appear in rosettes where there are several neutrophils vying for an individual complement covered protein.
Genetic Associations
HLA s are loci on genes that code for certain ! chain on the MHC complex HLA-DR2 HLA-DR3 HLA-DQB1 Involved in mediating production of antibodies to ds-DNA
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Symptoms
Non-specific:
Fatigue Weight loss Malaise = generally feeling ill Fever Anorexia (over time) Arthritis
90% of patients experience arthritic symptoms Symmetrical Appears in hands, wrists, and knees mainly
Skin Manifestations
Malar or Butterfly Rash Discoid Rash Stimulated by UV light Skin manifestations only appear in 30-40% of lupus patients.
Renal Failure because of Glomerulonephritis is the leading cause of death among lupus patients.
Normal
Glomerulonephritis
3/27/12
Main Pathology
The plasma cells are producing antibodies that are specific for self proteins, namely ds-DNA Overactive B-cells Suppressed regulatory function in T-cells Lack of T-cells Activation of the Complement system
Overactive B-cells
Estrogen is a stimulator of B-cell activity
Lupus is much more prevalent in females of ages 15-45
Height of Estrogen production
T-cell Malfunctions
Fc region switch
" ! #$ Leads to malfunction in signaling and decreased IL-2 production
3/27/12
IgG Pathogen
IgG is the most pathogenic because it forms intermediate sized complexes that can get to the small places and block them.
3/27/12
DNA is the Primary Auto - Antigen DNA is the main antigen for which antibodies are formed. Extracellular DNA has an affinity for basement membrane where it is bound by autoantibodies. Classical thickening of the basement membrane
Testing
ESR Urinalysis Complement Test
Tests levels of C3, C4, CH50 Low levels indicates possible presence of disease
FANA
ELISA Test
Generally test for:
ds-DNA antibodies Antihistone antibodies
Binds to DNA, major constituent of chromatin
3/27/12
Ouchterlony Test
Used to determine immunological specificity Rules out a false positive Shows the serum does or does not have antinuclear antibodies
Summary
Lupus = Autoimmunity
Systemic and affects connective tissue